FOOD CHEMISTRY
·
2020
·
IF 6.306
Jiaxin Wang,Xuening Lu,Jiaen Zhang,Zeheng Xiao
Lipids such as phosphatidylcholine (PC), 3- sn -phosphatidylethanolamine (PE), sphingomyelin (SM) and L-α-lysophosphatidylcholine (LPC) are the major components of biological membranes and play important roles in physiological functions. Here, PC, PE, SM, and LPC were extracted from golden apple snails (GAS, Pomacea canaliculata ) and GAS flesh (GASF) using an ethanol/hexane sequential scheme and quantified simultaneously using ultra-high performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UHPLC-ESI-MS/MS) to evaluate whether the GAS could be the source of the four lipids. Our results suggest that ethanol extracts contained the most crude lipids, and the yield of dry (evaporated) lipids were 3.45 g per 100 g fresh GASF and 1.82 g per 100 g of fresh GAS. Quantification of the lipids using UHPLC-ESI-MS/MS suggested that GAS contained PE, PC, SM and LPC, with SM being the most abundant lipid (after purification: 1.71 and 1.42 mg g −1 dry weight from 100 g of GASF and GAS, respectively). The method we used is cost-effective, and the recovery rates of ethanol and hexane ranged from 80−91% and 87−91% respectively. Overall, GAS and GASF are potential raw materials for lipids such as SM and PC extraction using the ethanol/hexane method. Comparatively, lipids extraction from the GAS is more effective and timesaving. Our finding would provide a way to utilize GAS and potentially control its invasion.
研究领域:
生物化学
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分析化学
·
入侵生物学
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DOI
高影响因子
JOURNAL OF SOILS AND SEDIMENTS
·
2021
·Vol. 21
·期 6
·2287-2300
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IF 3.308
Kang Longfei,Zhang Guoqiao,Chu Guixin
Purpose Phosphorus (P) fertilization methods significantly affect soil P availability, transformation, and crops yield. However, contradictory or paradoxical results are often available, and debates about this issue have never ceased so far. Therefore, the aims of this study are to explore influences of different P application methods on availability and transformation of added P in drip-irrigated system, and the responses of maize P uptake and its agronomic performances to P fertigation are also investigated. Materials and methods Here, we conducted a pot and filed experiments in drip-irrigated calcareous soil. Treatments included different P sources (phosphoric acid; polyphosphoric acid) with basal and split repeated application manners. Results and discussion Compared to the basal application, the average values of C DGT -P (P concentrations tested via diffusive gradient in thin films technique), Olsen-P, water-soluble P, and in situ P in the split repeated P application increased by 23.7%, 10.2%, 16.5%, and 34.4%, respectively (pot experiment). Furthermore, the average values of labile P (resin P and NaHCO 3 -P) and moderately labile P (NaOH-P) with the repeated P addition increased by 19.7% and 12.6%, whereas the concentrations of non-labile P lowered by 19.3%, respectively, relative to basal application treatment (pot experiment). The same trend was obtained in field experiment, implying that complete repeated P addition significantly increased P availability, reduced soil P transformation, and decreased P fixation. Therefore, a better agronomic performance of maize (P uptake, grain yield, and phosphorus use efficiency) was achieved in the complete repeated P application rather than basal application. Besides, diffusive gradient in thin film technique (DGT) was proved to be an accurate and powerful approach in testing soil available P. Conclusions Our findings elucidate that repeated delivering P through fertigation method can be recommended as an effective P application strategy in drip-irrigated field, especially in semi-arid and arid regions. The feasibility of DGT method was certified and C DGT -P could well characterize plant-accessible P.
研究领域:
农业
·
土壤学
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植物营养
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环境科学
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DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2022
·
IF 7.968
Runhao Zhang,Miao Gong,Yujie Yan,Jiangya Ma,Huiwen Zhang
In this study, the degradation behaviour of phospholipid 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) during hydrothermal conversion (HC) and the effect of alkali additives on phosphorus forms during HC of phospholipids and different phosphorus-containing wastes were investigated. The mechanism of phosphate-alkali-directed regulation and transformation was discussed and analysed with batch hydrothermal treatment experiments and characterizations such as XRD and SEM. The obtained results showed that DOPC degradation was divided into two periods, i.e., the initial accumulation of phosphorus-containing precursors with increasing temperature, followed by complete degradation to H 3 PO 4 as treatment continued. In addition, compared with the activation of P by NaOH, Ca(OH) 2 is more favourable for the stabilization of P and the migration of non-apatite inorganic phosphorus (NAIP) to apatite inorganic phosphorus (AP). The pH and appropriate feed Ca/P molar ratio during HC determine the degree to which total phosphorus is converted to Ca-bound P. The findings reveal the mechanism of phosphate-alkali-directed regulation and transformation and provide a theoretical reference for the efficient treatment of phosphorus-containing waste and the alkali-directed production of high-phosphorus bioavailability products.
