PROCESS BIOCHEMISTRY
·
2024
·
IF 3.7
Fengyu Jiang,Jinhong Zhong,Huizhong Hu,Yufu Li,Yongguang Bi,Fansheng Kong
Sugarcane leaves are a common waste product of sugarcane but contain various active components. To make full use of the value of sugarcane leaves, it shall be urgently developed as a sustainable resource. To compare the differences in structure and activity between acidic sugarcane leaf polysaccharides (ASLs) extracted by different extraction methods, ASLW and ASLM polysaccharides were extracted using hot alkali extraction (HAE) and microwave-assisted hot alkali extraction (MHAE), respectively. The physicochemical properties were analyzed with high-performance liquid chromatography, scanning electron microscopy, infrared spectroscopy and X-ray diffraction. Then, the antioxidant, hypolipidemic, hypoglycemic and antibacterial activities of ASLW and ASLM were evaluated by the inhibition of oxygen free radicals, the inhibition of α-glucosidase and α-amylase, the binding ability with bile salts and the filter paper method. Molecular weight and monosaccharide composition analyses showed that ASLW (142.23 kDa) and ASLM (98.86 kDa) were heteropolysaccharides with a wide range of monosaccharide types and molar ratios. ASLM is an amorphous polysaccharide with stronger stability. Also in vitro bioactivity analysis showed that ASLM had stronger antioxidant, hypolipidemic, hypoglycemic and antibacterial effects than ASLW. As a sustainable active substance, the use of ASLM will benefit the pharmaceutical, industrial, agricultural, and food industries.
研究领域:
农业科学
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药学
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食品科学
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材料科学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2021
·Vol. 57
·期 1
·78-91
·
IF 3.713
Xiangna Chang,Xuefeng Chen,Pin Gong,Wenjuan Yang,Lan Wang,Ning Liu,Yao Su,Yuanyuan Zhao
Summary The anti-fatigue effects and structure-activity relationship of apple polysaccharides (APPs), the active components in apple pomace, remain unknown. We examined the effects of different extraction methods on polysaccharide structures and anti-fatigue activities. Additionally, the structural and biochemical properties of APPs prepared under acidic (ACAPP) or alkaline (ALAPP) conditions, were characterised. ACAPP and ALAPP were acidic heteropolysaccharides primarily comprising arabinose, rhamnose, galactose, glucose, xylose and galacturonic acid. The molecular weights of ACAPP and ALAPP were 110 kDa and 89.6 kDa, respectively. ALAPP had more branched chains than ACAPP and showed stronger free radical-scavenging and anti-fatigue properties. The anti-fatigue properties of APPs correlated with their anti-oxidant activities and were exerted through eliminating free radicals and mediating an oxidative balance. The stronger anti-fatigue activity of ALAPP is probably related to its structural characteristics. Collectively, our study provides a fundamental basis for the development and use of APPs in medicine and functional foods.
研究领域:
食品科学
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生物化学
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产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2014
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IF 3.065
Xiangnan Li,Jiale Huang,Zidan Wang,Xinde Jiang,Wencheng Yu,Yanmei Zheng,Qingbiao Li,Ning He
The alkaline extraction and acid precipitation of longan seed phenolic compounds were investigated to develop a more practical process than currently exists to isolate these chemicals. By using an alkaline buffer as the extraction solvent, the yield of total phenols reached 46.86±0.43 mg/g after conventional solid–liquid extraction. Because of the benefit achieved from the preconcentration process, an acid precipitation efficiency of 65.29 ± 0.38% was obtained, and the separation yield of phenols reached 22.04 ± 1.95 mg/g. However, the purity of the phenolic extract was influenced by the presence in the precipitate of proteins and carbohydrates, which both moved together in all four precipitation fractions at different pH values. The FRAP assay and the assay of the radical-scavenging activity revealed the strong antioxidant activity possessed by the phenolic compounds in the precipitate extracts. This preparation process for phenolic compounds has obvious advantages, such as easy operation, safe products and low production cost, which indicated its prospective industrial potential.
