EUROPEAN JOURNAL OF INORGANIC CHEMISTRY
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2022
·Vol. 2022
·期 20
·e202200202
·
IF 2.551
Zicong Tan,Ying Wang,Jie Zhang,Zhang Zhang,Samantha Sze Man Wong,Shiqing Zhang,Hongyan Sun,Ken Kin Lam Yung,Yung-Kang Peng
Graphical CeO 2 nanosphere enclosed by (100) and (111) surfaces has been reported to mimic phosphatase and peroxidase by catalyzing substrate dephosphorylation and oxidation (with H 2 O 2 ). We herein successfully boost the reaction specificity for this material by shape control. The acidic Ce species on octahedron (111) surface was found to selectively catalyze substrate dephosphorylation while its electron rich counterpart on cube (100) surface only promotes substrate oxidation. Among reported nanozymes, CeO 2 seems to be the only transition metal oxide that can mimic phosphatase and peroxidase by catalyzing substrate dephosphorylation and oxidation (with H 2 O 2 ). However, no consensus on the key Ce species was reached in the literature using spherical CeO 2 enclosed by (111) and (100) surfaces, not to mention the further control of its reaction specificity. In this study, octahedral and cubic CeO 2 preferentially terminated by (111) and (100) surfaces were found to exhibit high reaction specificity (and activity) towards each of the above reactions. Spectroscopic evidence suggests that this is closely associated with the Lewis acidity (or electron density) of surface Ce species. The acidic Ce species on (111) surface can catalyze substrate dephosphorylation at room temperature but do not for substrate oxidation with H 2 O 2 . This correlation was further evidenced by the electron-rich Ce species on (100) surface, hindering the first reaction while promoting the latter.
研究领域:
材料科学
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生物化学
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DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2025
·
IF 5.4
Ge Chen,Xiufeng Wang,Wenhui Ai,Wei Zhao,Ting Zhou,Zhiqing Zhang,Fang Wang,Guodong Zhang
Chronic overexposure to aluminum ions can cause damage to human brain and nervous system. In this work, a new strategy for colorimetric and fluorescent dual-mode detection of aluminum ions (Al 3+ ) was developed, which based on the excellent phosphatase-like activity of histidine-functionalized manganese-doped cerium dioxide nanoparticles (Mn-CeO 2 NPs). Water-soluble, uniform and highly dispersible Mn-CeO 2 NPs were prepared by a green and simple one-pot synthesis method. And the NPs with phosphatase-like activity can catalyze the dephosphorylation of phosphate substrates p-nitrophenyl picolinate (p-NPP) and 4-methylumbelliferyl phosphate (4-MUP) to obtain products with UV–vis and fluorescence signals. Interestingly, Al 3+ can chelate with O atoms on the surface of nanozymes, causing them to aggregate, and thereby reducing their catalytic activity. Based on this principle, a simple, sensitive colorimetric and fluorescent dual-mode sensor for detecting Al 3+ was constructed, and the response time could be as short as 1 min. The linear ranges of colorimetric and fluorescent analysis of Al 3+ were 1–30 μM and 1–15 μM, and the limits of detection (LOD) were 0.86 μM and 0.29 μM, respectively. This study not only synthesized an efficient phosphatase-like nanozyme by defect engineering, but also opened up a new channel for sensitive and rapid detection of Al 3+ .
研究领域:
分析化学
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纳米技术
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生物传感
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产品关联
DOI
高影响因子
CHINESE CHEMICAL LETTERS
·
2017
·
IF 1.932
Wei Song,Hai-Juan Zhang,Ying-Hua Liu,Cui-Ling Ren,Hong-Li Chen
A new facile fluorescence probing strategy, which was based on N -doped carbon dots (NCDs) and methyl parathion hydrolase (MPH), was developed for the determination of parathion-methyl (PM). The fluorescence intensity of NCDs-MPH system was proportional to PM concentration in the range of 2.38–73.78 μmol/L, with a detection limit of 0.338 μmol/L. Moreover, the present simple and facile method could be used to determine methyl parathion in environmental and agricultural samples successfully. Furthermore, the detection mechanism of this system is inner filter effect and molecular interactions between NCDs and p -nitrophenol, which is the hydrolysis product of PM catalyzed by methyl parathion hydrolase.
研究领域:
环境科学
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分析化学
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生物化学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Zhen Wu,Jinlong Wang,Xing Cheng,Yue Tang,Lian Xia,Xiaojuan Niu,Yuangen Wu
Nanozyme-based sensors for detecting ascorbic acid (AA) generally depend on the reducibility of the analyte. However, these sensors are susceptible to interference from reducing substances in food. Herein, a novel fluorescent sensor for AA detection was developed based on inhibiting the phosphatase-like activity of a Zr-based metal-organic framework (Zr-CAU-28). Hydroxyl-rich AA molecules adsorb on the surface of Zr-CAU-28 through hydrogen bonding with [Zr 6 O 4 (OH) 4 ] cluster, leading to a decrease in the relative content of terminal hydroxyl groups within the catalytic sites. The constructed sensor exhibits a wide detection range (0.08–11 μg·mL −1 ) and low detection limit of 0.025 μg·mL −1 . Potential interfering studies demonstrated the good selectivity of the sensor. Moreover, the fluorescent sensor can effectively detect AA in juices and vitamin C tablets, with the recovery rate ranging from 96.25 % to 108.50 %. This work represents the first application of phosphatase mimics for AA detection, offering a new strategy for food analysis.
