ACTA PHYSIOLOGIAE PLANTARUM
·
2010
·Vol. 33
·期 4
·1271-1276
·
IF 1.232
Guan Yajing,Hu Jin,Li Yongping,Ma Wenguang,Zheng Yunye
Counterfeit seeds cause a large loss in seed industry and crop production. Present study was undertaken to find a new anti-counterfeiting technology by labeling seeds with a fluorescent compound, safranine T (ST). Response surface analysis (RSA) experiment with two factors (ST concentration and seed soaking time) of 14 combinations was performed to investigate the ST effects on seed vigor and fluorescence showing in seedlings of two tobacco varieties, MS Yunyan 85 and Honghua Dajinyuan. After soaking in ST solutions, tobacco seeds were germinated for 16 days, then the germination index (GI), vigor index (VI), length of roots and shoots were determined. The optimum combinations of ST concentration and soaking time on GI, VI and length of shoots and roots were 0.57–0.69 mg/ml and 6.77–9.34 h for MS Yunyan 85 and 0.51–0.80 mg/ml and 5.81–7.39 h for Honghua Dajinyuan, respectively. Meanwhile, tobacco seedlings, treated with ST solutions from 0.3 to 1.5 mg/ml showed obviously fluorescence under illumination of green light. It suggests that soaking tobacco seeds in safranine T solution of suitable concentration can be used as a labeling technique for anti-counterfeiting in tobacco seeds.
研究领域:
种子科学
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农业生物技术
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植物生理学
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分析化学
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作物生产
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DOI
高影响因子
MATERIALS LETTERS
·
2020
·
IF 3.204
Jun Zhu,Gongguo Zhang,Weiwei Chang,Xiaoqian Luan,Fengli Qu,Yiqun Zheng
We report a facile synthesis of silver nanorods/nanowires with the diameter in the range of sub-20 nm via seeded growth. The key to the success of this work relies on the use of tiny Au seeds and manipulation over reaction kinetics. In particular, their diameter could be controlled to as small as 12 nm using 5-nm spherical Au seeds. They can be further converted to trimetallic (Au@Ag-Pd) hollow derivatives with tunable hollow degree and elemental compositions by galvanic replacement reaction.
研究领域:
材料科学
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纳米技术
·
化学合成
✓ 已验证
产品关联
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2020
·
IF 6.551
Xiaowen Wang,Ajay Jain,Xu Huang,Xiaoxia Lan,Li Xu,Gengmao Zhao,Xin Cong,Zhantian Zhang,Xiaorong Fan,Feng Hu
The uptake and accumulation of polycyclic aromatic hydrocarbons (PAHs) in crops have gained much attention due to their toxicity to humans. Nitrogen (N) is an essential element for plant growth and has also been implicated in the acquisition and acropetal translocation of PAHs. OsNRT2 . 3b encodes a nitrate (NO 3 − ) transporter that is involved in the acquisition and mobilization of N in rice. Here, we investigated whether overexpression of OsNRT2 . 3b would exert any mitigating influence on the uptake and translocation of phenanthrene (Phe, a model PAH) in transgenic rice ( Oryza sativa ). The wild-type seedlings exhibited a reduction in plant height, primary root length, and shoot biomass when grown hydroponically in a medium supplemented with Phe. Acquisition of Phe by the roots and its subsequent translocation to shoots increased concomitantly with an increase in Phe concentration in the medium and duration of the treatment. OsNRT2 . 3b -overexpressing lines (Ox-6 and Ox-8) were generated independently. Compared with the wild-type, the concentration of Phe in Ox-6 and Ox-8 were significantly lower in the roots (47%–54%) and shoots (22%–31%) grown hydroponically with Phe (1 mg/L). Further, the wild-type and Ox lines were grown to maturity in a pot soil under Phe conditions and the concentrations of Phe and total N were assayed in the culms and flag leaves. Compared with the wild-type, in Ox lines the concentration of total N significantly increased in the culms (288%–366%) and flag leaves (12%–25%), while that of Phe significantly reduced in the culms (25%–28%) and flag leaves (18%–21%). The results revealed an antagonistic correlation between the concentration of total N and Phe. The concentration of Phe was also significantly lower (29%–38%) in the seeds of Ox lines than the wild-type. The study highlighted the efficacy of overexpressing OsNRT2 . 3b in mitigating the Phe toxicity by attenuating its acquisition, mobilization, and allocation to the seeds.