研究领域:
环境科学
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生物化学
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产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2021
·Vol. 45
·期 42
·19876-19882
·
IF 3.591
Ling Zhao,Hanping Fu,Lishan Jian,Yating Zeng,Liran Liu,Qingshuang Liang,Xiufeng Xiao
In this work, we report a novel phosphorus element heterostructure with a snowflake-like morphology consisting of 1D rod-like black phosphorus (BP) and 2D flake-like red phosphorus (RP). The content of black phosphorus in the heterostructure can be adjusted and the light absorption range and photocatalytic performance of the heterostructure increase with the proportion of black phosphorus. In this 1D/2D phosphorus element heterostructure, the photogenerated electrons in the conduction band of BP can be used for water decomposition to produce hydrogen, while the photogenerated holes in the valence band of RP can be used to produce oxygen, without using any sacrificial agents. The ratio of hydrogen and oxygen evolution rates is 2 : 1, and the rates are 2.0 and 1.1 μmol g−1 h−1, respectively. Therefore, we believe that this work demonstrates the potential of metal-free elemental photocatalysts with high efficiency, especially in overall pure-water splitting.
研究领域:
材料科学
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化学
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Feifei Xu,Danyi Shang,Congcong Zhu,Guangzhu Du,Jingchen Shi,Xuefang Dong,Xiuling Li,Xinmiao Liang
Phosphopeptide enrichment methods based on commercial TiO2 suffer from difficulties in regulating intermolecular interactions, resulting in low coverage rate and the loss of information on multiphosphorylation sites, thereby limiting comprehensive phosphoproteomic analysis. In this work, MXene Ti3C2Tx was incorporated into the design of enrichment materials, with its surface structure functionalized and regulated to address the low elution efficiency of TiO2 for multiphosphorylated peptides. Upon oxidation treatment, the Ti3C2Tx material formed numerous uniformly distributed TiO2 nanoparticles on the surface of Ti3C2Tx-O, providing abundant affinity sites (Ti–O) for selective phosphopeptide enrichment. The polycrystalline structure and rich oxygen vacancies of the material effectively regulated its binding affinity with phosphate groups, achieving simultaneous high-efficiency enrichment of both monophosphorylated and multiphosphorylated peptides. Its performance was significantly superior to that of commercial TiO2 and IMAC materials. This study presents great promise for the practical application of comprehensive phosphoproteomic analysis in the future and broadens the application of MXene in the biological field.
研究领域:
分析化学
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生物化学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2021
·
IF 9.028
Shuhui Sun,Chao Shan,Zhichao Yang,Shu Wang,Bingcai Pan
Phosphonate is an important category of highly soluble organophosphorus in contaminated waters, and its oxidative transformation into phosphate is usually a prerequisite step to achieve the in-depth removal of the total phosphorus. Currently, selective oxidation of phosphonate into phosphate is urgently desired as conventional advanced oxidation processes suffer from severe matrix interferences. Herein, we employed 1-hydroxyethylidene-1,1-diphosphonic acid (HEDP) as a model phosphonate and demonstrated its efficient and selective oxidation by the Cu(II)/H2O2 process at alkaline pH. In the presence of trace Cu(II) (0.020 mM), 90.8% of HEDP (0.10 mM) was converted to phosphate by H2O2 in 30 min at pH 9.5, whereas negligible conversion was observed by UV/H2O2 or a Fenton reaction (pH = 3.0). The oxidation of HEDP by Cu(II)/H2O2 was insignificantly affected by natural organic matters (10.0 mg TOC/L) and various anions including chloride, sulfate, and nitrate (10.0 mM). The complexation of Cu(II) with HEDP coupling Cu(III) produced in situ enabled an intramolecular electron transfer process, which features high selective oxidation. Selective degradation of HEDP was further validated by adding stoichiometric H2O2 into an industrial effluent, where the existing Cu(II) could serve as the catalyst. This study also provides a successful case to trigger selective oxidation of trace pollutants of concern upon synergizing with the nature of the contaminated water.
