Sensor Letters
·
2014
·Vol. 12
·期 10
·1462-1468
·
IF 0.558
Liu Zhi-Min,Zheng Ying-Ying,Jing Yan-Feng,Li Jie,Zhan Hai-Jun,Shen Qi
A facile, one-step synthesis of ionic liquid-functionalized graphene (IL-GR) nanocomposite using graphene oxide (GO) and amine-terminated ionic liquid (NH2-IL) was first presented through an epoxide ring-opening reaction, then acetylcholinesterase (AChE) was immobilized onto
IL-GR nanocomposite film modified glassy carbon electrode to prepare a highly sensitive AChE biosensor. The morphologies and electrochemistry properties of the composite were investigated by using scanning electron microscopy, transmission electron microscopy and cyclic voltammetry, respectively.
The nanocomposite not only provided a biocompatible microenvironment to keep the bioactivity of AChE, but also exhibited a strong synergetic effect on improving the sensing properties of phoxim. The immobilized AChE showed favorable affinity to acetylthiocholine (ATCl) and could catalyze the
hydrolysis of ATCl with a Kapp
m
value of 0.143 mM. The proposed biosensor exhibited a detection limit of 6.0 × 10–11 M (S/N = 3) towards phoxim. Besides, the biosensor is easy to prepare, have good stability, and will have potential application
in organophosphate pesticides.
研究领域:
分析化学
·
环境科学
·
生物技术
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Cunwei Wang,Man Peng,Xishun Peng,Haili Zhang,Caizhen Zu,Zhangwei Tan,Yuxue He,Zhongchen Bai
This study presents a surface-enhanced Raman scattering (SERS) sensing platform based on a MoS 2 /Au@Ag heterojunction for specifically detecting the residues of thiram pesticide. A Nano-MoS 2 film was firstly prepared by depositing MoS 2 nanosheets on the surface of a stainless steel sheet. Subsequently, Au nanoparticles and Ag nanoparticles were deposited on the surface of MoS 2 film to form a MoS 2 /Au@Ag heterojunction for SERS application. This well-performing SERS substrate was originated from the synergistic effect of electromagnetic (EM) enhancement and chemical (CM) enhancement to boost its SERS signal. Here, the CM contribution could be up to 41.8 % in the process of optimizing interfacial charge transfer (CT) pathways in MoS 2 /Au@Ag heterojunction. Thus, the SERS sensor based on MoS 2 /Au@Ag film showed an excellent signal homogeneity (RSD = 4.36 %) and an ultrahigh sensitivity to thiram molecules with a limit of detection (LOD) as low as 0.32 nM. Moreover, it could also recognize the thiram residues at 10 nM level in citrus peels, with a recovery rate of 91.0–109.0 %. The SERS sensor we developed in this study has a high sensitivity, excellent reproducibility, and long-term stability, which could be widely applied for the food safety monitoring
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Journal of Materials Chemistry B
·
2025
·
IF 5.7
Tingting Lei,Yating Xu,Yuanyu Tang,Siyi Tan,Jiafu Xiao,Shaojing Zhao,Minhuan Lan
Achieving highly selective visual detection of thiram is essential for ensuring food safety and environmental protection. Here, we developed a smartphone-integrated colorimetric and fluorometric dual-mode sensor based on copper-doped carbon dots (Cu-CDs). The Cu-CDs were synthesized via hydrothermal treatment of L-tryptophan and CuCl2·2H2O. These Cu-CDs exhibited two fluorescence emission peaks at 350 nm and 450 nm, with a fluorescence quantum yield of 20.8%. Cu2+ on the surface of the Cu-CDs could selectively bind to thiram to form a Cu2+-thiram complex, which quenched the fluorescence at 450 nm of Cu-CDs via the inner filter effect and dynamic quenching effect. In contrast, the fluorescence at 350 nm was changed slightly. The color of the solution was changed from colorless to yellow. These changes allowed for a fluorometric and colorimetric dual-mode optical response. The proposed sensor possessed high sensitivity and selectivity and demonstrated reliable performance in real water samples. Its integration with a smartphone further enabled convenient, on-site detection. This work presented a robust and cost-effective strategy for monitoring thiram in applications related to food safety and environmental protection.
研究领域:
分析化学
·
食品安全
·
环境监测
·
纳米生物技术
·
传感器开发
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CHINESE JOURNAL OF PHYSICS
·
2025
·
IF 4.6
Hanzhaobing Wu,Jin Xu,Hongping Zhao,Baolong Shi,Yu Wan,Hao Sun,Dalei Wang,Changwei Bi,Chao Wang,Wei Liu
Thiabendazole (TBZ) and thiram are widely used fungicides in agricultural production for effective disease control. However, their persistent environmental accumulation and migration lead to excessive residues in soil, groundwater, and other matrices, causing severe contamination and significant human health risks. In this study, TiO₂/PPy (polypyrrole) composites were synthesized via an eco-friendly chemical method to develop a photoelectrochemical (PEC) sensing platform for TBZ and thiram detection. The conductive PPy incorporation significantly enhanced TiO₂'s charge separation efficiency, yielding a notable photocurrent response (∼6 μA/cm²). The fabricated PEC sensor demonstrated reliable detection performance for both fungicides.
