ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Binge Deng,Linley Li Lin,Yan Wang,Xiangdong Bu,Jin Li,Jingsong Lu,Yaohui Wang,Yao Chen,Jian Ye
The use of dual-tracer contrast agents in clinical applications, such as sentinel lymph node (SLN) identification, offers significant advantages including enhanced accuracy, sensitivity, as well as comprehensive and multimodal visualization. In the current clinical practice, SLNs are typically marked prior to surgical resection by multiple and sequential injections of two tracers, the radioactive tracer and methylene blue (MB) dye. This imposes physical and psychological burden on patients and medical staff. Surface-enhanced Raman scattering (SERS) nanotags have emerged as promising SLN tracers due to their high sensitivity and specificity. In this study, we propose a novel single-injection composite tracer consisting of SERS nanotags and MB dye solution, to achieve the accurate intraoperative visualization and localization of SLNs. Laser excitation at the second near-infrared window (1064 nm) minimizes the MB fluorescence background interference, allowing the integration of SERS nanotags with MB solution to form the composite tracer, bridging two distinctive but complementary optical modalities. The feasibility of the composite tracer is demonstrated for SLN navigation on rabbit models. For the first time, we successfully visualize and localize multiple SLNs in the axilla of rhesus monkeys. Our study demonstrates the potential of combining MB with SERS nanotags for SLN navigation as the composite tracer, making a significant advancement toward the SLN biopsy in clinical applications.
研究领域:
医学影像学
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肿瘤学
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生物医学工程
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DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2024
·Vol. 48
·期 16
·7300-7307
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IF 2.7
Chunxiong Lu,Dandan Zhu,Peng Zhou,Kangxia Yu,Yaling Liu,Hongyong Wang,Hao Wu,Jun Wu,Guoqing Han,Pei Zou
Enormous success has been achieved in antitumor therapy with PD-1/PD-L1 inhibitors; however, less than 3 out of 10 patients show clinical benefits. An interesting phenomenon of patients who experienced durable clinical responses to PD-L1 blockade usually being the patients with high PD-L1 expression was noticed. Accordingly, the evaluation of PD-L1 is of great benefit to predict the immunotherapy response against PD-1/PD-L1. A small-molecule single photon emission computed tomography (SPECT) tracer [99mTc]Tc-SG2C-CBM containing a biphenyl moiety was designed and synthesized for the measurement of PD-L1 expression. The radiolabeling yield (RLY) and radiochemical purity (RCP) of [99mTc]Tc-SG2C-CBM were detected to be over 93% under the optimized labeling conditions. [99mTc]Tc-SG2C-CBM was discovered to be equipped with not only good stability but also suitable lipophilicity. In the cellular assays, [99mTc]Tc-SG2C-CBM showed moderate affinity to PD-L1 and 6.87-fold higher uptake in A375-hPD-L1 cells than in A375 cells. Then, the in vivo biodistribution results of the A375-hPD-L1 tumor-bearing mice showed that the radioactivity uptake in tumors was about 2.96 ± 0.76 and 2.31 ± 0.26 fold that in muscles and bones at 2 h post-injection, respectively. SPECT imaging was consistent with the biological distribution of [99mTc]Tc SG2C-CBM, with the uptake being mainly in the liver and less pronounced in tumors. The results of these studies indicate that [99mTc]Tc-SG2C-CBM may be a probe for imaging PD-L1 expression, but further research is needed.
研究领域:
医学影像学
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肿瘤学
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药物化学
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DOI
高影响因子
JOURNAL OF MEDICINAL CHEMISTRY
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2024
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IF 6.8
Zuojie Li,Xiaojiang Duan,Peiwen Han,Guangxing Yin,Yuhao Jiang,Qing Ruan,Junbo Zhang
The development of novel tracers targeting prostate-specific membrane antigen (PSMA) has great potential for improving the diagnosis and treatment of prostate cancer (PCa). This study aimed to improve the absolute tumor uptake and tumor-to-background ratios (TBRs) of this novel PSMA tracer by increasing the number of pharmacophores, Glu-urea-Lys (EuK), that specifically bind to PSMA. We successfully synthesized four radioligands and prepared a total of 12 stable radiotracers by coordinating 99mTc with various coligands. [99mTc]Tc-EUKD-EDDA showed the best pharmacokinetic properties both in vitro and in vivo. It effectively increased the absolute uptake in tumors and resulted in good tumor retention. Rapid clearance in nontarget organs resulted in high TBRs. High-contrast SPECT/CT images were obtained within 2–6 h after injection, suggesting that [99mTc]Tc-EUKD-EDDA has great application potential in time-lapse imaging of PCa, which is important for improving the diagnostic accuracy of PCa in clinical practice.