研究领域:
环境科学
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水处理技术
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 19
·11256
·
IF 6.208
Bo Xu,Yanting Liu,Simiao Wei,Siheng Zhao,Lijun Qian,Yajun Chen,Hao Shan,Qinglei Zhang
A phosphorous-based bi-functional compound HPDAl was used as a reactive-type flame retardant (FR) in an epoxy thermoset (EP) aiming to improve the flame retardant efficiency of phosphorus-based compounds. HPDAl, consisting of two different P-groups of aluminum phosphinate (AHP) and phosphophenanthrene (DOPO) with different phosphorous chemical environments and thus exerting different FR actions, exhibited an intramolecular P-P groups synergy and possessed superior flame-retardant efficiency compared with DOPO or AHP alone or the physical combination of DOPO/AHP in EP. Adding 2 wt.% HPDAl made EP composites acquire a LOI value of 32.3%, pass a UL94 V-0 rating with a blowing-out effect, and exhibit a decrease in the heat/smoke release. The flame retardant modes of action of HPDAl were confirmed by the experiments of the scanning electron microscope (SEM), X-ray photoelectron spectroscopy (XPS), and thermogravimetry–Fourier transform infrared spectroscopy–gas chromatograph/mass spectrometer (TG-FTIR-GC/MS). The results indicate that the phosphorous-based FRs show different influences on the flame retardancy of composites, mainly depending on their chemical structures. HPDAl had a flame inhibition effect in the gas phase and a charring effect in the condensed phase, with a well-balanced distribution of P content in the gas/condensed phase. Furthermore, the addition of HPDAl hardly impaired the mechanical properties of the matrix due to the link by chemical bonds between them.Keywords:flame retardant;intramolecular P-P synergy;aluminum phosphinate;phosphophenanthrene;epoxy resin
研究领域:
材料科学
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化学工程
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2021
·Vol. 102
·期 4
·1640-1650
·
IF 3.639
Zongming Sui,Jie Yin,Jianguo Huang,Ling Yuan
BACKGROUND Some soil microorganisms can mobilize unavailable phosphorus (P) in soils for plant use and increase P fertilizer efficiency. Thus, an abiotic P solubilization experiment and fungal incubation in solution and soil were conducted to investigate the mobilization of various P compounds by a new white-rot fungus Ceriporia lacerata HG2011. The crop agronomic performances were then evaluated in the winter barley–summer maize–winter wheat rotation field. RESULTS Ceriporia lacerata HG2011 had a wide P mobilization spectrum and mobilized P by different mechanisms depending on P sources supplied in liquid culture. The chief mechanism employed by this fungus was the production of protons in mobilizing Ca 3 (PO 4 ) 2 , low-molecular-weight organic acids and other unknown substances in FePO 4 and AlPO 4 , phytase (an inducible enzyme in the presence of phytate) in phytate, and phosphatase in lecithin and ribonucleic acid, respectively. As a result of the large fungal biomass, P accumulated in the hypha should also be considered in the assessment of the fungal P mobilization, and not just only soluble inorganic P. As C. lacerata HG2011 colonized on and in the test soil, phosphatase and phytase activities were enhanced but pH decreased in the soil, leading to P mobilization. The application of this fungus mobilized soil P, increased crop P uptake and yields, and consecutively reduced P fertilizer use without yield sacrifices in the multiple crop rotation field. CONCLUSION C. lacerata HG2011 showed a new use with respect to mobilizing soil P and reducing P fertilizer input in modern agriculture beyond medical purposes, environmental protection and biofuel production. © 2021 Society of Chemical Industry.
研究领域:
农业科学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Yihao Li,Nan Zhang,Jiarui Xu,Lijuan Liu,Xiaochuang Cao,Xianyong Lin,Chengliang Sun
The deficiency of essential mineral nutrients caused by xenobiotics often results in plant mortality or an inability to complete its life cycle. Imazethapyr , a widely utilized imidazolinone herbicide, has a long-lasting presence in the soil-plant system and can induce toxicity in non-target plants. However, the effects of imazethapyr on mineral nutrient homeostasis remain poorly comprehended. In this study, Arabidopsis seedlings exposed to concentrations of 4 and 10 μg/L imazethapyr showed noticeable reductions in shoot development and displayed a distinct dark purple color, which is commonly associated with phosphorus (P) deficiency in crops. Additionally, the total P content in both the shoots and roots of Arabidopsis significantly decreased following imazethapyr treatment when compared to the control groups. Through the complementary use of physiological and molecular analyses, we discovered that imazethapyr hinders the abundance and functionality of inorganic phosphorus (Pi) transporters and acid phosphatase . Furthermore, imazethapyr impairs the plant's Pi-deficiency adaptation strategies, such as inhibiting Pi transporter activities and impeding root hair development, which ultimately exacerbate P starvation. These results provide compelling evidence that residues of imazethapyr have the potential to disrupt plant P homeostasis and acquisition strategies. These findings offer valuable insights for risk assessment and highlight the need to reconsider the indiscriminate use of imazethapyr, particularly under specific scenarios such as nutrient deficiency.
