ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2018
·
IF 3.974
Fei Li,Lin Di,Yuxin Liu,Qiuyun Xiao,Xiaoyu Zhang,Fuying Ma,Hongbo Yu
Although ascomycetes occupy a vaster niche in soil than the well-studied basidiomycetes , they have received limited attention in studies related to bioremediation. In this study, the degradation of carbaryl by Xylaria sp. was studied in different culture conditions and its possible metabolic pathway was elucidated. In liquid culture, 99% of the added carbaryl was eliminated when cytochrome P450 (CYP450) was active, which was similar to the degradation rate of Pleurotus ostreatus , a fungus with strong bioremediation ability. Mn 2+ is beneficial to the degradation of carbaryl. Compared to the 72.17% degradation rate in sterile soil, 59.0% carbaryl was eliminated in non-sterile soil, which suggested that Xylaria sp. BNL1 can resist microorganismal infection. Furthermore, the intracellular fractions containing laccase, CYP450, and carbaryl esterase efficiently degraded carbaryl. The presence of carbaryl metabolites suggested that Xylaria sp. BNL1 initiated its attack on carbaryl via carbaryl esterase to release α-naphthol, which was further degraded to 1,4-naphthoquinone and benzoic acid by CYP450 and laccase. Thus, our study highlights the potential of using Xylaria sp. for bioremediation.
研究领域:
环境科学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
·
2022
·Vol. 88
·期 4
·
IF 5.005
Zhijian Ke,Qian Zhu,Siyuan Gao,Mingliang Zhang,Mingli Jiang,Yijun Ren,Yali Liu,Yidong Zhou,Jiguo Qiu,Qing Hong
Previously, a LysR family transcriptional regulator, McbG, that activates the mcbBCDEF gene cluster involved in the upstream pathway (from carbaryl to salicylate) of carbaryl degradation in Pseudomonas sp. strain XWY-1 was identified by us (Z. Ke, Y. Zhou, W. Jiang, M. Zhang, et al., Appl Environ Microbiol 87:e02970-20, 2021, https://doi.org/10.1128/AEM.02970-20). In this study, we identified McbH and McbN, which activate the mcbIJKLM cluster (responsible for the midstream pathway, from salicylate to gentisate) and the mcbOPQ cluster (responsible for the downstream pathway, from gentisate to pyruvate and fumarate), respectively. They both belong to the LysR family of transcriptional regulators. Gene disruption and complementation study reveal that McbH is essential for transcription of the mcbIJKLM cluster in response to salicylate and McbN is indispensable for the transcription of the mcbOPQ cluster in response to gentisate. The results of electrophoretic mobility shift assay (EMSA) and DNase I footprinting showed that McbH binds to the 52-bp motif in the mcbIJKLM promoter area and McbN binds to the 58-bp motif in the mcbOPQ promoter area. The key sequence of McbH binding to the mcbIJKLM promoter is a 13-bp motif that conforms to the typical characteristics of the LysR family. However, the 12-bp motif that is different from the typical characteristics of the LysR family regulator binding site sequence is identified as the key sequence for McbN to bind to the mcbOPQ promoter. This study revealed the regulatory mechanisms for the midstream and downstream pathways of carbaryl degradation in strain XWY-1 and further our knowledge of (and the size of) the LysR transcription regulator family.IMPORTANCE The enzyme-encoding genes involved in the complete degradation pathway of carbaryl in Pseudomonas sp. strain XWY-1 include mcbABCDEF, mcbIJKLM, and mcbOPQ. Previous studies demonstrated that the mcbA gene, responsible for hydrolysis of carbaryl to 1-naphthol, is constitutively expressed and that the transcription of mcbBCDEF was regulated by McbG. However, the transcription regulation mechanisms of mcbIJKLM and mcbOPQ have not been investigated yet. In this study, we identified two LysR-type transcriptional regulators, McbH and McbN, which activate the mcbIJKLM cluster (responsible for the degradation of salicylate to gentisate) and the mcbOPQ cluster (responsible for the degradation of gentisate to pyruvate and fumarate), respectively. The 13-bp motif is critical for McbH to bind to the promoter of mcbIJKLM, and 12-bp motif different from the typical characteristics of the LysR-type transcriptional regulator (LTTR) binding sequence affects the binding of McbN to the promoter. These findings help to expand the understanding of the regulatory mechanism of microbial degradation of carbaryl.
