CHEMOSPHERE
·
2024
·
IF 8.1
Guowen Tang,Yuling Chen,Shanshan Lin,Xiaolin Li
Sulfadoxine (SDX) is a broad-spectrum veterinary antibiotic, which was used alone for the treatment of various infections in the past, and detected ubiquitously in the aqueous environment. However, understanding SDX's photo- and microbial degradation within the environment, especially in marine matrixes, remains limited. This research hones in on SDX's degradation dynamics in seawater. Photodegradation emerges as the dominant process, surpassing microbial degradation in speed and efficiency. Notably, 90% of SDX is photo-degraded within 12 h, while only 52% is removed via microbial degradation over two weeks. Time-of-flight mass spectrometry provides high-resolution molecular mass information on degradation products . The molecular structures of hydrolysis, photo-, and microbial degradation products are deduced from accurate precursor and fragment ion masses, alongside an integrated data processing workflow. Six hydrolysis products arise from the treatment, and photodegradation and microbial degradation yield nine and eighteen products, respectively. Molecular insights from these products inform plausible degradation pathways involving hydrolysis, photodegradation, and microbial degradation. Processes like bond cleavage, methylation , hydroxylation, oxidation, reduction, and methoxylation are identified and associated with degradation. This study presents a comprehensive workflow for acquiring and processing degradation product data linked to emerging organic pollutants. Moreover, it contributes to our comprehension of the environmental fate of veterinary drugs in marine ecosystems .
研究领域:
环境科学
·
药理学
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DOI
PubMed
高影响因子
WATER RESEARCH
·
2025
·
IF 12.4
Junwei Liu,Xinyue Yang,Tao Gao,Yuehan Zhang,Dandan Pan,Jian He,Xiangwei Wu
The widespread use of sulfamethoxazole (SMX) has raised significant concerns regarding environmental residue pollution and the emergence of bacterial antibiotic resistance. Anaerobes play crucial roles in the biogeochemical element cycle and the degradation of organic pollutants; however, the pure-cultured anaerobes involved in SMX anaerobic degradation and their underlying mechanisms remain largely unknown. In this study, a novel anaerobic bacterium, Cupidesulfovibrio sp. strain SA-9, was isolated and characterized for its ability to degrade SMX. Strain SA-9 preferentially utilized lactate or pyruvate as electron donors and sulfate, nitrate, or carbonate as electron acceptors to drive SMX degradation. The degradation T 1/2 values of SMX at concentrations ranging from 10 to 800 μM were between 6.0 and 17.2 d under optimal conditions which included pH 7.0-8.0, 30–37°C, sulfate as the electron acceptor, and pyruvate as the electron donor. Strain SA-9 degraded SMX via isoxazole ring cleavage, acetylation, and hydroxylation, and the ECOSAR-predicted results and bacterial growth inhibition assays indicated that these pathways was detoxifying. Multi-omics analysis and heterologous expression revealed that strain SA-9 degraded SMX via a reductive transformation pathway fueled by electron donors oxidized through the TCA cycle, with electrons transferred through a chain involving flavin-dependent thymidylate synthase (FDTS) and ferredoxin (Fd) to reduce electron acceptors that provide reducing power for SMX degradation. Furthermore, the degradation half-life ( T 1/2 ) values of SMX at 10 and 50 μM were 1.7 and 1.8 d in actual wastewater. Strain SA-9 significantly enhanced SMX removal and mitigated the dissemination risk of ARGs sul1 and sul2 in actual wastewater. This study elucidates the anaerobic microbial resistance mechanism to SMX and highlights the potential of strain SA-9 for the bioremediation of SMX-contaminated water.