研究领域:
食品科学
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植物化学
✓ 已验证
产品关联
DOI
高影响因子
BIOMASS & BIOENERGY
·
2024
·
IF 5.8
Feng Liu,Tiantian Qi,Quanyuan Wei,Jie Zhang,Yingxia Li,Yongshui Qu
This study aimed to produce high-performance dissolving pulps from corn stalks using a combination of cold caustic extraction (CCE) and deep eutectic solvent (DES) method. Results showed that CCE not only removed hemicelluloses but also altered the structure of cellulose, increasing its polymerization degree and influencing the Fock reactivity of the dissolving pulp (reduced to 10.55%). Subsequent treatment with DES effectively dissolving the remaining hemicelluloses while disrupting the cellulose crystallinity and improving the Fock reactivity of the dissolving pulp (increased to 72.14% from 10.55%). The study implied that the hydrogen bond donor in DES played a crucial role in breaking the cellulose chains and improving the reactivity of the dissolving pulp. The dissolving pulp produced from corn stalks using through the combination of CCE and DES method exhibited the cellulose content of 94.46% and the Fock reactivity of 72.14%, providing a new strategy for the high-value utilization of stover resources.
研究领域:
生物化学工程
·
可再生资源利用
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产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2020
·
IF 4.244
Qiuyan Chen,Xinping Wang,Hai Huang,Shilin Cao,Lihui Chen,Liulian Huang,Xiaojuan Ma
The development of effective separation of hemicelluloses from fully bleached cellulosic pulp fibers is conducive to the development and utilization of high value-added cellulose products. In this study, Turkey Red Oil (sulfonated castor oil) (TRO), a renewable source, was proposed to facilitate hemicelluloses separation from chemical pulp in cold caustic extraction (CCE). As expected, TRO application can significantly promote hemicelluloses separation in the CCE process. By contrast to the traditional CCE process, TRO/CCE could decrease the hemicelluloses content from 9.1 to 6.1%, while the hemicelluloses removal selectivity increased from 72.5 to 82.3% and efficiency, increased from 52.4 to 68.1%. Moreover, without sacrificing hemicelluloses removal, the TRO utilization can reduce the alkaline consumption and therefore preserve the cellulose I crystal from conversion to cellulose II. Additionally, the TRO/CCE procedure made the cellulose fiber more flexible and more accessible to react with CS 2 and therefore a high Fock reactivity.
研究领域:
制浆造纸工程
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可再生资源利用
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生物质化学
✓ 已验证
产品关联
DOI
高影响因子
Polymers
·
2024
·Vol. 16
·期 10
·1403
·
IF 4.7
Shuyu Pang,Xin Wang,Jiali Pu,Chen Liang,Shuangquan Yao,Chengrong Qin
Hemicellulose extracted by alkali treatment is of interest because of the advantages of its intact sugar structure and high degree of polymerization. However, the hemicellulose extracted by alkali treatment contained more lignin fragments and the presence of a lignin–carbohydrate complex (LCC), which affected the isolation and purification of hemicellulose and its comprehensive utilization. Therefore, the evaluation of the LCC structure of different types of lignocellulosic resources is of great significance. In this study, the LCC structures of hardwoods and Gramineae were enriched in alkaline systems. Information on the composition, structural proportions, and connection patterns of LCC samples was discussed. The similarities and differences between the LCC structures of different units of raw materials were comparatively studied. The results indicated that the monosaccharide fractions were higher in the LCC of Gramineae compared to hardwoods. The composition of the lignin fraction was dominated by G and S units. The phenyl glycosidic (PhGlc) bond is the predominant LCC linkage under alkali-stabilized conditions. In addition, Gramineae PhGlc types are more numerous compared to hardwoods. The results of the study provide insights into the differences in the chemical composition and structural features of LCC in different plants and provide important guidance for the optimization of the process of purifying hemicellulose.
