NANO
·
2016
·
IF 0.951
Huixiang Wu,Changjun Hou,Huanbao Fa,Liang Dong,Yi Ma,Mei Yang,Caihong Shen,Jun Zhou,Danqun Huo
Cartap is among the most popular pesticides that are widely used to boost food production, however, its residues pose a great threat to human health and the environment. We presented simple and effective colorimetric sensor based on aggregation of gold nanoparticles (AuNPs) with appropriate diameter and fluorescent sensor via inner filter effect (IFE) of AuNPs on cadmium telluride quantum dots (CdTe QDs), respectively. Firstly, effect of diameter of AuNPs was investigated and the results indicated that AuNPs with diameter of 13 nm (AuNPs 1 3 nm ) showed better sensitivity toward cartap. Thus, AuNPs 1 3 nm was chosen for colorimetric and fluorescent cartap assay. On one hand, mercaptoacetic acid (MA) was functionalized on AuNPs 1 3 nm for the improvement of anti-disturbance ability for discrimination study. A decent linear relationship for cartap was obtained in the range from 1 μ M to 50 μ M with detection limit of 1.32 μ M for colorimetric readout; on the other hand, fluorescent sensor based on AuNPs 1 3 nm displayed excellent linear relationship with cartap concentration varying from 20 nM to 100 nM with low detection limit of 4.02 nM. And the above-mentioned sensor showed acceptable anti-disturbance ability for cartap discrimination. These results demonstrated that the sensor as-developed would be a great potential candidate for cartap detection in real application.
研究领域:
分析化学
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环境科学
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纳米技术
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DOI
高影响因子
JOURNAL OF FOOD SCIENCE
·
2014
·Vol. 79
·期 12
·C2434
·
IF 1.791
Qi Wang,Beibei Wang,Meihu Ma,Zhaoxia Cai
en This link goes to a English section pt This link goes to a English section Sialic acids (SA) are widely found as components of oligosaccharide units in mucins, glycoproteins and other microbial polymers in nature food. The aim of this study is to create a new fluorimetric detection method applied for determinating SA in egg products by using a sensitive lectin-CdTe quantum dots (QDs) nanoprobe. Water-soluble and high luminescent CdTe QDs were conjugated with sambucus nigra bark lectin (SNA) as probe for SA detection. As a result of specific interaction between SA and SNA-CdTe QDs, the conjugations finally lead to the change of a fluorescent signal. Under optimal conditions, fluorescence intensity increase linearly with the increase of the concentration of SA ranging from 12 to 680 ng/mL. The low detection limit is 0.67 ng/mL. This quick and selective analysis method for SA detection has been used in synthetic samples and egg products with recovery between 97.92% and 110.42%, which demonstrates the application of this assay is feasible and practical. Practical Application en This link goes to a English section pt This link goes to a English section Sialic acids (SA) is an important component in nature food. We developed a new fluorimetric method to determinate SA by using a sensitive lectin-CdTe quantum dots nanoprobe. This method for quick and selective SA detection has been used in egg products.
研究领域:
分析化学
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食品科学
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纳米技术
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Yueying Wang,Wenting Li,Xuetao Hu,Xinai Zhang,Xiaowei Huang,Zhihua Li,Miaoyun Li,Xiaobo Zou,Jiyong Shi
A ratiometric fluorescence sensor system is proposed for detecting bis(2-ethylhexyl) phthalate (DEHP) in pork, which is based on aptamer recognition with molybdenum disulfide quantum dots and cadmium telluride quantum dots (MoS 2 QDs/CdTe-Apta). Two signals exist in the system, among which the response signal is transmitted by CdTe-Apta. The amide condensation between aptamers and CdTe QDs shortens the distance between CdTe QDs and DEHP, thus quenching the fluorescence of CdTe QDs, possibly through a photoinduced electron transfer mechanism. The MoS 2 QDs deliver the self-calibration signal, and the fluorescence of MoS 2 QDs remains almost constant when co-existing with DEHP. Linearity (R 2 = 0.9536) was established for the DEHP concentration range 0.005–3.0 mg·L −1 , with a limit of detection of 0.21 μg·L −1 . The system was successfully applied in the determination of DEHP in pork. The system has potential for the quantitative determination of DEHP in practical applications.