研究领域:
环境微生物学
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污染物生物降解
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厌氧代谢
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抗生素抗性基因
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分子生态学
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药物代谢
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水环境修复
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微生物生理学
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2026
·
IF 11.8
Tai Ye,Mei Xue,Bingjing Zhou,Shuwei Kang,Zhaobao Chen,Zilong Tang,Shihui Yang,Qianling Zhao,Min Yuan,Jinsong Yu,Hui Cao,Liling Hao,Xiuxiu Wu,Fengqin Yin,Fei Xu
One-pot autocatalytic CRISPR biosensing offers a promising route for signal amplification without nucleic acid pre-amplification, but its efficiency is limited by an intrinsic readout-amplification conflict: the ssDNA reporter required for signal output competes with the autocatalytic mediator required for feedback amplification. Here, we report a biphasic spatiotemporal regulation strategy (BS-Cas12a system) to resolve this substrate competition for one-pot autocatalytic CRISPR biosensing of non-nucleic-acid targets. Mechanistic studies revealed that Cas12a preferentially cleaved the ssDNA reporter over the autocatalytic circular mediator, leading to insufficient mediator linearization and impaired autocatalytic amplification. A glycerol/water biphasic system was therefore constructed to spatially delay reporter access to activated Cas12a, allowing preferential cleavage of the autocatalytic mediator and subsequent generation of additional Cas12a activators. This biphasic system achieved a 3-fold increase in the autocatalytic amplification efficiency. By integrating an aptamer-mediated target-to-activator conversion module, non-nucleic-acid recognition was programmably converted into Cas12a activation. Using sulfadimethoxine as a model target, the platform achieved a detection limit of 65 pM and showed good recoveries in fish samples. The system was further extended to Cd 2+ and thrombin detection with limits of detection of 0.65 nM and 11.63 pM, respectively. Moreover, by replacing the fluorescent reporter with a FAM/biotin-labeled reporter, a lateral-flow readout was achieved. This work provides a kinetic-regulation strategy for modular one-pot Cir DNA autocatalytic biosensing with an independent and exchangeable reporter and expands CRISPR-based detection toward diverse non-nucleic-acid targets.
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产品关联
DOI
高影响因子
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
·
2025
·
IF 4.7
Aolin Ma,Siqi Liu,Zhixuan Du,Longfei Zhu,Guanping Feng
Sulfonamides, owing to their therapeutic properties and cost-effectiveness, are widely employed in clinical settings and the animal husbandry industry. However, the misuse of these compounds has resulted in severe water pollution, thereby posing significant threats to the growth and development of aquatic plants such as duckweed. Our controlled experiments revealed that sulfonamide exposure disrupts the plasma membrane integrity in duckweed root tip cortex cells and inhibits the root growth. Utilizing ESI-HPLC-MS/MS, we identified that sulfonamide exposure elicits an immune response in duckweed, characterized by the generation of substantial amounts of salicylic acid (SA). The exogenous application of SA exacerbated the sulfonamide-induced growth inhibition, whereas the addition of the SA synthesis inhibitor 1-aminobenzotriazole (ABT) mitigated the stress induced by sulfonamides. A loss-of-function mutant of ICS1, a key enzyme in the SA biosynthesis pathway, exhibited complete insensitivity to sulfonamide exposure. Furthermore, transcription factors SARD1 and CBP60g, which regulate ICS1 gene expression, were induced by sulfonamides, and their double mutant showed insensitivity to sulfonamide exposure. Reactive oxygen species (ROS) detection indicated abnormal accumulation of ROS in duckweed roots under sulfonamide exposure, which is likely to induce SA synthesis. These findings suggest that sulfonamide-mediated growth inhibition in plants is dependent on the ICS1-catalyzed SA biosynthesis pathway, thereby enhancing our understanding of the effects and mechanisms of sulfonamide exposure on aquatic plants.
