JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
He Mei,Jingcheng Huang,Guixin Wang,Kun Chen,Huili Wang,Xuedong Wang,Wenwei Wang
Herein, we engineered a novel pH-responsive ratiometric assay by virtue of the composite system of Ce 3+ -enhanced glutathione-encapsulated copper nanoclusters (GSH-CuNCs-Ce 3+ ) and N-doped carbon quantum dots (N-CQDs). The GSH-CuNCs-Ce 3+ and N-CQDs were fabricated using one-pot chemical reduction and hydrothermal approaches, respectively, and their morphologies as well as physical and chemical properties were testified in detail by a series of characterization techniques. At 350 nm excitation wavelength, the GSH-CuNCs-Ce 3+ /N-CQDs system (GCC-NC) featured the 440/650 nm dual-emitting property. The ratios of fluorescence intensities (F 650 /F 440 ) demonstrated a strong pH-dependence from 3.0 to 5.0 with a coefficient of determination of 0.9982. Consequently, the GCC-NC system was feasible for constructing a pH-responsive dual-emitting fluoroprobe. Based on the catalytic effect of acetylcholinesterase on acetylcholine to yield acetic acid and further trigger varying solution pH, the as-constructed GCC-NC fluoroprobe was satisfactorily applied for detecting malathion . Under optimized conditions, the newly developed GCC-NC fluoroprobe supplied a wider linear range (0.25–200 μM), lower detection limit (0.075 μM), satisfactory recoveries (96.3–106.2%) and higher precision for malathion in several kinds of fruit juice samples. The fortified experiments by malathion’s structural analogues and metal ions provided compelling evidence that this fluoroprobe had strong anti-interference capacity and high specificity for malathion. These findings render us to believe that the as-constructed pH-responsive dual-emitting fluoroprobe holds great promise in trace malathion assay in food matrices.
研究领域:
分析化学
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食品安全
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Ruiqi Zou,Junxiao Shi,Qi Lu,Chunyan Sun,Haiqing Ye,Xu Yan,Fangjie Tian,Hongxia Li
Given the potential dangers of organophosphorus pesticides to food safety and human health, the development of a reliable and precise detection platform for pesticides is essential. In this study, we present a novel ‘armor-plating’ laccase-mimetic catalyst (DNA-Cu@MOFs)-based colorimetric platform, which enables stable and selective pesticide detection. The DNA-Cu@MOFs enhance catalytic stability and overcome pH limitations, enabling effective catalysis under neutral and alkaline physiological conditions, making them well-suited for practical applications in biosensor development. By combining the catalytic properties of DNA-Cu@MOFs with a high-affinity biorecognition element (acetylcholinesterase), the platform achieves a linear detection range of 3.0–90 ng mL −1 for chlorpyrifos, with a detection limit of 0.75 ng mL −1 . Notably, this platform demonstrates significant stability in chlorpyrifos detection even in the presence of environmental interferents. This robust colorimetric platform offers new possibilities for pesticide detection and provides a solid foundation for the development of comprehensive and accurate pesticide monitoring systems.
研究领域:
生物传感
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环境科学
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食品科学
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产品关联
DOI
PubMed
高影响因子
ACS Sensors
·
2025
·
IF 9.1
Yuanyuan Lu,Tingting Ma,Menghuan Zhang,Qingwen Lan,Jiao Yang,Xinqiang Liang
Accurate detection of bioavailable phosphorus (BP) is critical to mitigate freshwater eutrophication from excess phosphorus, but quantifying trace soluble reactive phosphorus (SRP) under real-world pH remains challenging. We present a portable paper-based electrochemical sensor for simultaneous SRP and pH monitoring in agricultural water. The platform establishes dual linear correlations: peak current–SRP concentration (LOD < 2 μM) and peak potential–pH (1–7) via square wave voltammetry (SWV). This enables micromolar-level SRP quantification across an environmentally relevant pH range with minimal interference. Field validation in farmland solutions demonstrates direct differentiation of dissolved and colloidal phosphorus fractions without pH alteration, overcoming the limitations of conventional methods. This work provides a powerful tool for in situ environmental monitoring, offering new insights into P bioavailability and advancing sustainable nutrient management in diverse agricultural contexts.
