PEST MANAGEMENT SCIENCE
·
2021
·Vol. 78
·期 1
·43-51
·
IF 4.845
Xiaojing Yan,Huizhu Yuan,Yixuan Chen,Xin Shi,Xiaohui Liu,Zhenying Wang,Yue Liu,Daibin Yang
BACKGROUND The fall armyworm (FAW), Spodoptera frugiperda (J. E. Smith), is an invasive pest. Maize is one of its preferred host plants. The traditional application of granules has been effective for the control of FAW. Nevertheless, the challenge is to develop an application method that is less labor-intensive and also efficient. RESULTS Tiny granules in the size range of 0.38–0.55 mm were prepared by the centrifugal granulation method for drone application. The in-swath distribution patterns of granules deposited on sticky tapes were similar to the distribution of aqueous droplets through drone spraying. In the range of 1.5–3.5 m, a relatively higher aircraft height of flight can provide a more uniform distribution pattern of deposited granules, but resulted in less deposit of tiny granules in the whorl of maize plants. Similarly, the deposit of granules in the whorl of maize plants significantly decreased when the flight speed increased from 4 to 6 m s −1 . Granules gathered in the whorl accounted for 50.8–58.7% of total granules deposited in the maize canopy. The field experiment demonstrates granular formulations (containing 0.25% chlorantraniliprole + 0.15% emamectin benzoate, or higher concentrations) can provide better and longer duration of FAW control than the aqueous spray formulation at the whorl stage of maize plants. CONCLUSION Broadcasting of tiny granules by drone can provide better FAW control than liquid spray. The architecture of the maize plant and the downward airstream of the drone create favorable conditions for the deposition of granules in whorls. © 2021 Society of Chemical Industry.
研究领域:
农业昆虫学
·
害虫防治
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DOI
高影响因子
Scientific Reports
·
2020
·Vol. 10
·期 1
·1-10
·
IF 3.998
He Huiqing,Qin Xiangjing,Dong Fangyun,Ye Jingmin,Xu Chunbao,Zhang Hanhui,Liu Zhanmei,Lv Xiaojing,Wu Yuehua,Jiang Xuhong,Cheng Xingan
The invasion of Spodoptera frugiperda has imposed a serious impact on global food security. Matrine is a botanical pesticide with a broad spectrum of insecticidal activity which was recommended for controlling Spodoptera frugiperda . In order to discover effective insecticide for Spodoptera frugiperda , two matrine derivatives modified with carbon disulfide and nitrogen-containing groups were systhesized. And their inhibition activities on Sf9 cell were evaluated. The structural configuration of compounds were characterized by IR, HPLC, MS, NMR and XRD, with yields of 52% and 65%, respectively. The IC 50 of the two newly synthesized compounds on Sf9 cell reduced to 0.648 mmol/L and 1.13 mmol/L, respectively, compared with that of matrine (5.330 mmol/L). In addition, microscopic observation of Sf9 cell treated with the compounds showed that the number of adherent cells decreased, the cells shrunk, vacuolated and apoptotic bodies appeared. The two newly synthesized compounds exhibited better inhibitory effect on Sf9 cell than that of the parent matrine, suggesting that the positive effect of the introduction of 1-pyrrolidinecarbodithioate and diethylcarbamodithioate groups to matrine. The morphological observation of Sf9 cell induced by derivatives indicated that apoptosis induction may be a mechanism that inhibits insect cell proliferation and exerts insecticidal effect.
