TALANTA
·
2023
·
IF 6.1
Qian Chen,Yuquan Pan,Chaochen Sun,Zongwen Wang,Yongning Wu,FengFu Fu
In reality, various sulfonamides (SAs) were alternately used in animal husbandry to avoid generating drug resistance. Thus, it is crucial to develop simple and high-throughput methods for detecting multiple or groups of SAs to realize rapid screening of total SAs residues in foods. We herein developed a sensitive and efficient MnO 2 nanosheets-mediated etching of gold nanobipyramids (AuNBPs), which can generate more vivid color changes, and further fabricated a high-throughput multicolor immunosensor for the visual screening/semi-quantitative detection of 6 different SAs including sulfamethazine (SMZ), sulfamethoxydiazine (SMD), sulfisomidine (SIM), sulfamerazine (SMR), sulfamonomethoxine (SMM) and sulfaquinoxaline (SQ) by using AuNBPs as signal and broad-specificity anti-SAs antibody as a bio-receptor. The immunosensor displays more vivid color changes, and has a lower visual detection limit and excellent specificity. It can be applied to detect as little as 1.0 ng/mL of SMZ, SMD, SMR and 2.0 ng/mL of SIM, SMM, SQ by bare eye observation, and 0.2 ng/mL of above 6 SAs by UV–visible spectrophotometry. The visual detection limit of the immunosensor is much lower than the maximum residue limit of total SAs (100 μg/kg) in edible tissues. The immunosensor was successfully applied to detect SMZ, SMD, SIM, SMR, SMM and SQ in milk with a recovery of 84%–106% and a RSD (n = 5) < 8%. The success of this study provided a promising assay for the on-site rapid screening of SMZ, SMD, SIM, SMR, SMM and SQ in food by bare eye observation. Importantly, the immunosensor may be expended as a general method for the visual screening/semi-quantitative detection of the group of other antibiotics by using the corresponding broad-specificity antibody as a bio-receptor.
研究领域:
分析化学
·
纳米技术
·
食品科学
·
免疫学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Tao Ai,Mingling Yu,Yicheng Dai,Ruipu Yao,Ling N. Jin,Yuanyuan Yu,Xinbin Qiu,Peixin Huang,Xifen Zhu,Jianteng Sun,Lizhong Zhu
This study investigates the uptake, biotransformation, and phytotoxicity of sulfadiazine (SDZ) and its acetyl derivative N4-acetylsulfadiazine (NASDZ) in rice. Results showed that rice was more tolerant to NASDZ, with lower malondialdehyde and reactive oxygen species levels but higher antioxidant enzyme activities (SOD, POD, and CAT). The maximum accumulations of SDZ in roots and shoots were 19.3 ± 1.0 and 3.6 ± 1.1 μg/g, while NASDZ were 18.6 ± 2.5 and 3.5 ± 1.4 μg/g, respectively. SDZ exposure generated more metabolic intermediates, including deamination, hydroxylation, glycosylation, acetylation, and formylation products, while NASDZ metabolism was documented for the first time. Key genes involved in biotransformation include cytochrome P450, acetyltransferase, glycosyltransferases, and methyltransferase. Density functional theory calculations showed structural differences affecting reactive sites and intermediates. SDZ disrupted lipid metabolism, while NASDZ altered carbohydrate and amino acid pathways, highlighting their selective effects on rice metabolism. Our data help understand sulfonamide biotransformation and phytotoxicity in rice.
