COLLOIDS AND SURFACES B-BIOINTERFACES
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2014
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IF 4.287
Zhi Shan,Youjun Jiang,Mengyu Guo,J. Craig Bennett,Xianghai Li,Hefeng Tian,Ken Oakes,Xu Zhang,Yi Zhou,Qianming Huang,Huaping Chen
Maximizing DNA loading on magnetic nanoparticles (MNPs) is crucial for their successful utilization in gene transfer, DNA isolation, and bio-analytical applications. This enhancement is typically achieved by altering particle size and surfaces as well as charge density and ionic strength. We demonstrate a novel route for promoting DNA loading on amino-modified silica-coated magnetic nanoparticles (ASMNPs) by prior condensation of elongated DNA to a compact globule before adsorption. The enhanced DNA-loading capacity, as demonstrated by a reduction in the number of ASMNPs needed to achieve complexation, was presumably due to the elimination of DNA wrapping around nanoparticles and substantially reduced electrostatic interactions of DNA with nanoparticles because the compacted DNA globule conformation decreases its exposed surface charge. The maximum loading capacity of ASMNPs for condensed DNA was 4.4 times greater than that for elongated coiled DNA, achieving the highest ever reported value of 385 μg mg −1 . Practical applications for plasmid DNA isolation from cleared lysate confirmed the reliability of the proposed method.
研究领域:
生物化学
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纳米生物学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Magnetochemistry
·
2023
·Vol. 9
·期 7
·181
·
IF 2.7
Xiaogang Yu,Renpeng Yang,Chengwei Wu,Bo Liu,Wei Zhang
Zn0.6Mg0.4CrxFe2−xO4(0 ≤ x ≤ 0.4) nanoparticles were synthesized using a hydrothermal technique. The obtained magnetic nanoparticles (MNPs) exhibited a spinel structure, where the lattice constant decreased with the Cr3+ion content. The doping of Cr3+ion (x = 0.1) increased the specific saturation magnetization to 46.4 emu/g but decreased to 20.0 emu/g with the further increase in the Cr3+ion content to x = 0.4. The decrement in Curie temperature was ascribed to the weakened super-exchange interaction between the metal ions located atA-sites andB-sites, which arose from the doping of the Cr3+ion. The T2-weighted images gradually darkened with the increase in Zn0.6Mg0.4Cr0.1Fe1.9O4nanoparticles concentration, suggesting that the nanoparticles can enhance the image contrast. Zn0.6Mg0.4CrxFe2−xO4(0 ≤ x ≤ 0.4) nanoparticles were able to heat the agar phantom to the hyperthermia temperature under the safe alternating magnetic field, which showed their potential in the magnetic induction hyperthermia.Keywords:magnetic nanoparticles;Curie temperature;hyperthermia
研究领域:
材料科学
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医学影像
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生物医学工程
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产品关联
DOI
高影响因子
Materials
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2021
·Vol. 14
·期 18
·5125
·
IF 3.623
Chenhua Deng,Linjie Hou,Caifeng Zhang
Recyclable, cheap, eco-friendly, and efficient adsorbent materials are very important for the removal of pollution. In this work, we report the design and implementation of ferrimagnetic-humic acid nanocomposites as superior magnetic adsorbent for heavy metals. Ferrimagnetic and ferrimagnetic-humic acid nanocomposite particles with different morphologies were prepared using the coprecipitation method and hydrothermal synthesis method, respectively. The results show that the morphology of the nanoparticles prepared by the coprecipitation method is more uniform and the size is smaller than that by the hydrothermal synthesis method. Adsorption experiments show that the ferrimagnetic-humic acid nanoparticles prepared by the coprecipitation method has high sorption capacity for cadmium, and the maximum adsorption capacity is about 763 μg/g. At the same time, magnetic technology can be used to realize the recycling of ferrimagnetic-humic acid adsorbents.Keywords:ferrimagnetic nanoparticle;heavy metal;magnetic;adsorption
研究领域:
环境科学
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材料科学
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DOI
PubMed
高影响因子
Bioresources and Bioprocessing
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2018
·Vol. 5
·期 1
·1-8
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IF 0
Gao Zhen,Yi Yunfei,Zhao Jia,Xia Yongyang,Jiang Min,Cao Fei,Zhou Hua,Wei Ping,Jia Honghua,Yong Xiaoyu
Background Laccase, a multicopper oxidase that catalyzes the oxidation of phenols, aromatic amines, and benzenethiols, has attracted much attention in applications of organic synthesis, bioremediation, and pulp/textile bleaching. However, free laccases cannot be recycled and are easily inactivated in diverse environmental conditions. Enzyme immobilization is a promising strategy to improve stability, resistance to extreme conditions, and reusability of laccase. Results In this study, amino-functionalized magnetic Fe 3 O 4 nanoparticles were synthesized for co-immobilization of 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) and laccase by glutaraldehyde cross-linking method. The magnetic nanoparticles were characterized with FTIR, XRD and VSM. Cyclic voltammetry was carried out to verify electrochemical behaviors of the co-immobilized laccase and TEMPO nanoparticles. When the co-immobilized laccase and TEMPO nanoparticles were used to decolorize acid fuchsin, the maximum decolorization rate of 77.41% was obtained with the ratio of TEMPO to laccase being 0.3 mM/g:120 U/g. Conclusion The co-immobilized nanoparticles retained above 50% residual activity after eight cycles of operation, which presented an approach to develop a co-immobilized laccase and mediator system for potential industrial application.
