BIOCHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 3.7
Qian Zhou,Neng-Bing Long,Rui-Feng Zhang
A new epoxy-based polymerizing/compositing synchronic process was carried out in 3D structure of a melamine sponge. The cured polymer formed a porous and wave-shaped coating on the surface of skeleton in a uniform way. The obtained composite sponge was used as a microflow support for the immobilization of phospholipase A 1 (PLA 1 , from Thermomyces lanuginosus/Fusarium oxysporum ) by co-adsorption/cross-linking with polyethylenimine (PEI). The loading amount and the specific activity of immobilized PLA 1 under the optimal immobilization conditions were 45.7 mg/g support and 9.1 U/mg protein . The PLA 1 -immobilized microflow supports were placed in a columnar reactor, in which transesterification was operated in continuous circulation way. The reaction produced DHA/EPA-incorporated phosphatidylcholine , the hydrolysis side reaction could be inhibited by removal of free water in the reaction medium. Under optimized conditions the incorporation percentage of DHA/EPA and PC yield of 54.3% and 82.4% within 18 h at 55 ℃, respectively. After 10 cycles, the immobilized PLA 1 retained 71.2% of the original activity. The use of microflow support has an advantage on easier scaling up the reactor size towards much larger productivity and higher efficiency.
研究领域:
生物化学
·
酶工程
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DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Zhong Li,Huilin Zhou,Xuan Liu,Weifei Wang,Dongming Lan,Yonghua Wang
The limited availability of phospholipase A 1 (PLA 1 ) has posed significant challenges in enzymatic degumming. In this study, a novel PLA1 (UM2) was introduced to address this limitation, which had a unique thermo-responsive ability to switch phospholipase and lipase activities in response to temperature variations. Remarkably, UM2 displayed an unprecedented selectivity under optimized conditions, preferentially hydrolyzing phospholipids over triacylglycerols—a specificity superior to that of commercial PLA 1 . Moreover, UM2 demonstrated high efficiency in hydrolyzing phospholipids with a predilection for phosphatidylcholine (PC) and phosphatidylethanolamine (PE). A practical application of UM2 on crude flaxseed oil led to a dramatic reduction in phosphorus content, plummeting from an initial 384.06 mg/kg to 4.38 mg/kg. Broadening its industrial applicability, UM2 effectively performed enzymatic degumming for other distinct crude vegetable oils with a unique phospholipid composition. Collectively, these results highlighted the promising application of UM2 in the field of oil degumming.
研究领域:
生物化学
·
食品科学
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DOI
PubMed
高影响因子
Foods
·
2023
·Vol. 12
·期 6
·1289
·
IF 5.2
Xiao-Yan Liu,Wei Chen,Cheng-Tao Wang
Egg white (EW) is frequently used in bakery products because of its excellent foaming capabilities. However, egg yolk (EY) contamination often degrades the foaming characteristics of EW. The purpose of this study was to investigate the effect of different concentrations of phospholipase A1 (PLPA1) and lipase (LP) on EW. The changes in particle size distribution and potential before and after enzymatic digestion of EW with contaminated 0.5 wt% and 1.0%wt EY were tested. The foaming rate and foam stability were measured after the dispersions were digested with different concentrations of PLPA1 and LP. Additionally, the dispersion samples were used to prepare batter and angel cake, and the modulus, density, and microstructure of the batter were analyzed. Results showed that the potential absolute value increased when the EY was hydrolyzed by PLPA1. The distribution of yolk particle size showed a new aggregation and the average particle size decreased after LP hydrolysis. The dispersion samples hydrolyzed by PLPA1 and LP recovered all the properties of the samples at enzymatic concentrations of 500 U/g and 2500 U/g. This may be attributed to the changes in yolk particles resulting from the enzymatic digestion of EY and the production of amphiphilic lysophospholipids, fatty acids, and glycerol.Keywords:egg white;foaming properties;yolk-contaminated;lipase enzymolysis
研究领域:
食品科学
·
化学
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DOI
PubMed
高影响因子
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
·
2021
·Vol. 59
·期 1
·317-326
·
IF 2.701
Weining Wang,Tang Honglin,Chen Yan,Liu Jingyang,Yu Dianyu,Yang Fuming,Elfalleh Walid
In this paper, the free Phospholipase A 1 (PLA 1 ) was immobilized on a magnetic carrier. The average particle diameter of the magnetic carrier was 97 ± 1.3 nm, and the average particle diameter of the magnetically immobilized PLA 1 was 105 nm ± 1.3 nm. The enzyme activity was 1940.5 U/g. The magnetic enzyme was chemically modified with formaldehyde, dextran-aldehyde, and dextran-aldehyde-glycine. The proportions of primary amino groups in the modified magnetic immobilized enzyme PLA 1 were 0, 53.5% and 47.3%, respectively. The optimum pH of the enzyme after chemical modification was 6.5. When the system temperature was 60 °C, the magnetically immobilized PLA 1 modified with dextran-aldehyde-glycine had the optimal activity and stability. This chemically modified magnetic immobilized PLA 1 was applied to soybean oil degumming at 60 °C, 6.5 h (reaction time), and 0.10 mg/kg (enzyme dosage). The phosphorus content in the degummed oil was 9.2 mg/kg. The relative enzyme activity was 77.6% after 7 reuses which would be potentially advantageous for industrial applications.
