INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2019
·Vol. 20
·期 17
·4111
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IF 4.183
Kangfeng Cai,Huaizhou Gao,Xiaojian Wu,Shuo Zhang,Zhigang Han,Xiaohui Chen,Guoping Zhang,Fanrong Zeng
In this work, the effect of drought on K+uptake in root and its translocation from root to shoot was investigated using six barley genotypes contrasting in drought tolerance. Results showed that drought conditions caused significant changes in K+uptake and translocation in a time- and genotype-specific manner, which consequently resulted in a significant difference in tissue K+contents and drought tolerance levels between the contrasting barley genotypes. The role of K+transporters and channels and plasma membrane (PM) H+-ATPase in barley’s adaptive response to drought stress was further investigated at the transcript level. The expression of genes conferring K+uptake (HvHAK1,HvHAK5,HvKUP1,HvKUP2andHvAKT1) and xylem loading (HvSKOR) in roots were all affected by drought stress in a time- and genotype-specific manner, indicating that the regulation of these K+transporters and channels is critical for root K+uptake and root to shoot K+translocation in barley under drought stress. Furthermore, the barley genotypes showed a strong correlation between H+efflux and K+influx under drought stress, which was further confirmed by the significant up-regulation ofHvHA1andHvHA2. These results suggested an important role of plasma membrane H+-ATPase activity and/or expression in regulating the activity of K+transporters and channels under drought stress. Taken together, it may be concluded that the genotypic difference in drought stress tolerance in barley is conferred by the difference in the ability to regulate K+transporters and channels in root epidermis and stele.Keywords:drought tolerance;ion flux;transmembrane K+transport;plasma membrane H+-ATPase;barley
研究领域:
植物生理学
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分子生物学
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农学
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2019
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IF 8.355
Binrong Li,Huijie Wang,Yiying Lan,Yanhua Cui,Yunlei Zhang,Yonghai Feng,Jianming Pan,Minjia Meng,Chundu Wu
Fe species/biochar fiber composite (FCF) has become one of promising materials for wastewater treatment via heterogeneous photo-Fenton reaction. However, the powdered form always suffers from inefficient recovery and inherent iron loss, limiting their practical application. Herein, we provide an alternative polydopamine (pDA)-modified PVDF bead as excellent photo-Fenton support for immobilizing FCF by phase inversion, which not only limits the loss of iron ions, but also promotes the H 2 O 2 decomposition. Moreover, these beads can float on the wastewater due to their inner structure, favoring the harvest of light. Thanks to these unique characteristics, these beads displayed almost 100% of decolorization in self-designed flowing dyes wastewater systems. Particularly, the self-floating beads structure makes catalysts be easily recovered and maintains long-term high activity, which significantly eliminates the inherent challenge of powdered catalyst (e.g. FCF) based degradation process. This work provides an “easily applicable platform” for large-scale applications of powdered photo-Fenton catalyst in wastewater treatment.
研究领域:
环境科学
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材料科学
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产品关联
DOI
高影响因子
Plant Methods
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2023
·Vol. 19
·期 1
·1-15
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IF 5.1
Liu Hai,Li Yufei,Peng Ting,Xue Shaowu
Background Plant cells usually sustain a stable membrane potential due to influx and/or efflux of charged ions across plasma membrane. With the growth and development of plants, different tissues and cells undergo systemic or local programmed decline. Whether the membrane potential of plasma membrane could report senescence signal of plant tissues and cells is unclear.Results We applied a maneuverable transmembrane potential (TMP) detection method with patch-clamp setup to examine the senescence signal of leaf tissue cells in situ over the whole life cycle in Arabidopsis thaliana. The data showed that the TMPs of plant tissues and cells were varied at different growth stages, and the change of TMP was higher at the vegetative growth stage than at the reproductive stage of plant growth. The distinct change of TMP was detectable between the normal and the senescent tissues and cells in several plant species. Moreover, diverse abiotic stimuli, such as heat stress, hyperpolarized the TMP in a short time, followed by depolarized membrane potential with the senescence occurring. We further examined the TMP of plant chloroplasts, which also indicates the senescence signal in organelles.Conclusion sThis convenient TMP detection method can report the senescence signal of plant tissues and cells, and can also indicate the potential of plant tolerance to environmental stress.
