Journal of Oleo Science
·
2023
·
IF 1.5
Ximei Yang,Sen Lin,Ying Chen,Wen Chen,Dongming Lan,Yonghua Wang
Nervonic acid (NA) is a monounsaturated fatty acid vital for brain health and is of emerging importance in various industrial applications, including therapeutics, food, and cosmetics. Given the growing demands of the food and pharmaceutical industries, there's a pressing need for high-purity NA. Previously, NA constituents in plant seed oils were chemically transformed into nervonic acid ethyl ester (NAEE) to facilitate extraction from seed oils. In this study, we present an enzymatic approach to convert NA constituents in Malania oleifera seed oil to NAEE. Combined with the utilization of the semi-preparative chromatography, we achieved a remarkable purity of 97.52% NAEE. Compared to conventional chemical preparations characterized by multiple steps, prolonged processing times, and low yields and purities, our enzymatic method stands out as a more efficient and advantageous alternative. On top of that, this innovative approach is environmentally friendly and circumvents health and safety issues associated with chemical processes.
研究领域:
生物化学
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食品科学
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制药工程
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产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF CHEMICAL ENGINEERING
·
2025
·
IF 3.7
Yingxi Gao,Tong Wei,Jie Wang,Yuming Tu,Zhiyong Zhou,Chencan Du,Zhongqi Ren
Nervonic acid (NA) is a long-chain monounsaturated fatty acid with significant potential for neural fiber repair. In this study, a mixed fatty acid methyl ester was synthesized as the raw material through saponification of Acer truncatum Bunge seed oil. Based on the differences in boiling points and relative volatilities of various components, a four-stage vacuum batch distillation process was employed to enrich the nervonic acid methyl ester (NAME). The effect of distillation process parameters on enrichment efficiency was investigated, including distillation temperature, operating pressure, and reflux ratio. The purity of NAME achieved as 91.20% under optimal conditions and the corresponding yield was 48.91%. To further increase the purity, a low-temperature crystallization process was adopted and a final purity of NAME was obtained as 97.56%. Simulation of the above four-stage batch distillation was conducted using Aspen Plus software, and a continuous distillation processes was further simulated to establish a theoretical basis for future industrial-scale production. The results of experiments and simulation demonstrate that the integrated process of vacuum distillation and low-temperature crystallization exhibits remarkable separation performances, providing robust guidance for the production of high-purity NA.
研究领域:
生物化学
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食品科学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
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2025
·
IF 9.8
Chenyu Jiang,Xinghe Zhang,Jiahui Yu,Ling-zhi Cheong,Xingguo Wang,Wei Wei
Herein, polar lipids, including glycerophospholipid, lysophospholipid, cholesterol (CHOL), sphingomyelin (SM), and ceramide (Cer), in human breast milk were subjected to lipidomic analysis via UPSFC-Q-TOF-MS. A total of 11 lipid classes and 195 molecular species were identified and quantified in human breast milk samples ( n = 126) from three lactational stages across three regions in China. CHOL and SM together account for more than 50 % of the total polar lipids. The total polar lipids, SM, and phosphatidylethanolamine (PE) were ∼ 54.5, ∼17.8, and ∼ 3.5 mg/100 mL, respectively, which both significantly ( P < 0.05) increased from colostrum to later lactational stages. DHA is highly enriched (68 % of the total DHA) in PE molecules. SM and Cer contain high ratios of nervonic acid (24:1 n-9), ∼35 % and ∼ 23 %, respectively. General patterns exist in the variation of polar lipid molecular species in human breast milk across different regions throughout the lactational stages.
