Acta Biomaterialia
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2022
·
IF 10.633
Mengying Chen,Huaping Tan,Weijie Xu,Zijia Wang,Jinglei Zhang,Shengke Li,Tianle Zhou,Jianliang li,Xiaohong Niu
Injectable hydrogels based on various functional biocompatible materials have made rapid progress in the field of bone repair . In this study, a self-healing and injectable polysaccharide-based hydrogel was prepared for bone tissue engineering. The hydrogel was made of carboxymethyl chitosan (CMCS) and calcium pre-cross-linked oxidized gellan gum (OGG) cross-linked by the Schiff-base reaction. Meanwhile, magnetic hydroxyapatite/gelatin microspheres (MHGMs) were prepared by the emulsion cross-linking method. The antibacterial drugs, tetracycline hydrochloride (TH) and silver sulfadiazine (AgSD), were embedded into the MHGMs. To improve the mechanical and biological properties of the hydrogels, composite hydrogels were prepared by compounding hydroxyapatite (HAp) and drug-embedded MHGMs. The physical, chemical, mechanical and rheological properties of the composite hydrogels were characterized, as well as in vitro antibacterial tests and biocompatibility assays, respectively. Our results showed that the composite hydrogel with 6% (w/v) HAp and 10 mg/mL MHGMs exhibited good magnetic responsiveness, self-healing and injectability. Compared with the pure hydrogel, the composite hydrogel showed a 38.8% reduction in gelation time (196 to 120 s), a 65.6% decrease in swelling rate (39.4 to 13.6), a 51.9% increase in mass residual after degradation (79.5 to 120.8%), and a 143.7% increase in maximum compressive stress (53.6 to 130.6 KPa). In addition, this composite hydrogel showed good drug retardation properties and antibacterial effects against both S. aureus and E. coli . CCK-8 assay showed that composite hydrogel maintained high cell viability (> 87%) and rapid cell proliferation after 3 days, indicating that this smart hydrogel is expected to be an alternative scaffold for drug delivery and bone regeneration . Statement of significance Biopolymer hydrogels have been considered as the promising materials for the treatment of tissue engineering and drug delivery. Injectable hydrogels with and self-healing properties and responsiveness to external stimuli have been extensively investigated as cell scaffolds and bone defects, due to their diversity and prolonged lifetime. Magnetism has also been involved in biomedical applications and played significant roles in targeted drug delivery and anti-cancer therapy. We speculate that development of dual cross-linked hydrogels basing biopolymers with multi-functionalities, such as injectable, self-healing, magnetic and anti-bacterial properties, would greatly broaden the application for bone tissue regeneration and drug delivery.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2022
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IF 5.518
Sirui He,Jia Liu,Shu He,An Liu,Wei Shao
In this work, a double crosslinking strategy comprising chemical crosslinking and physical crosslinking was applied to fabricate novel polyvinyl alcohol/gelatin/silver sulfadiazine (PGA) sponges. The morphology and chemical composition were characterized via Environmental Scanning Electron Microscopy and Fourier-transform infrared spectroscopy. The antibacterial activity of PGA sponges towards Escherichia coli, Candida albicans , and Staphylococcus aureus was evaluated via several methods: inhibition zone determination, plate counting, fluorescence staining of dead/live bacteria, bacterial morphology observation, and protein leakage determination. The PGA sponges exhibited excellent antibacterial performance owing to the silver sulfadiazine release from the sponges. Moreover, the sponges exhibited sustained sulfadiazine release, and the release profiles reasonably fitted the Higuchi model, indicating that the release was diffusion controlled. The improved mechanical properties and excellent antibacterial effect of the prepared PGA sponges demonstrate their potential as outstanding antibacterial materials.
