ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Shanshan Sun,Shujuan Wang,Shanshan Li,Liping Wei,Xiaohan Wang,Yizhou Wang,Bing Li,Yang Hu,Liangsheng Wang
Phytosterols exhibit a wide range of pharmacological activities and have no side-effects, which have attracted more and more attention. In this study, two pre-treatment methods (alkaline hydrolysis and acid-alkali hydrolysis) were performed in parallel for the first time to investigate the effects on phytosterol analysis. The aim is to develop an analytical method for phytosterols in pollen and anther wall of tree peony ( Paeonia ostii ‘Fengdan’) by gas chromatography-mass spectrometry. The result indicated that the contents of phytosterols in pollen (3390.02 mg/100 g DW) and anther wall (929.70 mg/100 g DW) of P. ostii ‘Fengdan’ were relatively high. Meanwhile, eleven phytosterols were identified, among which five phytosterols were first identified in tree peony pollen. Moreover, the effects of wall breaking and different developmental stages on phytosterol contents of pollen and anther wall of P. ostii ‘Fengdan’ were also discussed. The result showed that wall breaking was beneficial to sterol detection of pollen, but not necessary for anther wall. For the best harvest time, the stage of S2 had the highest content of phytosterols. In conclusion, this study successfully establishes an analytical method for phytosterols by gas chromatography-mass spectrometry, and lays a foundation for the development and utilization of pollen and anther wall of P. ostii ‘Fengdan’ in functional foods and pharmaceutical industry .
研究领域:
植物学
·
药理学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2023
·Vol. 12
·期 14
·2718
·
IF 5.2
Qinzhang Jiang,Shengwei Wang,Yuzhe Yang,Jinxin Luo,Ruili Yang,Wu Li
Rice bean (Vigna umbellata) is a medicinal and dietary legume rich in polyphenols. In this study, the free and bound phenolics in rice bean were extracted by water, 80% methanol, and acid, base, and composite enzymatic hydrolysis, respectively. The polyphenol profiles of the extracted fractions were analyzed. The outcome demonstrated that base hydrolysis was the most effective way to liberate bound phenolics from rice bean (14.18 mg GAE/g DW), which was 16.68 and 56.72 folds higher than those extracted by acid and enzymatic hydrolysis, respectively. The bound polyphenols released by base hydrolysis contributed to 71.15% of the total phenolic content. A total of 35 individual phenolics was identified, of which isoquercitrin, procyanidin B1, rutin, taxifolin, and catechin were the main monomeric phenolics in the free fraction, while gallic acid, protocatechuic acid,p-hydroxybenzoic acid, catechin, and phloroglucinol were the main monomeric phenolics in the bound fraction. In comparison to the free phenolics extracted by water and 80% methanol and the bound phenolics extracted using acid and composite enzymatic hydrolysis, the bound phenolics from base hydrolysis had a superior antioxidant capacity. The antioxidant activity of rice bean is primarily attributed to individual phenolics such as catechin, abundant both in free and bound fractions, and alsop-hydroxybenzoic acid, gallic acid, and protocatechuic acid in bound fractions. The bound phenolics of rice bean were first reported and showed large differences with the composition of free phenolics. This work suggests that the bound fraction of rice bean must be taken into account in assessing its potential benefits to health.Keywords:rice bean;free phenolics;bound phenolics;antioxidant
研究领域:
食品科学
·
营养学
·
植物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Ruimiao Chang,Huichun Wang,Shumian Liu,Yijing Dong,Guangbin Zhang,Anjia Chen,Ruiping Zhang,Yong Li
For rapid separation of quercetin from apple peel, a novel magnetic nanomaterial was prepared by a simple and economical thermal alkaline hydrolysis method in this study. This magnetic nanomaterial was obtained by mixing and then heating magUiO-66-NH 2 (magnetic University of Oslo 66-NH 2 ) with 0.05 mol·L −1 KOH aqueous solution, and was named magUiO-66-NH 2 -MH 0.05 (magUiO-66-NH 2 -mixed heating 0.05 ), which has better magnetic properties, thermal stability, pore capacity and average pore size than magUiO-66-NH 2 . The obtained magUiO-66-NH 2 -MH 0.05 had strong adsorption capacity for quercetin, and the maximum adsorption capacity was 65.30 mg·g −1 . MagUiO-66-NH 2 -MH 0.05 tends to be monolayer-layer adsorption for quercetin, and electron sharing or transfer may occur during the adsorption process. Compared with naringenin, naringin and gallic acid, magUiO-66-NH 2 -MH 0.05 showed good recognition ability for quercetin, and the recovery rate was good. After 7 cycles, the removal rate of magUiO-66-NH 2 -MH 0.05 for quercetin was 96.3 % of the initial adsorption capacity. A magnetic solid-phase extraction method coupled with High-Performance Liquid Chromatography based on magUiO-66-NH 2 -MH 0.05 was successfully used for rapid separation and determination of quercetin in apple peel. This study will help to discover the experimental law of the treatment of UiO-66-NH 2 nanomaterials by hot alkaline hydrolysis, reveal the reaction mechanism, and expand the application of UiO-66-NH 2 nanomaterials in food analysis.
