Arabian Journal of Chemistry
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2022
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IF 6.212
Jie Zhao,Yan Gong
RNA plays a crucial role in biochemical processes. RNAs with abasic sites (AP-RNA), as one of RNA lesions, will cause serious negative consequences. Efficient and sensitive detection of AP-RNA deserves but does not receive enough attention. Water-soluble uridine triphosphate (UTP)-capped Mn-doped ZnS quantum dots (QDs), which have excellent room-temperature phosphorescence (RTP) properties, were prepared by wet-chemical method. The UTP on the surface of QDs could form a precipitation complex with amiloride (AMI) to quench the RTP. Meanwhile, abasic double-stranded RNA (AP-dsRNA) with a high affinity to AMI could dissociate AMI from the surface of UTP-QDs, resulting in RTP recovery. In this process, a highly sensitive and selective RTP-detection system for AP-dsRNA was constructed with AMI as the modulation factor and UTP-QDs as the luminescence matrix. RTP was turned off and on for the determination of AP-dsRNA, and the interference of background fluorescence and scattered light was effectively avoided during complex formation and aggregation. Steric hindrance of the conformation of AP-dsRNA provided efficient selectivity. The detection limit for AP-dsRNA was 0.86 nM, the relative standard deviation was 2.1%, and the recovery of biological samples with AP-dsRNA addition ranged from 95% to 103% at optimal conditions. The system is expected to provide new methods for environmental monitoring, early disease screening, and evaluation of gene editing efficiency.
研究领域:
生物化学
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分子生物学
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分析化学
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高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 4
·1-10
·
IF 5.479
Luo Shiqing,Miao Yanming,Guo Junping,Sun Xiaojie,Yan Guiqin
Mesoporous molecularly imprinted polymers (MIPs) containing mangnanese-doped ZnS quantum dots (Mn-ZnS QDs) were prepared for specific recognition and detection of 4-nitrophenol (4-NP). The Mn-ZnS QDs display orange room-temperature phosphorescence with excitation/emission peaks at 295/590 nm and a decay time of 2.0 ms. In the presence of 4-NP, the orange phosphorescence is strongly reduced. Phosphorescence drops linearly in the 0.1–100 μM 4-NP concentration range, and the detection limit is 60 nM. The detection limit is far lower than the maximally allowed 4-NP concentrations in surface water and drinking water as specified by the U.S. Environmental Protection Agency. The intraday ( n = 5) and interday ( n = 6) spiked recovery rates were 96.0–104.5% and 97.9–107.9%, respectively, with relative standard deviations of 0.7–4.8% and 1.8–7.5% respectively. These MIPs integrated the characteristic features of phosphorimetry and molecular imprinting. Potential interference by competitive substances, background fluorescence or scattered light are widely reduced.
Schematic presentation of the synthesis of phosphorescent molecularly-imprinted polymers. A novel probe with manganese-doped ZnS quantum dots (Mn-ZnS QDs) and 3-aminopropyl-triethoxysilane (APTES) as functional monomers and tetraethoxysilane (TEOS) as crosslinking agent was prepared for selective phosphorescence detection of 4-nitrophenol (4-NP).
研究领域:
分析化学
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环境科学
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高影响因子
Analytical Methods
·
2013
·Vol. 5
·期 21
·6094-6099
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IF 1.855
Jing Ma,Fang Wang,Meng Fan,Tianyi Wang,Wei Bian,Shaomin Shuang
The room temperature phosphorescence (RTP) of quantum dots (QDs) offers many advantages compared with fluorescence, such as a longer emission lifetime, wider gap between excitation and emission spectra and minimal interference. Hence, the RTP properties of QDs and their potential for phosphorescence detection have drawn more and more attention. In this paper, N-acetyl-L-cysteine (NAC)-capped Mn-doped ZnS QDs were synthesized by a hydrothermal method. The QDs displayed a strong phosphorescence emission peak at 590 nm upon excitation at 315 nm. Because their RTP was efficiently quenched by hydrochlorothiazide (HCT), a novel RTP method for determination of HCT was developed. The results showed that under the optimized conditions, the changes of RTP intensity showed a good linear relationship with the concentration of HCT in the range of 2.5 to 95 μM, with a limit of detection of 1.25 μM and relative standard deviation of 0.44%. The related theories preliminarily demonstrated the dynamic quenching mechanism. In addition, this method was successfully applied to the analysis of practical samples. The recovery of spiked solutions ranged from 94–104%.