研究领域:
微生物学
·
分子生物学
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INORGANIC CHEMISTRY
·
2023
·
IF 4.6
Yanqin Hu,Ben Qin,Xiaoyan Li,Fan Yang,Chuan-Pin Lee,Meiying Xie,Liyan Xue
With the development of nuclear energy, the reprocessing of 99TcO4–/ReO4– has become a very difficult problem due to environmental issues such as high output, long life, and easy leakage. In this study, three extraction systems containing carbamic acid were introduced into the reprocessing of 99TcO4–/ReO4– for the first time. The results involving one of the three results show that N-[N,N-di(2-ethylhexyl) aminocarbonylmethyl] glycine (D2EHAG) has ultrahigh selectivity for removal to 99TcO4–/ReO4–. When the extreme concentration ratio of SO42– and Cl– to ReO4– of D2EHAG is 10,000:1, the distribution coefficient of ReO4– still reaches 12.73 and 2.67, respectively. Additionally, the most hydrophilic NO3–, when the extreme concentration ratio of NO3– and ReO4– is 1000:1, still has a distribution coefficient close to 2.33, which is more than the most reported MOF adsorption materials. Moreover, the reaction kinetics, stripping rate, and reuse rate were studied. After five cycles, the removal rate is still 98.12%, with a decrease of less than 0.7%. The system containing carbamic acid is a potential extraction removal system to remove 99TcO4–/ReO4– from nuclear radioactive wastewater.
研究领域:
环境科学
·
核化学
·
放射性废物管理
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产品关联
DOI
PubMed
高影响因子
Chemosensors
·
2025
·Vol. 13
·期 3
·107
·
IF 3.7
Haiyan Chang,Hailong Wu,Tong Wang,Xiaozhi Wang,Ruqin Yu
In this study, an excitation–emission–pH multi-way fluorescence technique coupled with a third-order calibration method based on an alternating quadrilinear decomposition (AQLD) algorithm was proposed for the simultaneous determination of thiabendazole (TBZ) and carbaryl (CAR) in apples. AQLD can be considered a “mathematical separation” technique that extracts the pure signal of the target analyte from complex mixed signals, thereby effectively addressing fluorescence peak overlap and unknown interference. The average spiked recoveries of the target analytes ranged from 98.4% to 101.9%, and the relative standard deviation was less than 5.6%. To evaluate the performance of the method, a number of parameters were calculated, including sensitivity (SEN), selectivity (SEL), limit of detection (LOD), limit of quantification (LOQ), and intra-day and inter-day precision. The results of the third-order calibration method were compared with those of the second-order calibration method (based on excitation–emission matrix fluorescence). These results showed that the former was superior. In short, the proposed strategy is simple, cost-effective, and anti-interference, providing a valuable reference for accurate quantification of TBZ and CAR in complex food matrices with uncalibrated interferences.
研究领域:
分析化学
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食品科学
✓ 已验证
产品关联
DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2020
·
IF 4.872
Zi-Jian Chen,Xi-Xia Liu,Zhi-Li Xiao,Hui-Jun Fu,Yu-Ping Huang,Shu-Yi Huang,Yu-Dong Shen,Fan He,Xing-Xing Yang,Bruce Hammock,Zhen-Lin Xu
1-naphthol (1-NAP) is the main metabolite of pesticide carbaryl and naphthalene , and is also a genotoxic and carcinogenic intermediate in the synthesis of organic compound, dyes, pigment and pharmaceutical industry . In this work, two novel haptens were designed and synthesized for developing a competitive indirect enzyme-linked immunosorbent assay (ciELISA) method for 1-NAP in urine samples. The assay showed a limit of detection of 2.21 ng/mL and working range from 4.02 ng/mL to 31.25 ng/mL for 1-NAP in optimized working buffer. The matrix effect of samples was eliminated via 15-fold dilution of optimized working buffer. Good average recoveries (102.4%–123.4%) with a coefficient of variation from 11.7% to 14.7% was obtained for spiked urine samples. Subsequent instrument verification test showed good correlation between the results of ciELISA and high-performance liquid chromatography . The developed ciELISA is a high-throughput tool to monitor 1-NAP in urine, which can provide technical support for the establishment of biological exposure level for the exposure to carbaryl, naphthalene and other related pollutants.