研究领域:
植物学
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植物生理学
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环境毒理学
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生物化学
·
分子生物学
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产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2024
·
IF 3.8
Yong Zhang,Jing Wang,Fubin Liu,Litao Wang,Guihua Gao
This study describes the preparation of a cylindrical polymer foam column termed Chitosan/β-Cyclodextrin/MIL-68(Al) (CS/β-CD/MIL-68(Al)). An ice template-freeze drying technique was employed to prepare the CS/β-CD/MIL-68(Al) foam column by embedding MIL-68(Al) in a polymer matrix comprising cross-linked chitosan (CS) and β-cyclodextrin (β-CD). The cylindrical CS/β-CD/MIL-68(Al) foam was subsequently inserted into a syringe to develop a solid phase extraction (SPE) device. Without the requirement for an external force, the sample solution passed easily through the SPE column thanks to the porous structure of the CS/β-CD/MIL-68(Al) foam column. Moreover, the CS/β-CD/MIL-68(Al) foam column was thought to be a superior absorbent for SPE since it included the adsorptive benefits of CS, β-CD, and MIL-68(Al). The SPE was utilized in conjunction with high-performance liquid chromatography to analyze six sulfonamides found in milk, urine, and water. With matrix effects ranging from 80.49 % to 104.9 % with RSD values of 0.4–14.0 %, the method showed high recoveries ranging from 80.6 to 107.4 % for water samples, 93.4–105.2 % for urine, and 87.4–100.9 % for milk. It also demonstrated good linearity in the range of 10–258 ng·mL -1 with the limits of detection ranging from 1.88 to 2.58 ng·mL -1 . The cylindrical CS/β-CD/MIL-68(Al) foam column prepared in this work offered several advantages, including its simple fabrication, excellent water stability, absence of pollutants, biodegradability, and reusability. It is particularly well-suited for SPE. Furthermore, the developed SPE method, employing CS/β-CD/MIL-68(Al) foam column, is straightforward and precise, and its benefits, including affordability, ease of preparation, lack of specialized equipment, and solvent economy, underline its broad applicability for the pretreatment of aqueous samples.
研究领域:
分析化学
·
环境科学
·
食品科学
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2026
·
IF 4.8
Huiting Feng,Yuxin Yang,Ruiling Li,Yuting Yang,Zongwen Wang,FengFu Fu,Yue Lin
A variety of sulfonamides (SAs) are extensively utilized in animal husbandry and aquaculture, necessitating the development of high-throughput methods for the rapid screening and accurate determination of multiple, ideally all commonly-applied SAs, in animal-derived foodstuffs. We herein developed a paper-based colorimetric aptasensor for the detection of eight different SAs employing a mixture of two broad-specific aptamers as bio-receptor, ascorbic acid (AA)-mediated gold nanobipyramids (AuNBPs) growth for generating color signals, and a paper-based analytical device (PAD) for stabilizing and enhancing color signals. The aptasensor exhibits target SAs concentration-corresponding multiple color changes and has excellent specificity. It can be used to visually screen eight SAs (either individually or collectively) with a visual detection limit (LOD) of 4.0 μM, and quantitatively detect these SAs (either individually or collectively) with a spectrometry LOD of 0.2 μM. Importantly, the signals of the aptasensor can be stably stored for one month for later verification, and the spectrometry LOD is lower than the maximum residue limit (MRL) of total SAs in animal-derived foodstuffs. The aptasensor was successfully applied to detect eight SAs (either individually or collectively) in milk samples with a recovery of 89% - 95% and a relative standard deviation (RSD, n = 5) < 8%. The results obtained with the aptasensor were consistent with those obtained using commercial ELISA kit. These findings demonstrated that the aptasensor simultaneously meets the requirement of instrument-free screening and quantitative determination of eight SAs in animal-derived foods, making it a promising approach for ensuring the consumption safety of animal-derived foodstuffs.