研究领域:
环境科学
·
植物生理学
·
毒理学
✓ 已验证
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2023
·
IF 8.8
Meihong Tan,Wanping Shi,Haifeng Wang,Guanglan Di,Zhengxin Xie,Shisuo Fan,Jun Tang,Fengshou Dong
The removal of antibiotics in wastewater has attracted increasing attention. Herein, a superior photosensitized photocatalytic system was developed with acetophenone (ACP) as the guest photosensitizer, bismuth vanadate (BiVO 4 ) as the host catalyst and poly dimethyl diallyl ammonium chloride (PDDA) as the bridging complex, and used for the removal of sulfamerazine (SMR), sulfadiazine (SDZ) and sulfamethazine (SMZ) in water under simulated visible light (λ > 420 nm). The obtained ACP-PDDA-BiVO 4 nanoplates attained a removal efficiency of 88.9%–98.2% for SMR, SDZ and SMZ after 60 min reaction and achieved kinetic rate constant approximately 10, 4.7 and 13 times of BiVO 4 , PDDA-BiVO 4 and ACP-BiVO 4 , respectively, for SMZ degradation. In the guest-host photocatalytic system, ACP photosensitizer was found to have a great superiority in enhancing the light absorption , promoting the surface charge separation-transfer and efficient generation of holes (h + ) and superoxide radical ( · O 2 − ) , greatly contributing to the photoactivity. The SMZ degradation pathways were proposed based on the identified degradation intermediates, involving three main pathways of rearrangement, desulfonation and oxidation. The toxicity of intermediates was evaluated and the results demonstrated that the overall toxicity was reduced compared with parent SMZ. This catalyst maintained 92% photocatalytic oxidation performance after five cyclic experiments and displayed a co-photodegradation ability to others antibiotics (e.g., roxithromycin, ciprofloxacin et al.) in effluent water . Therefore, this work provides a facile photosensitized strategy for developing guest-host photocatalysts, which enabling the simultaneous antibiotics removal and effectively reduce the ecological risks in wastewater.
研究领域:
环境科学
·
水处理
·
光催化
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2025
·
IF 6.1
Yurong Zhang,Tiejun Li,Yuxin Lin,Dongdong Xu,Haifeng Jiao
Sulfonamide antibiotics, widely used in human and veterinary medicine as well as agriculture, pose environmental concerns due to their stability and poor biodegradability. This study fills a critical gap in understanding the ecological impact of sulfonamide antibiotics on marine microalgae, particularly Skeletonema costatum , a key primary producer in marine ecosystems. This study investigated the biological responses of the marine microalga Skeletonema costatum to sulfadiazine (SD) and sulfamethoxazole (SMX). Both antibiotics significantly impacted S. costatum , with SD having a more pronounced effect. Growth studies showed a clear dose-response relationship: Low concentrations (0.5 mg/L) of SD and SMX stimulated growth, while higher concentrations (3 mg/L, 5 mg/L, and 10 mg/L) inhibited growth. The 96-hour half-maximal inhibitory concentrations (96h-IC 50 ) were 1.654 mg/L and 1.838 mg/L, respectively, initially indicating that SD has a stronger inhibitory effect on S. costatum than SMX. Photosynthetic activity, measured by chlorophyll a content and the maximum quantum yield of photosystem II ( Fv/Fm ) values, showed that low concentrations (0.5 mg/L) of SD and SMX increased photosynthetic efficiency, while high concentrations (3 mg/L, 5 mg/L, and 10 mg/L) significantly inhibited it. Antioxidants activity analysis revealed that SD and SMX exposure altered superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), glutathione reductase (GR), and malondialdehyde (MDA) levels. SOD, GR, and GSH-Px levels initially increased but later decreased, suggesting a synergistic effect, while MDA levels consistently increased, indicating oxidative stress and biochemical disruption in algal cells. The Integrated Biomarker Response Version 2 (IBRv2) index provided a comprehensive evaluation of the ecological risks posed by SD and SMX, demonstrating that these antibiotics can significantly disrupt the physiology of marine microalgae. The IBR v2 index provided a comprehensive evaluation of the ecological risks posed by SD and SMX, demonstrating that these antibiotics can significantly disrupt the physiology of marine microalgae. Higher IBR v2 values for SD exposure indicated more substantial impacts on S. costatum . This study underscores the significant ecological risks of sulfonamide antibiotics in marine environments, highlighting the need for further research and regulation to mitigate their impact.