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MACROMOLECULAR CHEMISTRY AND PHYSICS
·
2019
·Vol. 220
·期 23
·1900399
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IF 2.622
Shoukang Du,Xiaojin Chen,Xiaofei Chen,Shengke Li,Guoliang Yuan,Tianle Zhou,Jianliang Li,Yang Jia,Dangsheng Xiong,Huaping Tan
Wound dressings with pH-sensitive properties, stable mechanical performance, and antibacterial properties have great potential for clinical applications. Herein, drug-loaded microgels via Schiff-base reaction between carboxymethyl chitosan and oxidized carboxymethyl cellulose are fabricated. Then they are embedded in hydrogels to form a hydrogel-microgels composite (Gel/MGs). The gelation time, morphologies, swelling ratio, weight loss ratio, mechanical properties, pH-sensitive drug release profiles, and antibacterial activities are examined. Adding microgels endows the hydrogels with more stable properties, enhanced mechanical performance, and drug release sensitivity to both acid and alkali in vitro. Gel/MGs dressings show a steady mass after 6 h and even maintain its 95.4% initial mass after 14 days in pH 7.4. In pH 9.5, Gel/MGs dressings also show a higher cumulative release of silver sulfadiazine than that in pH 7.4 and pH 5.5. Gel/MGs/AgSD dressings demonstrate desirable antibacterial properties, which may ensure their potential application in wound dressing.
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MATERIALS CHEMISTRY AND PHYSICS
·
2019
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IF 2.781
Sugunya Potiwiput,Huaping Tan,Guoliang Yuan,Shengke Li,Tianle Zhou,Jianliang Li,Yang Jia,Dangsheng Xiong,Xiaohong Hu,Zhonghua Ling,Yong Chen
Dual-crosslinked alginate/carboxymethyl chitosan (CMC) based hydrogels containing in situ synthesized calcium phosphate (CaP) particles were developed by combining two physical crosslinking, the ionic crosslinking of alginate with divalent cation (Ca 2+ ), and the electrostatic interaction between cation of amino groups on CMC and anion of carboxyl groups on alginate. The calcium phosphate particles were in situ synthesized by using the excess Ca 2+ of ionic crosslinking. The developed hydrogels were investigated the rheological properties, swelling behavior, degradation, and drug released performance in both water-soluble and insoluble drug models. The results showed that the viscoelasticity and swelling capacity of dual-crosslinked hydrogels were enhanced comparing the single-crosslinked alginate-based hydrogel. The in situ gelation of the developed hydrogel provided the injectable ability and drug-encapsulated capacity. Beside, calcium phosphate, including hydroxyapatite (HAp) was in situ synthesized during the gelation. Biocompatibility of this injectable hydrogel was studied by encapsulation behavior of human adipose-derived stem cells (ASCs). With these qualities, the developed hydrogel may have potential for medical providers such as hydrogel injections and drug providers.
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Journal of the Taiwan Institute of Chemical Engineers
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2016
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IF 2.848
Wei Shao,Hui Liu,Jimin Wu,Shuxia Wang,Xiufeng Liu,Min Huang,Peng Xu
Bacterial cellulose (BC) has been extensively explored as some of the most promising biomaterials for biomedical applications. However, BC does not have intrinsically antibacterial property. In the present study, antibacterial BC-silver sulfadiazine (AgSD) composites were prepared by a simple blend method since AgSD is a topical antibacterial agents used as a topical cream on burns. The prepared composites were characterized by several techniques including SEM, FTIR, XRD and TG. These results indicate AgSD was successfully impregnated into BC matrix. The releases of Ag + and SD − ions from BC-AgSD composites at different pH values were studied, which showed pH-sensitive controlled release behaviors. The antibacterial performances of BC-AgSD composites were evaluated with Staphylococcus aureus and Candida albicans . Moreover, the cytotoxicity of BC-AgSD composites was performed on HEK293 cells. The present work demonstrates a facile way to prepare BC-AgSD composites with excellent antibacterial activities and good biocompatibility, which can be used as potential wound dressings.