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产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2021
·
IF 4.759
Houmei Liu,Pian Jin,Mi Jiang,Yu Duan,Guozheng Zhu,Hui Yu,Hongdeng Qiu
In this work, for the first time five amine-ligands including mono-amine, di-amine, tri-amine, secondary and tertiary amine, were functionalized on mesoporous micro-silicas and developed as stationary phases for hydrophilic interaction liquid chromatography (HILIC). The investigations about the retention mechanisms, effects of different chromatographic conditions and stability were systematically conducted. Three kinds of polar and hydrophilic compounds (saccharides, sulfonamides, nucleosides and nucleobases) were selected as probe molecules to evaluate their separation performances. Among the five stationary phases, only aminopropyl-bonded silica has already gained wide developments and applications. Whereas, there are no related researches about the other four to be utilized as separation media. By a series of chromatographic evaluations, the results revealed the other four mesoporous micro-silica materials functionalized with di-amine, tri-amine, secondary and tertiary amine, had great potential to be explored as novel stationary phases of HILIC. Particularly, the two stationary phases functionalized with di-amine and tri-amine exhibited outstanding separation and retention abilities. This work offered some insights on the understanding of retention in HILIC mode and provided us possibility to explore other amine-based HILIC stationary phases.
研究领域:
分析化学
·
色谱科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2020
·
IF 7.489
Yinming Fan,Guolong Zeng,Xiaoguo Ma
This work systematically investigated the effects of prepolymerization on the property of multi-templates surface molecularly imprinted polymer (MIP), which was utilized to quickly and simultaneously separate and detect six sulfonamides (sulfadiazine, sulfathiazole , sulfamerazine , sulfamethazine , sulfamethoxazole and sulfadoxine) in real waters. The MIPs were prepared using the six sulfonamides as the templates, mesoporous silica supported onto the surface of magnetic graphene oxide as the carrier and 4-vinylbenzoic as the functional monomer , with and without prepolymerization of templates with functional monomer. The preparation and adsorption conditions were optimized. It was found that the adsorption capacities of the selected six sulfonamides on the MIP (pre) prepared with prepolymerization were apparently higher than those on the MIP (no pre) synthesized without prepolymerization. Subsequently, the MIPs were utilized as adsorbents for SPE of these sulfonamides, and coupled with high performance liquid chromatography (HPLC) for the determination of sulfonamides. The developed analytical method showed outstanding applicability for the detection of trace sulfonamides in real water samples. The spiked recoveries and relative standard deviations (RSDs) of the six sulfonamides using MIP (no pre) and MIP (pre) were 73.34–99.43% and 87.37–102.34%, 2.28–7.77% and 3.18–6.49%, respectively.
研究领域:
环境科学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2020
·
IF 4.192
Mi Li,Lingling Rong,Shifan Zhou,Xiaoyu Xiao,Ligui Wu,Yuxing Fan,Conghui Lu,Xiaoming Zou
Sulfonamides (SAs) are widespread in soils, and their dissipation behavior is important for their fate, risk assessment, and pollution control. In this work, we investigated the dissipation behavior of different SAs in a soil under aerobic condition, focusing on revealing the relationship between overall dissipation (without sterilization and in dark) and individual abiotic (sorption, hydrolysis)/biotic (with sterilization and in dark) factors and taxonomy/function of microbiomes. The results showed that dissipation of all SAs in the soil followed the pseudo-first-order kinetic model with dissipation time at 50% removal (DT50) of 2.16–15.27 days. Based on, experimentally, abiotic/biotic processes and, theoretically, partial least-squares modeling, a relationship between overall dissipation and individual abiotic/biotic factors was developed with microbial degradation as the dominant contributor. Metagenomic analysis showed that taxonomic genera like Bradyrhizobium/Sphingomonas/Methyloferula and functions like CAZy family GT51/GH23/GT2, eggNOG category S, KEGG pathway ko02024/ko02010, and KEGG ortholog K01999/K03088 are putatively involved in SA microbial degradation in soil. Spearman correlation suggests abundant genera being multifunctional. This study provides some new insights into SA dissipation and can be applied to other antibiotics/soils in the future.