研究领域:
植物学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Bioresources and Bioproducts
·
2025
·
IF 13
Xin Wang,Yang Liu,Shiyu Luo,Baojie Liu,Shuangquan Yao,Chengrong Qin,Shuangfei Wang,Chen Liang
Understanding the differences in the chemical structures of important components in different bamboo tissues is crucial for maximizing bamboo utilization and biorefining bamboo resources. Hemicellulose and lignin-carbohydrate complex (LCC) were extracted from bamboo green, bamboo core, and bamboo yellow tissues by using the alkali-leaching method, and the chemical composition, thermal stability, dissolution process, and structural characteristics were analyzed. The extraction yield of hemicelluloses followed the order: bamboo yellow > bamboo core > bamboo green. Hemicelluloses extracted from bamboo green mainly originated from the secondary wall (S-layer) of the fiber cells and parenchyma cell walls, while those from the bamboo core and yellow mainly originated from the inner S-layer and outer S-layer of the fiber cells, as well as the parenchyma cell walls. The LCCs from bamboo core and bamboo yellow contained a large number of type I phenyl glycoside (PhGlc 1 ) bonds, which mainly originated from the parenchyma cell walls of these tissues. These findings provide data on the structural differences between carbohydrate components in green, core, and yellow bamboo, offering valuable guidance for the high-value utilization of different bamboo tissues.
研究领域:
植物学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Haofang Wan,Saiya Zhou,Chang Li,Huifen Zhou,Haitong Wan,Jiehong Yang,Li Yu
Safflower seed extract has antioxidant, anti-inflammatory and other effects. The difference in antioxidant activity of safflower seed extracts obtained by different prediction models remains to be studied. In the present study, single factor screening of safflower seed was used to determine the level of optimization, and a four-factor and three-level optimization system was developed. The optimal extraction conditions were obtained by response surface methodology (RSM) and Ant colony algorithm-enabled back propagation neural network (ACO-BPNN) optimization, respectively. The two optimal conditions were verified and in vitro antioxidant studies were performed. Based on the single factor results, the optimization range was determined to be the ultrasonic frequency of 160–320 W, the ethanol concentration of 70–90 %, the liquid-to-material ratio of 15–25 mL/g, and the extraction temperature of 60–80 °C. Validation of both models and in vitro antioxidant results showed that ACO-BPNN results were superior to RSM. The prediction (actual) conditions of ACO-BONN were ultrasonic frequency of 303.4576 (320) W, ethanol concentration of 79.6549 (80)%, liquid-to-material ratio of 15.0027 (15) mL/g and extraction temperature of 82.2419 (80) °C. The predicted value of ACO-BPNN was 0.2042, the actual value was 0.1871 ± 0.0110, and the results of DPPH and FRAP were 2.28 ± 0.42 and 1.64 ± 0.12 mmol Trolox/g. In vivo antioxidant results showed that safflower seed extract could significantly increase the level of SOD in blood ( P < 0.01), reduce the level of MDA ( P < 0.05). Simultaneously, it significantly increased the expression of HO-1 ( P < 0.01), and inhibited the expression of NOX2 ( P < 0.05) in brain tissue. It provids experimental basis for clinical development of safflower seed. It provides reference for the large-scale extraction of safflower seed.
研究领域:
化学工程
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药学
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生物技术
✓ 已验证
产品关联
DOI
高影响因子
Food Bioscience
·
2023
·
IF 5.2
Fengyu Jiang,Yuanhao Sheng,Feilin Wang,Haihui Pan,Weiming Chen,Fansheng Kong
As a waste of sugarcane, the added value of sugarcane leaves has not been fully utilized, and sugarcane leaf polysaccharide is an important active component of sugarcane leaves. Using new extraction methods to study the structure and biological activity of polysaccharides from sugarcane leaves is of great significance for the development and application of sugarcane leaves. To determine the structural characteristics and biological activities of different acidic sugarcane leaf polysaccharides (ASLPs) extracted by microwave-assisted hot alkali extraction (MALE), three new acidic sugarcane leaf polysaccharides (ASLP-50, ASLP-70 and ASLP-90) were precipitated by ethanol. The structure of ASLPs extracted by MALE contains protein, sulfate and uronic acid . ASLP-50 (195.88 kDa), ASLP-70 (94.62 kDa) and ASLP-90 (16.37 kDa) were acidic polysaccharides composed of mannose , galacturonic acid, glucose, arabinose and fucose . The results of XRD and NMR showed that ASLPs were amorphous polysaccharides composed of α-L-Araf-(1→ main chain, and its branching points were composed of Arap and GalpA residues. In addition, ASLPs showed significant antioxidant, hypoglycemic, hypolipidemic and antibacterial activities in vitro . The research and application of ASLPs provide a reference for the recycling of sugarcane leaves, which is conducive to the development of food , agriculture and pharmaceutical industries.