研究领域:
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Foods
·
2020
·Vol. 9
·期 12
·1816
·
IF 4.092
Liuying Zhu,Wenting Li,Zeyuan Deng,Hongyan Li,Bing Zhang
The composition and antioxidant activity of bound phenolics in three legumes (soybean, vicia faba, and kidney bean), and their metabolism and bioaccessibility in the gastrointestinal tract were investigated in this study. The total phenolic content, total flavonoid content, and antioxidant activities (ABTS and FRAP) were evaluated. The phytochemical compositions of the three legumes after acid/alkaline hydrolysis, simulated gastrointestinal digestion, and colonic fermentation were identified and quantified by UPLC-ESI-QTOF-MS/MS and HPLC-ESI-QqQ-MS/MS. The results showed that the three legumes were rich in bound phenolic compounds, and possessed a strong antioxidant activity; among which kidney bean showed a higher bound flavonoid content and antioxidant activity than the other two legumes. Alkaline hydrolysis allowed a more thorough extraction of the bound phenolics of the three legumes than acid hydrolysis. The released contents of bound phenolics were extremely low in in vitro digestion, whereas colonic fermentation favored the release of more phenolic compounds. Kidney bean, which presented the highest bound flavonoid content and antioxidant activity, had the lowest bioaccessibility. Our study provides a wider insight into the constituents and bioavailability of bound phenolic compounds in the three legumes.Keywords:soybean;vicia faba;kidney bean;acid/alkaline hydrolysis;in vitro digestion;colonic fermentation
研究领域:
食品科学
·
植物化学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Rui-Miao Chang,Yan-Yang Zhang,Guang-Bin Zhang,Xiao-Xia Zhang,An-Jia Chen,Wen Zhang,Yong Li
The purpose of this study was to treat UiO-66 (University of Oslo 66) under suitable thermal alkaline hydrolysis condition to realize the loading of gallic acid. UiO-66-SH (UiO-66-separated-heating) was obtained by separated heating UiO-66 and 0.2 M KOH aqueous solution to 120 ℃ before mixing for 3 h. The material was in an amorphous state, maintained the octahedron structure and size of UiO-66. UiO-66-SH has better porosity and specific surface area than UiO-66, and had good thermal stability until heated to 1000 ℃. Furthermore, UiO-66-SH had very little influence of the cellular activity of human normal heptical cell line, demonstrating its good biocompatibility. The prepared UiO-66-SH could successfully adsorb gallic acid and control the release of gallic acid in simulated gastric fluid (∼58% vs. ∼ 88% of free gallic acid). This study will be conducive to preparation of appropriate carrier used to load with polyphenolic compounds such as gallic acid.
研究领域:
材料科学
·
药物递送
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2018
·Vol. 23
·期 2
·270
·
IF 3.098
Chunlan Tang,Bingchu Wu,Jinyi Wu,Zheng Zhang,Bocheng Yu
Gastrodia elataBlume (G. elata), a traditional Chinese medicine, is widely used for treatment of various neuro dysfunctions. However, its quality control is still limited to the determination of gastrodin. In the present study, two novel strategies based on quantitative evaluation of total gastrodin and gastrodigenin with base hydrolysis and total gastrodigenin with base-enzymatic hydrolysis followed by HPLC-FLD were put forward and successfully applied to evaluate the quality of 47 batches ofG. elatafrom eight localities. Meanwhile, a systematic comparison of the novel strategy with the multiple markers and the Pharmacopeia method was performed. The results showed that the parishins category could be completely hydrolyzed to gastrodin by sodium hydroxide solution, and gastrodin could further utterly hydrolyze to gastrodigenin with β-d-glucosidase buffer solution. The contents of total gastrodin and gastrodigenin ranged from 1.311% to 2.034%, and total gastrodigenin from 0.748% to 1.120% at the eight localities. From the comparison, we can conclude that the two novel strategies can comprehensively reveal the characteristics of overall active ingredients inG. elatafor quality control. The present study provides a feasible and credible strategy for the quality control ofG. elata, suggesting a revision of the latest Chinese Pharmacopoeia or European Pharmacopoeia methods for the modernization ofG. elatause.Keywords:total gastrodin;total gastrodigenin;base-enzymatic hydrolysis;HPLC-FLD
研究领域:
药理学
·
分析化学
·
中药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2021
·
IF 8.742
Yafei Li,Xinda You,Runlai Li,Ya Li,Chao Yang,Mengying Long,Runnan Zhang,Yanlei Su,Zhongyi Jiang
Preparation of membranes with both ultrathin thickness and loose structure is often required for high separation performance, but remains a grand challenge. Herein, we report a two-step method to acquire ultrathin loose polyamide (ULPA) nanofilms. More specifically, in the first step, we prepare the ultrathin polyamide nanofilms by support-free interfacial polymerization and in the second step we loosen the ultrathin polyamide nanofilms by alkali hydrolysis. The uniform distribution and diffusion of piperazine at the aqueous-organic interface accelerates the self-sealing and self-healing of interfacial polymerization and generates ultrathin but dense polyamide nanofilms. Further, by hydrolyzing amide bonds with alkali solution, the pore size of polyamide nanofilms is dramatically enlarged. The composite membranes with ULPA nanofilm as separating layer display a water permeance of 29.3 L m −2 h −1 bar −1 , 160% higher than that of the pristine polyamide membrane, with an ultrahigh Na 2 SO 4 rejection of 99.1% at 1000 ppm concentration and an outstanding Cl − /SO 4 2− selectivity of 91.8. This ultrathin loose structure may provide a new solution to design membranes with extraordinary permselectivity.
