SEPARATION AND PURIFICATION TECHNOLOGY
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2026
·
IF 9
Bo Xu,Zhengcai Zhu,Silin Yang,Haiyan Yang,Jing Yang,Zhengjun Shi,Dawei Wang
Regulating the surface charge of biochar is crucial for its catalytic performance and adsorption capability. Here, Cu 2+ and melamine (Me) modified pomegranate peel powder (Cu-Me-PP) was prepared by coordination self-assembly method using waste pomegranate peel as the raw material. Then, nano-CuO and N-graphite doped biochar (CuO-N-PC) with positively charged surfaces were successfully prepared by pyrolysis of Cu-Me-PP and valence state regulation. The CuO-N-PC-300/ peroxymonosulfate (PMS) system could completely degrade the chloroquine phosphate (CQ) antibiotic within 30 min. Moreover, the effects of catalyst dosage, PMS dosage, solution pH, CQ concentration, and coexisting ions on the degradation efficiency of CQ were studied. It was found that CuO-N-PC-300 could not only catalyze the degradation of CQ but also remove 86.8 % of the phosphorus in the solution via electrostatic adsorption. Zeta potential analyses and molecular electrostatic potential calculations revealed that the electrostatic adsorption between the positively charged biochar and PO 4 3− were the key factors for the recovery of phosphorus. Electrochemical analyses, quenching experiments, electron paramagnetic resonance (EPR) tests, and DFT calculations were employed to explore the potential activation mechanism of PMS. Both interfacial electron transfer and the Cu 2+ /Cu + redox reaction were important pathways for activating PMS, generating a large number of •OH and 1 O 2 during the degradation of CQ. Finally, two degradation pathways of CQ were proposed based on LC-MS analysis, and the ecological toxicity was evaluated. This work provided an efficient method for the activation of PMS and the recovery of phosphorus by constructing positively charged nano-CuO sites on the surface of biochar.
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JOURNAL OF MEMBRANE SCIENCE
·
2026
·
IF 8.8
Meng You,Chao Xia,Bo Jin,Qianlong Sun,Bingbing Yuan,Ping Hu,Xinru Hu,Siyi Wang,Jianqiang Meng
Developing nanofiltration (NF) membranes that simultaneously achieve high water permeance and selectivity remains a fundamental challenge due to their inherent permeability-selectivity trade-off. Herein, we report a monomer diffusion regulation strategy using amphiphilic sulfonated resorcinarenes (SRA) to fabricate high-performance polyesteramide NF membranes. Incorporated SRAs lower interfacial tension, facilitate PIP diffusion, and self-assemble into physical template aggregates. Driven by the kinetic competition between SRA hydroxyl groups and PIP amines for TMC stemming from their reactivity difference, SRAs temporarily retard crosslinking at SRA-rich sites while their amphiphilicity directs PIP toward adjacent regions to resume polymerization. This kinetic competition, combined with post-encapsulation collapse of SRA assemblies, yields polyesteramide membranes with characteristic crater-like structures and abundant nanovoids within the selective layer. These features increase the effective permeable area and reduce mass transfer resistance. The optimized membrane delivers a water permeance of 26.3 L m −2 h −1 bar −1 , with Na 2 SO 4 rejection of 98.87%, and a Cl − /SO 4 2− selectivity factor of 409.4 in mixed salt feed (1000 ppm Na 2 SO 4 +1000 ppm NaCl). SRA-PIP/TMC membranes achieve improved rejection toward organic micropollutants (OMPs), especially negatively charged hydrophobic species such as ofloxacin and chloroquine phosphate, through the synergistic effects of size sieving, Donnan repulsion, and host-guest inclusion within resorcinarene cavities. Benefiting from ester linkages, the membranes possess good chlorine resistance. This work demonstrates the potential of amphiphilic macrocycle co-monomers for regulating polymerization toward the preparation of high-performance NF membranes for water purification and precise molecular separation.
