INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Luyao Bian,Jingchao Fu,Tingting Chang,Chuang Zhang,Chong Zhang
In response to the growing demand for biodegraded film with high mechanical properties for complex preservation application scenarios, we developed a curdlan (CD) blended films with exceptional mechanical strength through an alkali dissolution method. Notably, the alkali-soluble CD film exhibited five-fold increase in tensile strength (TS) when compared to its water-soluble counterpart. Furthermore, the inclusion of 2 % bacterial cellulose (BC) resulted in a significant 41.1 % augmentation of the film's TS. Thermal stability improvements were observed through differential scanning calorimetry (DSC) analysis and thermogravimetric analysis (TGA). X-ray diffraction (XRD) and Fourier-transform infrared spectroscopy (FTIR) results provided insights into alterations in film crystallinity and intermolecular interactions. Specifically, the incorporation of 10 % CEO led to an additional improvement in TS. Our experimental investigations involving the packaging of chilled fresh meat with these blended films unveiled their capacity to effectively inhibit microorganism growth, maintain meat color stability, delay protein decomposition and fat oxidation , and extend the storage time up to 9 days. Our study offers a promising solution for food packaging, emphasizing the development of a high-strength degradable CD/BC/CEO blended film, which holds potential for extending the shelf life of food products.
研究领域:
食品科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
Gels
·
2025
·Vol. 11
·期 6
·387
·
IF 5.3
Zhixing Li,Chenglei Sun,Fan Wang,Zhaofei Xia
Alkali-soluble polysaccharides fromPoria cocos(APCP) are typically discarded due to poor water solubility and limited bioavailability, despite their β-(1→3)-glucan backbone suggesting potential for functional applications. This study aimed to explore the structural characteristics, gelation behavior, and the capacity of APCP to reduce silver ions. Structural analysis confirmed that APCP is a homogenous β-(1→3)-D-glucan with a molecular weight of 314.2 kDa and a PDI of 1.32. A pH-mediated strategy enabled the formation of stable single-component APCP hydrogel (APCPH) with tunable mechanical strength, high swelling capacity (>590%), and thermal stability. The APCPH further acted as both a reducing and stabilizing matrix for in situ AgNP formation. Notably, the Ag-APCP hydrogel exhibited distinct antibacterial activity, with inhibition zones reaching 5.31 mm againstStaphylococcus pseudintermedius. These findings demonstrate the feasibility of transforming underutilized APCP into multifunctional hydrogel platforms for green nanomaterial synthesis and biomedical applications. Future studies will focus on optimizing AgNP synthesis parameters and evaluating long-term stability and biocompatibility for translational use in antimicrobial therapies.
研究领域:
生物材料学
·
纳米医学
·
药用高分子材料
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抗菌治疗
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绿色合成
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PROGRESS IN ORGANIC COATINGS
·
2015
·
IF 2.358
Lu Shen,Yinwen Li,Jian Zheng,Mangeng Lu,Kun Wu
A series of UV-curable oligomers based on bisphenol-F epoxy resin were synthesized via end-capping of acrylic acid (AA) and 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS) in different ratios. Along with the increase of AMPS content, viscosities of modified resins reduced obviously. Integrated performance of cured films was also evaluated. Water swelling and contact angle tests indicated that the hydrophilicity was enhanced by the incorporation of sulfonate and amide groups into the coatings. Thermal degradation behaviors showed that the char residue, as well the glass transition temperature of the cured films was increased with the rising AMPS proportion, though the initial decomposition temperature declined. Moreover, doping of plentiful AMPS caused a remarkable enhancement in elongation at break, which was ascribed to greater flexibility of polymer chains and reduced density of crosslinking structure. Cured films with AMPS modified showed strong adhesive strength resulted from strong hydrogen bonding formed between amide sulfonate groups from AMPS and polar surface of glass substrates. For pure AA modified film, it was unaffected in NaOH solution (2 wt%) for 24 h. While for AMPS modified film, the film could be completely removed in several minutes when exposed to alkali solution. The modified epoxy films with these attractive features had prospective applications in temporary protective coatings, which could resist acid and organic solvents while would be easily wiped off from the substrate by soaking in the alkali solution.
