JOURNAL OF PLANT RESEARCH
·
2023
·Vol. 136
·期 5
·631-641
·
IF 2.8
Wang Hongfei,Wang Xiaofeng,Li Yongjia,Gao Rui,Narsing Rao Manik Prabhu,Song Jieqiong,Li Qiuli
Environmental conditions during seed development and maturation can affect seed traits and germination behavior, yet systematic research on the effects of seed maturation time on seed traits, germination behavior and seedling emergence of cleistogamy plants is lacking. Here, we determined the difference in phenotypic characteristics of CH and CL (namely CL1, CL2 and CL3 based on maturation time, respectively) fruits/seeds that were collected from Viola prionantha Bunge, a cleistogamous perennial plant, and evaluated the effects of various environmental factors on seed germination and seedling emergence. The fruit mass, width, seed number per fruit and mean seed mass of CL1 and CL3 were greater than that of CH and CL2, while seed setting of CH was lower than that of CL1, CL2 and CL3. Germination of CH, CL1, CL2, and CL3 seeds was < 10% in the dark at 15/5 and 20/10 ℃, whereas germination (0%–99.2%) of CH, CL1, CL2, and CL3 seeds changed significantly under light conditions. In contrast, more than 71% (from 71.7 to 94.2%) germination of both CH, CL1, CL2 and CL3 seeds occurred under both light/dark conditions and continuous darkness at 30/20 ℃. Germination of CH, CL1, CL2 and CL3 seeds was sensitive to osmotic potential, but CL1 seeds were more resistant to osmotic stress, compared with CH, CL2 and CL3. Seedling emergence of CH seeds was more than 67% (from 67.8 to 73.3%) at a burial depth of 0 cm–2 cm, while all types of CL seeds were below 15% at a burial depth of 2 cm. Information gathered from this study indicates that CH and CL seeds of V. prionantha were different in fruit size, seed mass, thermoperiod and photoperiod sensitivity, osmotic potential tolerance and seedling emergence, especially, maturation time significantly affect phenotypic characteristics and germination behavior of CL seeds matured at different periods. These results indicate that V. prionantha adapts to unpredictable environmental conditions by developing a variety of adaptation strategies, and ensures the survival and reproduction of the populations.
研究领域:
植物学
·
生态学
✓ 已验证
DOI
高影响因子
JOURNAL OF PLANT PHYSIOLOGY
·
2022
·
IF 3.686
Shuangxi Zhang,Huachun Sheng,Yue Ma,Yanping Wei,Dan Liu,Yanhua Dou,Huiying Cui,Boyou Liang,Johannes Liesche,Jisheng Li,Shaolin Chen
Cell wall biogenesis is required for the production of seeds of higher plants. However, little is known about regulatory mechanisms underlying cell wall biogenesis during seed formation. Here we show a role for the phosphorylation of Arabidopsis cellulose synthase 1 (AtCESA1) in modulating pectin synthesis and methylesterification in seed coat mucilage. A phosphor-null mutant of AtCESA1 on T166 ( AtCESA1 T166A ) was constructed and introduced into a null mutant of AtCESA1 ( Atcesa1-1 ). The resulting transgenic lines showed a slight but significant decrease in cellulose contents in mature seeds. Defects in cellulosic ray architecture along with reduced levels of non-adherent and adherent mucilage were observed on the seeds of the AtCESA1 T166A mutant. Reduced mucilage pectin synthesis was also reflected by a decrease in the level of uronic acid. Meanwhile, an increase in the degree of pectin methylesterification was also observed in the seed coat mucilage of AtCESA1 T166A mutant. Change in seed development was further reflected by a delayed germination and about 50% increase in the accumulation of proanthocyanidins, which is known to bind pectin and inhibit seed germination as revealed by previous studies. Taken together, the results suggest a role of AtCESA1 phosphorylation on T166 in modulating mucilage pectin synthesis and methylesterification as well as cellulose synthesis with a role in seed development.