研究领域:
环境科学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Xiaomin Pang,Yongfeng Chen,Rui Gao,Yufeng Sun,Xuguang Qiao,Zhixiang Xu
Herein, a novel electrochemical sensor based on zirconium-doped cobalt oxyhydroxide (ZrCoOOH) was proposed for highly sensitive non-enzymatic determination of malathion (MAL). The doping of Zr can improve the electrical conductivity of CoOOH, of which the transfer resistance was reduced from 241.1 Ω to 140.2 Ω. Furthermore, the X-ray photoelectron spectroscopy confirmed that part of Co 2+ was converted to Co 3+ due to the introduction of Zr. The Co 3+ in ZrCoOOH could react with MAL to form Co 2+ , which enhanced the electrooxidation current of Co 2+ . Therefore, the peak current of Co 2+ was served as detection probe for MAL. Under optimal conditions, the developed sensor established the linear relationship for MAL in the concentration range of 0.001–10.0 μM with a low limit of detection (0.64 nM). The constructed sensor was employed to detect MAL in food samples (peach, kiwi fruit, spinach and tomato), verifying the accuracy and practicability of the sensor.
研究领域:
分析化学
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环境科学
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Xiaoxiao Dong,Chang Lu,Shuaijie Guo,Siqi Zhang,Xuelian Hu,Wenliu Wang,Yiwei Tang,Shuo Wang,Xiuying Liu
A magnetic NiFe 2 O 4 @TiO 2 @Au composite was successfully synthesized via layer-by-layer assembly approach and exhibited excellent SERS performance. The composite was functionalized with an aptamer modified with Cy3, serving as the Raman signal reporter, and was hybridized with complementary DNA oligonucleotides (cDNA) to construct the NiFe 2 O 4 @TiO 2 @Au@ssDNA SERS aptasensor. This aptasensor enabled the development of a sensitive, specific, and straightforward SERS assay for acetamiprid detection, exhibiting good accuracy. Under optimized conditions, an inverse correlation was established between the acetamiprid concentration and the Raman intensity at the characteristic peak of 1132 cm −1 , spanning a range of 10 −1 -10 4 nmol/L with a LOD of 8.4 × 10 −1 pmol/L. Standard addition experiments to three types of leafy vegetables samples yielded recoveries ranging from 94.30 % to 111.83 %, with a relative standard deviation of <5.56 %. This method shows potential for detecting other hazardous substances in food and environmental samples by simply changing the corresponding aptamers and cDNA.
研究领域:
分析化学
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食品安全
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Jingfang Guo,Feng Zhao,Zhenhui Yue,Zhen Lei
Rapid and accurate detection of carbosulfan residues in vegetables is important for ensuring food safety. Herein, based on the unique hydrolysis behavior of carbosulfan, a nanozyme-based colorimetric sensing platform was proposed for detection of carbosulfan. Hollow polydopamine/MnO 2 nanoparticles (H-PDA/MnO 2 NPs) with excellent oxidase-like activity were synthesized, which can promote the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) into blue oxidative product (oxTMB). Under acidic conditions, carbosulfan can be decomposed and produce reductive sulfide species (-SH), which are capable to disintegrate MnO 2 NPs, resulting in decreased oxidase-like activity of H-PDA/MnO 2 NPs. Based on the inhibitory effect on oxidase activity of H-PDA/MnO 2 NPs, an acetylcholinesterase-free colorimetric assay was proposed for detection of carbosulfan with low limit of detection of 0.63 ng mL −1 . Integrating test swabs with smartphone, a portable colorimetric sensor was constructed, showing great potential for on-site detection. To demonstrate the feasibility of this method, carbosulfan in real vegetable samples were determined.