研究领域:
植物学
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环境科学
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遗传学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF STORED PRODUCTS RESEARCH
·
2025
·
IF 2.8
Shengjun Wu,Ying Zhu,Chengxuan Li
Peanut seeds are highly susceptible to fungal contamination and insect infestation during ambient storage, leading to quality degradation, reduced germination, and hazardous aflatoxin B1 (AFB1) accumulation. This study evaluated the preservation efficacy of chitosan/cinnamaldehyde (CS/CA) composite coatings on peanut seeds during 12 months of storage. The synergistic action of CS (antimicrobial film-forming polymer) and CA (antifungal/antioxidant essential oil component) provided dual protection against biotic and oxidative damage. Compared to the untreated control, seeds coated with CS/0.5 % CA exhibited significantly reduced fungal proliferation (final count 2.0 log CFU/g versus 5.9 log CFU/g in control), diminished insect damage (1.9 % versus 4.6 %), and maintained enhanced germination capacity (82 % versus 61 %) over a 12-month storage period. Chitosan/cinnamaldehyde (CS/CA) composite coatings preserved peanut seeds for 12 months in this investigation. Antibiotic film-forming polymer CS and antifungal/antioxidant essential oil CA reduced biotic and oxidative damage. The controls had more fungal and insect damage than the CS/CA-treated seeds. CA formulations at 0.5 % and 1.0 % reduced Aspergillus flavus growth and AFB1 accumulation, resulting in final values of 7.0 and 6.0 μg/kg, much below the global regulatory limit of 20 μg/kg. CA also improved seed vigor measures, such as germination power and normal seedling rate The 1.0 % CA group had similar antifungal efficacy but reduced germination. The results indicate that the 0.5 % CS/CA coating is especially efficacious in extending seed viability, assuring safety, and preserving germination performance under ambient storage circumstances.
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DOI
高影响因子
RSC Advances
·
2022
·Vol. 12
·期 33
·21223-21234
·
IF 4.036
Inam Ullah Khan,Abdul Haleem,Assad Ullah Khan
Currently, the energy crisis is a hot topic for researchers because we are facing serious problems due to overpopulation and natural energy sources are vanishing day-by-day. To overcome the energy crisis, biofuel production from non-edible plant seeds is the best solution for the present era. In the present study, we select the non-edible seeds of Acacia farnesiana for biofuel production from different areas of Pakistan with better oil production results. Different kinds of analytical method, like the American Standard for Testing and Materials and techniques like Fourier transform infra-red spectroscopy, nuclear magnetic resonance spectroscopy, gas chromatography, and inductively coupled plasma optical emission spectrometry, were used to evaluate the chemical compositions. The maximum oil extraction rate (23%) was produced by petroleum ether. Potassium hydroxide exhibited the best conversion result of 96% fatty acid methyl ester. The transesterification method was used for the preparation of fatty acid methyl ester (96%) using potassium hydroxide and methanol. The viscosity and density of Acacia farnesiana seed oil biodiesel was comparable to American Standard for Testing Material biodiesel standards. By using gas chromatography-mass spectrometry, five fatty acids were detected comprising palmitic acid (6.85%), stearic acid (2.36%), oleic acid (12.13%), linoleic acid (46.85%), and α-linolenic acid (1.23%). This study concludes that Acacia farnesiana seed oil biodiesel could be an intriguing raw material for yielding Acacia farnesiana seed oil methyl ester as an alternative fuel source.
