The embolic microspheres containing magnetic nanoparticles and anti-tumor drugs have been proposed for transcatheter arterial chemoembolization (TACE). However, this technique still suffers the poor control of hyperthermia temperature and drug release behavior. Herein, the magnetic microspheres based on low Curie temperature superparamagnetic iron oxide nanoparticles are developed by emulsification cross-linking of gelatin, genipin, and sodium alginate. The magnetic microspheres can self-regulate the hyperthermia temperature at around 50°C, un-necessitating any temperature control facilities. The magnetic microspheres can load doxorubicin hydrochloride and the loaded drug can be released in a controllable way by using an alternating magnetic field. Cytocompatibility and hemolysis evaluations confirm the non-cytotoxicity and negligible hemolysis of magnetic microspheres. The embolization model on rabbit auricular artery demonstrates that the magnetic microspheres can occlude the targeted blood vessel and are visualized under CT/MR imaging. All these findings suggest that the prepared magnetic microspheres could be used as the embolic agent in TACE. Statement of significance The existing magnetic embolic microspheres suffer the poor control of hyperthermia temperature and drug release behavior in TACE. In this work, we developed the magnetic embolic microspheres based on superparamagnetic iron oxide nanoparticles with a low Curie temperature. Upon the application of alternating magnetic field, the embolic microspheres can self-regulate the hyperthermia temperature at around 50°C and the drug loaded in the microspheres can be released in a somewhat controllable manner. The embolic microspheres are also detectable to both CT and MR. These characteristics enable the developed microspheres to simultaneously realize self-regulating temperature hyperthermia, on-demand drug release, embolization, and CT/MR imaging.
Yi Tao,Surun Huang,Xianghui Gu,Weidong Li,Baochang Cai
Alkaline phosphatase conjugated magnetic microspheres were synthesized via amide reaction, and employed as an effective adsorbent in affinity selection of binding ligands followed by UHPLC-Q-TOF/MS analysis. The analytical validity of the developed approach was evaluated under optimized conditions and the following figures of merit were obtained: linearity, 0.01–0.5 g L −1 with good determination coefficients (R 2 = 0.9992); limits of detection (LODs), 0.003 g L −1 ; and limits of quantitation (LOQ), 0.01 g L −1 . The precision (RSD%) of the proposed affinity selection approach was studied based on intra-day (0.8%) and inter-day (1.3%) precisions. Finally, the adsorbent was successfully applied to identification of binding ligands in Li-Dan-Hua-Shi pills and good recoveries were obtained in the range from 96.9 to 99.4% (RSDs 1.6–3.0%).
Chaofan Zheng,Huaili Zheng,Chao Hu,Yili Wang,Yongjuan Wang,Chun Zhao,Wei Ding,Qiang Sun
Magnetic biosorbents with specific morphological and molecular structure (PMCCs) were designed for the removal of ciprofloxacin (CIP) from water. Radical polymerization method was applied to immobilize the designed polymer brushes onto core-shell shaped magnetic microspheres to fabricate PMCCs. PMCCs exhibited a maximum adsorption capacity of 527.93 mg·g −1 , which is much higher than reported adsorbents, owing to the complete stretch of polymer brushes and increased active sites as well as enhanced interaction. The investigation on the adsorption behavior of PMCCs for CIP manifested that CIP adsorption well fitted the Langmuir isotherm model and pseudo-second-order kinetic model. The calculated thermodynamic parameters suggested that CIP adsorption onto PMCCs was spontaneous and exothermic. Further recycling experiments showed a loss of less than 20% in the CIP adsorption capacity after five times, demonstrating the reusability of the as-designed biosorbents.
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