研究领域:
医学影像学
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核医学
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肿瘤学
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DOI
PubMed
高影响因子
JOURNAL OF CLINICAL INVESTIGATION
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2025
·Vol. 135
·期 6
·
IF 13.6
Lei Xia,Yan Wu,Yanan Ren,Zhen Wang,Nina Zhou,Wenyuan Zhou,Lixin Zhou,Ling Jia,Chengxue He,Xiangxi Meng,Hua Zhu,Zhi Yang
BACKGROUND. B7H3, also known as CD276, is notably overexpressed in various malignant tumor cells in humans, with extremely high expression rates. The development of a radiotracer that targets B7H3 may provide a universal tumor-specific imaging agent and allow the noninvasive assessment of the whole-body distribution of B7H3-expressing lesions.
METHODS. We enhanced and optimized the structure of an affibody (ABY) that targets B7H3 to create the radiolabeled radiotracer [68Ga]Ga-B7H3-BCH, and then, we conducted both foundational experiments and clinical translational studies.
RESULTS. [68Ga]Ga-B7H3-BCH exhibited high affinity (equilibrium dissociation constant [KD] = 4.5 nM), and it was taken up in large amounts by B7H3-transfected cells (A549CD276 and H1975CD276 cells); these phenomena were inhibited by unlabeled precursors. Moreover, PET imaging of multiple xenograft models revealed extensive [68Ga]Ga-B7H3-BCH uptake by tumors. In a clinical study including 20 patients with malignant tumors, the [68Ga]Ga-B7H3-BCH signal aggregated in both primary and metastatic lesions, surpassing fluorine-18 fluorodeoxyglucose (18F-FDG) in overall diagnostic efficacy for tumors (85.0% vs. 81.7%), including differentiated hepatocellular and metastatic gastric cancers. A strong correlation between B7H3 expression and [68Ga]Ga-B7H3-BCH uptake in tumors was observed, and B7H3 expression was detected with 84.38% sensitivity and 100% specificity when a maximum standardized uptake value (SUVmax) of 3.85 was set as the cutoff value. Additionally, B7H3-specific PET imaging is expected to predict B7H3 expression levels in tumor cells, intratumoral stroma, and peritumoral tissues.
CONCLUSION. In summary, [68Ga]Ga-B7H3-BCH has potential for the noninvasive identification of B7H3 expression in systemic lesions in patients with malignant tumors. This agent has prospects for improving pretreatment evaluation, predicting therapeutic responses, and monitoring resistance to therapy in patients with malignancies.
TRIAL REGISTRATION. ClinicalTrials.gov NCT06454955.
FUNDING. This research was financially supported by the Natural Science Foundation of Beijing Municipality (no. 7242266), the National Natural Science Foundation of China (no. 82202201), and the Young Elite Scientists Sponsorship Program by China Association for Science and Technology (CAST) (no. YESS20220230).
研究领域:
肿瘤学
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分子成像
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核医学
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诊断学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
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2025
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IF 4.7
Lican Wang,Weikun Zhu,Jie Li,Junyi Yue,Jiangyao Chen
H 2 O shows a significant contribution to form oxygen- and hydrogen-containing products during photocatalytic oxidation of aromatic hydrocarbons. However, it was unclear what the roles of atoms in H 2 O in subsequent oxidation of these products. In this study, the photocatalytic oxidation of two aromatic alcohols, benzyl alcohol and phenethyl alcohol, on TiO 2 (79 % anatase and 21 % rutile) were investigated in mixed H 2 O (or H 2 18 O, D 2 O) and O 2 system. Aromatic aldehydes, acids and esters were main aromatic products, while modified TiO 2 and isotope-containing H 2 O did not change the product species. Aromatic aldehydes showed ≤ 0.19 % and ≤ 1.58 % of 18 O- and D-labeling rates, suggesting hardly involvement of H 2 O in their formation. Aromatic acids exhibited a 18 O-labeling rate range of 28.12 %-40.33 % and the highest D-labeling rate of 0.76 %, while a maximum enhancement of 18 O-labeling rate was 0.11 % in the –C=O group of the acids, indicating that H 2 O selectively provided O atom in the formation of –OH group of aromatic acids. The 18 O- and D-labeling rates of aromatic esters were in the range of 9.01 % to 15.95 % and 1.73 % to 8.08 %, suggesting that both oxygen and hydrogen atoms from H 2 O contributed to aromatic ester formation. Further analyzing mass fragmentation confirmed that H 2 O separately contributed its hydrogen and oxygen atoms to the –C=O and R–O groups of the esters. Based on the isotope tracing results, the contribution roles of H 2 O’s hydrogen and oxygen atoms in oxidation of aromatic alcohols to corresponding aromatic products were proposed. The findings in this study help to clarify the role of H 2 O in the oxidation process of aromatic alcohols at the atomic level and comprehensively reveal the oxidation mechanism of the aromatics.