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JOURNAL OF PROTEOME RESEARCH
·
2026
·
IF 3.8
Chen Xiangling,Huang Nan,Xu Zhihan,Luo Juan,Dong Qiongye,Lyu Zhaojie
Clear cell renal
cell carcinoma (ccRCC) is the predominant
histological
subtype of kidney cancer, characterized by a paucity of therapeutic
targets and a dismal prognosis in advanced stages. Although family
with sequence similarity 49 member B (FAM49B) has been implicated
in tumor progression with context-dependent roles, its function in
ccRCC remains undefined. Herein, we elucidated the expression profile
and regulatory mechanisms of FAM49B by utilizing integrated bioinformatics,
molecular biology, and proteomic approaches. Analysis of TCGA and
CPTAC data sets revealed a significantly upregulated FAM49B protein
in ccRCC tissues that positively correlated with pathological grade,
while the mRNA level was unchanged. Mechanistically, FAM49B undergoes
proteasomal degradation, whereas the deubiquitinating enzyme USP9X
directly binds to and stabilizes FAM49B. Pharmacological inhibition
or knockdown of USP9X notably reduces FAM49B protein abundance without
altering its transcription. Quantitative proteomic and affinity mass
spectrometry identified ATP1B1 as a critical downstream effector of
FAM49B. Western blotting (WB) analysis verified that silencing FAM49B
downregulated ATP1B1 in ccRCC cells, while coimmunoprecipitation assays
established their direct physical interaction. Consistent with FAM49B
expression profiles, ATP1B1 protein levels were elevated in high-grade
ccRCC specimens. Collectively, these findings delineate a USP9X–FAM49B–ATP1B1
signaling axis that drives ccRCC progression, representing a promising
diagnostic biomarker and potential therapeutic target.
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高影响因子
EXPERIMENTAL EYE RESEARCH
·
2026
·
IF 3.1
Yan Peng,Bo Xiao,Feiyu Wang,Xiaokun Shen,Jinwen Huang,Jinghua Yu,Xingxing Diao
First report on mefuparib's ocular accumulation via melanin binding. • Mefuparib accumulation in ocular pigment membrane via QWBA. • High melanin affinity drives ocular retention of mefuparib. • Mefuparib shows 58-fold higher eye exposure than M8 in LE rats. Mefuparib, a novel poly (ADP-ribose) polymerase inhibitor (PARP), is primarily utilized for treating various advanced or metastatic malignant tumors. Previous research has indicated that mefuparib-related compounds accumulate notably in the eyes. However, the precise mechanism and components of this accumulation remain unclear. Using quantitative whole-body autoradiography (QWBA), cell uptake and release models, and in vitro melanin binding experiments to explore the accumulation characteristics of mefuparib and its N-dealkylation metabolite M8 in the eye. Results showed the area under the curve (AUC 0-48h ) of mefuparib in Long-Evans (LE) rats was 58.1 times higher than in Sprague-Dawley (SD) rats, with a longer half-life. Conversely, M8 had a lower (AUC 0-48h ) in LE rats. This indicates the main substance accumulated in the eyes is mefuparib, and the accumulation site is the pigment membrane of LE rats. The uptake of mefuparib by Retinal Pigment Epithelium (RPE) cells was 2.81-4.92 times that of Adult Retinal Pigment Epithelial-19 (ARPE-19) cells, and the release within 24 h was only 30.5%. Its melanin binding parameter B max (451 ± 3.15 nmol/mg) was significantly higher than that of M8 (120 ± 6.43 nmol/mg). In conclusion, the high uptake, low release and strong melanin binding capacity of mefuparib jointly lead to its intraocular accumulation, providing data support for the subsequent optimization of compound structure to reduce ocular accumulation or offering new directions for the development of new ocular treatments. Download: Download high-res image (235KB) Download: Download full-size image
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CHEMICO-BIOLOGICAL INTERACTIONS
·
2026
·
IF 5.2
Jiaqi Li,Zhengxin Lu,Nan Zhang,Xiaowei Zhou,Yuanbiao Ma,Jia-qi Li,Yimu Zhang,Zhewen Deng,Na Lin,Peng Yue,Yilin Yang,Qi Zhang,Bo Ma