研究领域:
植物生物学
·
分子遗传学
·
细胞壁生物合成
✓ 已验证
DOI
PubMed
高影响因子
SEED SCIENCE RESEARCH
·
2021
·Vol. 31
·期 2
·136-148
·
IF 2.25
Chengshuai Li,Lijing Zhang,Decao Niu,Shuzhen Nan,Xiumei Miao,Xiaowei Hu,Hua Fu
Flavonoids are a group of phenolic secondary metabolites in plants that have important physiological, ecological and economic value. In this study, using the desert plant Artemisia sphaerocephala Krasch. as the sample material, the content and components of the total flavonoids in its seeds at seven different developmental stages were determined. In addition, the genes involved in flavonoid metabolism were identified by full-length transcriptome sequencing (third-generation sequencing technology based on PacBio RS II). Their expression levels were analysed by RNA-seq short reading sequencing, to reveal the patterns and regulation mechanisms of flavonoid accumulation during seed development. The key results were as follows: the content of total flavonoids in mature seeds was 15.05 mg g−1, including five subclasses: flavonols, chalcones, flavones, flavanones and proanthocyanidins, among which flavonols accounted for 45.78%. The period of rapid accumulation of flavonoids was 40–70 d following anthesis. The high expression of phenylalanine ammonia-lyase (PAL), 4-coumarate-CoA ligase (4CL) and UDP-glucose:flavonoids 3-o-glucosyltransferase (UF3GT) promoted the accumulation of total flavonoids, while the high expression of flavonoids 3′-hydroxylase (F3′H) and flavonols synthase (FLS) made flavanols the main component. Transcription factors such as the MYB-bHLH-WDR (MBW) complex and Selenium-binding protein (SBP) directly regulated the structural genes of flavonoid metabolism, while C2H2-type zinc finger (C2H2), Zinc-finger transcription factor (GATA), Dehydration-responsive element binding (DREB), Global Transcription factor Group E protein (GTE), Trihelix DNA-binding factors (Trihelix) and Phytochrome-interacting factor (PIF) indirectly promoted the synthesis of flavonoids through hormones such as brassinoidsteroids (BRs) and abscisic acid (ABA). These results provided valuable resources for the application of related genes in genetics and breeding.
研究领域:
植物生理学
·
分子生物学
·
生物化学
·
基因组学
✓ 已验证
产品关联
DOI
高影响因子
Advanced Science
·
2024
·Vol. 11
·期 42
·2407543
·
IF 14.3
Simin Bi,Jianpeng Ao,Ting Jiang,Xianmiao Zhu,Yimin Zhu,Weibing Yang,Binglian Zheng,Minbiao Ji
Water plays a vital role in the life cycle of plants, participating in various critical biochemical reactions during both non-photosynthetic and photosynthetic processes. Direct visualization of the metabolic activities of water in plants with high spatiotemporal resolution is essential to reveal the functional utilization of water. Here, stimulated Raman scattering (SRS) microscopy is applied to monitor the metabolic processes of deuterated water (D 2 O) in model plant Arabidopsis thaliana (A. thaliana) . The work shows that in plants uptaking D 2 O/water solution, proton-transfer from water to organic metabolites results in the formation of C-D bonds in newly synthesized biomolecules (lipid, protein, and polysaccharides, etc.) that allow high-resolution detection with SRS. Reversible metabolic pathways of oil-starch conversion between seed germination and seed development processes are verified. Spatial heterogeneity of metabolic activities along the vertical axis of plants (root, stem, and tip meristem), as well as the radial distributions of secondary growth on the horizontal cross-sections are quantified. Furthermore, metabolic flow of protons from plants to animals is visualized in aphids feeding on A. thaliana . Collectively, SRS microscopy has potential to trace a broad range of matter flows in plants, such as carbon storage and nutrition metabolism.
研究领域:
植物生理学
·
分子生物学
·
成像技术
·
代谢组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PLANT GROWTH REGULATION
·
2022
·Vol. 42
·期 4
·2162-2175
·
IF 4.64
Chen Chen,Chen Hong,Han Chao,Liu Zemao,Yu Fangyuan,Wu Qikui
Due to the increasing demand for biofuel feedstock production, Styrax tonkinensis , as a woody oilseed tree species, has received much more attention. However, the effects of various plant growth regulators on its seed development haven’t been explored deeply. Here, we applied 24-epibrassinolide (EBL) and methyl jasmonate (MJ) on the whole trees, and the aim of our study was to explore their effects on seed development of S. tonkinensis as well as seed chemical compositions, especially seed lipid metabolism. The results showed that EBL and MJ promoted the seed development, which was reflected in the increase of fresh and dry weight, as well as transverse and longitudinal diameter. The concentration of crude fat (CF) exhibited an ‘increase–decrease-increase’ trend, and the peak appeared at 70 days after flowering. EBL (in concentration of 5 μM, EBL5) and MJ (in concentration of 200 μM, MJ200) had the most significant effect on the accumulation of CF and the CF content in single seed. In addition, the activities of enzymes related to fatty acid (FA) synthesis were also higher under the two treatments. 12 FAs were detected using gas chromatography-mass spectrometry, among which palmitic acid, oleic acid, and linoleic acid were the main components. EBL5, EBL10, and MJ200 favored the accumulation of soluble sugar, especially in the middle and late stages. The starch concentration in seeds was lower, but it significantly increased after the application of EBL5. Furthermore, our results demonstrated that EBL and MJ enhanced SOD and POD activity while decreased MDA content. We highlighted that EBL and MJ promoted seed development of S. tonkinensis , affected seed chemical compositions and contributed to the accumulation of CF and FA.
研究领域:
植物科学
·
生物化学
·
农业
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）