研究领域:
食品科学
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分析化学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
Molecular Medicine Reports
·
2016
·Vol. 15
·期 1
·387-395
·
IF 1.559
Bing Wang,Rui‑Ning Yang,Yue‑Rong Zhu,Ji‑Cheng Xing,Xiao‑Wei Lou,Yu‑Jie He,Qi‑Long Ding,Ming‑Yue Zhang,Hong Qiu
Xanthine oxidase (XOD) and paraoxonase 1 (PON1) are important enzymes in redox reactions in vivo, and are predominantly synthesized by the liver. The aim of the present study was to investigate the redox state in nonalcoholic fatty liver disease, and determine the association between the activities of XOD and PON1 and the severity of NAFLD. Sprague‑Dawley rats were randomly divided into control, model and α‑lipoic acid (high and low dose) groups. The rats in the NAFLD model were induced by feeding a high fat diet for 12 weeks and the in vitro cell model of hepatocyte steatosis was induced by treating L‑02 cells with oleic acid for 24 h. The body weight, liver function, lipid and oxidative stress indices, and histological features of the liver were examined in the rats. Compared with the control group, the rats in the NAFLD model group showed impaired liver function, lipid disorders and damage from oxidative stress. The serum activity of XOD increased significantly from the 4th week and was markedly higher, compared with that in the control group, reaching a peak in the 12th week. The activity of PON1 was negatively correlated with that of XOD. Compared with the control cells, the activity of XOD and levels of free‑fatty acids were significantly higher, and the activity of PON1 was significantly lower in the NAFLD L‑02 cell model. All the above indicators were significantly improved by treatment with the antioxidant, α‑lipoic acid. The activities of XOD and PON1 may be promising as markers in a noninvasive approach for detecting the severity of NAFLD clinically. α‑lipoic acid had protective effects on the NAFLD rats, and the potential mechanism may be associated with the inhibition of oxidative stress and lipid peroxidation.
研究领域:
肝病学
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生物化学
·
药理学
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产品关联
DOI
PubMed
高影响因子
Biomaterials Science
·
2017
·Vol. 5
·期 8
·1558-1566
·
IF 4.21
Shao-Lu Li,Yingqin Hou,Yali Hu,Jin Yu,Wei Wei,Hua Lu
We describe here the synthesis and cell-selective delivery of a cationic Pt(IV)-backboned prodrug-like polymer P(DSP-DAEP). P(DSP-DAEP) features excellent aqueous solubility, unusually high (44.5%) drug loading, can be rapidly reduced to release the active cisplatin, and is more potent than its small molecular Pt(IV) precursor DSP. P(DSP-DAEP) can be formulated with an oppositely charged methoxyl poly(ethylene glycol)-block-poly(L-phosphotyrosine) (mPEG-b-PpY) to afford a polyion micelle (Pt-PIC) by taking advantage of polyelectrolyte coacervation. Preliminary in vitro cellular uptake and cytotoxicity assays indicate that Pt-PIC exhibits receptor (surface alkaline phosphatase)-dependent uptake and cytotoxicity. Overall, our results suggest a new approach to the improved therapeutic index of platinum-based anticancer drugs via cell-selective delivery.
研究领域:
药物化学
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肿瘤学
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生物材料学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ALZHEIMERS DISEASE
·
2019
·Vol. 67
·期 4
·1353-1365
·
IF 3.517
Aluganti Narasimhulu Chandrakala,Mitra Connie,Bhardwaj Deepshikha,Burge Kathryn Young,Parthasarathy Sampath
Background:Alzheimer’s disease (AD) is a neurodegenerative disorder associated with aging. Cardiovascular risk factors like hypertension and atherosclerosis increase the risk for AD. Polymorphic alleles of apolipoprotein E (ApoE) are one of the main genetic determinants of AD. Objective:Mice, double-knockout (DKO) for ApoE (major cholesterol carrier in brain) and PON1 (paroxonase1, reduces oxidative stress), showed severe age-dependent atherosclerosis of the arteries carrying blood to the brain even on normal diet. This prompted us to investigate the possibility of an AD pathology resulting from the deficiency of ApoE and the induction of oxidative stress. Methods:Atherosclerotic lesions were quantified by ImageJ. The brain hippocampus of young and old ApoE-PON1 DKO mice and control mice were harvested. RT-PCR analysis was performed for mRNA levels of AD specific markers. Blood levels of S100 calcium-binding protein B (S100B) protein were measured by ELISA. H&E as well as immunostaining was performed to detect amyloid-β (Aβ) plaques and neurofibrillary tangles (NFTs) in brain tissues. Evans blue dye was used to evaluate the vascular permeability and blood-brain barrier (BBB) dysfunction. Results:Results showed that the older DKO mice had severe carotid atherosclerosis, increased mRNA levels of AD markers in brain tissue, and elevated levels of serum S100B protein. Immunological staining confirmed the characteristics of AD. Ex-vivo imaging showed higher levels of Evans blue dye in the ApoE-PON1 DKO mice brain tissues, pointing toward impaired vasculature. Conclusion:Aged ApoE-PON1 DKO mice displaying AD specific markers along with Aβ plaques, NFTs, and disrupted BBB suggests the animals are suffering from AD.
研究领域:
神经退行性疾病
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分子生物学
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病理学
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心血管研究
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DOI
PubMed
高影响因子
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