研究领域:
环境生物学
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毒理学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
AUSTRALIAN JOURNAL OF CHEMISTRY
·
2014
·Vol. 68
·期 5
·793-799
·
IF 1.644
Sui Wang,Shasha Lv,Wenwen Wu,Zhiyong Guo
Sensitive electrochemiluminescence (ECL) detection and solid phase microextraction (SPME) using a NH2–graphene–Nafion modified glassy carbon electrode was developed for carbaryl. The NH2–graphene was synthesised and characterised by Fourier transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction. The main parameters that affect the extraction efficiencies, such as the buffer and sample pH, and extraction time were investigated and optimised. The introduction of NH2–graphene into Nafion improves the conductivity of the film because of its electrical conductivity. The electrode enables the determination of carbaryl in the range from 5 × 10–4 to 10 μg mL–1, and the limit of detection was 2 × 10–4 μg mL–1 at a signal-to-noise ratio of 3. The ECL intensity retained 97 % of its initial response current after storage for 10 days, indicating a good storage stability of the sensor. The relative standard deviations (RSDs) of intra-assay and inter-assay were found to be 3.5 and 5.3 %, respectively, indicating an acceptable reproducibility. Furthermore, the ECL sensor was successfully applied to the selective and sensitive quantitative determination of carbaryl in river samples, the recoveries of carbaryl ranged from 99.0 to 108.0 %, and the RSDs were less than 5.0 %, which shows good reproducibility and high precision of analysis.
研究领域:
分析化学
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
CHEMOSPHERE
·
2023
·
IF 8.8
Lihong Luo,Cuiwen Jiang,Hu Li,Dejiao Ning,Shuibing Lao,Zhongdan Liang,Li Tang,Weiwei Chen,Yu Ya
In this study, a novel electrochemical aptasensor for carbofuran (CBF) detection is prepared by gold nanoparticles decorated hierarchical porous carbon (Au@HPC). The prepared carbon materials show a three-dimensional hierarchical structure with a large specific surface area and a highly developed porous structure. Aptamers loading significantly improves when gold nanoparticles are embedded into the hierarchical porous carbon skeleton. Besides, Au@HPC modified electrode exhibits a large electroactive area and excellent electrochemical conductivity, serving as a promising platform for highly sensitive and selective electrochemical detection of CBF. The developed CBF electrochemical aptasensor shows a wide linear from 1.0 to 100000 pg/L with a detection limit of 0.5 pg/L, demonstrating an extraordinary sensitivity compared to other sensors for CBF detection. Additionally, the designed aptasensor was used to monitor the CBF in vegetable samples, with a recovery range from 98.4% to 104.8%. The results coincide with the standard test method, revealing its practicability in the food safety analysis.
研究领域:
分析化学
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食品安全检测
·
纳米材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Toxics
·
2024
·Vol. 12
·期 9
·654
·
IF 3.9
Zihan Niu,Chao Chen,Qijun Ruan,Yingming Duan,Shuqin Liu,Da Chen
Studying the interaction between pesticide contamination in the plant system and the dissolved organic matter (DOM) composition is important to understand the impact of pesticides and plants on the ecological function of DOM. The present study investigated the effects of DOM on the bioaccumulation and biotransformation of carbamates in plants, carbamate exposure on DOM composition, and plant root secretion on the interaction between DOM and carbamates. The concentrations of carbamates and their metabolites in living cabbage plants were continuously tracked through an in vivo analytical method. The presence of DOM was found to reduce the highest bioconcentrations and shorten the time it took to reach the highest bioaccumulated amounts of isoprocarb and carbofuran in plants, while it showed no significant effect on the uptake behavior of carbaryl. DOM profiling results indicated that carbamate exposure substantially decreased the number and molecular diversity of DOM. Notably, plant root secretion alleviated carbamate-induced DOM molecular alterations by inducing a higher turnover rate of DOM compared to that in the uncontaminated group, highlighting the role of plants in mitigating the effects of exogenous pesticide exposure on DOM composition and maintaining DOM molecular homeostasis.
研究领域:
环境科学
·
植物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Hengye Chen,Ou Hu,Yao Fan,Lu Xu,Lei Zhang,Wei Lan,Ying Hu,Xingrui Xie,Lixia Ma,Yuanbin She,Haiyan Fu
The needing of rapid and sensitive detection method for pesticides is increasing, to facilitate its detection without complicated instruments. Herein, a novel paper-based senor was developed for the visual detection of three carbamate pesticides (metolcarb, carbofuran, and carbaryl) based on CdTe quantum dots (QDs) and nano zinc 5, 10, 15, 20-tetra(4-pyridyl)-21 H -23 H -porphine (nano ZnTPyP) with a “turn-off-on” mode. This fluorescence sensing model could be applied in the highly selective and sensitive detection of carbamate pesticides both by fluorescence spectrometry or paper-based sensors. Based on the extracted RGB color values of paper, the partial least squares regression (PLSR) was used to accurately quantify the concentrations of carbamate pesticides in different food matrices (apple, cabbage and tea water). This method featured in high speed, low price and high accuracy, and provided a new strategy for the detection of food safety.