研究领域:
环境科学
·
海洋生物学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Environmental Science-Water Research & Technology
·
2022
·Vol. 8
·期 10
·2207-2215
·
IF 5.819
Jie Ji,Changjie Shi,Luo Xu,Kai Zhang,YunShu Zhang,Cong Li,Eric Lichtfouse
Sulfonamides (SAs) have been proved to damage organisms if the contact time last over the long term. Therefore, sulfapyridine (SPD), as typical one, was chosen as a target to be investigated. For the study, the degradation kinetic of SPD in the chlorination stage of a pilot-scale water distribution system (WDS) was studied. The result showed that the degradation of SPD simultaneously followed the first-order and pseudo second-order kinetic model. In the beakers tests or WDS, the reaction rate reaches maximum value when the pH is about 7. The degradation rate of SPD would be higher with the increase of water velocity in the WDS. Different pipe materials were studied and the degradation effect of SPD was in the following order: stainless-steel pipe > ductile iron pipe > polyethylene (PE) pipe. There was a significant increase in the content of trihalomethanes (THMs) when chlorination of tap water containing SPD in both the WDS and the beakers system were performed. The mechanism of this process was analyzed in combination with the intermediate products degraded by SPD in the process. In addition, density functional theory (DFT) calculations were performed to assist in the identification of 6 intermediates and possible degradation pathways.
研究领域:
环境科学
·
化学
·
生物学
✓ 已验证
DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2016
·
IF 3.13
Yang Lu,Biao Cong,Zhenjiang Tan,Yongsheng Yan
Polyoxyethylene lauryl ether (POELE10)-Na 2 C 4 H 4 O 6 aqueous two-phase extraction system (ATPES) is a novel and green pretreatment technique to trace samples. ATPES coupled with high-performance liquid chromatography (HPLC) is used to analyze synchronously sulfadiazine (SDZ) and sulfamethazine (SMT) in animal by-products (i.e., egg and milk) and environmental water sample. It was found that the extraction efficiency (E%) and the enrichment factor (F) of SDZ and SMT were influenced by the types of salts, the concentration of salt, the concentration of POELE10 and the temperature. The orthogonal experimental design (OED) was adopted in the multi-factor experiment to determine the optimized conditions. The final optimal condition was as following: the concentration of POELE10 is 0.027 g mL −1 , the concentration of Na 2 C 4 H 4 O 6 is 0.180 g mL −1 and the temperature is 35 °C. This POELE10-Na 2 C 4 H 4 O 6 ATPS was applied to separate and enrich SDZ and SMT in real samples (i.e., water, egg and milk) under the optimal conditions, and it was found that the recovery of SDZ and SMT was 96.20–99.52% with RSD of 0.35–3.41%. The limit of detection (LOD) of this method for the SDZ and SMT in spiked samples was 2.52–3.64 pg mL −1 , and the limit of quantitation (LOQ) of this method for the SDZ and SMT in spiked samples was 8.41–12.15 pg mL −1 .
研究领域:
分析化学
·
环境科学
·
食品科学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2021
·
IF 10.588
Shaona Wang,Rongfang Yuan,Huilun Chen,Fei Wang,Beihai Zhou
Large amounts of sulfanilamide antibiotics (SAs) have been excreted into the manure. In this study, the anaerobic biodegradation of four kinds of SAs including sulfaquinoxaline (SQX), sulfamethoxazole (SMX), sulfamethoxine (SMD) and sulfathiazole (STZ) was investigated. The degradation rates of SQX and STZ decreased with the increase of the concentrations of other organics, but those of SMX and SMD were less affected. The average degradation rates of SAs were in the order of SMX >SMD ≈QX >STZ, with the best degradation rate constants of 0.30125, 0.14752, 0.16696, and 0.06577 /d, respectively. STZ had the greatest effect on the population richness of microbes, whereas SQX had the largest impact on the population diversity. The degradation rates of SAs were positively correlated with the abundances of Proteobacteria and Bacteroidetes , and negatively correlated with the abundance of Firmicutes . The common degradation pathways of SAs were S-N cleavage and substitution. The specific functional groups of SQX, SMX and SMD, including quinoxaline , isoxazole and pyrimidine rings, could be opened, but the thiazole ring of STZ was difficult to be decomposed. After the rings of the specific functional groups were opened, they would be further substituted or decomposed to be products with small molecules.