研究领域:
营养学
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脂质组学
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生物化学
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产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF CHEMICAL ENGINEERING
·
2022
·
IF 3.898
Yingxi Gao,Jiayi Shi,Jie Wang,Fan Zhang,Shichao Tian,Zhiyong Zhou,Zhongqi Ren
Nervonic acid is the world's first and only potent substance that can repair damaged nerve fibers and promote nerve cell regeneration with high nutritional value. The wide variety of fatty acids in plant oils and fats with similar structures makes the large-scale separation and purification of high-purity nervonic acid very difficult. A new combined process of molecular distillation, urea inclusion and solvent crystallization was established to prepare high-purity nervonic acid with the mixed fatty acids obtained after saponification and acidification of Acer truncatum Bunge oil as raw materials. First, according to the difference in the mean free path of fatty acids, molecular distillation was used to separate and remove C16 saturated fatty acid of palmitic acid and four C18–C20 fatty acids of stearic, oleic, linoleic, and linolenic acids. The content of C16–C20 fatty acids decreased from 72.92% to 19.22% after two-stage molecular distillation processes, in which the contents of saturated fatty acid of palmitic acid decreased to about 0.5%. Then, according to the difference in carbon chain length and saturation of fatty acid, the contents of C22–C24 saturated fatty acids of tetracosanoic and docosanoic acids decreased to 0.21% and 0.07% by urea inclusion with urea/free fatty acid preparation by saponification (SPOMFs) ratio as 0.6. In addition, all saturated fatty acids were basically separated. Finally, according to the difference in the solubility of fatty acids in solvents, the C18–C20 unsaturated fatty acids of oleic, linoleic, and linolenic acids and C22 unsaturated fatty acid of erucic acid were removed by solvent crystallization. The content of C18–C20 unsaturated fatty acids decreased to less than 5% with pentanol as the solvent after the first stage solvent crystallization. The content of erucic acid decreased to 3.47% with anhydrous ethanol as the solvent after the second to fifth stage solvent crystallization. The combined process of molecular distillation, urea inclusion and low temperature crystallization innovatively adopted an efficient, simple and easy-to-industrial solvent crystallization method to separate erucic and nervonic acids, obtaining nervonic acid with purity of 96.53% and final yield of 47.99%.
研究领域:
食品科学
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生物化学
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DOI
高影响因子
Foods
·
2024
·Vol. 13
·期 17
·2757
·
IF 4.7
Hui Gao,Jie Sun,Xuan Guo,Ziyan Zhang,He Liu,Zhiran Zhang,Mengkai Liu,Sen Zhou,Shengxin Li,Tingting Zhang
Seven fatty acids were detected by GC-MS inXanthoceras sorbifoliumBunge seed oil extracted at different temperatures, including Palmitic acid C16:0, Stearic acid C18:0, Oleic acid C18:1, Eicosenoic acid C20:1, Docosenoic acid C22:1, Tetracosenoic acid C24:1, and Linoleic acid C18:2. The highest content of nervonic acid (NA) was found inXanthoceras sorbifoliumBunge seed oil extracted at 70 °C. Three methods were selected to analyze the extraction rate of nervonic acid inXanthoceras sorbifoliumBunge seed oil, including urea complexation, low-temperature solvent crystallization, and a combined treatment using these two methods. The final content of nervonic acid obtained was 14.07%, 19.66%, and 40.17%, respectively. The combined treatment method increased the purity of nervonic acid inXanthoceras sorbifoliumBunge seed oil by 12.62 times. Meanwhile, thermogravimetric behavior analysis of samples extracted using different methods was conducted by thermogravimetric analyzer, which suggested that the thermal stability of the samples extracted by the combined treatment was enhanced. These results can provide a new process parameter and scientific basis for the extraction of NA. At the same time, FTIR and NMR were also used to characterize the combined extraction sample, and the structure of the samples was proved.
研究领域:
植物学
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生物化学
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食品科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
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2021
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IF 6.165
Yu Jin,Feng Li,Xinman Lou,Yaqing Xiao,Xiaojing Wang,Fuguo Liu,Jun Wang,Huaide Xu
Nervonic acid (NA) has considerable potential for promoting brain development, but its utilization is limited attributed to its poor water solubility. Novel NA-loaded nanoemulsions (NA-NEs) were fabricated with planted-based soybean protein isolates (SPI), compared with whey protein isolates and Tween 20. The impact of emulsifiers on NA-NEs properties and stabilities was evaluated. Emulsions formulated with 0.5 mg/mL nervonic acid and 1.5 wt% SPI under pressure (120 MPa, 5 cycles) contained nano-scale droplets and considerable rheologic properties. The oil droplets were spherical and encapsulated within the emulsions, evidenced by atomic force microscope and confocal laser scanning microscopy. SPI-emulsions were stable under thermal treatment, NaCl concentrations, storage at 4 °C (30 days), and multiple scattering experimental conditions. SPI behaved similarly to the other two emulsifiers under the studied conditions. These findings demonstrate that SPI is a promising plant-based emulsifier for applications in nervonic acid-fortified food.