Andrographolide (Andro), the primary bioactive compound of Andrographis paniculata , has known bioactivities but also male reproductive toxicity with unclear mechanisms. This study investigated its testicular injury effects and mechanism, focusing on Sertoli cells (SCs) ferroptosis. Toxicity was evaluated in mice and SCs. Mechanisms were probed using RT-qPCR, Western blot, co-immunoprecipitation, and ubiquitination assays. LC-MS/MS assessed tissue distribution. Molecular docking and dynamics simulations characterized the Andro-β-TrCP interaction. The results demonstrated that Andro exposure caused significant testicular atrophy, blood-testis barrier (BTB) disruption, and spermatogenic impairment in mice. Meanwhile, pharmacokinetic analysis revealed SCs as the primary target of Andro-induced testicular damage, showing rapid Andro accumulation that was subsequently detected in sperm and the epididymis. Furthermore, Andro triggered ferroptosis in testicular injury and SCs, marked by increased ROS, MDA, iron overload, and lipid peroxidation. These effects were rescued by the ferroptosis inhibitor deferoxamine. Mechanistically, Andro directly bound to β-TrCP, downregulating its expression and subsequently stabilizing ATF4 by impairing its ubiquitin-mediated degradation. Silencing ATF4 inhibited ferroptosis and restored cell viability upon Andro challenge. The direct Andro-β-TrCP interaction was consistently confirmed by molecular docking, dynamics simulations, and CETSA. This study reveals that Andro triggers SCs ferroptosis by inhibiting β-TrCP-mediated ATF4 ubiquitination and degradation, leading to BTB disruption and spermatogenic impairment. These findings provide a theoretical basis for optimizing clinical application of Andro in the future.
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BIOMACROMOLECULES
·
2025
·
IF 5.4
Chunfa Chen,Cheng Tian,Zhuoer Jin,Yuandong Wen,Xiaoyu Xia,Qiao Ren,Cheng Zhi Huang,Hua Zuo
TLR9 agonists hold significant potential in vaccine development and cancer immunotherapy. While synthetic CpG oligodeoxynucleotides (ODNs) have been employed in immunotherapies, their clinical application remains constrained by dose-limiting toxicity and metabolic instability. DNA nanotechnology provides a promising strategy for the precise modulation of ligand-TLR9 interactions. In this study, DNA nanostructure-based TLR9 agonists were rationally designed by optimizing the spatial orientation of 5′-TCG ligands based on the two DNA-binding sites revealed in the TLR9 receptor crystal structure. Fine-tuning DNA nanostructure configurations enabled controllable modulation of TLR9 activation with structural flexibility identified as a critical determinant. Notably, the oligomerization of DNA nanostructures markedly enhanced TLR9 stimulation efficacy, establishing a paradigm for the rational design of TLR9 agonists. These findings advance innovative approaches for developing next-generation immunotherapeutic agents.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Jing Wang,Ziyang Chu,Haibo Liu,Zhengyan Han,Tianhu Chen,Xuehua Zou,Dong Chen
Advanced oxidation processes employing natural carbonate minerals to activate peroxymonosulfate (PMS) have exhibited remarkable efficacy in the removal of organic pollutants. In this study, the differences in the degradation performance and mechanism of chloroquine phosphate (CQP) by calcite/PMS and dolomite/PMS systems were investigated. Experimental findings reveal that the calcite/PMS system demonstrates markedly superior removal under identical conditions, achieving 99 % CQP removal within 60 min. In contrast, the dolomite/PMS system only achieved 63 % CQP removal under the same conditions. Electron paramagnetic resonance (EPR) and radical quenching analyses identified singlet oxygen ( 1 O 2 ) as the dominant reactive species in both systems. Quantitative assessments indicated that the calcite/PMS system generated more 1 O 2 (43.3 μM) compared to the dolomite/PMS system (35.4 μM.). This phenomenon was attributed to that calcite released more carbonate ions (CO 3 2− ) than dolomite. Although the two systems had different reaction rates, both achieved stable CQP removal over 90 % in a one-month continuous flow column experiments. This work developed the application potential of carbonate minerals in advanced oxidation systems. Furthermore, the findings contribute to better understanding and development of carbonate activated persulfate systems.