研究领域:
生物化学
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环境科学
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DOI
MOLECULES
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2017
·Vol. 22
·期 2
·221
·
IF 2.861
Jing Chen,Juan Leng,Xiai Yang,Liping Liao,Liangliang Liu,Aiping Xiao
In this study, magnetic graphene oxide (MGO) nanomaterials were synthesized based on covalent binding of amino Fe3O4nanoparticles onto the graphene oxide (GO), and the prepared MGO was successfully applied as support for the immobilization of laccase. The MGO-laccase was characterized by transmission electron microscopy (TEM) and a vibrating sample magnetometer (VSM). Compared with free laccase, the MGO-laccase exhibited better pH and thermal stabilities. The optimum pH and temperature were confirmed as pH 3.0 and 35 °C. Moreover, the MGO-laccase exhibited sufficient magnetic response and satisfied reusability after being retained by magnetic separation. The MGO-laccase maintained 59.8% activity after ten uses. MGO-laccase were finally utilized in the decolorization of dye solutions and the decolorization rate of crystal violet (CV), malachite green (MG), and brilliant green (BG) reached 94.7% of CV, 95.6% of MG, and 91.4% of BG respectively. The experimental results indicated the MGO-laccase nanomaterials had a good catalysis ability to decolorize dyes in aqueous solution. Compared with the free enzyme, the employment of MGO as enzyme immobilization support could efficiently enhance the availability and facilitate the application of laccase.Keywords:dyes;graphene oxide;laccase;magnetic nanoparticles
研究领域:
环境科学
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生物技术
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2019
·
IF 8.355
Yuqi Geng,Jiaqi Li,Wenjuan Lu,Ning Wang,Zeyang Xiang,Yanzhao Yang
In this paper, superparamagnetic Fe 3 O 4 @SiO 2 -NH 2 nanoparticles were applied to adsorb Au(III)/Pd(II)/Pt(IV) in HCl medium. The successful synthesis of Fe 3 O 4 @SiO 2 -NH 2 was characterized through TEM, SEM and FT-IR. Electrostatic interaction between magnetic NPs and metal anions was proposed as the main adsorption mechanism by zeta potential analysis. Maximum adsorption capacities and adsorption behaviors under metal anions concentrations, acidity, NaCl, kinetic and isotherm models were investigated. Notably, the Fe 3 O 4 @SiO 2 -NH 2 shows the excellent acidity and salinity resistance on Au(III), Pd(II) adsorption. The separation routine among Au(III), Pd(II) and Pt(IV) was proposed. As investigated, Pt(IV) could be isolated due to the less adsorption amount onto Fe 3 O 4 @SiO 2 -NH 2 (≤1%). Then, the adsorbed Au(III) was eluted selectively by HCl + N-hexanol alcohol + ethanol + H 2 O 2 mixing solution. Then, the adsorbed Pd(II) was completely eluted by thiourea solution. The recovery efficiencies of Au(III), Pd(II) and Pt(IV) were kept over 92%, 97% and 94% respectively, during 5 turns of cycling separation experiments. Total loss amount of Fe is around 7%. Hence that, the particle demonstrates the good stability and high adsorption capacity, which possess great potential for practical Au(III), Pd(II) and Pt(IV) separation.