研究领域:
食品安全检测
·
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2021
·
IF 6.707
Li Zhang,Xuerui Yang,Runsheng Zhou,Sen Lin,Lei Zhou
In this study, Ni/Co layered double hydroxide (Ni/Co-LDH) with uniform flake structure was synthesized successfully as an effective activator in the carbamazepine (CBZ) degradation by peroxymonosulfate (PMS). Our results indicated that Ni/Co-LDH possessed a high specific surface area (72.9942 m 2 /g) and exhibited great activation performance, in which 100% CBZ removal could be achieved within 300 s under neutral conditions with 5 mg/L Ni/Co-LDH and 0.3 mmol/L PMS. In addition, the crystal structure and morphology of the prepared Ni/Co-LDH kept unchanged during degradation cycles. X-ray photoelectron spectroscopy (XPS) analysis and free radical identification experiment speculated that the cycle of Ni 2+ /Ni 3+ and Co 2+ /Co 3+ on Ni/Co-LDH surface were responsible for efficient HO•/SO 4 •− generation from PMS. Based on identified products through high resolution mass spectrometry (HR-MS), it was proposed that the degradation pathway of CBZ was a heterozygous process involving deamidation, hydroxylation and electron transfer. The ECOSAR calculation results indicated the toxicity of some products was still at very toxic level, thus certain attention should be paid to the toxicity of intermediates in the process of treating pollutants in water.
研究领域:
环境科学
·
化学
✓ 已验证
产品关联
DOI
高影响因子
Materials Today Communications
·
2023
·
IF 3.8
Xuejuan Huang,Pengcheng Wang,Qingqing Jiang,Juncheng Hu
Constructing three-dimensional (3D) hierarchical structure is regarded as an effective strategy to alleviate the inherent drawbacks of the BiOCl bulk structure and realize the optimization of photocatalytic performance. Herein, a unique BiOCl double-shelled hollow microsphere structure with adjustable shell thickness is fabricated through a template-free one-pot solvothermal method for the first time. A plausible formation mechanism is proposed by exploring the morphological changes during the growth process. The as-obtained hollow microspheres photocatalyst exhibits excellent carbamazepine (CBZ) degradation efficiency (97.5%) under simulated solar irradiation, the corresponding apparent rate constant is about 4 times and 3 times than that of conventional BiOCl solid microspheres. The experimental and characterization results show that the existence of nanosheet subunits and porous structures shorten the diffusion length of charge carriers, which is conducive to the separation and transfer of photogenerated. Meanwhile, the construction of double-shelled hollow structure achieves higher light utilization. This work demonstrates the superior performance of catalysts with hierarchical hollow structures for pollutant degradation and provides new ideas to further optimize the photocatalytic performance of BiOCl-based materials.
研究领域:
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
CHEMOSPHERE
·
2016
·
IF 3.698
Yongzhen Wang,Songquan Wu,Xingru Yan,Tao Ma,Lu Shao,Yuyan Liu,Zhanhu Guo
A series of alkyl bicarbamates supramolecular organogelators were synthesized with different structures and lengths of alkyl chains. The driving forces for the self-assembly of small molecules, including the intermolecular H bonding, π-π stacking and van der Waals interactions, played an important role in the formation of different 3D network structures, i.e., fibers, ribbons, sheets, and prisms. And a probable formation process of the gel networks was proposed. Furthermore, the phase-selective gelling performances were investigated for oil removal from aqueous solution. Interestingly, the gelling properties were found to be affected by the length and structure of alkyl chains, while some gelators with intermediate alkyl chain lengths could effectively gel all the tested oils from water surface within 15 min, such as Russian crude oil, diesel , gasoline, soybean oil, peanut oil, olive oil, cyclohexane , hexane and ethyl acetate. Advantageously, fast gelation , high rate of oil removal (>95%) and excellent oil retention rate (close to 100%) were realized in the recovery of oil spills from water surface. This kind of supramolecular gelators demonstrates good potential applications in the delivery or removal of organic pollution from oil/water mixtures.
研究领域:
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）