研究领域:
材料科学
·
化学工程
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产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2018
·
IF 3.849
Dong Huang,Mengliu Zhang,Wei Chen,Dawei Zhang,Xinluan Wang,Huijuan Cao,Qian Zhang,Chunyan Yan
Cibotium barometz is a tropical and subtropical plant, mainly distributed in the Malaysian Peninsula and southern China. In China, C. barometz has tremendous commercial value in horticultural and medicinal applications. In the present study, alkaline soluble crude polysaccharides (CBBs) were extracted from water extraction residues of C. barometz with sodium hydroxide solution. After deproteinization and dialysis, CBBs were isolated and purified via DEAE-52 cellulose and Sephadex G-75 gel filtration chromatography to obtain two polysaccharide fractions (CBBP-2 and CBBP-3), with relative molecular weights of 10.7 kDa and 1.23 × 10 2 kDa, respectively. Chemical methods and nuclear magnetic resonance (NMR) studies indicated that CBBP-2 was composed of →4)- α - d -Glc p -(1→, →3)- α - l - R ha p -(1→, →3)- α - l -Ara f -(1→, →6)- β - d -Gal p -(1→, →3,6)- α - d -Man p -(1→ and α - d -GalA p -(1→, and CBBP-3 was composed of →6)- β - d -Gal p -(1→, →3)- α - l - R ha p -(1→, →3)- α - l -Fuc p -(1→, →3)- α - l -Ara f -(1→, →3,6)- α - d -Man p -(1→, β - d -GalA p -(1→ and α - d -Gal p -(1→. Morphological and conformational analyses indicated that CBBP-2 had a fragmented and sawdust-like structure. CBBP-3 had a neuron-like morphology, with a number of dendritic branches. Neither CBBP-2 nor CBBP-3 had a triple helix structure. Furthermore, MC3T3-E1 cells were selected to evaluate the osteogenic activities of CBBP-2 and CBBP-3 in an in vitro anti-osteoporosis assay, and the results revealed that CBBP-2 significantly promoted the differentiation of MC3T3-E1 cells at concentrations of 9.4 μM and 18.8 μM and promoted osteogenic mineralization at a low concentration at 4.7 μM. Overall, the systematic extraction, purification, structure identification and bioactivity assays of CBBP-2 and CBBP-3 showed that CBBP-2 could potentially be used as an anti-osteoporotic activity ingredient, with applications in health care and pharmaceutical industries.
研究领域:
药理学
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生物化学
·
分子生物学
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产品关联
DOI
高影响因子
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
·
2020
·Vol. 246
·期 7
·1483-1494
·
IF 2.366
Zhu Jiangxiong,Du Mengke,Wu Meirong,Yue Pengxiang,Yang Xiufang,Wei Xinlin,Wang Yuanfeng
In this study, two novel mixed ACE-inhibiting peptides, namely Se-TAPep 1 and TAPep 1, were prepared and purified from selenium-enriched and common alkali-soluble proteins using a series of process, and then its physicochemical properties and oligopeptide sequence were analyzed. The results showed that Se-TAPep 1 and TAPep 1 could be successfully separated, but their ACE inhibitory activities were different (IC50 values were 13.39 mg/mL and 23.56 mg/mL, respectively), this may be related to the difference in selenium content. Further analysis of physicochemical properties showed that the protein or peptide fractions contained their characteristic absorption peaks and contained aromatic amino acids, while the apparent morphology was different, the presence of selenium could change the apparent morphology of protein or polypeptide fractions, arranging of selenium-enriched fractions more irregular. The amino acid composition analysis showed that the Pro content of the selenium-enriched fractions was higher than that of the ordinary component, whereas the oligopeptide sequence analysis showed that both LQPSLGFP derived from Se-TAPep 1 and AETGEIKGHY derived from TAPep 1 contained some characteristics of highly active ACE-inhibiting peptides, but the characteristics of LQPSLGFP were more conspicuous, which may be related to its molecular weight and source of mixed peptides. These findings indicated that the selenium-enriched tea mixed peptides had better potential uses than ordinary tea mixed peptides in the preparation of functional food.
研究领域:
食品科学
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生物化学
·
药理学
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产品关联
DOI
高影响因子
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
·
2020
·Vol. 58
·期 2
·532-540
·
IF 1.946
Xing Li,Miao Yelian,Li Na,Jiang Ling,Chen Jie Yu
One water-soluble polysaccharide (WDOP) and three alkali-soluble polysaccharides (ADOP1, ADOP2 and ADOP3) were successfully obtained from Dendrobium officinale . Molecular structure of the polysaccharides was analyzed, and in vitro lactic acid fermentation of the polysaccharides by lactic acid bacteria (LAB) and bifidobacteria was performed. All the polysaccharides exerted proliferative effect on the LAB and bifidobacteria. ADOP2 was the most effective one, followed by WDOP. This could be attributed to their molecular structure features, such as a high level of total sugar, uronic acid and reducing sugar, an abundance of glucose and mannose, and a low and middle weight-average molecular weight. ADOP2 and WDOP produced more short-chain fatty acids (SCFAs) than ADOP1 and ADOP3 did during lactic acid fermentation. Propionic and acetic acids were the main SCFAs produced. These findings are useful for understanding the structure–activity relationship of D. officinale polysaccharides in lactic acid fermentation, and for developing new functional foods and beverages from D. officinale .
