SENSORS AND ACTUATORS B-CHEMICAL
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2023
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IF 8.4
Zhujun Ai,Xinmin Li,Rui Chen,Daobin Han,Huijie Bai,Shaoying Yan,Pengcheng Gu,Haiping Wu,Shijia Ding,Junman Chen,Hua Tang
DNA walkers have been widely used to build biosensing systems. However, their moving efficiency was limited, which has hindered practical applications. Here, we for the first time integrated localized CHA with nanoscale AuNPs scaffolds to achieve space double-confined catalytic hairpin assembly (SDCHA) for developing a fast three-dimensional DNA walker. Driven by spatially proximal displacement, the walker possessed a high walking speed, which was about 8 times, 9 times and 23 times faster than LCHA, CHA-AuNPs and conventional CHA, respectively. Moreover, the walking time was about 1.4–8.3 times shorter than most recently reported DNA walkers and space-confined strand displacement reactions. By further integrating it with magnetic probes and g-C 3 N 4 nanosheets , an ultrasensitive and enzyme-free homogeneous electrochemiluminescence (Ho-ECL) aptasensor was firstly constructed for sonic hedgehog (SHh) detection. Profiting from this tailored integration, the Ho-ECL aptasensor avoided multiple interferences from complex milieu as well as tedious procedures of electrode modifying and washing, showing good signal stability, repeatability and reliability. It was also proved to achieve ultrasensitive SHh detection with the detection limit as low as 13.5 fM, superior to previous research on SHh detection. This work might broaden ideas for the construction of fast DNA walkers and facile Ho-ECL bioanalytical methods for clinical diagnosis.
研究领域:
生物传感器
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纳米技术
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电化学
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分子生物学
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分析化学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
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2023
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IF 4.8
Limin Yang,Yiping Zhang,Xingdong Dong,Xuan Wang,Mingming Li,Lei Jiang,Zhen Liu
For electrochemical aptamer analysis based on magnetic beads (MBs), especially for electrochemical impedance analysis, MBs are often directly involved in signal measurements, which may cause high background signals, as well as interference from non-specific adsorption. Herein, a method for the indirect involvement of MBs in impedance aptasensing was developed. The key to this method is the construction of a Strep-Tag system integrated apta-magnetic probe, in which the Strep-tag system was employed as a link between the aptamer and MBs. The acetamiprid-binding aptamer (ABA) was indirectly attached to Strep-Tactin-coated MBs (STMBs), through the hybridization with its complementary strand (cABA), which was covalently conjugated to Strep-tag II. After acetamiprid capture, desthiobiotin triggered the release of Strep-tag II-conjugated cABA, which was subsequently interacted with the ABA-modified electrode for impedance measurements. Moreover, streptavidin-conjugated horseradish peroxidase (streptavidin-HRP) was added to amplify the impedance signal. The changes in electron-transfer resistance (Δ R ct ) are linearly related to the logarithm of acetamiprid concentration in the range of 0.5–10 pM. Detection limit as low as 0.15 pM was achieved. Satisfactory recoveries ranging from 95.25 to 101.2 % with relative standard deviations of 5.12 % were also obtained in analyzing real spiked agricultural products.
研究领域:
分析化学
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电化学
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生物传感器
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农业化学
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产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
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2021
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IF 6.558
Lunjie Huang,Da-Wen Sun,Zhihui Wu,Hongbin Pu,Qingyi Wei
Efficient identification of pathogenic bacteria is greatly concerned with microbial food safety and foodborne diseases diagnosis. Surface-enhanced Raman scattering (SERS) tags are among the cutting-edge tools for bioanalysis, but facing troubles in either SERS sensitivity, durability, interfering signals, or universal recognition agents for target bacteria. This work proposed a multivariate scheme enabled by polyphenolic chemistry for the green synthesis, facile stabilization (functionalization), protective encapsulation, and bio-affinitive design of metal-phenolic networks (MPNs)-encapsulated silver SERS nanotags ( [email protected] @MPNs). With remarkable SERS properties, shelf stability, and bacterial affinity, [email protected] @MPNs tags enabled rapid, reproducible, and interference-free SERS detection of two representative foodborne pathogens ( i.e., E. coli O157: H7 and S. aureus ) in the assistance of an aptamer-labelled magnetic probe, reaching good sensitivity and selectivity. Moreover, this SERS biosensor worked well in real food samples, manifesting the potential of polyphenolic chemistry in the customization of bio-affinitive SERS nanotags for food safety detection.
研究领域:
生物技术
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分析化学
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食品科学
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微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）