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iScience
·
2025
·
IF 4.1
Jing Mo,Qiling Cai,Shanshan Chen,Jinlan Luo,Zhibiao Hu,Lili Su,Lulu Cheng,Lijie Huang,Shijia Liu,Xiangru Wang,Qinying Liu,Li Chen,Shuichun Mao,Yang Sun
A growing body of research suggests that inhibition of autophagy may be a novel means of treating cancer and suppressing drug resistance. Therefore, a series of drugs derived from the Erythrina crista-galli Linn were screened in this study. Among them, the pterocarpan erythrabyssin II (EL-19) is a potent late-stage autophagy inhibitor, which could effectively block the fusion of autophagosome and lysosome, leading to the accumulation of autophagic substrates in both ovarian cancer A2780 and A2780/DDP cells. EL-19 did not impair the lysosomal pH and lysosomal enzyme activity. In addition, cell studies, and organoid experiments showed that EL-19 inhibited the value addition of A2780 and A2780/DDP cells, suppressed ovarian cancer organoid activity and induced apoptosis, and blocked cisplatin-induced protective autophagy in A2780/DDP cells. Combination therapy with DDP superior anti-tumor outcomes compared to monotherapies in animal models. In summary, EL-19 may be developed as an anticancer agent by blocking chemotherapy-induced protective autophagy.
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BIOGERONTOLOGY
·
2026
·Vol. 27
·期 2
·72
·
IF 5.3
Zhang Xiaoli,Wang Chengniu,Chen Weiguan
Aging is a major risk factor for the onset and progression of many neurodegenerative diseases. Inflammation is the body’s natural defense mechanism against harmful stimuli . A growing body of research has highlighted the intricate relationship between aging and inflammation, with chronic low - grade inflammation, often referred to as “inflammaging,” being a hallmark of the aging process. Cycloneolitsol (CL) is a C-32 cycloartane-type triterpene from Taxodium ascendens which has been reported to possess the anticancer, neuroprotection and antibacterial activities. Its specific role and the underlying molecular mechanisms in modulating the aging process remain unclear. Our study aimed to explore the possible anti-aging effect and mechanisms of CL. Network pharmacology predicted core targets and several potential pathways, which are associated with the anti-aging effect of CL. RAW264.7 macrophages and Caenorhabditis elegans were selected for evaluating its anti-aging and anti-inflammation activity. The results showed that CL exerted obvious anti-inflammatory effects against LPS-induced M1-type polarization of RAW264.7 cells via NF-κB signaling pathway. In C. elegans , CL improved Biological Characteristics and prolonged the lifespan without reproductive toxicity. Furthermore, experiments on different mutant C. elegans strains and the nuclear translocation of SKN-1 confirmed that CL activates autophagy and enhances the antioxidant stress response ability through the SEK-1/PMK-1/SKN-1 signaling pathway. This study provides novel insights into the biological activities of CL, highlighting its potential as a natural product for treating inflammation-related diseases and delay aging.
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Materials Today Advances
·
2026
·
IF 8
Ling Zhang,Yongsheng Du,Rui Gao,Yu Dong,Miaomiao Zhang,Qiaoyun Wang,Fei Tong,Weiwei Qiao
Autophagy was associated with the pathophysiology of various diseases in vivo , and its own regulation was beneficial for the outcomes of specific diseases. Here, we successfully designed a biocompatible active gliding-nanomachine for delivery of chloroquine (CQ)/nanodiamond (ND) and accelerated tumor cell death associated with amplifying ferroptosis and declining autophagy in vitro . Fe was covered onto one side of the surface of the L-arg@CQ/ND@HA, functioning as a controller for the dominated release of NO by sensitive ROS and Fenton-reaction from the catalyzation between Fe 2+ and H 2 O 2 in vitro . The asymmetrical NO release, which was not only the active-component to decline tumor autophagy in combination with ND/CQ, but also the propellants for the nanomachines. After implementing in tumor cell, the dynamic-sliding of nanomachines could be triggered via ROS and the proactive-gliding further strengthened diffusion of CQ/ND, leading to precise-treatment of tumors through enlarging ferroptosis accompanied by polyamine output and interruption of autophagosome lysosome fusion. The synthetized dynamic sliding-nanomachines, with ROS stimulation of movement behavior and proactive delivery of CQ/ND unlocked huge latent capacity as a potential platform for tumor treatment stemed from ferroptosis amplification loop induced polyamine output and declining autophagy in tumor cell.