研究领域:
植物化学
·
昆虫学
·
细胞生物学
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DOI
PubMed
高影响因子
Plants-Basel
·
2024
·Vol. 13
·期 23
·3300
·
IF 4
Jie Liu,Mariam Tallat,Gensong Wang,Guoping Li,Haowen Zhang,Xujin Wu,Hongbo Qiao,Xincheng Zhao,Hongqiang Feng
The fall armyworm,Spodoptera frugiperda(Lepidoptera: Noctuidae) (FAW), is an invasive and destructive polyphagous pest that poses a significant threat to global agricultural production. The FAW mainly damages maize, with a particular preference for V3–V5 (third to fifth leaf collar) plant stages in northern China. How the FAW moth precisely locates maize plants in the V3–V5 stage at night remains unclear. The aims of this study were to evaluate the visual and olfactory cues used by the FAW to identify its host plant, maize, in order to select attractants with better trapping efficacy. Hyperspectral analysis of maize plants at different growth stages using the ASD Fieldspec 4 spectrometer was performed using mimics (moths or maize leaves sealed with transparent plastic sheets) and black cloth-covered plants for single visual and single olfactory attraction experiments. Gas chromatography–mass spectrometry (GC-MS) was used to analyze volatiles emitted from V3–V5 stage maize leaves. Volatile organic chemicals (VOCs) were screened using electroantennography (EAG) and Y-tube. Attractor efficacy was validated using mimics + VOCs. Results showed very little variance in the spectral reflectance curve of the maize at different growth stages. Fifteen VOCs were identified in the V3–V5 stage leaves of three different maize varieties, of whichcis-3-hexenyl acetate and myrcene were found in relatively high concentrations in these maize varieties. The frequency of visits attracted by single visual stimuli was significantly lower than that attracted by single olfactory or olfactory + visual cues. The attractiveness of foliarcis-3-hexenyl acetate increased as its concentration decreased. The combination of mimics +cis-3-hexenyl acetate (1 ng/μL) increased host detection efficiency and stimulated mating behavior. These results indicate that the nocturnal insect FAW primarily uses olfactory cues for host identification, with visual cues serving as a complementary modality. The synergistic effect of olfactory and visual cues increases the efficiency of host recognition. We found thatcis-3-hexenol acetate at a concentration from maize leaves is a reliable olfactory signal for the FAW. When using host plant VOCs as attractants to control adult FAWs, the role of visual cues must be considered.
研究领域:
昆虫学
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植物保护
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产品关联
DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF AGRONOMY
·
2024
·
IF 4.5
Huimin Liao,Zifang Zhou,Yangbeijia Liu,Yuyan Luo,Chenning Zhang,Yuanjiao Feng,Yinghua Shu,Jianwu Wang
It is well-known that increasing crop biodiversity plays an essential role in achieving ecologically based pest management (EBPM); however, the mechanism is poorly understood. The Three Sisters (Maize/Bean/Squash polyculture [MBS]) is a traditional mixed cropping strategy that has been practiced for thousands of years in Mesoamerica; however, mechanistic evidence for its resistance to herbivores is still lacking. In the present study, using both greenhouse and field experiments, we investigated the effects of MBS on the direct and indirect defences of maize ( Zea mays ) against Asian corn borer ( Ostrinia furnacalis ) and fall armyworm ( Spodoptera frugiperda ) caterpillars. MBS significantly improved the direct chemical defences of maize, especially against O. furnacalis , by increasing the content of primary metabolites, secondary metabolites (Benzoxazinoids), and phytohormones ((±)-Jasmonic acid-isoleucine, Jasmonic acid) in maize leaves, thus significantly reducing their survival and relative growth rates. MBS significantly improved the indirect chemical defences of maize by varying the species and content of volatile organic compounds (such as a mixture of nonanal, 1-dodecanol and 2-butyl-1-octanol), seemingly resulting in the avoidance of MBS plants by both herbivores in the Y-tube olfactometer tests. Field experiments further demonstrated that MBS significantly reduced damage to maize leaves by herbivores, enhanced maize yields, and increased the abundance of natural enemies. Therefore, we concluded that MBS improves the resistance of maize to major pests, and can be used as an effective and eco-friendly crop protection strategy in agroecosystems.
研究领域:
农业生态学
·
植物保护学
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产品关联
DOI
高影响因子
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
·
2023
·Vol. 120
·期 37
·e2308685120
·
IF 11.1
Yu Shi,Qingqing Liu,Wenjie Lu,Jing Yuan,Yihua Yang,John Oakeshott,Yidong Wu
Here, we provide mechanistic support for the involvement of the CYP9A subfamily of cytochrome P450 monooxygenases in the detoxification of host plant defense compounds and chemical insecticides in Spodoptera exigua and Spodoptera frugiperda. Our comparative genomics shows that a large cluster of CYP9A genes occurs in the two species but with significant differences in its contents, including several species-specific duplicates and substantial sequence divergence, both between orthologs and between duplicates. Bioassays of CRISPR-Cas9 knockouts of the clusters show that, collectively, the CYP9As can detoxify two furanocoumarin plant defense compounds (imperatorin and xanthotoxin) and insecticides representing three different chemotypes (pyrethroids, avermectins, and oxadiazines). However, in vitro metabolic assays of heterologously expressed products of individual genes show several differences between the species in the particular CYP9As with activities against these compounds. We also find that the clusters show tight genetic linkage with high levels of pyrethroid resistance in field strains of the two species. We propose that their divergent amplifications of the CYP9A subfamily have not only contributed to the development of the broad host ranges of these species over long evolutionary timeframes but also supplied them with diverse genetic options for evolving resistance to chemical insecticides in the very recent past.