研究领域:
环境科学
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化学
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2023
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IF 15.1
Fan Feng,Caiyan Qu,Jia Tang,Xing Wu,Xi Tang,Fubing Yao,Liyuan Chai,Ruiyang Xiao,Chong-Jian Tang
Fe and S could improve the anammox performance by coupling with the N cycle. However, the coexistence of Fe and S complicates the metabolic process, troubling the quantification of nitrogen removal pathways. Herein, the nitrogen removal pathways were quantificationally analyzed by 15 N stable isotope tracing and elaborate batch tests, and the enhancing mechanism was illuminated by physiological and metatranscriptome analyses in pyrite (FeS 2 )-amended anammox system. We found that anammox, denitrification and Feammox (NH 4 + oxidation coupled with Fe(III) reduction) contributed to 80.22 %, 19.53 % and 0.25 % nitrogen removal in this process. With pyrite addition, Fe, S release and the extracellular polymeric substances secretion were responsible for the enhancement of nitrite and nitrate removal via chemical, autotrophic and heterotrophic denitrification, improving nitrogen removal by 14 % and 19 %. The network analyses further confirmed that the multiple nitrogen removal pathways (i.e., anammox, Feammox and denitrification) contribute to pyrite-induced nitrogen removal enhancement in the anammox system. This work enables the in-depth understanding, future design and optimization of the pyrite-amended anammox system, providing a fundamental basis for developing Fe- and S-based anammox enhancement technologies .
研究领域:
环境生物学
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微生物学
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DOI
高影响因子
DESALINATION
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2025
·
IF 9.8
Hengtong Xia,Xiaoxin Hu,Wulong Zhang,Rumeng Du,Zhihao Chen,Hui Xu,Xiaoshuang Yin,Ying Liu,Yan Zhang,Wenzhong Yang
In high-temperature industrial water systems, developing environmentally friendly and efficient water treatment agents is crucial for inhibiting mineral scale formation and microbial contamination. In this study, a novel multifunctional water treatment agent, PASP-SQDs, was synthesized by modifying sulfur quantum dots (SQDs) with polyaspartic acid (PASP), endowing it with high-efficiency scale inhibition, antibacterial properties, and fluorescent tracing capabilities. The microstructure and spectral characteristics of PASP-SQDs were analyzed using X-ray photoelectron spectroscopy, high-resolution transmission electron microscopy, ultraviolet-visible spectroscopy, and fluorescence spectrophotometry. Notably, PASP-SQDs exhibited excellent fluorescence properties, enabling their use as a tracer for accurate dosing and real-time concentration monitoring of water treatment agents. This feature significantly enhances their practical applicability, allowing for intelligent and efficient water quality management. Static scale inhibition experiments were conducted to evaluate the inhibitory effects of PASP-SQDs on calcium carbonate (CaCO 3 ) and calcium sulfate (CaSO 4 ) scale formation, along with assessing their antibacterial performance. The results showed that PASP-SQDs significantly outperformed PASP alone, achieving scale inhibition rates of nearly 90 % for CaCO 3 (at 25 mg/L) and 98 % for CaSO 4 (at 0.6 mg/L). Additionally, at a concentration of 180 mg/L, PASP-SQDs exhibited an antibacterial efficiency exceeding 99 %, effectively preventing microbial contamination in water systems. The scale inhibition mechanism of PASP-SQDs was further investigated using X-ray diffraction, scanning electron microscopy, molecular dynamics simulations, and quantum chemical calculations. This study provides a comprehensive understanding of the working mechanism of PASP-SQDs, laying a solid theoretical and practical foundation for the development of next-generation, multifunctional, eco-friendly, and highly efficient water treatment agents.