研究领域:
生物化学
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食品科学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Xuan Liu,Chunjin Huang,Dongming Lan,Weifei Wang,Yonghua Wang
Degumming is essential to eliminate undesired phospholipids from crude vegetable oils before being processed into high-quality products. The degumming efficiency is hampered by the separation of oil and water. To overcome this problem, we introduced an innovative enzymatic degumming strategy for various vegetable oils (rice bran, peanut and flaxseed oil) by combining phospholipase A1 with monoacylglycerol lipase. Compared to using phospholipase A1, the combination of the two enzymes significantly reduced phosphorus content, indicating better hydrolysis of phospholipids. Additionally, the primary hydrolysates by the combined enzymes were glycerylphosphorylcholine and glycerylphosphorylethanolamine that were more affinitive to water to improve oil-water separation and oil yield. Characteristic markers obtained from Partial Least Squares Discriminant Analysis model confirmed the advantages of combined enzymes over single enzyme. This advantage of combined enzymes was further validated in a simulated degumming process. Our findings provide a new enzymatic degumming strategy to expand the toolkit for crude oil refinery.
研究领域:
食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
BIOTECHNOLOGY PROGRESS
·
2022
·Vol. 38
·期 4
·e3256
·
IF 2.909
Duanhao Wang,Meijing Guo,Jiachen Li,Binglin Li,Jiao Wang
Six kinds of Mo-basing nanomaterials (MoO 3 , MoO 3 @Ru, Mo-PDA, MoP C , MoP, CNT@MoS 2 ) were successfully synthesized, which were employed as carriers to immobilize phospholipase A 1 (PLA1) for the hydrolysis of phospholipids (PLs). PLA1 was immobilized by a simple adsorption-precipitation-cross-linking to form an “enzyme net” covering on nanoparticles. The greatest advantage of these nanoparticles was their strong hydrophilic surface. It not only permitted their dispersion in the aqueous phase, but also showed the strong affinity for PLs in the organic phase, because amphiphilic PLs had the polar head group and higher hydrophilicity than other oils components. Michaelis–Menten analysis revealed that higher catalytic activity and enzyme−substrate affinity were observed in several immobilized PLA1 than its free form. MoO 3 was confirmed to be the best candidate for carrier. The highest specific activity of MoO 3 -immobilized PLA1 reached 43.1 U/mg, which was about 1.8 times higher than that of free PLA1 (24.4 U/mg). In addition, the stability and recycling were also enhanced. The robust immobilized PLA1 was prepared in this work, showing great potential for the enzymatic degumming.
研究领域:
生物化学
·
纳米生物学
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食品科学
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DOI
高影响因子
Engineering
·
2025
·
IF 11.6
Zhonghui Liu,Yi Zhang,Tieliang Liu,Qi Zhou,Yandaizi Zhou,Kheng-Lim Goh,Siew-Young Quek,Fenghong Huang,Mingming Zheng
Algae oil contains polyunsaturated fatty acids (PUFAs) that are beneficial to human health and development. Enzymatic refining is a sustainable approach that can improve the quality and nutritional value of algae oil. However, this approach involves the use of specific enzymes to remove unwanted impurities such as phospholipids (PLs) and free fatty acids (FFAs). This study proposes a novel immobilized phospholipase, immobilized phospholipase A1 (PLA1@MCM-41-C 8 ), which serves a dual purpose: it is effective for both degumming arachidonic acid (ARA)-enriched algal oil and its subsequent deacidification. The use of PLA1@MCM-41-C 8 achieved a degumming rate of 95.9% for crude algal oil, with an ARA retention rate of up to 97.3%. The acidity of the degummed oil decreased from 14.5 to 1.1 mg KOH∙g −1 , while simultaneously producing 29.2% high-value diacylglycerol (DAG). By comparing enzymatically degummed and deacidified ARA-enriched algae oil (EDDO) with crude ARA-enriched algae oil (CO), commercialized refining algae oil (CRO), it was found that enzymatic refining had minimal impact on antioxidant stability, fatty acid composition, and oil content. In particular, the flavor characteristics of algal oil before and after enzymatic refining with PLA1@MCM-41-C 8 remained unchanged and were dominated by the main volatile compounds: alcohols and aldehydes. Compared with free phospholipase A1 (PLA1), PLA1@MCM-41-C 8 exhibited significantly improved thermostability, pH tolerance, solvent tolerance, and long-term reusability. These characteristics make it a promising candidate for complex oil refining in industrial applications.