研究领域:
农业科学
·
食品科学
·
药学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Fuhui Luo,Zhongqiao Zhao,Yong He,Fenfen Zeng,Xia Yang,Fengxiang Zhang,Wei Shi
Nelumbinis Plumula, a dual-use medicinal and edible plant, has been processed into a functional drinks and exhibits pharmaceutical potential via bioactive compounds. Here, a new solvent system composed of alkaline electrolyzed water (AEW) and deep eutectic solvents (DES) (AEWDES) has been developed. Screening 30 solvents revealed AEWDESs (L-proline-1-methylurea) (LP-JJN) outperformed ultrasound assisted DESs and traditional solvents in extraction efficiency. We propose a novel hybrid framework integrating Crested porcupine optimizer-Backpropagation neural networks-Sparrow search algorithm (CPO-BP-SSA) for alkaloids extraction optimization. The yield enhancement primarily stems from AEWDES-mediated cell wall disruption increasing compound accessibility and augmented intermolecular interactions (hydrogen bonding, Van Der Waals force) between target alkaloids and DES components. Green metrics validated the method's sustainability. AEWDES extraction method is universal and can serve as a reference for the extraction of various alkaloids. Future research should prioritize the recovery of alkaloids compounds through advanced extraction and desorption techniques.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMEDICAL CHROMATOGRAPHY
·
2021
·Vol. 36
·期 3
·e5293
·
IF 1.902
Yongjing Liu,Qiang Chen,Suxia Zhang,Hua Zhang,Wei Xu
Deep eutectic solvents (DESs) were applied as eco-friendly solvents in this study for the extraction of alkaloids from lotus leaf, including O- nornuciferine, N- nornuciferine, nuciferine and roemerine. A series of hydrophilic and hydrophobic DESs with different hydrogen bond donors and a acceptors were synthesized and screened for a suitable DESs for extraction of alkaloids from lotus leaf. The study results showed that the hydrophilic DES with choline chloride and propanediol had the highest extraction yield. The main factors affecting the extraction efficiency—choline chloride–propanediol ratio, water content in deep eutectic solvents, solid–liquid ratio and extraction time—were investigated via a single-factor experiment. The optimized extraction conditions were 30% of water in choline chloride–propanediol (1:4) for heated extraction for 30 min and solid–liquid ratio 1:100 g/ml. Under optimum conditions, the extraction yields of O- nornuciferine, N- nornuciferine, nuciferine and roemerine were 0.069, 0.152, 0.334 and 0.041 g/100 g respectively, which were higher than those of methanol in acidified aqueous solution. This study suggests considerable potential for DESs as promising materials for the green and efficient extraction solvents for bioactive alkaloids from natural sources.
研究领域:
化学工程
·
药学
·
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2022
·Vol. 28
·期 1
·46
·
IF 4.927
Huaizhi Pan,Biao Zheng,Hui Yang,Yingying Guan,Liuyang Zhang,Xiaoli Xu,Aimin Wu,Huiling Li
Hemicellulose is the second most abundant natural polysaccharide and a promising feedstock for biomaterial synthesis. In the present study, the hemicellulose of loblolly pine was obtained by the alkali extraction-graded ethanol precipitation technique, and the hemicellulose-polyvinyl alcohol (hemicellulose-PVA) composite film was prepared by film casting from water. Results showed that hemicellulose with a low degree of substitution is prone to self-aggregation during film formation, while hemicellulose with high branching has better compatibility with PVA and is easier to form a homogeneous composite film. In addition, the higher molecular weight of hemicellulose facilitates the preparation of hemicellulose-PVA composite film with better mechanical properties. More residual lignin in hemicellulose results in the better UV shielding ability of the composite film. This study provides essential support for the efficient and rational utilization of hemicellulose.Keywords:loblolly pine;hemicellulose;graded ethanol precipitation;hemicellulose-polyvinyl alcohol composite film
研究领域:
材料科学
·
生物材料
·
林业
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）