研究领域:
环境科学
·
微生物学
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
·
IF 8.2
Han Zhang,Siyu Deng,Luxiang Zhu,Yanan Liu
The ubiquitous presence of sulfonamides (SAs) in wastewater poses serious risks to human health and ecosystem safety. This study evaluated the performance of a falling film dielectric barrier discharge (DBD) system on the removal of five SAs, namely sulfamethoxazole (SMX), sulfisoxazole (SIZ), sulfathiazole (STZ), sulfadiazine (SDZ) and sulfamerazine (SMR). Removal efficiencies >99 % were observed for all target SAs within 30 min of treatment, with pseudo-first order rate constants varying between 0.17 and 0.27 min −1 . Superior removal efficiencies were achieved under acidic conditions compared to neutral and alkaline conditions. Using SMX as a model compound, mechanistic investigations revealed that the synergy of reactive oxygen species (ROS) and reactive nitrogen species (RNS) led to its efficient degradation, with peroxynitrites (ONOO − /ONOOH) and hydroxyl radical (OH ) playing pivotal roles. SMX degradation pathways encompassing nitration/nitrosation, hydroxylation, deamination, C S and S N bond cleavage were proposed. The toxicity evaluation results demonstrated that the solution toxicity diminished following the plasma treatment under specific conditions. In particular, the solution treated with air or oxygen discharge enhanced the growth of wheat seedlings, suggesting the potential for reusing plasma-treated wastewater in agriculture. This study enhances our understanding of the underlying mechanisms involved in the plasma degradation of SAs and reveals the significant potential of plasma technology as a sustainable approach for treating wastewater.
研究领域:
环境科学
·
废水处理
·
等离子体技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Wondimeneh Dubale Adane,Bhagwan Singh Chandravanshi,Merid Tessema
In this study, we have developed a novel, hypersensitive, and ultraselective electrochemical sensor containing thermally annealed gold–silver alloy nanoporous matrices (TA-Au-Ag-ANpM) integrated with f -MWCNTs-CPE and poly( l -serine) nanocomposites for the simultaneous detection of sulfathiazole (SFT) and sulfamethoxazole (SFM) residues in honey, beef, and egg samples. TA-Au-Ag-ANpM/ f -MWCNTs-CPE/poly( l -serine) was characterized using an extensive array of analytical (UV–Vis, FT-IR, XRD , SEM , and EDX), and electrochemical (EIS, CV and SWV) techniques. It exhibited outstanding performance over a wide linear range, from 4.0 pM to 490 μM for SFT and 4.0 pM to 520 μM for SFM, with picomolar detection and quantification limits (0.53 pM and 1.75 pM for SFT, 0.41 pM and 1.35 pM for SFM, respectively). The sensor demonstrated exceptional repeatability, reproducibility, and anti-interference capability, with percentage recovery of 95.6–102.4% in food samples and RSD below 5%. Therefore, the developed sensor is an ideal tool to address the current antibiotic residue crisis in food sources.