研究领域:
环境科学
·
微生物学
·
土壤学
·
宏基因组学
·
药理学
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产品关联
DOI
PubMed
高影响因子
Food Analytical Methods
·
2018
·Vol. 11
·期 10
·2885-2896
·
IF 2.245
Qi Mengyu,Tu Chunyan,Li Zhaoqian,Wang Weiping,Chen Jianrong,Wang Ai-Jun
A rapid, sensitive, and economical pipette tip solid phase extraction (PT-SPE) based on graphene oxide/polypyrrole (GO/PPy) coupled with high performance liquid chromatography (HPLC) was developed for the extraction and determination of sulfonamides (SAs) in honey and milk samples. The PT-SPE procedure involved only 3.0 mg of adsorbent, 3 min adsorptive extraction time, and less than 1.0 mL of solvent consumption (sample, washing, or eluent). Under the optimal conditions, good linearities (0.01–10.00 μg mL −1 ) were obtained with correlation coefficients ( r ) > 0.9996. The limits of detection ( S / N = 3) were in the range of 1.04–1.50 ng mL −1 . Meanwhile, the proposed method was successfully applied for the analysis of SAs in honey and milk with average recoveries within 62.3–109.0% and relative standard deviations less than 11.2%. The results demonstrated that the developed PT-SPE-HPLC method was suitable for the analysis of SAs in complex matrix due to its high sensitivity and accuracy.
研究领域:
分析化学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2016
·
IF 4.035
Xiuna Jia,Pan Zhao,Xiu Ye,Lianjun Zhang,Ting Wang,Qinyu Chen,Xiaohong Hou
As a novel material, metal-organic framework/graphite oxide (MIL-101(Cr)@GO) has great potential for the pretreatment of trace analytes. In the present study, MIL-101(Cr)@GO was synthesized using a solvothermal synthesis method at the nanoscale and was applied as sorbent in the dispersive micro-solid phase extraction (DMSPE) for the enrichment of the trace sulfonamides (SAs) from milk samples for the first time. Several experimental parameters including kinds of sorbents, the effect of pH, the amount of MIL-101(Cr)@GO, ionic strength , adsorption time, desorption solvent and desorption time were investigated. Under the optimal conditions, the linear ranges were from 0.1 to 10 μg/L, 0.2–20 μg/L or 0.5–50 μg/L for the analytes with regression coefficients ( r ) from 0.9942 to 0.9999. The limits of detection were between 0.012 and 0.145 μg/L. The recoveries ranged from 79.83% to 103.8% with relative standard deviations (RSDs)<10% ( n =3). MIL-101(Cr)@GO exhibited remarkable advantages compared to MIL-101(Cr), MIL-100(Fe), activated carbon and other sorbent materials used in pretreatment methods. A simple, rapid, sensitive, inexpensive and less solvent consuming method of DMSPE-ultra-high performance liquid chromatography-tandem mass spectrometry (DMSPE-UHPLC-MS/MS) was successfully applied to the pre-concentration and determination of twelve SAs in milk samples.
研究领域:
分析化学
·
食品安全
·
药物检测
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2016
·
IF 4.035
Houmei Liu,Jia Chen,Zhan Li,Makoto Takafuji,Hirotaka Ihara,Hongdeng Qiu
In this work, a “solvent-free” and green method for controllable and quantitative preparation of chromatographic stationary phase has been proposed. About the method, the largely excess of functional monomer and strict control of the proportion between silane and silica are the two most important points. Due to the existence of excessive functional monomer, silane can be utilized at the maximum extent, thereby achieving quantitative and reproducible bonding. Meanwhile, the excessive monomer can also be well recycled. By this new method, we controllably synthesized three Sil-MIm columns with various bonding amounts to investigate how the bonding amounts influence separation performances. The experiment results stated in some cases the columns with appropriate bonding amounts rather than maximum, were the best to fulfil some specific tasks. Meanwhile, it also demonstrated how important it was to control the bonding amounts of stationary phase.
研究领域:
化学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）