研究领域:
能源植物学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Fei Qu,Zhuo Wang,Cong Li,Dafeng Jiang,Xian-en Zhao
Herein, a novel peptidyl probe is first synthesized by the amide reaction between glutathione-capped gold nanoclusters (GSH-AuNCs) and positive charged arginine-rich peptide (Arg 9 ), and it is called Arg 9 -GSH-AuNCs. Polyuridylic acid (polyU), as a polyanion , is firstly used to induce the aggregation-induced emission enhancement (AEE) of positive charged Arg 9 -GSH-AuNCs through electrostatic attraction . The peptide bond on the carboxyl terminal of the arginine residues can be recognized and cleaved by trypsin. Hence, polyU is hard to induce the aggregation of peptidyl probe again, and the AEE from GSH-AuNCs becomes weak. It is an ideal platform to detect trypsin and screen trypsin inhibitors . The fluorescent peptidyl probe exhibits excellent sensitivity for trypsin. A good linear range of trypsin is obtained from 1 to 50 ng·mL −1 with the limit of detection (LOD) 0.31 ng·mL −1 , which is lower than those of most reported works. Furthermore, the inhibitory effects of the extracts from foods (i.e., soybean seed flour, black soybean seed flour, mung bean seed flour, and azuki bean seed flour) on the trypsin activity have been studied. The half maximal inhibitory concentration (IC 50 ) values of extracts from soybean seed flour, black soybean seed flour, mung bean seed flour, and azuki bean seed flour are 4.5, 9.3, 11.6, 20.9 μg·mL −1 , respectively. The screening results show that these extracts from foods all have inhibitory effects on trypsin. Among them, the extract from soybean seed flour exhibits significant inhibitory effect.
研究领域:
分析化学
·
生物化学
·
食品科学
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产品关联
DOI
高影响因子
Molecular Plant
·
2024
·
IF 17.1
Xiaoming Li,Zhonghui Chen,Haiyang Li,Lin Yue,Cuirong Tan,Hongjie Liu,Yilong Hu,Yuhua Yang,Xiani Yao,Lingping Kong,Xiang Huang,Bin Yu,Chunyu Zhang,Yuefeng Guan,Baohui Liu,Fanjiang Kong,Xingliang Hou
Soybean is a photoperiod-sensitive short-day crop whose reproductive period and yield are markedly affected by day-length changes. Seed weight is one of the key traits determining the soybean yield; however, the prominent genes that control the final seed weight of soybean and the mechanisms underlying the photoperiod’s effect on this trait remain poorly understood. In this study, we identify SW19 as a major locus controlling soybean seed weight by QTL mapping and determine Dt1 , an orthologous gene of Arabidopsis TFL1 that is known to govern the soybean growth habit, as the causal gene of the SW19 locus. We showed that Dt1 is highly expressed in developing seeds and regulates photoperiod-dependent seed weight in soybean. Further analyses revealed that the Dt1 protein physically interacts with the sucrose transporter GmSWEET10a to negatively regulate the import of sucrose from seed coat to the embryo, thus modulating seed weight under long days. However, Dt1 does not function in seed development under short days due to its very low expression. Importantly, we discovered a novel natural allelic variant of Dt1 (H4 haplotype) that decouples its pleiotropic effects on seed size and growth habit; i.e., this variant remains functional in seed development but fails to regulate the stem growth habit of soybean. Collectively, our findings provide new insights into how soybean seed development responds to photoperiod at different latitudes, offering an ideal genetic component for improving soybean’s yield by manipulating its seed weight and growth habit.