研究领域:
植物学
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细胞生物学
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DOI
PubMed
高影响因子
STAR Protocols
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2022
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IF 0
Qiuxiang Gu,Yao Tian,Junhai Han
Summary In Drosophila , mutations in genes that prevent normal Ca 2+ influx after light stimulation usually cause light-dependent retinal degeneration or neurodegeneration, detectable by defects in eye morphology. Here, we present a protocol based on electron microscopy (EM) to observe the morphological structure of photoreceptor cells in Drosophila . We detail how to fix, dehydrate, embed, and polymerize compound eye samples, followed by sectioning, post-staining, and image acquisition, to assess the eye morphology at the ultrastructural level. For complete details on the use and execution of this protocol, please refer to Gu et al. (2020) .
研究领域:
神经生物学
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细胞生物学
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DOI
JOURNAL OF APPLIED POLYMER SCIENCE
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2024
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IF 2.7
Meirong Wang,Ling Lin,Pingya Luo,Di Pu,Xinmin Zhang,Zheng Li,Hongdan Ao
In this investigation, a new micro-crosslinked anionic polymer resistant to elevated temperature and high salinity, PKDASN, was synthesized using 3-chloropropyltriethoxysilane (KMB703), N, N-Dimethylacrylamide (DMAM), 2-acrylamido-2-methylpropanesulfonic acid (AMPS), N-vinyl caprolactam (NVCL), and sodium (4-vinyl phenyl)methanesulfonate (SVM) employing free radical polymerization in a water-based solution. The structure of PKDASN was analyzed using Fourier-transform infrared spectroscopy, nuclear magnetic resonance spectroscopy, and X-ray photoelectron spectroscopy, while its microstructure was analyzed using scanning electron microscopy. Thermogravimetric analysis showed that the pyrolysis temperature of PKDASN was above 294°C. Adding KMB703 enables the polymer to form a dense and stable spatial network. This intensified polymer network could interact with bentonite via intermolecular force and Coulomb force and form a dual grid structure, which presents impressive stability and control of filtration in saltwater. This is mainly achieved through creating a “silane coupling layer” on the particle surface by KMB703, which facilitates the crosslinking and aggregation of the polymer, thereby reinforcing the polymer's framework. This implies that under high-temperature conditions of 230°C, 3 wt% PKDASN can improve the filtration effectiveness of drilling fluids and exhibit resistance to 15 wt% NaCl contamination. American Petroleum Institute filtration(FL API ) and High temperature and high pressure filtration(FL HTHP ) values were 4.6 and 23.0 mL, respectively. Therefore, this investigation provides a novel approach to utilizing the designated silane agent for the advancement of filtration loss additives in drilling fluids resistant to elevated temperature and high salinity.
研究领域:
材料科学
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石油工程
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DOI
高影响因子
Nano Energy
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2025
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IF 17.1
Shanguo Zhang,Tianyi Jiang,Haoxiu Sun,Ming Li,Depeng Yang,Wenlong Wu,Qi Gu,Aitong Xu,Yu Li,Hongyuan Jiang
Exosomes derived from mesenchymal stem cells (MSCs) exhibit immunomodulatory, tissue repair, anti-inflammatory, and anti-aging properties, making them valuable tools in cell therapy and regenerative medicine. Electrical stimulation shows promise for enhancing exosome production; however, current methods rely on large, expensive power supplies to generate electrical signals. These systems are prone to electrode electrolysis and bubble formation in the buffer solution. In this study, we propose a novel self-powered method based on triboelectric nanogenerators to enhance exosome production from MSCs. This approach utilizes the triboelectric effect to generate an electrical output with an open-circuit voltage of 1800V and a short-circuit current of 88 µA, effectively avoiding harmful electrochemical reactions. By optimizing parameters such as current strength, electrical stimulation duration, and treatment cycles, exosome production is increased by up to 3.2 times. Further investigations reveal that the enhanced exosome production is closely linked to calcium ion influx. Moreover, exosomes produced under electrical stimulation retain their biological activity and significantly promote wound healing. Thus, this self-powered, portable, and efficient electrical stimulation method holds significant potential for advancing exosome production.