研究领域:
环境科学
·
材料科学
·
分析化学
·
传感技术
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Fang Yan,Rong Deng,Shaohui Guo,Xiaochun Li
Excessive pesticide residues have posed a severe risk to human health and ecological environment, making stringent testing of them a crucial step in food safety supervision. Herein, a flexible paper-based SERS platform composited of AgNPs and MoS 2 nanoflowers was developed for the rapid and ultra-sensitive detection of thiram in juice. Among it, the hydrophobic MoS 2 -modified paper was first fabricated, followed by co-depositing small-sized AgNPs and thiram molecules onto its surface for SERS analysis. Owing to the unique petal-like structure and high hydrophobicity of MoS 2 -coated paper, the AgNPs loaded on it was significantly aggregated, thereby generating numerous “hot spots” in the nanogaps between adjacent silver nanoparticles. Simultaneously, thiram molecules were enriched and concentrated within these gaps, resulting in exceptional Raman signal amplification. Compared to single-material substrates, an integrated advantage was acquired with hybridizing of AgNPs and MoS 2 . The performance of this platform was verified by detecting thiram in pear juice using a portable Raman spectrometer, in which the limit of detection (LOD) can be as low as 1.01 ng/mL and its recovery percentages had a good consistency with ones of HPLC-MS/MS. Overall, this Ag/MoS 2 hybrid SERS platform exhibits outstanding stability, sensitivity and feasibility in the label-free detection of pesticides in food. It also provided a universal tool for the on-site monitoring of diverse biochemical molecules across various fields.
研究领域:
分析化学
·
纳米技术
·
食品安全
·
光谱学
·
环境科学
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Wei Zhang,Kuang Luo,Wenjie Chen,Min Chen,Ning Cai,Jumei Li
In surface-enhanced Raman scattering (SERS) measurements, the creation of “hot spots” and the effective concentration of analytes in these areas are crucial for enhancing detection sensitivity. In this work, a novel composite with highly SERS activity, Ag nanospheres decorated Ag nanostar/ZIF-8 composite (Ag NS/ZIF-8/Ag) was achieved via first coating the Ag nanostars with the metal-organic framework ZIF-8, known for its strong molecular adsorption capabilities, followed by the electrostatic self-assembly of Ag nanospheres. The composites produce numerous hotspots due to the abundant plasmonic nanogaps, including the interstitial space between the Ag nanostar and Ag nanospheres, as well as internanogaps between neighboring Ag nanospheres, resulting in outstanding SERS performance. It was successfully applied for the quantitative and simultaneous detection of diquat and thiram in fruit juice, achieving detection limits of 5.0 × 10 −8 mol/L and 1 × 10 −8 mol/L, respectively, with recovery rates ranging from 86.6 % to 118.4 %. Notably, under UV light exposure, the composite was able to degrade pesticides efficiently within 80 min. Additionally, its reusability was demonstrated by sustaining strong SERS activity over five continuous detection cycles. These results emphasize the capability of Ag NS/ZIF-8/Ag composites for sensitive pesticide detection via SERS.
研究领域:
分析化学
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纳米技术
·
表面科学
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环境监测
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材料科学
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NANOTECHNOLOGY
·
2019
·Vol. 30
·期 27
·275604
·
IF 3.399
Penghui Li,Wanling Chen,Danni Liu,Hao Huang,Kun Dan,Xiangna Hu,Shaozhou Yu,Paul K Chu,Xue-Feng Yu
We report template growth and dense packing of noble metallic nanoparticles (NPs) on few-layer phosphorene for sensitive surface-enhanced Raman scattering (SERS) detection. Phosphorene obtained by electrochemical exfoliation serves as both the template and reductant in the fabrication of noble metallic NPs which are dispersed on phosphorene without aggregation or pile-up. The BP/Ag/Au and BP/Au/Ag nanocomposites with a nanogap structure exhibit excellent SERS sensitivity and reproducibility with respect to Rhodamine 6G. The BP/Au/Ag nanocomposite is further utilized as the SERS substrate for the detection of two fungicides, thiabendazole and thiram, and detection limits of 10–7 and 10–8 M are achieved. Template growth of Au/Ag nanocomposites is demonstrated to be a green strategy for the fabrication of SERS substrates and has large potential in applications such as pesticide detection.