研究领域:
食品安全
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分析化学
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
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2025
·
IF 1.3
Aiying SONG,Rong LIU,Xinghe HE,Linlin WEI
In the field of forensic sciences, the analysis of amphetamine-type stimulants in hair samples has become a fundamental tool for assessing long-term substance use. The persistent nature of amphetamine-type stimulants in hair provides a unique chronological record of drug intake, offering valuable insights for legal and clinical applications. Developing a simple, sensitive, and reliable method for the determination of these drugs in hair samples remains an important issue in forensic analysis. In this study, polydimethylsiloxane modified stainless steel fiber was firstly fabricated. By taking advantage of the affinity of the polydimethylsiloxane coating toward amphetamine-type stimulants and the excellent conductivity of stainless steel wire, an electro-enhanced solid-phase microextraction coupled with gas chromatography equipped with a nitrogen-phosphorus detector method was established to sensitively determine four common amphetamine-type stimulants in hair samples. The experimental results showed a linear range from 0.1 to 50 µg L⁻¹. The limits of detection and qualification respectively varied from 0.012 to 0.039 and 0.05 to 0.13 µg L⁻¹ ( S / N = 3), and it exhibited good reproducibility (< 6.2%). Recoveries ranging from 79% to 106%, with a relative standard deviation of < 6.7%, were obtained for all analytes. Fiber-to-fiber reproducibility (RSD), obtained using three fibers, was < 8.8%. The developed strategy combined the affinity of the polydimethylsiloxane coating towards amphetamine-type stimulants with the electrical conductivity of the stainless steel wire, effectively improving the enrichment ability and detection sensitivity, and achieving simultaneous selective enrichment and detection of several analytes. This is a simple, efficient, and reliable method for the analysis of amphetamine-type stimulants from complex matrices.
研究领域:
法医学
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 11
·1-12
·
IF 5.3
Wen Peng,Su Hao,Yin Wen-jia,Hu Jian-cheng,Wang Yu,Yang Jin-yi,Xiao Zhi-li,Xu Zhen-lin,Shen Yu-dong,Wang Hong,Hammock Bruce D.
A new type of label-free electrochemical immunosensor for the high-sensitivity determination of parathion was developed based on the oriented immobilization of nanobody (VHH9) on a gold nanoparticle-loaded polyvinyl alcohol/citric acid nanofiber membrane-modified electrode. The morphology characterization and assembly process of the modified materials were investigated using scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). Under the optimum conditions, the label-free electrochemical immunosensor for parathion exhibited a linear range of 0.0015–6400 ng/mL and a low detection limit of 0.48 pg/mL, the signal response of which was 10 times higher than that of the randomly immobilized VHH9. The immunosensor possessed high selectivity, good repeatability and reusability (keeping above 90% of its initial activity after repeating 8 times), and stability (remaining 90% after 9 weeks of storage). Finally, the average recoveries of parathion from food samples were 93.76–105.73% with the coefficient of variation being 2.65–6.85%, showing good correlation with UPLC ( R 2 = 0.9950). Therefore, our nanobody immobilization protocol is simple and effective and proves the potential to be utilized as a promising candidate for sensing platform. Graphical
研究领域:
生物传感
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环境监测
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食品检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
·
2025
·
IF 5.9
Zhenpeng Men,Ragini Singh,Bingyuan Zhang,Santosh Kumar
In this paper, we introduce a seven-core tapered optical fiber biosensor with high sensitivity for label-free detection of paraoxon compounds that utilizes the localized surface plasmon resonance (LSPR) effect. By its unique circle-M-circle (CMC) structure surrounding the fiber surface, it achieves an obvious amplification of evanescent field at the sensor and improves sensitivity greatly. Finally, the optimized tapered shape enhances surface plasmon light interaction and hence increases sensitivity to low concentrations of paraoxon residues. Since gold nanoparticles (AuNPs) are used as an active probe for surface modification, the optical properties of Au make them have a good LSPR effect. We also employ, as auxiliary materials, tungsten disulfide (WS2) thin layers and ZnO nanowires (NWs), of which the biocompatibility advantage can reinforce together with stability for detection probes to the WaveFlex sensor. The results of the experiment show a high sensitivity for detecting paraoxon residues in agricultural products: 33.8 ng/mL and a detection limit of 6.83 μg/mL. The developed biosensor enables portability through miniaturization and also maintains its performance stability in various environmental conditions, which can be considered a powerful tool for field monitoring of paraoxon to enhance both environmental and food safety assessments. The sensor has shown favorable practical applicability due to at least desirable selectivity and repeatability. The CMC-like optical fiber-based WaveFlex biosensor being developed based on LSPR detection represents a great progress of sensor-based technology for environmental monitoring with wide application scenarios, which serves as an efficient and effective surveillance mechanism for point-of-sampling detection to the timely-onset paraoxon analysis.