研究领域:
植物学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Food & Function
·
2021
·Vol. 12
·期 5
·2282-2291
·
IF 5.396
Mengqi Zhang,Peihai Li,Fengxia Wang,Shanshan Zhang,Haonan Li,Yun Zhang,Ximin Wang,Kechun Liu,Xiaobin Li
Phospholipids not only have high nutritional value, but also have a positive effect on cardiovascular disease, cancer and nervous system diseases. However, the activity of individual phospholipid classes of shrimp phospholipids is rarely studied. This paper researched phospholipids in the by-products of Penaeus vannamei processing. The phospholipid classes of the head from P. vannamei (PV) were separated by column chromatography, analyzed with UHPLC-Q-Exactive HF/MS, and quantified using ammonium ferrothiocyarate spectrophometry. In addition, their cardiovascular activities in zebrafish models were evaluated. A total of 5 phospholipid classes were obtained, including PV-PC, PV-PE, PV-PI, PV-PS and PV-SM, and identified as phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylserine (PS) and sphingomyelin (SM), respectively. In the phospholipid profiling analysis, PV-PC (308 molecules) had the highest proportion with 85.24%, followed by PV-PE (139 types) with 9.32%, PV-SM (41 structures) with 4.75%, PV-PS (24 types) with 0.16%, and PV-PI (6 molecules) with 0.03%. In the quantitative analysis, the content of PV was 45.7%, and the purity of phospholipid classes was 75.5–88.1%. In the cardiovascular activity assays, the effects of different phospholipid classes were different. For example, PV-PC groups had strong angiogenesis activity, but PV-PE groups showed the opposite property. Our comprehensive profiling analysis and in vivo bioactivity evaluation of phospholipids from the head of P. vannamei can provide evidence for their targeted applications in the future.
研究领域:
营养学
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心血管科学
·
脂质组学
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水产养殖
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL MONITORING AND ASSESSMENT
·
2020
·Vol. 192
·期 6
·1-9
·
IF 1.903
Li Xuguang,Elgarhy Abdelbaky Hossam,Hassan Mohamed Elfatih,Chen Yanbo,Liu Guanglong,ElKorashey Reham
Existing research on phosphorus removal from wastewater mostly focused on inorganic phosphorus while ignoring organic phosphorus, which has potential bioavailability. This study aims to provide an innovation for the development of advanced treatment material for both inorganic and organic phosphorus removal in water. In this study, ferrihydrite loaded on the graphene oxide (FeOOH-GO) composite adsorbent was synthesized by surface precipitation method, and its ability to remove both phosphate and diazinon as forms of inorganic and organic phosphorous from water was investigated. Characterization of the loaded composite using X-ray diffraction (XRD), scanning electron microscope (SEM), and Fourier transform-infrared spectroscopy (FTIR) indicated that FeOOH was successfully loaded onto graphene. The results of batch adsorption experiments showed that the adsorbent could remove both inorganic and organic phosphorus compounds simultaneously from water. When FeOOH content is 40%, the equilibrium adsorption amount of FeOOH-GO composite adsorbent for phosphate and diazinon was 5.81 and 23.20 mg g −1 , respectively. Environmental parameters such as pH and initial concentration have important influence on phosphorus removal by FeOOH-GO composite adsorbent and the removal efficiency of the inorganic and organic phosphorus from water decreases by increasing the initial concentration of phosphate and diazinon and the pH. It was concluded that the FeOOH-GO composite adsorbent has great potential to remove both inorganic and organic phosphate simultaneously from contaminated water.
研究领域:
环境科学
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2024
·
IF 2.8
FangKun Ren,JunYong Dai,JingYi Zhang,YanFei Luan,Fan Yang,Jian Shen,HaiLong Liu,JiaHong Zhou
The specific enrichment of multi-phosphopeptides in the presence of non-phosphopeptides and mono-phosphopeptides was still a challenge for phosphoproteomics research. Most of these enrichment materials relied on Zn, Ti, Sn, and other rare precious metals as the bonding center to enrich multi-phosphopeptides while ignoring the use of common metal elements. The addition of rare metals increased the cost of the experiment, which was not conducive to their large-scale application in biomedical proteomics laboratories. In addition, multiple high-speed centrifugation steps also resulted in the loss of low-abundance multi-phosphopeptides in the treatment procedure of biological samples. This study proposed the use of calcium, a common element, as the central bonding agent for synthesizing magnetic calcium phosphate materials (designated as CaP-Fe 3 O 4 ). These materials aim to capture multi-phosphopeptides and identifying phosphorylation sites. The current results demonstrate that CaP-Fe 3 O 4 exhibited excellent selection specificity, high sensitivity, and stability in the enrichment of multi-phosphopeptides and the identification of phosphorylation sites. Additionally, the introduction of magnetic separation not only reduced the time required for multi-phosphopeptides enrichment but also prevented the loss of these peptides during high-speed centrifugation. These findings contribute to the widespread application and advancement of phosphoproteomics research.
研究领域:
生物化学
·
蛋白质组学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）