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Phthalate esters decreased nutritional value of rice grains via redirecting glycolytic carbon flow from grain quality formation toward antioxidative defense

Yingying Sun,Jie Chen,Lizhong Zhu

The prevalence and persistence of phthalate esters (PAEs) in agricultural soils has garnered global attention. Assessing their potential impacts on crop yield and quality necessitates a thorough understanding of their risks. In this study, we elucidated the carbon flow-dependent mechanisms of the decreased grain quality upon exposure to PAEs through a soil-based rice cultivation experiment. Combining metabolomics and transcriptomics methods, our findings revealed that the glycolytic intermediates derived from sucrose breakdown preferentially flowed towards amino acid synthesis, rather than starch and fatty acid synthesis under exposure to dibutyl phthalate (DBP) or di(2-ethylhexyl) phthalate (DEHP). This redirection led to decreased levels of starch (by 14–23 %) and fatty acids (by 10–40 %) in the grains. Notably, the increased amino acids primarily served as antioxidants to mitigate DBP and DEHP stresses, rather than enhancing protein quality. Consequently, a reduction in protein levels by 5.7–38 % was observed. Moreover, our study pinpointed glucose-6-phosphate, a common precursor for amino acids, fatty acids, and starch synthesis, as the crucial branching node in glycolysis that redirected this carbon flow. This study offers a new perspective for evaluating the ecological risks associated with PAEs, paving the way for future research and interventions to mitigate their adverse effects on crops.