研究领域:
分析化学
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材料科学
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高影响因子
Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Wenjuan Dong,Zhuorong Fan,Ziyu Feng,Jia Shi,Xinyu Shi,Shengmei Song,Xiaojuan Gong,Wenting Liang
Phenolic compounds have increasingly serious damage to the environment, and it is imperative to develop new detection means for them. In this study, Mn-doped ZnS quantum dot (Mn/ZnS QDs) were synthesized and conjugated with horseradish peroxidase (HRP) via its ligand N-acetyl-L-cysteine (NAC) to form a bionanoconjugates with the phosphorescent response, named Mn/ZnS QDs-HRP. In the presence of a small amount of hydrogen peroxide, phenolic compounds can quench the phosphorescence of Mn/ZnS QDs-HRP, and the quenching type is speculated to be a synergistic effect of dynamic mechanism and internal filtration effect. Taking catechol as an experimental object, the phosphorescence signal of Mn/ZnS QDs-HRP showed a good linear correlation with the concentration of catechol, and the detection limit was 0.068 μM (S/N = 3). The strong anti-interference ability of room temperature phosphorescence endows the probe with excellent sensitivity, and the specific recognition performance of the enzyme makes it have excellent selectivity. Moreover, the probe exhibits good repeatability and stability. When used for actual sample detection, Mn/ZnS QDs-HRP provides stable and reliable quantification of catechol in different water samples with spiked recoveries of 92.3–104.4 %. This study provides a sensitive, convenient, and efficient phosphorescent response sensor for detecting phenolic compounds, which is of positive significance for environmental protection.
研究领域:
环境科学
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生物化学
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纳米技术
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高影响因子
NEW JOURNAL OF CHEMISTRY
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2019
·Vol. 43
·期 31
·12410-12416
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IF 3.069
Fengyi Wang,Qianqian Peng,Jing Hu,Xuan Hu,Huaqiao Peng,Lin Li,Dan Xiao,Baozhan Zheng,Juan Du
A nanohybrid-based ratiometric phosphorescent assay composed of water-soluble carbon dots (CDs) and water-dispersible lanthanide-doped inorganic nanoparticles CaTiO3:Pr3+@SiO2 is constructed. The as-prepared CDs and CaTiO3:Pr3+@SiO2 nanoparticles were systematically characterized by transmission electron microscopy (TEM), scanning electron microscopy, dynamic light scattering, X-ray diffraction and X-ray photoelectron spectroscopy (XPS). Under a single excitation wavelength at 345 nm, a dual-emission of CDs at 446 nm and CaTiO3:Pr3+@SiO2 nanoparticles at 615 nm are detected, and the long luminescent lifetimes of the two phosphorescent emissions were confirmed by 3-D time-resolved phosphorescence emission spectra. The excellent phosphorescence performance of CDs and CaTiO3:Pr3+@SiO2 nanoparticles is utilized for the development of a ratiometric phosphorescent platform. In the presence of Hg2+, the phosphorescent emission of CDs was dramatically quenched, and that of CaTiO3:Pr3+@SiO2 nanoparticles nearly unchanged. Taking advantage of this, an effective ratiometric phosphorescent sensor unaffected by sensor concentration, background fluorescence and environmental conditions is constructed. Furthermore, the successful application of the nanohybrid phosphorescent system in water and biological samples lays the foundation for building a reliable approach to monitor heavy metal ions in complex environmental and biological samples.
研究领域:
分析化学
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环境科学
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生物医学
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高影响因子
MICROCHIMICA ACTA
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2018
·Vol. 185
·期 4
·1-6
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IF 5.705
Wei Xiao,Yu Miaomiao,Li Chen,Gong Xinghui,Qin Fang,Wang Zhenhong
The authors describe a multifunctional magnetic molecularly imprinted phosphorescent nanoparticle probe for the selective determination of 2,4,6-trichlorophenol (2,4,6-TCP). The probe consists of a magnetite (Fe 3 O 4 ) core that is coated with a molecularly imprinted polymer doped with Mn-doped ZnS quantum dots (QDs). The MIP was obtained by copolymerization of acrylamide, ethylene glycol dimethacrylate, and 2,4,6-TCP. The resulting nanoprobe shows strong phosphorescence (with excitation/emission peaks at 320/594 nm) due to the presence of the QDs, good magnetism, and high selectivity for 2,4,6-TCP. Under optimal detection condition, response is linear in the 0.1–30 μmol L −1 2,4,6-TCP concentration range. The imprinting factor is 8.84, and the detection limit is 35 nmol L −1 . The method was successfully applied to the determination of 2,4,6-TCP in spiked river water and waste water.
Schematic of a multifunctional phosphorescent probe for 2,4,6-trichlorophenol. It consists of a magnetic core coated with a molecularly imprinted polymer shell containing Mn(II) doped ZnS quantum dots whose room-temperature phosphorescence is quenched by 2,4,6-trichlorophenol.