研究领域:
分析化学
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环境科学
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食品科学
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DOI
PubMed
高影响因子
ANALYTICAL LETTERS
·
2024
·
IF 1.6
Xixuan Gu,Qingning Li,Xiaohui Song,Chen Chen,Xiaobing Yin,Jiayu Shen,Rui Yu,Keqi Tang
The concentration of alpha-fetoprotein (AFP) generally increases in the serum of hepatocellular carcinoma (HCC) patients. Hence, AFP is a biomarker for HCC detection, diagnosis, and treatment. However, the sensitivity is unsatisfactory. Here a fluorescent probe based on CdTe quantum dots (QDs-AFPAb) was prepared and a simple sensitive fluorescence immunoassay on nitrocellulose membranes (NFIA) was developed for alpha-fetoprotein. This method exhibited a low detection limit of 2.18 pg/mL with a linear range from 10 to 1.0 × 107 pg/mL. In addition, the sensitivity allowed the identification of HCC and non-HCC serum. This method is sensitive, easy, and low-cost for the diagnosis of HCC.
研究领域:
生物技术
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分析化学
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医学诊断
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癌症研究
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DOI
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 3
·1-7
·
IF 5.479
Wang Jiaxing,Zhang Linlin,Lu Liping,Kang Tianfang
An electrochemiluminescence (ECL) based biosensor is described for determination of microRNAs in the A549 cell line. Firstly, graphene oxide (GO) is dripped onto a glassy carbon electrode surface to form an interface to which one end of the capture probe (with a stem-loop structure) can be anchored through π-interaction via dangling unpaired bases. The other end of the capture probe is directed away from the GO surface to make it stand upright. Target microRNAs can open the hairpin structure to form a double-stranded DNA-RNA structure. Two auxiliary probes, generating a hybridization chain reaction, are used to elongate the DNA duplex. Finally, doxorubicin-modified cadmium telluride quantum dot nanoparticles (Dox-CdTe QD) are intercalated into the base pairs of the hybrid duplexes to act as signalling molecules. The ECL signal of the Dox-CdTe QD increases proportionally with the concentration of microRNAs, specifically for microRNA-21. The assay covers a wide linear range (1 fM to 0.1 nM), has a low detection limit for microRNA-21 (1 fM), and is selective, reproducible, and stable.
An enzyme-free amplification electrochemiluminescent assay is described to quantitative detection of microRNA in the A549 cell line. Graphene oxide was used to immobilize capture probes obviating the special modification. Doxorubicin-modified cadmium telluride quantum dot nanoparticles are intercalated into the base pairs of the hybrid duplexes to act as signalling molecules.
研究领域:
生物化学
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纳米技术
·
分析化学
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生物医学工程
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DOI
PubMed
高影响因子
ARCHIVES OF TOXICOLOGY
·
2018
·Vol. 92
·期 4
·1421-1434
·
IF 5.728
Jiang Shengwei,Lin Yuchun,Yao Huan,Yang Chuanli,Zhang Liyin,Luo Bing,Lei Zhao,Cao Liwei,Lin Naibo,Liu Xiangyang,Lin Zhongning,He Chengyong
Unfolded protein response (UPR) and endoplasmic reticulum (ER)-phagy are essential for cell homeostasis. Quantum dots (QDs), which have been widely used for biomedical applications, can accumulate in the kidney tissues and may cause renal dysfunction. However, the molecular mechanism of QDs-induced nephrotoxicity is still obscure. The present study was aimed to elucidate the role and mechanism of UPR and ER-phagy in QDs-induced nephrotoxicity. Herein, human embyronic kidney (HEK) cells were exposed to 15, 30, 45, and 60 nM cadmium telluride (CdTe)-QDs for 12 and 24 h. And CdTe-QDs (30–60 nM) inhibited the HEK cell viability. The clathrin-dependent endocytosis was determined as the main pathway of CdTe-QDs cellular uptake. Within cells, CdTe-QDs disrupted ER ultrastructure and induced UPR and FAM134B-dependent ER-phagy. Blocking UPR with inhibitors or siRNA rescued the FAM134B-dependent ER-phagy, which was triggered by CdTe-QDs. Moreover, suppression of UPR or FAM134B-dependent ER-phagy restored the cell vability. In vivo, mice were intravenously injected with 8 and 16 nmol/kg body weight CdTe-QDs for 24 h. Kidney was shown as one of highest distributed organs of CdTe-QDs, resulting in renal dysfunction, as well as UPR and FAM134B-dependent ER-phagy in it. Thus, for the first time, we demonstrated that ER-phagy can be triggered by nanomaterials both in vitro and in vivo. In addition, blocking of UPR and ER-phagy showed protective effects against CdTe-QDs-induced toxicity in kideny cells. Notably, a secreted alkaline phosphatase reporter gene system has been developed as a sensitive and rapid method for evaluating the ER quality under the exposure of nanomaterials.