研究领域:
材料科学
·
环境工程
✓ 已验证
产品关联
DOI
高影响因子
Autex Research Journal
·
2024
·Vol. 24
·期 1
·
IF 1.1
Sihui Pan,Hongfei Qian
In this study, ultrasound energy was applied to assist the alkali hydrolysis of polyethylene terephthalate (PET) fabric, and then, the results were compared with those of the mechanical oscillating method, which was used as the control. The effects of various factors such as the sodium hydroxide concentration, the dosage of dodecyl dimethyl benzyl ammonium chloride (DDBAC), and the frequency on the weight loss of the PET fabrics were systematically investigated. The surface appearance and microstructures of the treated fibers with different methods were analyzed using a scanning electron microscope and X-ray diffraction, respectively. The results showed that DDBAC played a prominent accelerating role in the hydrolysis of the PET polymer, and the frequency had a great influence on the weight loss of the PET fabric. Ultrasound with a frequency of 60 kHz showed a similar decomposition rate as the control, resulting in similar weight loss, which was the highest value among the three frequencies (20, 60, and 80 kHz). In addition, the application of ultrasonic energy led to more pits on the fiber surface, a smaller average grain size, and decreased crystallinity of the treated fibers, while the mechanical oscillating method resulted in slightly increased crystallinity. By comparing the K/S value of the dyed fabrics with two commercial disperse dyes, we found that the treatment method had no obvious correlation with the color depth of the treated fabric.
研究领域:
材料科学
·
纺织工程
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Xiabin Chen,Yun Zhang,Junsen Tong,Pengfei Ouyang,Xingyu Deng,Jie Zhang,Huan Liu,Yihui Hu,Weixuan Yao,Jiye Wang,Xia Wang,Shurong Hou,Jianzhuang Yao
Enzyme therapy for cocaine detoxification should break down both cocaine and its primary toxic metabolite, benzoylecgonine (BZE), which is also the main form of cocaine contaminant in the environment. An ideal BZE-metabolizing enzyme (BZEase) is expected to be highly efficient and selective in BZE hydrolysis. Here, BZEase4 was engineered from bacterial cocaine esterase (CocE) by our reactant state-based enzyme design theories (RED), which has a 34,977-fold improved substrate discrimination between BZE and the neurotransmitter acetylcholine (ACh), compared with wild-type CocE. Under the physiological concentrations of BZE and ACh, the reaction velocity of BZEase4 against BZE is 2.25 × 10 6 -fold higher than it against ACh, suggesting BZEase4 has extremely high substrate selectivity for BZE over ACh to minimize the potential cholinergic side-effects. This study provides additional evidence supporting the further development of BZEase4 toward a promising therapeutic for cocaine overdose, a potentially effective and eco-friendly enzymatic method for BZE degradation in the environment.