研究领域:
细胞生物学
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再生医学
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生物工程
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DOI
高影响因子
Nature Communications
·
2021
·Vol. 12
·期 1
·1-15
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IF 14.919
Xiong Pan,Zhang Fan,Zhang Xiuyun,Liu Yifan,Wu Yunyan,Wang Shijian,Safaei Javad,Sun Bing,Ma Renzhi,Liu Zongwen,Bando Yoshio,Sasaki Takayoshi,Wang Xin,Zhu Junwu,Wang Guoxiu
The regulation of anions and cations at the atomic scale is of great significance in membrane-based separation technologies. Ionic transport regulation techniques could also play a crucial role in developing high-performance alkali metal batteries such as alkali metal-sulfur and alkali metal-selenium batteries, which suffer from the non-uniform transport of alkali metal ions (e.g., Li + or Na + ) and detrimental shuttling effect of polysulfide/polyselenide anions. These drawbacks could cause unfavourable growth of alkali metal depositions at the metal electrode and irreversible consumption of cathode active materials, leading to capacity decay and short cycling life. Herein, we propose the use of a polypropylene separator coated with negatively charged Ti 0.87 O 2 nanosheets with Ti atomic vacancies to tackle these issues. In particular, we demonstrate that the electrostatic interactions between the negatively charged Ti 0.87 O 2 nanosheets and polysulfide/polyselenide anions reduce the shuttling effect. Moreover, the Ti 0.87 O 2 -coated separator regulates the migration of alkali ions ensuring a homogeneous ion flux and the Ti vacancies, acting as sub-nanometric pores, promote fast alkali-ion diffusion.
研究领域:
材料科学
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电化学
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DOI
PubMed
高影响因子
VETERINARY MICROBIOLOGY
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2025
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IF 2.7
Xiaomei Du,Qi Xiao,Li Yang,Yiyi Shan,Yueqing Hu,Wenbin Bao,Shenglong Wu,Zhengchang Wu
Porcine circovirus type 2 (PCV2) is the main pathogen causing postweaning multisystemic wasting syndrome, which leads to enormous losses for porcine industry. However, the regulatory mechanism of PCV2 replication in host cells remains not been clarified. Here, pig DNMT3B was identified as be a host regulator associated with PCV2 infection via RNA-seq analysis. We demonstrated that upregulation of DNMT3B expression can effectively inhibit PCV2 replication in PK15 cells. Besides, TMEM37 acts as a key downstream target of DNMT3B in PCV2-infected PK15 cells. TMEM37 knockdown significantly slowed Ca 2+ influx, and thus inhibited PCV2 replication. Taken together, DNMT3B is required for the PCV2-based infection regulation in host cells. Our findings indicated that DNMT3B inhibits PCV2 replication via targeting TMEM37 to regulate Ca 2+ influx in PK15 cells, which offering a theoretical foundation for the use of this gene as a key biomarker for breeding strategies seeking to improve porcine disease resistance.
研究领域:
兽医学
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分子生物学
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病毒学
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动物遗传学
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产品关联
DOI
PubMed
高影响因子
Journal of Cancer
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2020
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IF 3.565
Ying Song,Chen Li,Yahan Fu,Qiu Xie,Jun Guo,Guangming Li,Huiwen Wu
The integrin receptor protein talin plays vital roles in intracellular chemical and mechanical activities, and it is implicated in the high invasion and poor prognosis of non-small cell lung cancer (NSCLC). To better understand the mechanism underlying the function of talin in NSCLC invasion and metastasis, a few newly designed tension probe based on Förster resonance energy transfer was used for real-time observation of tension changes in A549 cells. High NSCLC cell aggressiveness was found to be accompanied with inward talin and outward glial fibrillary acidic protein (GFAP) tensions, which are closely associated with microfilament (MF) force and intracellular osmotic potential. The increased osmotic pressure resulted from the production of intracellular protein nanoparticles and the related ion influx. Furthermore, integrin activation was found to adjust the talin and GFAP tensions. Disruption of the interaction between talin and MFs blocked the mechanical source of talin, reducing both talin tension and osmotic pressure and thus inhibiting NSCLC cell invasion and migration. Consequently, our study demonstrates that talin is involved in NSCLC invasion and migration via its inward tension and that the integrin pathway is correlated closely with protein-nanoparticle-induced outward osmotic pressure.