研究领域:
毒理学
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细胞生物学
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纳米技术
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药理学
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DOI
PubMed
高影响因子
ANALYTICAL LETTERS
·
2023
·
IF 2
Jiajia Wang,Xiaomin Yang,Xin Hua,Yanan Li,Baokang Jin
Previous studies confirm that aberrant expression of cytokeratin 19 fragment antigen 21-1 (cyfra-21-1) is highly correlated with non-small cell lung cancer (NSCLC). Herein, a proportional electrochemical immunosensor based upon a methylene blue/cadmium telluride/molybdic sulfide (MB/CdTe/MoS2) nanocomposite was successfully fabricated for detecting tumor marker cyfra-21-1. The result indicates that MB/CdTe/MoS2 modified on the surface of a glassy carbon electrode (GCE) supplies a large surface area for immobilizing primary antibodies (Ab1), ensuring high selectivity and sensitivity. To further amplify the sensitivity of the biosensor, ferrocenecarboxylic acid (Fc-COOH) linked with amino-functionalized secondary antibodies through amido bond (Ab2-Fc) as the “signal-on” probe and MB-modified with MoS2 with a large specific surface area as the “signal-off” probe increase the number of active sites for integration of Ab2-Fc/Ag/BSA/Ab1/MB/CdTe/MoS2/GCE electrode with the linker cyfra-21-1 (Ag). Meanwhile, CdTe accelerates the electron transfer and enhances the MB current, improving the sensitivity and detection limit. The presence of cyfra-21-1 led to both the decreasing response of MB and increasing current responses of Fc upon the electrode. Based upon this strategy, the determination of cyfra-21-1 was achieved in a linear range from 10 pg/mL to 10,000 ng/mL with the lowest detectable concentration of 10 pg/mL (signal-to-noise ratio of 3). The electrochemical immunosensor performs a sensitive determination of cyfra-21-1, possibly providing a practical strategy for the clinical analysis of lung cancer.
