Journal of Environmental Chemical Engineering
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2026
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IF 7.5
Meng Zhang,Xuewei Li,Jiuyang Lin,Yinhua Wan,Weijie Song
Magnesium sulfate is widely used as a leaching agent in ionic adsorption rare earth (RE) ore mining, but it produces leachates containing high concentrations of Mg²⁺ and Ca²⁺ and only trace levels of rare earth ions (RE³⁺), which leads to low-purity RE products and difficult enrichment. Here, we adopt an extraction-precipitation strategy using recyclable complexing agents—glyphosate (Gly), hydroxyethylidene diphosphonate (HEDP), creatine phosphate (CP), and ethylenediamine tetramethylenephosphonate (EDTMP)—to concentrate dilute RE₂(SO₄)₃ and further obtain a concentrated RECl₃ product. The results showed that at pH 3.5, with RE: CP molar ratio of 1:2, CP achieved higher precipitation efficiency for RE³⁺ than Gly, HEDP, and EDTMP. CP enabled the selective separation of RE 3+ (La, Nd, Y, Yb) from Mg²⁺ and Ca²⁺, enriching RE to 198.73 g·L⁻¹ in a single step, which is 933.65 times higher than that in the original solution. Meanwhile, the purity of the RE products reached 99.33%. This outstanding performance was further validated using actual mine leaching solutions, where the RE³⁺ concentration reached 261.57 g·L⁻¹. In addition, CP can be effectively recovered and reused. Overall, this method provides a simple, efficient, and environmentally friendly approach for high-purity RE recovery from impurity-rich leachates.
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ACS Applied Materials & Interfaces
·
2022
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IF 10.383
Yingqi Chen,Weibei Sheng,Jianjing Lin,Chongzhou Fang,Jiapeng Deng,Peng Zhang,Meng Zhou,Peng Liu,Jian Weng,Fei Yu,Deli Wang,Bin Kang,Hui Zeng
Natural polysaccharide (NPH)-based injectable hydrogels have shown great potential for critical-sized bone defect repair. However, their osteogenic, angiogenic, and mechanical properties are insufficient. Here, MgO nanoparticles (NPs) were incorporated into a newly synthesized water-soluble phosphocreatine-functionalized chitosan (CSMP) water solution to form an injectable hydrogel (CSMP-MgO) via supramolecular combination between phosphate groups in CSMP and magnesium in MgO NPs to circumvent these drawbacks of chitosan-based injectable hydrogels. Water-soluble chitosan deviate CSMP was first synthesized by grafting methacrylic anhydride and phosphocreatine into a chitosan chain in a one-step lyophilization process. The phosphocreatine in this hydrogel not only provides sites to combine with MgO NPs to form supramolecular binding but also serves as the reservoir to control Mg2+ release. As a result, the lyophilized CSMP-MgO hydrogels presented a porous structure with some small holes in the pore wall, and the pore diameters ranged from 50 to 100 μm. The CSMP-MgO injectable hydrogels were restricted from swelling in DI water (lowest swelling ratio was 16.0 ± 1.1 g/g) and presented no brittle failure during compression even at a strain above 85% (maximum compressive strength was 195.0 kPa) versus the control groups (28.0 and 41.3 kPa for CSMP and CSMP-MgO (0.5) hydrogels), with regulated Mg2+ release in a stable and sustained manner. The CSMP-MgO injectable hydrogels promoted in vitro calcium phosphate (hydroxyapatite (HA) and tetracalcium phosphate (TTCP)) deposition in supersaturated calcium phosphate solution and presented no cytotoxicity to MC3T3-E1 cells; the CSMP-MgO hydrogel promoted MC3T3-E1 cell osteogenic differentiation with upregulation of BSP, OPN, and Osterix osteogenic gene expression and mineralization and HUVEC tube formation. Among them, CSMP-MgO (5) presented most of these properties. Moreover, this hydrogel (CSMP-MgO (5)) showed an excellent ability to promote new bone formation in critical-sized calvarial defects in rats. Thus, the CSMP-MgO injectable hydrogel shows great promise for bone regeneration.