研究领域:
食品科学
·
微生物学
·
植物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Wan Li,Kexin Fang,Hao Yuan,Dongru Li,Haochen Li,Yin Chen,Xinyao Luo,Lian Zhang,Xiaochuan Ye
Poria cocos alkali-soluble polysaccharide (PCAP), a water-insoluble β-glucan, is the main component of the total dried sclerotia of Poria cocos . However, its gelation behaviour and properties have yet to be comprehensively studied. In this study, an acid-induced physical hydrogel based on natural PCAP is fabricated. The acid-induced gelation in PCAP is explored with respect to the pH and polysaccharide concentration. PCAP hydrogels are formed in the pH range of 0.3–10.5, and the lowest gelation concentration is 0.4 wt%. Furthermore, dynamic rheological, fluorescence, and cyclic voltammetry measurements are performed to elucidate the gelation mechanism. The results reveal that hydrogen bonds and hydrophobic interactions play a dominant role in gel formation. Subsequently, the properties of the PCAP hydrogels are investigated using rheological measurements, scanning electron microscopy, gravimetric analysis, free radical scavenging, MTT assays, and enzyme-linked immunosorbent assays. The PCAP hydrogels exhibit a porous network structure and cytocompatibility, in addition to good viscoelastic, thixotropic, water-holding, swelling, antioxidant, and anti-inflammatory activities. Furthermore, using rhein as a model drug for encapsulation, it is demonstrated that its cumulative release behaviour from the PCAP hydrogel is pH dependent. These results indicate the potential of PCAP hydrogels for application in biological medicine and drug delivery.
研究领域:
材料科学
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药学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2022
·
IF 8.943
Yan Li,Yutan Chu,Hongyu Sun,Qiongli Bao,Yizong Huang
Arsenic (As) is a common environmental pollutant that seriously interferes with the normal growth of organisms. There is an urgent need to take environment-safe and efficient strategies to mitigate As toxicity. Melatonin (MT) is a pleiotropic molecule that regulates plant growth and organ development and alleviates heavy metal stresses. The experiment aims to explore the mechanism of MT in reducing arsenite toxicity by hydroponic rice seedlings. The results showed that MT application reduced the As content in rice roots and shoots by 26.4% and 37.5%, respectively, and mainly decreased As content in the soluble fractions of the rice root cell. MT application also increased the As content of chelated-soluble pectin and alkali-soluble pectin in the cell wall by 14.7% and 74.4%, respectively. It promoted the generation of the functional group of the root cell walls by the FTIR analysis, indicating that MT may promote the fixation of As on the cell wall. Meanwhile, MT contributed to scavenging excess H 2 O 2 , reducing MDA content, and maintaining normal morphology of root cells by stimulating SOD, POD and CAT activities and increasing the level of GSH. The research deepens our understanding of how MT participates in maintaining redox homeostasis in rice cells, reducing As toxicity, and decreasing As concentration in rice seedlings, thereby providing more possibilities for reducing As accumulation in rice.
研究领域:
植物生理学
·
环境毒理学
·
农学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2017
·
IF 4.529
Ting Hu,Qilin Huang,Kahing Wong,Hong Yang,Jingsi Gan,Yanru Li
An alkali-soluble polysaccharide was extracted from Lignosus rhinocerotis sclerotia (LRP). Its structural characteristics were determined by GC–MS, FT-IR, GC, 1D and 2D NMR combined with Smith degradation and methylation analysis. The LRP had a (1 → 3)-β- d -Glcp backbone with every three residues bearing a (1 → 6)-linked and hyperbranched side chain that contained three (1 → 6)-β- d -Glcp residues as secondary main chain and two terminal β- d -Glcp residues linked at O3. The degree of branching was 0.76 from GC–MS analysis, implying a highly branched structure for LRP. The M w , < S 2 > z 1/2 , R h and [ η ] values of LRP in 0.25 M LiCl/DMSO were measured by SEC-MALLS-Vis-RI combination technology to be 2.88 × 10 5 g/mol, 30.36 nm, 22.34 nm and 131.50 ml/g, respectively. Furthermore, the exponent α of [ η ] –M w , β of < S 2 > z 1/2 –M w , the fractal dimension d f and molecular parameter ρ were determined to be 0.20, 0.33, 2.50 and 1.36, demonstrating that the LRP was a hyperbranched polysaccharide and adopted a compact coil conformation in LiCl/DMSO.
研究领域:
生物化学
·
多糖化学
·
药学化学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）