研究领域:
分析化学
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环境科学
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纳米技术
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DOI
PubMed
高影响因子
LUMINESCENCE
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2013
·Vol. 29
·期 2
·151-157
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IF 1.273
Wei Bian,Jing Ma,Qiaoling Liu,Yanli Wei,Yingfu Li,Chuan Dong,Shaomin Shuang
N -acetyl- l -cysteine-capped Mn-doped ZnS quantum dots (QDs) were prepared by hydrothermal methods. It could emit phosphorescence at 583 nm with the excitation wavelength at 315 nm. The phosphorescence intensity of QDs could be quenched dramatically by increasing the concentration of Co 2+ ion. The novel phosphorescence sensor based on N -acetyl- l -cysteine-capped QDs was developed for detecting Co 2+ ion with a linear dynamic range of 1.25 × 10 –6 –3.25 × 10 –5 m . The limit of detection and RSD were 6.0 × 10 –8 m and 2.3%, respectively. Interference experiments showed excellent selectivity over numerous cations such as alkali, alkaline earth and transitional metal ions. The possible quenching mechanism was also examined by phosphorescence decays. The proposed phosphorescence method was further applied to the trace determination of Co 2+ ion in tap and pond water samples with recoveries of 97.75–103.32%. Copyright © 2013 John Wiley & Sons, Ltd.
研究领域:
分析化学
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材料科学
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环境科学
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DOI
PubMed
高影响因子
TALANTA
·
2019
·
IF 4.916
Rong-Hua Wang,Cheng-Long Zhu,Ling-Ling Wang,Li-Zhi Xu,Wen-Long Wang,Cheng Yang,Yi Zhang
An aptamer-based colorimetric-phosphorescent assay was developed for the detection of isocarbophos . The colorimetric assay relied on the aggregation of gold nanoparticles (AuNPs) caused by the competitive binding of aptamer between isocarbophos and AuNPs in the presence of a high salt concentration. The further addition of persistent luminescence nanorods (PLNRs) into the system showed the phosphorescence sensitively proportional to the concentration of isocarbophos, due to the inner filter effect between PLNRs and AuNPs. The assay showed good linearity within 50–500 μg/L and 5–160 μg/L, and limit of detection of 7.1 μg/L and 0.54 μg/L in colorimetry and phosphorescence mode, respectively. The feasibility of this approach for food analysis was demonstrated with the sensitive and selective determination of isocarbophos residues in vegetables.
研究领域:
分析化学
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食品安全
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环境科学
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DOI
PubMed
高影响因子
TALANTA
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2013
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IF 3.498
Wei Bian,Jing Ma,Wenrong Guo,Dongtao Lu,Meng Fan,Yanli Wei,Yingfu Li,Shaomin Shuang,Martin M.F. Choi
N -Acetyl-L-cysteine (NAC) and L-cysteine (Cys) capped Mn doped ZnS quantum dots (NAC-Mn/ZnS QDs and Cys-Mn/ZnS QDs) are firstly prepared by hydrothermal methods . These QDs display strong phosphorescence emission peaks at 583 and 580 nm upon excitation at 315 and 306 nm, respectively. Since their room-temperature phosphorescence is efficiently quenched by L-ascorbic acid (AA), they have been employed as phosphorescence probes for detecting AA. The linear working ranges are 2.5–37.5 and 2.5–47.5 µM and the limits of detection are 0.72 and 1.38 µM for NAC-Mn/ZnS QDs and Cys-Mn/ZnS QDs, respectively. The possible quenching mechanisms have been discussed in detail. The QDs probes are highly selective to AA over other common ions, amino acids , glucose and bovine serum album. Finally, they have been applied successfully for detection of AA in human urine samples with satisfactory results. The recoveries are 98–104%. Our work provides a simple and convenient phosphorescence method to determine AA in real samples.
研究领域:
分析化学
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生物医学
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DOI
PubMed
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
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2017
·
IF 0.795
Dan-Dan TANG,Jin-Yi ZHANG,Xian-Deng HOU,Peng WU
The key to maximize the sensitivity of inner filter effect (IFE)-based assay is to enlarge the overlap between the absorption spectra of the absorber and the excitation and/or emission bands of the fluorophore. In this work, Mn-doped ZnS QDs is chosen for IFE-based detection of β -glucuronidase (GUS), since the excitation spectrum of QDs is perfectly overlapped with the absorption spectrum of the substrate of GUS, namely 4-nitrophenyl- β-D- glucuronide (PNPG). Since absorption spectrum of the enzymatic product of GUS-PNP are almost not overlapped with the excitation and emission spectra of Mn-ZnS QDs, the phosphorescent turn-on determination of GUS can be successfully proposed and carried out. In addition, the excellent room temperature phosphorescence property of Mn-ZnS QDs can eliminate the fluorescence background from biological organisms, which can be effectively applied to biological sample analysis with good anti-interference ability. Accordingly, a simple turn-on phosphorescent assay of GUS was developed with a linear range of 10–300 U L −1 and a limit of detection of 7 U L −1 , and the proposed system was also successfully applied for actual detection of Escherichia coli .
研究领域:
生物化学
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分析化学
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生物医学工程
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高影响因子
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