研究领域:
生物化学
·
药物开发
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD PROPERTIES
·
2018
·
IF 1.845
Xue Li,Jun Cao,Xinpeng Bai,Fangfang Zhang
Fish oil is beneficial to health and its quality is significantly impacted by the extraction method. To determine the effect of various methods used to extract the oil on its chemical composition and thermal properties, tilapia oil extracted by dilute alkali hydrolysis, enzymatic hydrolysis, and microwave-assisted methods were investigated in this study. Nutrient analyses showed that freeze-dried tilapia contained fat (36.69 g/100 g freeze-dried tilapia), protein (49.95 g/100 g freeze-dried tilapia), ash (12.04 g/100 g freeze-dried tilapia) and moisture (1.21 g/100 g freeze-dried tilapia). Chemical analysis showed that the fish oil extracted by dilute alkali hydrolysis had the lowest acid value (AV) and peroxide value (POV) (AV: 0.77 mg KOH/g oil and POV: 3.76 mmol/kg oil) compared to the oil extracted using the other methods. The main fatty acid components were palmitic acid (22.78–23.16%), oleic acid (25.73–26.19%), and linoleic acid (15.69–15.98%). Furthermore, the oil extracted by dilute alkali hydrolysis showed significantly higher content of DHA+ EPA (2.30%). The thermal behaviors indicated that the aforementioned oils had significantly different crystal structures. The main endothermic peaks of fish oil extracted by dilute alkali hydrolysis, enzymatic hydrolysis and microwave-assisted methods were located at −2.03℃, −3.20℃ and −4.37℃, respectively. However, infrared spectroscopic analyses indicated them had the same characteristic functional groups. Consequently, different extraction methods significantly affect the oil’s physical and chemical properties without affecting spectral characteristics. Furthermore, these results advocated the use of alkali hydrolysis for fish oil extraction.
研究领域:
食品科学
·
化学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
CELLULOSE
·
2024
·Vol. 31
·期 4
·2583-2601
·
IF 4.9
Wang Lei,Hu Hanchang,Du Yu,Mi Xiang,Zhu Qiuyu,Chen Qiulin,Gui Zuwen,Zhang Bin,Yu Zhicheng
Based on the trend of low-carbon emissions and environmentally friendly development, this paper extracted natural dye from gardenia for cellulose fiber dyeing and endowed them with UV resistance and antibacterial properties. And more attention was paid to the dye structure of the gardenia yellow dye and its alkali-hydrolyzed gardenia yellow dye on dye uptake, UV–visible absorption characteristic peak as well as functional finishing. The main dye components of gardenia yellow dye and its alkali-hydrolyzed gardenia yellow dye were measured by the UV–visible and LC–MS spectrum. The crocin, which was the main dye components in the gardenia yellow dye, presented higher maximum absorption wavelength and darker colors compared with alkali-hydrolyzed gardenia yellow dye, which the main dye components was croceic acid. The cotton fabrics dyed with gardenia yellow dye or alkali-hydrolyzed gardenia yellow dye all indicated well dyeing performacne and functional characteristics such as antioxidant activity, UPF values and antibacterial property against Staphylococcus aureus and Escherichia coli . The main reason for different in the dyeing and functional performance of cotton fabrics dyed with gardenia yellow dye or its alkali-hydrolyzed dye were the removing gentiobiose molecules under alkali treatment, which changed the conjugate systems, ionization capacity, and polyphenols and flavonoids content. And the study examines the dyeing kinetics, adsorption isotherm, and thermodynamics of gardenia yellow dye and its alkali-hydrolyzed gardenia yellow dye on the cationic cotton. It is expected that achievement of this research provide guide to the value adding utilization of agricultural by-products from crop industries and view to investigate the effect of dye composition on the dyeing and functional performance. Graphical
研究领域:
纺织化学
·
天然染料
·
可持续纺织品
·
功能整理
·
植物提取
·
环境科学
·
染整技术
✓ 已验证
DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Xitong Liu,Fengzhang Wang,Jirong Wang,Ting Liu,Chengying Zhao,Jinkai Zheng
Pectin and flavonoids are important components of citrus fruits, but the effects of flavonoid grafting on the emulsifying properties of citrus pectin remain unclear. In this study, a novel natural citrus flavonoid-pectin conjugate in citrus peel was identified through alkaline hydrolysis combined with ultra-high performance liquid chromatography. Subsequently, two major citrus flavonoids were enzymatically conjugated to citrus pectin at high grafting rates (hesperidin: 20.21%; naringin dihydrochalcone: 16.11%). The flavonoids increased pectin amphipathy while ensuring a tighter conformation and denser network structure. These structural changes reduced the interfacial tension (from 20.6 to 18.4 mN/m) and increased the adsorbed layer thickness (from 11.8 to 37.8 nm), facilitating rapid pectin anchoring at the oil-water interface. These led to smaller droplet size and better emulsion stability. Prepared flavonoid-pectin conjugates with high grafting rates exhibited better emulsifying properties than those with medium grafting rates; hesperidin-pectin conjugates were more effective than naringin dihydrochalcone-pectin conjugates. The prepared flavonoid-pectin conjugates aid in the structural identification of natural flavonoid-pectin conjugates; they also facilitate analyses of the properties of these conjugates and offer new insights into improving the functional properties of pectins used in the food industry.
研究领域:
食品科学
·
化学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）