研究领域:
生物化学
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分析化学
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肿瘤学
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纳米技术
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电化学
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DOI
高影响因子
Science of Advanced Materials
·
2021
·Vol. 13
·期 5
·864-873
·
IF 1.474
Chen Ping,Yue Xinai,Dai Wei,Wang Xia,Li Yingying,Xie Hongjian,Jiang Dongmei,Jiang Yongmei
N-acetyl-L-cysteine-modified CdTe QDs (Cadmium Telluride Quantum Dots) were obtained by hydrothermal method, and DA (amino deoxyglucose), PEG (polyethylene glycol), and 9-p-D (9 polyamine acid) were obtained by ligand substitution, which were used for remodifying CdTe
QDs. Successively, a novel CdTe QDs nanoma-terial, CdTe-NALC, was obtained. This composite nanometer material was characterized by ultraviolet, fluorescence spectrum, nuclear magnetic resonance hydrogen-spectrum, and other methods to study further its biocompatibility and toxic effect on the
primary cultured mouse hippocampus neurons. PC12 cells were vitro-cultured and dissolved in the CdTe-NALC solution with different concentrations. After 24 h, the expression levels of proteins related to the cAMP (cyclic adenosine monophosphate)-CREB (cAMP response element-binding proteins)-BDNF
(brain-derived neurotrophic factor) signaling pathway in the cells were detected by ELISA (enzyme-linked immunosorbent assay) kit. In the experiment, the DA/9-p-D/PEG-modified CdTe QDs composite nanometer material was successfully manufactured by ligand replacement. The nanoparticles
had good dis-persibility with an average particle size of about 9.2 nm. DA/9-p-D/PEG modification improved the biocompatibil-ity of the QDs. CdTe composite nanomaterials could significantly reduce the cell activity of mouse hippocampal neurons and promote their apoptosis, with an evident
dose-apoptosis relationship. With the increase of CDTE-NALC solution concentration, cAMP, pCREB (phosphorylated CREB), and BDNF protein content decreased (P < 0.05).
研究领域:
纳米技术
·
神经科学
·
细胞生物学
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生物化学
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Wenchao Geng,Linsheng Xue,Yuling Li,Jiangying Ji,Xinxin Yuan,Lihua Ding,Ruiying Yang
Herein, a novel silver ion-loaded gold microemulsion assemblies (Au/Ag + MAs) mediated multifunctional signal amplification strategy was proposed to construct a sensitive immobilization-free photoelectrochemical (PEC)/colorimetric biosensor for carcinoembryonic antigen (CEA) detection. Through the sandwiched reaction among CEA, the CEA aptamer (DNA1) loaded on the Au nanoparticles (NPs) functionalized iron oxide (Fe 3 O 4 ) nanospheres and another CEA aptamer (DNA2) immobilized on Au/Ag + MAs , a complex is formed and acquired by magnetic separation. Then, Au/Ag + MAs of the complex are disassembled into Au NPs and Ag + ions driven by an acetone response, and the obtained demulsification solution is transferred to the cadmium sulfide/cadmium telluride (CdS/CdTe) photoactive composites modified electrode . Based on the multiple inhibition functions (blocking effect of oleylamine; energy transfer effect of Au NPs; and electron snatching effect of Ag + ), the photocurrent of the electrode decreases obviously, resulting in the ultrasensitive detection of CEA (a detection limit of 16 fg mL −1 ). Interestingly, the ion-exchange reactions between CdS/CdTe composites and Ag + ions generate silver sulfide/silver telluride (Ag 2 S/Ag 2 Te) composites, and a color change of composites can be distinguished directly, leading to a quick visual detection of CEA. Compared with the traditional single-modal assay for CEA, such dual-modal PEC/colorimetric assay is a more accurate and reliable due to different mechanisms and independent signal conversion. This work will offer a new perspective for the applications of various self-assemblies in PEC bioanalysis .
研究领域:
生物化学
·
分析化学
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纳米技术
·
生物传感器
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 12
·1-9
·
IF 5.833
Tang Zhuoyun,Wei Zeliang,Huang Ke,Wei Yinhao,Li Dongdong,Yan Shixin,Huang Jin,Geng Jia,Tao Chuanmin,Chen Piaopiao,Ying Binwu
Human immunodeficiency virus (HIV) infection inflicts significant economic and social burdens on many countries worldwide. Given the substantial morbidity and mortality from HIV infection, there is an urgent need for accurate and early detection of the virus. In this study, immunofluorescence and visual techniques are described that detect the HIV-1 p24 antigen, which relied on selective recognition of Ag + /Ag nanoparticles (Ag NPs) and Cu 2+ /Cu + using cadmium telluride quantum dots (CdTe QDs). After the sandwich immunoreactions were accomplished, the alkaline phosphatase (ALP) hydrolyzed L-ascorbic acid 2-phosphate (AAP) to form ascorbic acid (AA) that further reduces Ag + and Cu 2+ to Ag NPs and Cu + , respectively. This method was highly sensitive and selective and could detect as low as 1 pg/mL of p24 antigen by naked eyes and had a good linearity in the concentration range 1–100 pg/mL. When using Ag + and Cu 2+ as media, the limit of detection (LOD) of the new method was 0.3 pg/mL and 0.2 pg/mL, respectively. Compared with clinical electrochemiluminescence immunoassay (ECLIA) results and clinical data, this method demonstrated good consistency for the quantification of HIV-1 p24 antigen in 34 clinical serum samples. In addition, this method could accurately distinguish HIV from other viruses and infections such as hepatitis B virus , systemic lupus erythematosus , hepatitis C virus , Epstein-Barr virus , cytomegalovirus , lipemia , and hemolysis . Therefore, our dual-mode analysis method may provide additional solutions to identify clinical HIV infection. Graphical abstract An immunofluorescence and visualization dual-mode strategy for the detection of p24 antigen was constructed based on immune recognition reaction and a phenomenon that cadmium telluride quantum dots (CdTe QDs) can selectively recognize Ag + /Ag nanoparticles (Ag NPs) and Cu 2+ /Cu + .