研究领域:
食品科学
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生物化学
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产品关联
DOI
高影响因子
ULTRASONICS SONOCHEMISTRY
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2016
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IF 4.556
Fengli Chen,Qiang Zhang,Shimin Fei,Huiyan Gu,Lei Yang
In this study, ultrasonic circulating extraction (UCE) technique was firstly and successfully applied for extraction of samara oil from Acer saccharum . The extraction kinetics were fitted and described, and the extraction mechanism was discussed. Through comparison, n -hexane was selected as the extraction solvent, the influence of solvent type on the responses was detailedly interpreted based on the influence of their properties on the occurrence and intensity of cavitation. Seven parameters potentially influencing the extraction yield of samara oil and content of nervonic acid, including ultrasound irradiation time, ultrasound irradiation power, ultrasound temperature, liquid-solid ratio, soaking time, particle size and stirring rate, were screened through Plackett–Burman design to determine the significant variables. Then, three parameters performed statistically significant, including liquid-solid ratio, ultrasound irradiation time and ultrasound irradiation power, were further optimized using Box–Behnken design to predict optimum extraction conditions. Satisfactory yield of samara oil (11.72 ± 0.38%) and content of nervonic acid (5.28 ± 0.18%) were achieved using the optimal conditions. 1% proportion of ethanol in extraction solvent, 120 °C of drying temperature and 6.4% moisture were selected and applied for effective extraction. There were no distinct differences in the physicochemical properties of samara oil obtained by UCE and Soxhlet extraction, and the samara oil obtained by UCE exhibited better antioxidant activities. Therefore, UCE method has enormous potential for efficient extraction of edible oil with high quality from plant materials.
研究领域:
食品科学与技术
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植物化学工程
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产品关联
DOI
PubMed
高影响因子
Advanced Composites and Hybrid Materials
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2022
·Vol. 5
·期 2
·1268-1279
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IF 11.806
Su Ji-Ling,Wang Juan,Fu Xiao-Na,Hou Li,Zhu Ping,Zhang Kai,Li Huo-Wang,Hou Ying,Liu Xiang-Yi,Xu Juan
This study explored the physicochemical properties, fatty acid compositions, and thermal characteristics of Malania oleifera seed oil (MOSO). Our data showed that the refractive index and specific gravity of MOSO at ambient temperature were 1.461 and 0.913, respectively. The oil content, acid, peroxide, saponification, iodine value, average molecular weight, and an average number of carbon–carbon double bond per triglyceride were 47.8%, 3.62 mg KOH/g oil, 4.61 meq O 2 /kg oil, 163.16 mg KOH/g oil, 104.28 g I 2 /100 g oil, 1031.51 g/mol, and 4.24, respectively. The fatty acid profiles of the methyl esters showed the presence of 96.09% unsaturated fatty acids and 2.16% saturated fatty acids. Nervonic acid (NA) was the major component (45.04%). These results suggested that MOSO is an excellent source of essential fatty acids and NA and may be used as a potential NA resource of health supplements and medicines. Graphical abstract
研究领域:
植物学
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食品科学
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化学工程
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产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2016
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IF 2.711
Peng Hu,Xuebing Xu,Liangli Lucy Yu
This study obtained trans -free fats containing nervonic acid, a fatty acid with beneficial effects for mental health. Four fats containing 1.28–2.41% (w/w) nervonic acid were prepared by chemical or enzymatic interesterification of palm stearin (PS), Acer truncatum oil (ATO) and palm kernel oil (PKO). The chemical procedure was catalyzed using MeONa, whereas the enzymatic procedure was catalyzed with a 1,3–specific lipase. As a result, the interesterified fats showed a similar fatty acid profile to their corresponding physical blends measured by gas chromatography , but contained more nervonic acids at the sn -2 position, which was more conducive to metabolism and absorption. The content of tripalmitoylglycerol (PPP) and 1,3-dipalmitoyl-2-oleoyl glycerol (POP) or 1,2-dipalmitoyl-3-oleoyl glycerol (PPO) in the interesterified fats decreased significantly, resulting in improved plasticities. All interesterified fats showed a satisfactory content of tocopherols and oxidative stability. A combination of differential scanning calorimetry , X-ray diffraction spectroscopy and polarized light microscopy results suggested that the interesterified fats formed spherulitic needle-like crystals in the β′ form, while the physical blends formed dendritic crystals in the β form. These data indicated that the trans -free interesterified fats rich in nervonic acid may have a great potential in margarine applications.