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Frontiers in Pharmacology
·
2022
·
IF 5.988
Wang Junqi,Jing Xian,Hua Lizhong,Zheng Yuling,Hu Shiheng,Xiao Jing,Guo Dawei,Wu Wenda,Ji Hui,Peng Lin,Jiang Shanxiang,Gao Xiuge
Chloroquine was once thought to be a promising treatment for COVID-19 but it quickly failed due to its inefficiency and association with increased mortality. Further, comorbidities such as hypertension may have contributed this failure. The safety and toxicity of chloroquine at doses required for treating SARS-CoV-2 infection in hypertensive patients remain unknown. Herein, to investigate these effects, we performed a safety evaluation of chloroquine at the approved dose (63 mg/kg) and at a high dose (126 mg/kg) in hypertensive rats. We found that chloroquine increased the mortality of hypertensive rats to 18.2% and 100%, respectively, after 7 days. During the chloroquine exposure period, the bodyweight, feed, and water consumption of hypertensive rats were decreased significantly. In addition, we show that chloroquine induces prolongation of QTc interval, elevation of LDH and CK, and histopathological damage of the myocardium in hypertensive rats. Ocular toxicity was observed in hypertensive rats in the form of hemorrhage in the eyes and retinal damage. Furthermore, we also observed intestinal toxicity in hypertensive rats, which presented as thinning intestinal walls with hemorrhagic contents, and histopathological changes of the jejunum. Hepatotoxicity was also evidenced by elevated ALT, and vacuolization of hepatocytes was also observed. Nephrotoxicity was observed only in high dose chloroquine-treated hypertensive rats, presenting as alterations of urinalysis and renal function. Immune alterations were also found in high-dose chloroquine-treated hypertensive rats with elevation of serum IL-10, IL-1β and GRO, and moderate damage to the spleen. In summary, this study partially explains the reason for the failure of chloroquine as a COVID-19 therapy, and underlines the importance of safety evaluation and medical supervision of chloroquine to avoid patient harm, especially to those with hypertension.
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ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Xinqi Zheng,Pengran Guo,Yue Zhang,Jingwei Xu,Jian Sun,Yongqian Lei
The environmental pollution and human health risks caused by anti-infective residual drugs in the environment have attracted much attention. More convenient and effective detection methods to achieve the rapid and high sensitivity detection for such pollutants are required. In this work, a novel surface-enhanced Raman scattering (SERS) strategy based on vortex aggregation of AgNPs was proposed for the detection of anti-infective drugs in environmental water. The method enhanced the Raman signal of the targets by 2–7.4 times. The mechanism of aggregation enhancement effect under the low-frequency oscillation procedure which significantly enhanced the SERS signal of targets molecular on the aggregated AgNPs was revealed by UV–vis and ICP-MS methods. Three drugs of cefazolin sodium, pefloxacin , and chloroquine phosphate were determined. The detect limits were 3.97 × 10 −9 mol/L, 2.42 × 10 −10 mol/L, and 7.34 × 10 −9 mol/L for cefazolin sodium, pefloxacin, and chloroquine phosphate, respectively. The quantitative relationships were obtained in a wide linear range of 4–5 orders as well as good accuracy and stability with the recoveries of 84.0%–97.1% and the relative standard deviations (RSDs) less than 4.6% for spiked in actual water samples. This method also had excellent repeatability and stability, which have potential application for rapid detection of trace pollutants in water environment.
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危险品化学品经营许可证（带存储）