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CARBOHYDRATE POLYMERS
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2016
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IF 4.219
Wei Shao,Jimin Wu,Shuxia Wang,Min Huang,Xiufeng Liu,Rui Zhang
Chitosan/silver sulfadiazine (CS/AgSD) composite sponges were prepared by incorporating AgSD particles into CS matrix in order to develop novel biomaterials for wound healing. The co-existence of CS and AgSD in the composite sponges was confirmed by SEM, FTIR and XRD spectra, respectively. The porosity and swelling properties of the fabricated CS/AgSD composite sponges were examined and the results indicated that they had high porosity and excellent swelling behaviors. Antibacterial property of the CS/AgSD composite sponges was also performed against E. coli , C. albicans , S. aureus and B. subtilis , respectively. The results showed that they possessed broad spectrum of antibacterial activity. The cytotoxicity of the prepared composite sponges was performed by MTT viability assay and fluorescence staining method on HEK293 cell lines. The cell viability results indicated that the designed sponges had no significant cytotoxicity. The results of this study demonstrated that the CS/AgSD composite sponges have potential application in antimicrobial wound dressing materials.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Xinwei Tao,Zijia Wang,Bowen Ren,Jianliang Li,Tianle Zhou,Huaping Tan,Xiaohong Niu
Flexible chitosan-based membranes were prepared by casting/solvent evaporation method using chitosan flocks as raw material. To improve mechanical and biological properties, chitosan microspheres (CMs) were prepared and integrated to form the composite membranes. Two different anti-bacterial drugs, e.g., tetracycline hydrochloride (TH) and silver sulfadiazine (AgSD), were loaded into the CMs and composite membranes to enhance their anti-bacterial properties. Furthermore, composite membranes were alternately coated by multi-layers of oxidized alginate (OAlg) and carboxymethyl chitosan (CMCS) via the layer-by-layer self-assembly and Schiff-base cross-linking. Our results demonstrated that the microspheres and multi-layer coatings could improve the swelling, water vapor transmission and hydrophilicity of the composite membranes. The chitosan microspheres and multi-layer coatings increased the tensile strength and decreased the elongation at the break of the membranes. Our composite membrane had better mechanical properties, slow drug release, anti-bacterial properties, which could promote cell proliferation. This composite membrane has great application potential in inhibiting bacterial infection and promoting wound regeneration.
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Polymers
·
2018
·Vol. 10
·期 2
·190
·
IF 2.935
Sa Liu,Chunting Zhong,Weiwei Wang,Yongguang Jia,Lin Wang,Li Ren
As a drug carrier, polyrotaxane (PR) has been used for targeted delivery and sustained release of drugs, whereas silver sulfadiazine (SD-Ag) is an emerging antibiotic agent. PR was synthesized by the use of α-cyclodextrin (CD) and poly(ethylene glycol) (PEG), and a specific antibacterial material (PR-(SD-Ag)) was then prepared by loading SD-Ag onto PR with different mass ratios. The loading capacity and the encapsulation efficiency were 90% at a mass ratio of 1:1 of PR and SD-Ag. SD-Ag was released stably and slowly within 6 d in vitro, and its cumulative release reached more than 85%. The mechanism of PR loading SD-Ag might be that SD-Ag attached to the edge of α-CD through hydrogen bonding. PR-(SD-Ag) showed a higher light stability than SD-Ag and held excellent antibacterial properties againstEscherichia coli(E. coli) andStaphylococcus aureus(S. aureus).Keywords:polyrotaxane;silver sulfadiazine;mechanism;antibacterial
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Materials Today Bio
·
2025
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IF 10.2
Xinming Wang,Haonan Zhang,Weichang Xie,Bei Qian,Shixing Huang,Qiang Zhao,Xiaofeng Ye
Complete tissue recovery following traumatic injury remains a major clinical challenge. While tissue adhesives show promise for managing traumatic injuries, developing materials with robust wet adhesion and high biocompatibility remains difficult. Decellularized extracellular matrix (ECM)-derived materials are widely utilized in tissue engineering due to their superior biocompatibility and bioactivity. In this study, a wet adhesive is developed by functionalizing ECM with dopamine. The resulting ECM-dopamine exhibits strong wet adhesion and excellent biocompatibility. Furthermore, ECM-dopamine can be engineered into a drug delivery platform for small agents and macromolecules. Solid lipid nanoparticles (SLNs) are incorporated into ECM-dopamine to enable sustained release of small molecules. The ECM-dopamine-SLN system ensures sustained drug release for at least one week upon adhesion to target tissues. ECM-dopamine-SLN loaded with antimicrobials accelerates wound healing and promotes angiogenesis by modulating the inflammatory response in a mouse skin excision model. Additionally, ECM-dopamine can deliver bioactive macromolecules to injured tissue. ECM-dopamine loaded with insulin-like growth factor-1 promotes skeletal muscle regeneration in a mouse volumetric muscle loss model, likely through the modulation of M2-like macrophage polarization. The dual functionality of ECM-dopamine as both a wet adhesive and a drug delivery platform offers significant potential for regenerative medicine applications.
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