研究领域:
化学工程
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材料科学
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环境科学
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产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2017
·
IF 2.714
Zhi Zhang,Yueping Guan,Tingting Xia,Jiao Du,Tao Li,Zongying Sun,Chen Guo
To a certain extent, uneven coating of nanoparticles may lead to exposure of their contents. In this work, a simulation system was established and exposed Fe 3 O 4 magnetic nanoparticles (MNPs) were used to investigate the influence of the exposed case in a chemiluminescence immunoassay. Some factors such as the concentration of MNPs, coexistence of horseradish peroxidase (HRP), different particle sizes, and various treatment methods were investigated. It was confirmed that both the bodies of nanoparticles and metallic oxides on the exposed surface have associations with the detection signal. This conclusion may provide a new way to explain the deviation emerged in a chemiluminescence immunoassay. In addition, an exposed Fe 3 O 4 MNPs based chemiluminescence immunoassay was developed to detect human chorionic gonadotropin (hCG). Carboxyl-functionalized MNPs were modified with anti-α-hCG antibody, and further used as solid-phase supports for sandwich-type immunoassay. The detection limit for hCG under optimal conditions was as low as 1.184 ng/mL with a linear response range of 2–100 ng/mL and a correlation coefficient of 0.989. The proposed method could be used for the determination of hCG in serum samples, which demonstrated their application potential in rapid and cost-effective detection of biological samples.
研究领域:
纳米技术
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分析化学
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生物化学
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产品关联
DOI
高影响因子
Desalination and Water Treatment
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2015
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IF 1.173
Rong Ping Chen,Yin Long Zhang,Xiao Yan Wang,Cheng Yue Zhu,Ai Jun Ma,Wei Min Jiang
In this paper, magnetic Fe3O4/HA nanoparticles (MHA) were synthesized using a coprecipitation method. The structure and properties of the MHA were characterized by Fourier transform infrared spectroscopy, TG, transmission electron microscope, zeta potential, X-ray diffraction, and vibration sample magnetometer. MHA is proven to be stable in water and can be easily separated from solution under an external magnetic field. Adsorptive removal of methylene blue (MB) from aqueous solution using MHA was systematically studied. The fundamental adsorption experiments reveals that MHA is more efficient in dye removal when initial solution pH is higher than 4.0. The theoretical maximal adsorption capacity, which is based on the simulation results from the isothermal equilibrium study, is 200 mg/g. The adsorption kinetic and equilibrium behaviors of MHA are in agreement with pseudo-second-order model and Langmuir model, respectively. The value of activation energy (Ea) indicates that the adsorption of MB onto MHA is a physical process. The Gibbs free energy of the adsorption process (△G) is negative, indicating that the adsorption of MB onto MHA is thermodynamically favorable. Furthermore, MHA could be easily regenerated and reused with almost no loss of adsorption capacity, which is satisfactory for practical applications.
研究领域:
环境科学
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材料科学
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2025
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IF 7.7
Rong Qin,Mengqi Bai,Feiyi Zhang,Zengkai Wang,Fenglian Liu,Mingdong Dong,Lei Liu,Jian Xu
The detection of circulating tumor cells (CTCs) has emerged as a promising approach for the early diagnosis, therapeutic monitoring, and prognosis of malignant tumors. In this study, we present a novel magnetic nanoparticle (MP) functionalized with dual recognition peptides targeting the epithelial cell adhesion molecule (EpCAM) and human epidermal growth factor receptor 2 (HER2). Additionally, bovine serum albumin (BSA), an anti-fouling agent, is employed onto MP, effectively preventing the non-specific adhesion of platelets and proteins. This multifunctional peptide based magnetic biointerface (MPMB) platform effectively captures breast cancer cells in model systems and isolates CTCs from the blood of breast cancer patients. CTC enumeration of peripheral blood samples from 40 breast cancer patients, 30 benign breast tumor patients, and 30 healthy donors revealed a 70 % positivity rate in breast cancer patients (28/40), 6.7 % in benign breast tumor patients (2/30), and no positivity in healthy donors (0/30). CTC counts were significantly higher in breast cancer patients with advanced stages, particularly in those with distant metastasis, compared to early-stage patients ( p < 0.05). Moreover, among 10 breast cancer patients with positive CTC detection prior to surgery, 6 showed negative CTC results post-surgery. These findings indicate that the MPMB approach provides a simple, flexible, and efficient platform for monitoring therapeutic efficacy and evaluating prognosis in breast cancer patients undergoing treatment.