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DOI
高影响因子
Molecular Catalysis
·
2020
·
IF 3.687
Jia-Qin Wu,Ding-Lin Wang,Xiao-Mei Xu,Neng-Bing Long,Rui-Feng Zhang
Novel nano-composites were prepared by coating epoxy resin-based cationic polymer in nano-thickness via in-situ curing on the wall of macroporous SiO 2 with pore size of 0.5∼1 μm. By changing the thickness of polymer coating the porosity and specific surface area varied in range of 90.4∼83.9 % and 115∼74 m 2 /g, respectively. Through electrostatic adsorption phospholipase A 1 (PLA 1 ) was efficiently immobilized on the nano-composited support, the loading amount of PLA 1 on the nano-composited support reached to a maximum of 138.6 mg/g support , the specific activity of the immobilized PLA 1 reached as high as 21,300 U/g protein . The immobilized PLA 1 was applied for the highly efficient synthesis of DHA/EPA-rich phosphatidylcholine . The incorporation of DHA/EPA reached as high as 74.8 % and the yield of phosphatidylcholine (PC) was 83.1 % within 20 h at 60 °C using PC/ethyl esters mass ratio of 1:6. After 12 cycling reuses the immobilized PLA 1 still retained 57.8 % of the incorporation of DHA/EPA.
研究领域:
生物化学
·
食品科学
·
酶工程
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产品关联
DOI
高影响因子
Molecular Plant
·
2022
·
IF 21.949
Chenglin Jiang,Ju Sun,Rui Li,Shijuan Yan,Wei Chen,Liang Guo,Guochen Qin,Pengcheng Wang,Cheng Luo,Wenjie Huang,Qinghua Zhang,Alisdair R. Fernie,David Jackson,Xiang Li,Jianbing Yan
Haploid induction (HI) is an important tool in crop breeding. Phospholipase A1 ( ZmPLA1 ) /NOT LIKE DAD ( NLD ) /MATRILINEAL ( MTL ) is a key gene controlling HI in maize; however, the underlying molecular mechanism remains unclear. In this study, to dissect why loss of ZmPLA1 function could mediate HI we performed a comprehensive multiple omics analysis of zmpla1 mutant anthers by integrating transcriptome , metabolome , quantitative proteome, and protein modification data. Functional classes of significantly enriched or differentially abundant molecular entities were found to be associated with the oxidative stress response, suggesting that a reactive oxygen species (ROS) burst plays a critical role in HI. In support of this, we further discovered that a simple chemical treatment of pollen with ROS reagents could lead to HI. Moreover, we identified ZmPOD65 , which encodes a sperm-specific peroxidase , as a new gene controlling HI. Taken together, our study revealed a likely mechanism of HI, discovered a new gene controlling HI, and created a new method for HI in maize, indicating the importance of ROS balance in maintaining normal reproduction and providing a potential route to accelerate crop breeding.
研究领域:
作物育种
·
分子生物学
·
遗传学
·
生物化学
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Xuefeng Li,Jun Wei,Yufei Zhang,Yuanzhi Xu,Wenlong Yuan,Lin Mei,Shengjiao Yu,Mingming Zheng
This study reports the synthesis of bifunctional hollow double-layered mesoporous silica (HdlMS) nanoparticles as an effective support for enzyme catalysis. Using a self-template method combined with sol–gel processing, selective etching, and surface modification with alkyl groups and nitrogenous fatty amine groups, we successfully prepared the NH2/C8-HdlMS carrier. Phospholipase A1 was then immobilized onto this carrier to construct PLA1@NH2/C8-HdlMS, which was subsequently applied to the synthesis of docosahexaenoic-acid-enriched phosphatidylcholine. The results demonstrated a docosahexaenoic acid incorporation rate of 67% within 8 h, and a catalytic efficiency (CE) of 18.84 mmol·g–1·h–1, the highest value reported to date. The biocatalyst retained approximately 81% of its initial activity after nine consecutive cycles, demonstrating excellent stability and reusability. The HdlMS structure, with its large internal surface area and optimized substrate transport, combined with surface modifications that enhance enzyme–support interactions, contributed to the high CE value, as confirmed by the detailed controlled experiments. This study not only offers novel design principles for high-performance enzyme immobilization carriers but also advances the development of structurally tailored phospholipids for industrial applications.
研究领域:
纳米材料
·
生物催化
·
酶工程
·
材料科学
·
食品化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2016
·
IF 3.782
Ruipu Xin,Faez Iqbal Khan,Zexin Zhao,Zedong Zhang,Bo Yang,Yonghua Wang
The mechanism of lipase binding to the lipid-water interface is crucial for substrate specificity and kinetic properties. In this study, the chain-length specificity, regiospecificity and substrate specificity of Phospholipase A 1 (PLA 1 ) and its parent enzyme Thermomyces lanuginosus lipase (TLL) have been investigated using a classical emulsion system. The results show that both PLA 1 and TLL are 1,3-regioselective lipases. Additionally, the hydrolytic activity of PLA 1 is comparatively lower on short-chain triacylglyceride (TAG) and higher on phosphatidylcholine (PC) than the hydrolytic activity of TLL. Further, the results obtained with monolayer film techniques demonstrate that the C-terminal region regulates the binding of PLA 1 to PC. A hypothesis is presented according to which the α9 helix of C-terminal region in PLA 1 not only controls the opening of lid but also serves as a membrane anchor that assists in binding to PC. These findings bring new insight into rational design of novel lipases with intriguing functionalities.
研究领域:
酶学
·
生物化学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
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