研究领域:
癌症生物学
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细胞生物学
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分子生物学
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生物物理学
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产品关联
DOI
PubMed
高影响因子
ACS Nano
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2024
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IF 15.8
Sheng-Hua Liu,Chun-Kui Hu,Jia-Li Lu,Xiaoxiao Lu,Chun-Xin Lu,Juming Yao,Xia-Chao Chen,Lei Jiang
Photoreceptor cells of vertebrates feature ultrastructural membranes interspersed with abundant photosensitive ion pumps to boost signal generation and realize high gain in dim light. In light of this, superstructured optoionic heterojunctions (SSOHs) with cation-selective nanochannels are developed for manipulating photo-driven ion pumping. A template-directed bottom-up strategy is adopted to sequentially assemble graphene oxide (GO) and PEDOT:PSS into heterogeneous membranes with sculptured superstructures, which feature programmable variation in membrane topography and contain a donor–acceptor interface capable of maintaining electron–hole separation upon photoillumination. Such elaborate design endows SSOHs with a much higher magnitude of photo-driven ion flux against a concentration gradient in contrast to conventional optoionic membranes with planar configuration. This can be ascribed to the buildup of an enhanced transmembrane potential owing to the effective separation of photogenerated carriers at the heterojunction interface and the increase of energy input from photoillumination due to a synergistic effect of reflection reduction, broad-angle absorption, and wide-waveband absorption. This work unlocks the significance of membrane topographies in photo-driven transmembrane transportation and proposes such a universal prototype that could be extended to other optoionic membranes to develop high-performance artificial ion pumps for energy conversion and sensing.
研究领域:
材料科学
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纳米技术
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光电技术
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仿生学
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产品关联
DOI
PubMed
高影响因子
IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY
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2023
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IF 3.3
Shiling Hu,Jue Wang,Haoyun Bai,Chaohua Feng,Zhenqi Zhou,Zhuoyin Xue,Wen Zhang,Yongjing Zhang,Nan Wang,Langchong He
Background Mast cells (MCs) are important effector cells in anaphylaxis and anaphylactic disease. 3’,4’,5,7-tetrahydroxyflavone (THF) presents in many medicinal plants and exerts a variety of pharmacological effects. In this study, we evaluated the effect of THF on C48/80-induced anaphylaxis and the mechanisms underlying its effects, including the role of secreted phosphoprotein 1 (SPP1), which has not been reported to IgE-independent MC activation.Results THF inhibited C48/80-induced Ca2+ flow and degranulation via the PLCγ/PKC/IP3 pathway in vitro. RNA-seq showed that THF inhibited the expression of SPP1 and downstream molecules. SPP1 is involved in pseudo-anaphylaxis reactions. Silencing SPP1 affects the phosphorylation of AKT and P38. THF suppressed C48/80-induced paw edema, hypothermia and serum histamine, and chemokines release in vivo.Conclusions Our results validated SPP1 is involved in IgE-independent MC activation anaphylactoid reactions. THF inhibited C48/80-mediated anaphylactoid reactions both in vivo and in vitro, suppressed calcium mobilization and inhibited SPP1-related pathways.
研究领域:
免疫学
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药理学
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细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2021
·
IF 7.086
Jinwen Yuan,Siyan Che,Li Zhang,Xiaomin Li,Junhua Yang,Xiaoming Sun,Zheng Ruan
Food is often exposed to multiple types of contaminants, and the coexistence of contaminants may have antagonistic, additive or synergistic effects. This study investigated the combinatorial toxicity of the three most widespread exogenous contaminants, decabrominated diphenyl ether (BDE-209), bisphenol A (BPA), and acrylamide (ACR) to HepG2 cells. A mathematical model (Chou-Talalay) and high-content analysis (HCA) were used to probe the nature of the contaminants’ interactions and their cytotoxicity mechanisms, respectively. The results highlighted that for the individual pollutants, the cytotoxicity order was BDE-209> BPA > ACR, and varying combinations of contaminants exhibited additive/synergistic effects. In general, combining multiple contaminants significantly increased intracellular reactive oxygen species (ROS), Ca 2+ flux, DNA damage and Caspase-3, and decreased mitochondrial membrane potential (MMP) and nucleus roundness, indicating that the additive or synergistic mechanism of the combined contaminations was disturbance to multiple organelles . This study emphasizes the complexity of human exposure to food contaminants and provides a scientific basis for formulating strict regulatory standards.
研究领域:
毒理学
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环境科学
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细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）