研究领域:
医学
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生物技术
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产品关联
DOI
高影响因子
ACS Applied Bio Materials
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2021
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IF 0
Guowen Qian,Lemin Zhang,Xia Li,Cijun Shuai,Xiupeng Wang
Magnetite (Fe3O4) nanoparticles as drug carriers can achieve precise drug target due to their magnetic property. However, they are easy to aggregate in the physiological environment, which obviously limits their application in drug delivery. The development of the Fe-MIL-88B-derived method to construct the Fe3O4-loaded mesoporous carbon (Fe3O4/carbon) system is a feasible strategy to solve the issue. First, iron atoms evenly distribute in the organic links through coordination bonds in Fe-MIL-88B. After the carbonization of Fe-MIL-88B, mesoporous carbon acts as a barrier to prevent the aggregation of Fe3O4 nanoparticles. Herein, Fe-MIL-88B particles were fabricated by the hydrothermal method and then pyrolyzed to construct Fe3O4/carbon systems. Results showed that Fe3O4 nanoparticles uniformly in situ grew on mesoporous carbon generated by the carbonization of organic components. More encouragingly, the Fe3O4/carbon system loaded with DOX demonstrated pH-responsive DOX release, efficient delivery of DOX into cancer cells, and significant cancer cell killing ability. Therefore, the Fe3O4/carbon systems prepared by the Fe-MIL-88B-derived method might open up a way for targeted and controlled drug delivery.
研究领域:
材料科学
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生物医学工程
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药学
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产品关联
DOI
PubMed
FOOD CONTROL
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2019
·
IF 4.248
Xueke Li,Qiaoying Ming,Rui Cai,Tianli Yue,Yahong Yuan,Zhenpeng Gao,Zhouli Wang
The pollution of heavy metals in fruit juice is still increasing and becoming a significant food safety issue worldwide nowadays. In this work, twelve strains of lactic acid bacteria (LAB) were evaluated for the biomass and their capability to adsorb Cd 2+ and Pb 2+ . One strain of LAB capable of efficiently adsorbing heavy metals in apple juice was screened. The obtained LAB was functionalized by magnetic Fe 3 O 4 nanoparticles (MNPs) and modified by Ethylenediaminetetraacetic acid dianhydride (EDTAD) for generation EDTAD-MNPs-LAB (EMB) adsorbent, which was further characterized by Scanning electron microscopy, Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. The results indicated that the strain of LAB was successfully functionalized and modified by MNPs and EDTAD. The maximum adsorption capacity of EMB adsorbent for Cd 2+ and Pb 2+ in apple juice was 0.57 mg/g and 0.17 mg/g, respectively. The EMB adsorbent could be rapidly separated from the sample solution with the help of magnetic field and the adsorption process has no significant impact on the quality of fruit juice. The regeneration experiments revealed that the EMB adsorbent could be successfully reused. The research provides a new insight and strategy for the effective adsorption and removal of heavy metals from juice.
研究领域:
食品科学
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环境生物学
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微生物学
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2016
·Vol. 183
·期 8
·2467-2472
·
IF 4.831
Yang Yang,Ma Xiaowei,Feng Fan,Dang Xueping,Huang Jianlin,Chen Huaixia
The article describes the synthesis of core-shell magnetic nanoparticles (MNPs) of the type Fe 3 O 4 @MIL-100 (MIL standing for Material Institut Lavoisier), and their application as sorbent for magnetic solid-phase extraction (MSPE) of triclosan. The MNPs were prepared via circular self-assembly of ferric chloride and benzenetricarboxylic acid. The functionalized MNPs were characterized by transmission electron microscopy, FTIR and thermogravimetry. Following extraction, triclosan was eluted with ammoniacal methanol and then submitted to HPLC with UV detection. The amount of magnetic microspheres, sample pH and ionic strength, adsorption time, desorption time, desorption solvent and the volume of the eluent were optimized. Under optimum conditions, the method showed good linearity in the 0.1 to 50 mg·kg −1 triclosan concentration range in toothpaste samples. Other features include (a) intra-day and inter-day relative standard deviations (RSD, for n = 4) of <5.5 %, (b) a 30 μg·kg −1 limit of detection, and (c) extraction recoveries between 90.86 % and 101.1 %. The method was successfully applied to the determination of triclosan in children’s toothpaste.
The article describes the synthesis of core-shell magnetic nanoparticles (MNPs) of the type Fe 3 O 4 @MIL-100, and their application as sorbent for magnetic solid-phase extraction (MSPE) of triclosan.
研究领域:
分析化学
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环境科学
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材料科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）