研究领域:
环境科学
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材料科学
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工业水处理
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产品关联
DOI
高影响因子
FUEL
·
2024
·
IF 6.7
Xiao-Dong Zhou,Xue-Long Yin,Lang Liu,Xue-Li Huang,Jing-Mei Liu,Ting Liu,He Lin,Feng-Yun Ma
Understanding hydrogen activation is crucial for improving the performance of direct coal liquefaction (DCL) which is essential for the clean, efficient conversion of coal to fuel oils and aromatic chemicals. Three hydrogen sources are involved in DCL: solvent hydrogen (SH), dissolved hydrogen that reacts directly (DHD), and dissolved hydrogen that reacts through a solvent (DHS). Quantitatively studying DHS is challenging because it is generated through the dehydrogenation of hydrogen-donor solvents produced via the hydrogenation of gas-phase H 2 and solvents. This research establishes for the first time a quantitative method for determining DHS consumption (DHSC) based on protium- and deuterium-nuclear-magnetic-resonance results for solvents after isotope-tracer reactions. Comparative experiments and quantum-chemical calculations were performed to confirm the method’s reliability. The isotope-tracer method showed that DHSC accounts for < 7 % of the total hydrogen consumption under catalysis with ferric stearate, nickel stearate, or molybdenum 2-ethylhexanoate, while DHD consumption accounts for > 50 %. Thus, DHD, rather than DHS, is the primary hydrogen source for catalytic activation. Furthermore, the comparative experiments also showed that hydrogen consumption is greater for one hydrogen source than for the coexistence of the three hydrogen sources, indicating competition among the three sources. The quantum-chemical calculations showed that the competitiveness among the three sources follows the order of DHS < SH < DHD, this agrees with the order of hydrogen consumption in the isotope-tracer experiments. This study quantitatively reveals the mechanism responsible for hydrogen activation by catalysts and provides a scientific basis for optimization and mutual matching of solvents and catalysts.
研究领域:
化学工程
·
能源科学
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产品关联
DOI
高影响因子
Petroleum Science
·
2024
·
IF 6
Fang-Jun Jia,Hai-Tao Li,Zi-Min Liu,Xin Ma,Guo-Qiang Zhang,Qi-Hui Zhang,Jie Li
As an efficient monitoring and prediction tool, chemical tracers have been widely applied in reservoir characterization, production monitoring, water resources monitoring, and various other fields. Chemical tracer technology is characterized by high efficiency, high precision, relatively simple operational procedures, and low cost. Owing to the limitations of existing tracers, such as minimal options, limited transport efficiency, and complex detection methods, this study used fluorescein isothiocyanate and a ruthenium complex (Ru(phen 3 ) 2+ ) to synthesize 50 nm multi-color fluorescent silica nanoparticle tracers using an improved Stöber method based on fluorescence resonance energy transfer (FRET). Due to the FRET between the two compounds, the synthesized tracer exhibited the characteristics of multi-color fluorescence, and its fluorescent color varied with the mixing ratio of the two precursor solutions. The fluorescence intensity of the synthesized tracer was significantly higher than that of the monochromatic fluorescent nano-tracer. Fourier-transform infrared spectroscopy, ultraviolet spectrophotometry, and fluorescence spectrometry were used to characterize the structure, maximum absorption wavelength, and fluorescence characteristics of the synthesized tracer, respectively. The experimental results show that the synthesized tracer has a maximum absorption wavelength of 450 nm and an emission wavelength of 576 nm. Under field emission scanning electron microscopy, the tracer appears as uniformly spherical particles with a size of 50 nm (±5). It exhibited good dispersibility and fluorescence characteristics in reservoir environments that varied in temperature (25∼85 °C) and salinity (1000∼10000 mg/L). The effects of environmentally sensitive clay minerals, tracer particle size, injection concentration, fluid salinity, and flow rate on the transport characteristics (retention) of tracers in sandstone cores were studied using 12 sets of tracer breakthrough experiments. The experimental results showed that increased sensitivity to clay minerals, salinity, and tracer particle size were not conducive to tracer migration in the core. In contrast, increased tracer injection concentration and flow rate were beneficial to tracer migration in the core.