研究领域:
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL LETTERS
·
2020
·
IF 1.467
Xu Ning,Liu Mu-Hua,Yuan Hai-Chao,Huang Shuang-Gen,Wang Xiao,Zhao Jin-Hui,Chen Jian,Wang Ting,Hu Wei,Song Yi-Xin
In order to meet the growing demand for food safety, the rapid classification of sulfadimidine and sulfapyridine in duck meat based on surface-enhanced Raman spectroscopy (SERS) was investigated using gold nanoparticles (Au NPs) and NaCl solution as the active substrate and active agent, respectively. The results showed that the Raman characteristic peaks at 557 and 573 cm−1 were used to determine whether sulfadimidine and sulfapyridine remained in duck meat. A single factor experimental method was used to optimize the conditions. Three pretreatment methods were employed to optimize the raw measurements. According to the classification accuracies, adaptive iterative reweighted penalty least squares and second derivative were selected to be the optimal pretreatment method. Next, principal component analysis (PCA) was performed to extract the characteristic variables. The first four score values of PCA were selected to be the input values of support vector machine (SVM) classification model, which classified duck meat samples into four categories. Nu-support vector classification and radial basis function were selected to be the employed SVM classification model and the Kernel type, respectively. The gamma and nu values were 0.25 and 0.255, respectively. The classification accuracy for the test set was 90.44%. The sensitivities and specificities of test set were calculated, and the highest sensitivity and specificity values were 96.97% and 100%, respectively. The experimental results showed that this classification model provides favorable classification results. Therefore, the adopted method may be used for the rapid classification of sulfadimidine and sulfapyridine residues in duck meat.
研究领域:
食品安全
·
光谱学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Liye Wang,Jiangxiu Niu,Pengyuan Wei,Yilei Feng,Mingyue Ding,Chaojun He,Yongjie Ma,Yaolei Zhu,Jia Li,Lingli Huang,Xiya Zhang
To reduce matrix interference and realize simultaneous detection of multiple homologous compounds (trimethoprim (TMP), diaveridine (DVD), ormetoprim (OMP), baquiloprim (BQP), and aditoprim (ADP) in pig, cattle, chicken, and fish muscles), an immunomagnetic bead (IMB)-based sample purification pretreatment with HPLC–UV was developed. A broad-spectrum monoclonal antibody (mAb, named 14C6) was prepared and conjugated with carboxylic-acid-functionalized magnetic nanoparticles using the active ester method to obtain IMBs for sample purification. The extraction solvent was optimized based on the extraction efficiency. Good linearity was observed for all the five analytes (10–200 μg/kg) with the LOD and LOQ of 5 and 10 μg/kg, respectively. The mean recoveries ranged from 62.5% to 76.9%, while the coefficient of variation was <12.2%. The IMB method afforded greater sample purification and enrichment than those achieved with the SPE column-based conventional method. Hence, the IMB-based sample purification is a useful tool to determine 2,4-diaminopyrimidine residues in edible animal tissues.
研究领域:
分析化学
·
食品安全
·
兽医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
·
IF 8.2
Xueqin Yang,Yijing Shi,Guangguo Ying,Mengyuan Li,Zhili He,Longfei Shu
Ammonia-oxidizing microorganisms, including AOA (ammonia-oxidizing archaea), AOB (ammonia-oxidizing bacteria), and Comammox (complete ammonia oxidization) Nitrospira , have been reported to possess the capability for the biotransformation of sulfonamide antibiotics. However, given that nitrifying microorganisms coexist and operate as communities in the nitrification process, it is surprising that there is a scarcity of studies investigating how their interactions would affect the biotransformation of sulfonamide antibiotics. This study aims to investigate the sulfamonomethoxine (SMM) removal efficiency and mechanisms among pure cultures of phylogenetically distinct nitrifiers and their combinations. Our findings revealed that AOA demonstrated the highest SMM removal efficiency and rate among the pure cultures, followed by Comammox Nitrospira , NOB, and AOB. However, the biotransformation of SMM by AOA N. gargensis is reversible, and the removal efficiency significantly decreased from 63.84 % at 167 h to 26.41 % at 807 h. On the contrary, the co-culture of AOA and NOB demonstrated enhanced and irreversible SMM removal efficiency compared to AOA alone. Furthermore, the presence of NOB altered the SMM biotransformation of AOA by metabolizing TP202 differently, possibly resulting from reduced nitrite accumulation. This study offers novel insights into the potential application of nitrifying communities for the removal of sulfonamide antibiotics (SAs) in engineered ecosystems.
研究领域:
环境生物学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）