研究领域:
环境科学
·
生物传感技术
·
食品安全
✓ 已验证
产品关联
DOI
高影响因子
Biosensors-Basel
·
2025
·Vol. 15
·期 1
·64
·
IF 5.6
Yajun Wu,Dongxiao Ma,Xiaoli Zhu,Fangquan Xia
Rapid, effective, and cost-effective methods for large-scale screening of pesticide residues in the environment and agricultural products are important for assessing potential environmental risks and safeguarding human health. Here, we constructed a novel aggregation-induced emission (AIE) electrochemical aptamer (Apt) sensor based on red-emissive sulfur quantum dots (SQDs), which aimed at the rapid screening and quantitative detection of malathion. SQDs were prepared using a two-step oxidation method with good electrochemiluminescence (ECL) optical properties. These SQDs were modified onto the electrode surface to serve as ECL luminophores. Subsequently, Apt was introduced and modified to form a double-helix structure with the complementary chain (cDNA). The ECL signal was reduced because the biomolecules had poor electrical conductivity and inefficient electron transfer. When the target malathion was added, the double helix structure was unraveled, the malathion Apt fell off the electrode surface, and the ECL signal was restored. The linear range of detection was 1.0 × 10 −13 –1.0 × 10 −8 mol·L −1 , and the detection limit was 0.219 fM. The successful preparation of the sensor not only develops the ECL optical properties of SQDs but also expands the application of SQDs in ECL sensing.
研究领域:
分析化学
·
环境科学
·
生物传感技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Environmental Science-Nano
·
2020
·Vol. 7
·期 3
·753-763
·
IF 7.683
Shi-Hua Chen,Xiang-Yu Xiao,Pei-Hua Li,Yi-Xiang Li,Meng Yang,Zheng Guo,Xing-Jiu Huang
The detection of chlorpyrifos is of great importance due to its massive usage and serious toxic effects on human health and the ecosystem. Although fruitful achievements have been made in the photoelectrochemical (PEC) sensing of chlorpyrifos, the construction of a label-free PEC interface with high sensitivity and selectivity still faces serious challenges. In this work, we design a direct Z-scheme ZnS/Co9S8 heterojunction with hollow cubic structures, and the PEC sensor based on such materials achieves the highly sensitive and selective detection of chlorpyrifos through the inhibition to the photoresponse of ZnS/Co9S8 by chlorpyrifos. For sensitivity, the hollow cubic structures of the heterojunction increase the light absorption, and the formation of a direct Z-scheme of the photocatalysts greatly improves the spatial separation of photogenerated charge carriers, thus achieving better PEC activity and higher photocurrent response of the heterojunction than those of individual materials. It shows a wide linear range from 0.05 ppb to 40 ppb with a detection limit as low as 0.0166 ppb (defined as S/N = 3). In particular, the specific interaction between ZnS/Co9S8 and N and S atoms of chlorpyrifos creates the high selectivity for chlorpyrifos. In addition, the PEC sensor is still stable after storage for two weeks.
研究领域:
环境科学
·
分析化学
·
材料科学
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Qian Liu,Yuanyuan Yin,Nan Hao,Jing Qian,Libo Li,Tianyan You,Hanping Mao,Kun Wang
This work presents a selective and reliable visible-light driven photoelectrochemical (PEC) chlopyrifos sensor using nitrogen functionalized graphene quantum dots/3D bismuth oxyiodine (NFGQDs/3DBiOI) hybrid hollow microspheres (HHMs) as a remarkable photoelectrode. In this protocol, a facile one-pot solvothermal reaction was used for the synthesis of the new NFGQDs/3DBiOI HHMs, which achieved significant enhancement (ca. 18-fold) photocurrent generation compared to pure BiOI microspheres by virtue of the band gap narrowing and the electron transfer acceleration of such HHMs. In addition, the introduction of chlorpyrifos pesticide enabled the formation of Bi-chlorpyrifos complex which resulted in quenching the charge transfer efficiency, thus leading to a decrease in photocurrent intensity and selective PEC recording of chlorpyrifos level. This novel PEC sensor exerted predominant performance for chlorpyrifos detection including wide linear range (0.1–50 ng mL −1 ), low detection limit (0.03 ng mL −1 ), satisfactory selectivity and also good feasibility in real water sample analysis. The strategy presented here implying that a three-dimensional hollow nanostructure would play a crucial role in developing multifarious novel detection methodologies in PEC sensing fields.
研究领域:
材料科学
·
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）