研究领域:
化学工程
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石油工程
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材料科学
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Mingjin Du,Channa Wang,Yilin Zhu,Chao Ma,Jiangtao Wang,Gang Chang,Mengyu Sun,Tongsiliu Wu,Yu Xiao,Zheng Zhao,Junwei Su,Xiangdong Ding,Changsheng Xiang
Oilfield tracers are essential chemical additives in the petroleum extraction process, aiding in understanding reservoir flow characteristics, optimizing production strategies, and ultimately increasing oil recovery rates. Carbon dots (CDs), known for their tunable photoluminescent properties, ease of detection, and low toxicity, are considered promising candidates for the next generation of oilfield tracers. However, aqueous-phase oilfield tracers require CDs to possess good water solubility, high-temperature and high-salinity tolerance, low rock adsorption and detection limit, which renders many CDs unqualified for use. Currently, reports on CDs oilfield tracers are limited, with most being restricted to blue fluorescence, which has weak resistance to background fluorescence interference. In this work, by utilizing the rich organic content (17.5 wt%) of low-value lignite as a precursor, lignite-derived carbonized polymer dots (L-CPDs) exhibit green fluorescence with an absolute quantum yield (QY) of 19.78%. Comprehensive evaluations show L-CPDs offer good salt tolerance, thermal stability, and pH stability, with a detection limit reaching 1 ppb and no significant cytotoxicity. Additionally, the tracing behavior of L-CPDs is demonstrated using a microfluidic chip, highlighting their potential as alternatives to existing tracer products.
研究领域:
石油工程
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材料科学
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环境科学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
·
IF 8.2
Yeshun Zhang,Xinyu Cao,Jingya Zhang,Guozheng Zhang,Mingkun Zhu,Hui Yan,Yurong Li
Hydrogel systems with strong fluorescence, as convenient tracers or bio-probes, have attracted much attention in biomedical engineering. Currently, most hydrogels endowed fluorescent properties due to modifying additional fluorophores. However, these fluorophores owing to photobleaching and toxicity limit the practical applications of hydrogels. Herein, we prepared a novel self-luminescence hydrogel through double crosslinking glutaraldehyde and hydrogen peroxide/horseradish peroxidase (H 2 O 2 /HRP) with sericin protein. The double cross-linked sericin hydrogel exhibits strong green and red intrinsic fluorescence which can be excited over a wide range of wavelengths. Moreover, this hydrogel with strong intrinsic fluorescence could penetrate thick pigskin tissue, which has potential application in implantable bio-tracer areas. In addition to the above unique properties, this sericin hydrogel possesses two types of micropore structures with high porosity, swelling properties, pH-responsive degradability, super elasticity, injectability, viscosity, and excellent biocompatibility. The investigation could significantly expand the scope of protein hydrogels in biomedical applications.
研究领域:
生物医学工程
·
材料科学
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DOI
高影响因子
CHEMOSPHERE
·
2024
·
IF 8.1
Lingchao Kong,Yi Wang,Dongyu Cui,Wei He,Chuanlun Zhang,Chunmiao Zheng
Antimicrobial resistance (AMR) in oceans poses a significant threat to human health through the seafood supply chain. Ammonia-oxidizing archaea (AOA) are important marine microorganisms and play a key role in the biogeochemical nitrogen cycle around the world. However, the AMR of marine AOA to aquicultural antibiotics is poorly explored. Here, Raman-deuterium isotope probing (Raman-DIP), a single-cell tool, was developed to reveal the AMR of a typical marine species of AOA, Nitrosopumilus maritimus (designated SCM1), against six antibiotics, including erythromycin , tetracycline , novobiocin , neomycin , bacitracin , and vancomycin . The D 2 O concentration (30% v/v) and culture period (9 days) were optimized for the precise detection of metabolic activity in SCM1 cells through Raman-DIP. The relative metabolic activity of SCM1 upon exposure to antibiotics was semi-quantitatively calculated based on single-cell Raman spectra. SCM1 exhibited high resistance to erythromycin, tetracycline, novobiocin, neomycin , and vancomycin , with minimum inhibitory concentration (MIC) values between 100 and 400 mg/L, while SCM1 is very sensitive to bacitracin (MIC: 0.8 mg/L). Notably, SCM1 cells were completely inactive under the metabolic activity minimum inhibitory concentration conditions (MA-MIC: 1.6–800 mg/L) for the six antibiotics. Further genomic analysis revealed the antibiotic resistance genes (ARGs) of SCM1, including 14 types categorized into 33 subtypes. This work increases our knowledge of the AMR of marine AOA by linking the resistant phenome to the genome, contributing to the risk assessment of AMR in the underexplored ocean environment. As antibiotic resistance in marine microorganisms is significantly affected by the concentration of antibiotics in coastal environments, we encourage more studies concentrating on both the phenotypic and genotypic antibiotic resistance of marine archaea . This may facilitate a comprehensive evaluation of the capacity of marine microorganisms to spread AMR and the implementation of suitable control measures to protect environmental safety and human health.
研究领域:
微生物学
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环境科学
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海洋生物学
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DOI
PubMed
高影响因子
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