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Applied Materials Today
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2018
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IF 0
Lei Liu,Yingcong He,Xuetao Shi,Huichang Gao,Yingjun Wang,Zhengmei Lin
By mimicking the role of phosphate groups in native tissues and encouraging tissue formation, phosphate-functionalized biopolymers have been widely employed to accelerate the formation of bone injury. In this study, phosphocreatine is screened as a phosphate source and applied in the preparation of phosphocreatine-modified chitosan (CS-P) and porous scaffolds. CS-P exhibits much better hydrophilicity, solubility, protein adsorption and mineralization than pure chitosan (CS). Meanwhile, in comparison to CS scaffold, the CS-P scaffold significantly enhances the in vitro osteogenic differentiation of hFOB cells, indicated by the higher alkaline phosphatase (ALP) activity, denser mineralization deposition and up-regulated osteogenesis-related genes including runt-related transcription factor-2, ALP, osteocalcin and osteopontin at both mRNA and protein levels. The in vivo results indicate an enhancing effect of the CS-HP scaffold on the bone regeneration ability. We further confirm that phosphocreatine modification promotes the osteogenic differentiation of hFOB cells by activating the MAPK signalling pathway. The phosphocreatine-modified chitosan holds great potential for bone repair and regeneration.
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-22
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IF 15.7
Wang Ziyi,Ge Siyuan,Liao Tiepeng,Yuan Man,Qian Wenwei,Chen Qi,Liang Wei,Cheng Xiawei,Zhou Qinghua,Ju Zhenyu,Zhu Hongying,Xiong Wei
Emerging evidence has unveiled heterogeneity in phenotypic and transcriptional alterations at the single-cell level during oxidative stress and senescence. Despite the pivotal roles of cellular metabolism, a comprehensive elucidation of metabolomic heterogeneity in cells and its connection with cellular oxidative and senescent status remains elusive. By integrating single-cell live imaging with mass spectrometry (SCLIMS), we establish a cross-modality technique capturing both metabolome and oxidative level in individual cells. The SCLIMS demonstrates substantial metabolomic heterogeneity among cells with diverse oxidative levels. Furthermore, the single-cell metabolome predicted heterogeneous states of cells. Remarkably, the pre-existing metabolomic heterogeneity determines the divergent cellular fate upon oxidative insult. Supplementation of key metabolites screened by SCLIMS resulted in a reduction in cellular oxidative levels and an extension of C. elegans lifespan. Altogether, SCLIMS represents a potent tool for integrative metabolomics and phenotypic profiling at the single-cell level, offering innovative approaches to investigate metabolic heterogeneity in cellular processes.
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ACS Biomaterials Science & Engineering
·
2024
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IF 5.4
Yixiao Chen,Guoqing Li,Yufeng Ge,Su Liu,Jian Weng,Jianjing Lin,Ao Xiong,Hui Zeng,Xinbao Wu,Jun Yang,Fei Yu
In this study, we explore the cartilage defect repair mechanism by phosphocreatine-grafted chitosan hydrogels loaded with berberine-treated ATDC5 cells (CSMP@BBR@ATDC5). Under the optimal concentrations of LPS and BBR ideal conditions, ATDC5 cell toxicity and proliferation were detected with AM/PI and EdU staining. Additionally, qPCR and Western blot were employed to detect the expression of the SIRT1/BMP4 signaling pathway and chondrogenic-related factors in ATDC5 cells. Moreover, BBR-treated ATDC5 was seeded into a phosphocreatine-grafted chitosan hydrogel system. Subsequently, the cartilage defect was established in mice. After 4, 8, and 12 weeks, knee specimens were collected to evaluate the repair of cartilage defects. According to our findings, BBR can increase ATDC5 viability by LPS treatment. Likewise, it upregulates the SIRT1/BMP4 signaling pathway expression and chondrogenic-related factors. Another, it was shown by histological observation that the cartilage defect had been repaired more effectively in the CSMP@BBR@ATDC5 group than in the other groups. Finally, the expressions of chondrogenic-related factors and SIRT1/BMP4 signaling pathway were upregulates in CSMP@BBR@ATDC5 than in other groups. In vitro, BBR protects inflammatory ATDC5 cells and maintains the expression of chondrogenic-related factors. Subsequently, we successfully use CSMP@BBR@ATDC 5 to repair knee cartilage defects in mice.