研究领域:
昆虫学
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分子生物学
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基因组学
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毒理学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF INSECT PHYSIOLOGY
·
2025
·
IF 2.3
Xu Wu,Wen-Mei Li,Qiang Xu,Xiao-Ping Yi,Da-He Zhang,Ming-Zhe He,Yu-Hong Ren
RNA interference (RNAi) has shown excellent potential for pest management worldwide. However, the application of RNAi against insects can be inefficient and unreliable. Finding a better delivery system is a crucial factor for enhancing RNAi efficacy. Here, we present a novel and effective approach of conjugating double-stranded RNA (dsRNA) with a cell-penetrating disulfide polymer (CPD) to improve dsRNA stability and RNAi efficiency. The fall armyworm (FAW), Spodoptera frugiperda , is a globally agricultural insect. Chitin synthase B ( CHSB ) and methoprene-tolerant ( Met ) genes, which are essential for the development and growth of FAW, were selected as target genes. The CPD was synthesized using a two-step method for dsRNA delivery. The synthesized CPD/dsRNA complex protected dsRNA from nuclease degradation. The biological application of CPD in Sf9 cells demonstrated low cytotoxicity and high cell viability. Moreover, the CPD-loaded dsRNA entered the cells within 6 h. Bioassays of FAW showed that the relative expression levels of the CHSB and Met genes were reduced. Specifically, at 72 h, the relative expression levels of the CHSB and Met genes were 48.14 % and 37.60 % of those in the control group, respectively. The weight and body length of the larvae decreased significantly, and the mortality rate of CPD/ds CHSB reached 30 %. This CPD was demonstrated for the first time to have excellent delivery performance in insects and is expected to become a new and effective tool for pest control, representing a significant advancement in pest management.
研究领域:
昆虫学
·
分子生物学
·
害虫治理
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生物技术
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RNA干扰技术
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产品关联
DOI
PubMed
高影响因子
Insect Science
·
2024
·
IF 2.9
Wen-Xuan Qi,Feng Liu,Fang-Fang Liu,Hai-Yan Ren,Bang-Xian Zhang,Xiao-Qiang Yu,Xiang-Jun Rao
Antimicrobial peptides (AMPs) are critical components of innate immunity in diverse organisms, including plants, vertebrates, and insects. This study identified and characterized a novel Lepidoptera-specific AMP, named lepidoptin, from the invasive pest Spodoptera frugiperda (Lepidoptera: Noctuidae). Lepidoptin is a 116-amino acid protein containing a signal peptide and a novel β -sandwich domain that is distinct from previously reported AMPs. Temporal and spatial expression analyses revealed a significant upregulation of the lepidoptin gene in vivo and in cultured SF9 cells in response to pathogens. Molecular docking analysis identified a specific binding cavity. Enzyme-linked immunosorbent assay and binding assays confirmed that lepidoptin can bind to pathogen-associated molecular patterns, bacteria, and fungi. Recombinant lepidoptin exhibited potent antibacterial activity by inducing bacterial agglutination, inhibiting bacterial growth, increasing bacterial membrane permeability, and preventing biofilm formation. Lepidoptin also showed antifungal activity against the entomopathogenic fungus Beauveria bassiana by inhibiting spore germination, increasing fungal cell permeability, and increasing reactive oxygen species. Injection of recombinant lepidoptin into S. frugiperda larvae increased survival after B. bassiana infection, whereas knockdown of lepidoptin by RNA interference decreased larval survival. In addition, lepidoptin showed antimicrobial activity against the plant pathogen Fusarium graminearum by inhibiting spore germination and alleviating disease symptoms in wheat seedlings and cherry tomatoes. This study demonstrates the remarkable dual functionality of lepidoptin in enhancing S. frugiperda immunity and controlling plant pathogens, making it a promising candidate for biocontrol strategies in both pest management and plant disease prevention.
研究领域:
昆虫学
·
微生物学
·
分子生物学
·
植物病理学
·
害虫管理
·
生物防治
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）