研究领域:
食品科学
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营养学
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生物化学
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产品关联
DOI
高影响因子
Food & Function
·
2023
·
IF 6.1
Dekun Cheng,Zhangtie Wang,Xin Guo,Yiwen Guo,Yu Zhang,Yuanhui Zhao,Ruijie Liu,Ming Chang
Dietary nutritional support for special populations is an effective and feasible method to improve quality of life of patients and reduce medical pressure. The effects of ATSO on chemotherapy population were evaluated by a zebrafish model of demyelination. The results showed that 100 μg/mL ATSO reversed tail morphology damage, axon degeneration, touch response delay, ROS level upregulation and the expression of myelin basic protein decrease in chemotherapy-induced zebrafish. In addition, the expression of myelin markers (including sox10, krox20, pmp22) in oxaliplatin-induced cells was markedly reversed by ATSO and its active components (gondoic acid, erucic acid, nervonic acid). ATSO and its active components could reverse demyelination by ameliorating mitochondrial dysfunction. Conversely, linoleic acid and linolenic acid promoted demyelination by exacerbating mitochondrial dysfunction. Moreover, the Pink1/Parkin pathway was recognized as the main reason for ATSO and its active components to improve mitochondrial function by activating mitophagy and restoring autophagic flow. Taken together, this study demonstrated that ATSO and its active components could be further developed as novel functional food ingredients to antagonize demyelination.
研究领域:
神经科学
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药理学
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营养学
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细胞生物学
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分子生物学
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DOI
PubMed
高影响因子
Food Bioscience
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2022
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IF 5.318
Yu Jin,Bin Shu,Xinman Lou,Kunhua Wang,Yunhui Zhai,Yingjuan Qu,Ruijuan Song,Fuguo Liu,Xiaobo Dong,Huaide Xu
Nervonic acid (NA, ω-9) is beneficial for brain health, but its poor water solubility limits its bioaccessibility. The protein-based emulsions were unstable under environmental stress. In this study, the effects of a nonionic surfactant (Tween 20, T20) on the characteristics, stability, and in vitro digestion properties of NA-loaded nanoemulsions prepared with proteins (whey or soybean protein isolate) were evaluated. The emulsions stabilized by protein-T20 mixtures had smaller droplets with more even distributions, more negative charges, and higher encapsulation efficiencies for NA. The addition of T20 improved the interfacial properties of the systems, as confirmed by spectral and rheological analyses. Emulsion stability under various conditions was enhanced by the improved interfacial composition and emulsifying ability. In particular, the effective interfacial layers produced by the protein-T20 mixture decreased lipid digestion but increased NA bioaccessibility. Therefore, the co-adsorption of proteins with suitable nonionic surfactants at oil-water interfaces is a promising approach for designing functional food delivery systems with improved stability and NA bioaccessibility.
研究领域:
食品科学
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营养学
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生物技术
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产品关联
DOI
高影响因子
MOLECULAR NUTRITION & FOOD RESEARCH
·
2024
·Vol. 68
·期 8
·2300671
·
IF 4.5
Hui Zhou,Ziyi Chen,Jingbin Li,Rongjin Wang,Hongshi Bu,Chengjiang Ruan
Scope Cerebral ischemia-reperfusion (IR) injury stands as a prominent global contributor to disability and mortality. Nervonic acid (NA), a bioactive elongated monounsaturated fatty acid, holds pivotal significance in human physiological well-being. This research aims to explore the prophylactic effects and fundamental mechanisms of NA in a rat model of cerebral IR injury. Methods and results Through the induction of middle cerebral artery occlusion, this study establishes a rat model of cerebral IR injury and comprehensively assesses the pharmacodynamic impacts of NA pretreatment. This evaluation involves behavioral analyses, histopathological examinations, and quantification of serum markers. Detailed mechanisms of nervonic acid's prophylactic effects are revealed through fecal metabolomics and 16S rRNA sequencing analyses. Our findings robustly support nervonic acid's capacity to ameliorate neurological impairments in rats afflicted with cerebral IR injury. Beyond its neurological benefits, NA demonstrates its potential by rectifying metabolic perturbations across diverse pathways, particularly those pertinent to unsaturated fatty acid metabolism. Additionally, NA emerges as a modulator of gut microbiota composition, notably by selectively enhancing vital genera like Lactobacillus . Conclusion These comprehensive findings highlight the potential of incorporating NA as a functional component in dietary interventions aimed at targeting cerebral IR injury.
研究领域:
神经科学
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药理学
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微生物学
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代谢组学
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病理学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）