研究领域:
材料科学
·
纳米技术
·
分析化学
·
环境科学
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Qingyan Han,Hao Zhang,Andrey Kaplan,Shixing Fan,Yazhou Xu,Chengyun Zhang,Wei Gao,Enjie He,Jun Dong
Surface-enhanced Raman scattering (SERS) with ultrahigh sensitivity has garnered significant attention for quantitative analysis and chemically specific detection. However, conventional SERS platforms, typically structured by depositing plasmonic micro/nanoparticles onto rigid substrates, face limitations in further advancement and applications. In this study, we present a novel method to prepare a flexible SERS film substrate composed of graphene overlayer (G) atop a self-assembled array of silver micropopcorns (Ag MPs) and a polymethyl methacrylate (PMMA) membrane. We show that adding an overlayer of graphene improves substantially overall SERS performance. This flexible G/Ag MPs/PMMA film substrate achieves a detection limit as low as 100 picomolar for rhodamine 6G (Rh6G) molecules and identifies mixtures containing Rh6G (10 −11 M) and crystal violet (CV, 10 −6 M) molecules. Furthermore, the flexible SERS structure enables quantitative detection of thiram, achieving a detection limit of 0.005 ppm, well below the allowable limit in food. This study provides a robust SERS platform that advances fundamental research and facilitates the development of practical applications in quantitative analysis, detection, and biological sensing. Combining graphene and Ag MPs on a flexible PMMA substrate offers a versatile and scalable approach to enhancing SERS performance, paving the way for future innovations.
研究领域:
分析化学
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纳米技术
·
材料科学
·
光学传感
·
化学检测
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Nanomaterials
·
2024
·Vol. 14
·期 8
·717
·
IF 4.4
Pengxing Sha,Chushu Zhu,Tianran Wang,Peitao Dong,Xuezhong Wu
In this research, a method was developed for fabricating Au–Au nanorod array substrates through the deposition of large-area Au nanostructures on an Au nanorod array using a galvanic cell reaction. The incorporation of a granular structure enhanced both the number and intensity of surface-enhanced Raman scattering (SERS) hot spots on the substrate, thereby elevating the SERS performance beyond that of substrates composed solely of an Au nanorod. Calculations using the finite difference time domain method confirmed the generation of a strong electromagnetic field around the nanoparticles. Motivated by the electromotive force, Au ions in the chloroauric acid solution were reduced to form nanostructures on the nanorod array. The size and distribution density of these granular nanostructures could be modulated by varying the reaction time and the concentration of chloroauric acid. The resulting Au–Au nanorod array substrate exhibited an active, uniform, and reproducible SERS effect. With 1,2-bis(4-pyridyl)ethylene as the probe molecule, the detection sensitivity of the Au–Au nanorod array substrate was enhanced to 10−11M, improving by five orders of magnitude over the substrate consisting only of an Au nanorod array. For a practical application, this substrate was utilized for the detection of pesticides, including thiram, thiabendazole, carbendazim, and phosmet, within the concentration range of 10−4to 5 × 10−7M. An analytical model combining a random forest and a one-dimensional convolutional neural network, referring to the important variable-one-dimensional convolutional neural network model, was developed for the precise identification of thiram. This approach demonstrated significant potential for biochemical sensing and rapid on-site identification.
研究领域:
分析化学
·
纳米技术
·
机器学习
·
环境科学
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2025
·
IF 3.9
Changjin Zhou,Jiahao Zhang,Hongyi Li,Dong Shao,Junlun Zhu,Wei Wen,Xiuhua Zhang,Zhengfang Tian,Shengfu Wang
As a key technology enabling green and sustainable chemical processes, nanozymes have significantly enhanced their catalytic efficiency and economic viability. Therefore, the development of a higher catalytic activity enzyme mimic to construct a portable and easily sensing strategy for sensitive, rapid, and convenient determination of a specific target is exceedingly elemental for an advanced sensing platform. Herein, we designed and prepared copper-doped palladium metallene (Cu–Pd metallene) with multiple enzyme-like activities for a sensitive colorimetric assay. The relevant kinetic parameters of Cu–Pd metallene with three kinds of enzyme-like activities were evaluated. The Michaelis–Menten constant of laccase mimic and oxidase mimic were calculated to be 0.041 and 0.51 mM, which exhibited high affinity to 2,4-dichlorophenol and 3,3′,5,5′-tetramethylbenzidine in the corresponding system, respectively. Inspired by its high catalytic activity, a simple and portable colorimetric sensing platform based on Cu–Pd metallene was developed to detect and distinguish epinephrine, phenolics, and thiram by UV–vis absorption spectrum and smartphone. Among them, the proposed method can detect epinephrine concentration in the range of 1–15 μg mL–1 by laccase-like activity, and a calculated reliable limit of detection is 0.18 μg mL–1. Owing to the inhibitory effect, the colorimetric sensing platform was used to analyze thiram, which presented a concentration range of 10–500 ng mL–1 and an acceptable detection limit of 4.4 ng mL–1. Considering the advantages of accuracy and ease of use of Cu–Pd metallene-based colorimetric sensing platform, it has great potential for real-time monitoring of relevant targets in poor areas.
研究领域:
纳米技术
·
分析化学
·
生物传感器
·
酶模拟
·
环境监测
·
食品安全
·
即时检测
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