研究领域:
病毒学
·
免疫学
·
医学诊断
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产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2026
·
IF 5.2
Xinyang Zhang,Yanning Qu,Beibei Lou,Ru Yan,Dongfeng Sun,Yuan Yu
Interfacial photocatalysis has been developed; however, in liquid–liquid heterogeneous systems, photocatalytic efficiency remains limited by stability and mass transfer constraints. To address the challenges, we constructed a Pickering emulsion (PE) system stabilized by Janus-structured amphiphilic TiO2 nanoparticles (J-TiO2) at oil–water interfaces. The hydrophilic and hydrophobic groups on J-TiO2 were, respectively, extended into the water phase and oil phase (n-octanol) to stabilize the heterogeneous system for effective interfacial catalysis. The resulting J-TiO2-stabilized PEs exhibited a long-term stability (>35 days) and an enhanced hydrogen (H2) evolution rate of 185.6 μmol/g/h, with a total H2 production yield of 535 μmol/g, representing a 2.98-fold increase over the single aqueous phase and an 11.9-fold enhancement relative to the pure oil phase. Combining experimental analysis and molecular dynamics (MD) simulations, we demonstrated that the amphiphilic J-TiO2 effectively assembled at the oil–water interface, significantly reducing the interfacial tension and facilitating charge separation for the redox reaction. This work provides a new strategy for designing liquid–liquid heterogeneous photocatalytic PEs systems and modifying their interfacial structures for potential industrial applications.
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DOI
PubMed
高影响因子
Journal of Materials Chemistry A
·
2020
·Vol. 8
·期 9
·4931-4937
·
IF 11.301
Zhiwei Huang,Dongyang Wang,Caiyun Li,Jinfeng Wang,Guangtao Wang,Li-Dong Zhao
In this work, the thermoelectric performance of p-type PbSe was improved dramatically by enhancing the Seebeck coefficient and reducing electronic thermal conductivity through CdTe alloying. CdTe alloying effectively increased the effective mass of p-type Pb0.98Na0.02Se due to band flattening and promoting the valence band convergence, which contributed to a high Seebeck coefficient of ∼238 μV K−1 at 860 K and large power factor of ∼18.2 μW cm−1 K−2 at 860 K for Pb0.98Na0.02Se + 4% CdTe. Furthermore, CdTe alloying reduced the carrier mobility of Pb0.98Na0.02Se due to the enhanced scattering of impurity atoms, which results in a remarkable reduction of electronic thermal conductivity. As a result, due to its higher power factor and lower thermal conductivity, a high thermoelectric figure of merit ZT ∼1.5 at 860 K was achieved for Pb0.98Na0.02Se + 4% CdTe. This paper provides one novel approach to enhance the thermoelectric performance of p-type PbSe through synergistically manipulating electrical and thermal transport properties.
研究领域:
材料科学
·
物理学
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DOI
高影响因子
危险品化学品经营许可证（带存储）