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CHEMICAL ENGINEERING JOURNAL
·
2025
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IF 13.2
Haotian Qin,Peng Zhang,Jin Zhao,Zhenhai Xie,Sen Yao,Tiantian Qi,Weibei Sheng,Deli Wang,Yingqi Chen,Junyu Qian,Hui Zeng
Repairing critical-sized bone defects remains a significant clinical challenge. Although chitosan (CS)-based hydrogels show promise for bone regeneration, their application is limited by insufficient mechanical strength and inadequate osteogenic and angiogenic properties. Herein, we synthesized Zn- and Sr-containing phosphate particles (ZnSrP) via a sintering and ball milling process, followed by surface modification with polydopamine (PDA) to obtain bioactive nanoparticles. These PDA-coated ZnSrP nanoparticles were incorporated into a phosphocreatine-grafted, photo-crosslinked methacryloyl chitosan (CSMP) hydrogel to form a CSMP&ZnSrP composite system. The resulting hydrogel exhibited enhanced compressive strength and modulus, and enabled sustained release of Zn 2+ and Sr 2+ ions. Mechanistically, Zn 2+ and Sr 2+ promoted angiogenesis in HUVECs via activation of the HIF-1α pathway and osteogenesis in BMSCs through the PI3K/Akt/Sgk1 signaling axis. Notably, the CSMP&ZnSrP hydrogel synchronized the coupling of angiogenesis and osteogenesis between HUVECs and BMSCs. In both the rat calvarial and rabbit femoral condyle critical-sized defect models, the hydrogel significantly accelerated new bone formation, underscoring its potential as an advanced injectable material for critical-sized bone regeneration.
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高影响因子
Materials Today Bio
·
2025
·
IF 10.2
Haotian Qin,Zhiping Guan,Yuanhao Wang,Jin Zhao,Zhenhai Xie,Huaiyu Li,Chen Zhang,Weibei Sheng,Fei Yu,Jian Weng,Yingqi Chen,Deli Wang,Hui Zeng,Junyu Qian
Osteoporotic bone defects remain a major clinical challenge due to impaired osteogenesis, insufficient angiogenesis, excessive osteoclast activity, and increased susceptibility to infection. To address these issues, we developed an injectable phosphocreatine-grafted gelatin hydrogel (GGP) incorporating hierarchically structured Zn-Cu particles functionalized with a teriparatide (PTH)/strontium–zinc phosphate (SrZnP) hybrid coating. This multifunctional hydrogel was fabricated via enzymatic and ionic coordination crosslinking, yielding improved mechanical properties and sustained release of Zn 2+ , Sr 2+ , and PTH. In vitro evaluations demonstrated that the hydrogel enhanced BMSC proliferation, osteogenic differentiation, and mineralization, promoted HUVEC migration, tube formation, and angiogenic marker expression, and simultaneously inhibited osteoclastogenesis and bacterial growth. Transcriptomic analysis and inhibitor experiments revealed a dual paracrine mechanism mediating bone–vascular coupling: BMSC-derived HIF-1α–VEGF signaling facilitated angiogenesis, while HUVEC-derived PI3K–Akt–BMP-2 signaling enhanced osteogenesis. In vivo, the PTH/SrZnP@ZnCu-GGP hydrogel significantly accelerated bone regeneration and neovascularization in an ovariectomized rat calvarial defect model, accompanied by upregulated expression of BMP-2, RUNX2, p-Akt, and CD31. Collectively, this injectable hydrogel system offers a robust and translationally feasible strategy for coordinated osteogenesis–angiogenesis coupling, osteoclast suppression, and antibacterial defense, thus holding strong potential for the regeneration of osteoporotic bone defects.
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高影响因子
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