研究领域:
植物学
·
遗传学
·
作物科学
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DOI
PubMed
高影响因子
Current Research in Food Science
·
2024
·
IF 6.2
Habtamu Kefale,Rong Zhou,Zishu Luo,Senouwa Segla Koffi Dossou,Muez Berhe,Lei Wang,Ahmed A. Abbas,Yanxin Zhang,Ting Zhou,Jun You,Linhai Wang
Black oilseed crops are rich in diverse phenolic compounds and have excellent antioxidant activities, as reported in traditional Chinese medicine. Testa (seed coat) and peeled seeds (cotyledon, embryo, and other structures) are the seed's crucial components, contributing to the variation in phytonutrient, phenol content, bioactive component, and protective and pharmacological effects. However, comprehensive and comparative information on total phenol, flavonoid, antioxidant, and metabolic profiles in black seed testa and peeled sesame, soybean, peanut, and rapeseed seeds is rare. Here, we investigated the metabolic profiles, phenolic contents, and antioxidant activities of four black oilseed crop testas and peeled seeds. This study revealed that testa has higher total phenol, flavonoid, and antioxidant activities than peeled seeds. A total of 1847 metabolites were identified across all samples and categorized into 17 major classes: flavonoids (20.02%), phenolic acids (15.15%), lipids (11.47%), amino acids and derivatives (9.36%), alkaloids (7.47%), organic acids (5.79%), terpenoids (5.68%), lignans (5.57%), saccharides (4.27%), and nucleotides and derivatives (4.17%) among the top ten. Primary class metabolites such as amino acids, saccharides, and vitamins were higher in the peeled seeds than in the testa, signifying the role of energy reservoirs and nutritive potential. However, flavonoids, phenolic acids, coumarins, chromones, lignans, terpenoids, tannins, organic acids, and lipids were abundant in the testa. Interestingly, the diversity and content of secondary metabolites were more abundant in the testa than in the peeled seeds of each crop, explaining their potential for phenol content, bioactivity, antioxidant activity, and pharmacological potential. The bioactivity of peeled seeds and testas may be associated with the phytochemical composition and content of flavonoids, phenolic acids, terpenoids, alkaloids, lipids, terpenoids, lignans, amino acids, and saccharides. Therefore, according to our results, peeled seeds offer higher nutritional value, and the testa has medicinal and protective properties. This study provides insights into the variations in phytochemical composition, phenolic content, and antioxidant activity of testa and peeled black sesame, soybean, peanut, and rapeseed seeds for further application of oilseeds in food products and to maximize nutritional benefits.
研究领域:
农业
·
食品科学
·
药理学
·
代谢组学
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2014
·
IF 3.208
Yixin Tian,Zhan Li,Fei He,Yajing Guan,Shuijin Zhu,Jin Hu
Combining with seed coating method, rhodamine B (RB), a kind of fluorescent compounds had been used in seed labeling as an anti-counterfeiting technology. Effects of broad bean seed coating with RB on physiological parameters and fluorescence performance in broad bean seedlings were performed. The prolonged fluorescence in vascular bundles of seedlings treated with RB could be used as a marker of seed anti-counterfeiting. The experiment showed that RB had no negative effect on seed germination, seedling growth, seedling protective enzyme activities, and the contents of malondialdehyde (MDA) and chlorophyll, after coating seeds with ratio 1 kg RB to 10–30 kg seeds. In addition, the RB adsorption by soil was accorded with the isothermal adsorption equations of Langmuir, which exhibited the linear correlation as 0.9959, the maximum adsorption as 1.716 mg/g, and the adsorption equilibrium constant as 0.042 L/mg. The soil adsorption of RB could be effectively degraded by UV/Fenton. Present research suggested that coating broad bean seeds with appropriate concentration RB were safe to seed germination and this technology was suitable for anti-counterfeiting application.
研究领域:
农业
·
种子科学
·
生物技术
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Plant Science
·
2018
·
IF 3.677
Li Zhan,Gao Yue,Zhang Yuchan,Lin Cheng,Gong Dongting,Guan Yajing,Hu Jin
Seed germination is a complex process controlled by various mechanisms. To examine the potential contribution of reactive oxygen species (ROS) and gibberellin acid (GA) in regulating seed germination, diphenylene iodonium chloride (DPI) and uniconazole (Uni), as hydrogen peroxide (H2O2) and GA synthesis inhibitor, respectively, were exogenously applied on tobacco seeds using the seed priming method. Seed priming with DPI or Uni decreased germination percentage as compared with priming with H2O, especially the DPI + Uni combination. H2O2 and GA completely reversed the inhibition caused by DPI or Uni. The germination percentages with H2O2 + Uni and GA + DPI combinations kept the same level as with H2O. Meanwhile, GA or H2O2 increased GA content and deceased ABA content through corresponding gene expressions involving homeostasis and signal transduction. In addition, the activation of storage reserve mobilization and the enhancement of soluble sugar content and isocitrate lyase (ICL) activity were also induced by GA or H2O2. These results strongly suggested that H2O2 and GA were essential for tobacco seed germination and by downregulating the ABA/GA ratio and inducing reserve composition mobilization mutually promoted seed germination. Meanwhile, ICL activity was jointly enhanced by a lower ABA/GA ratio and a higher ROS concentration.
研究领域:
植物生理学
·
分子生物学
·
植物学
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产品关联
DOI
PubMed
高影响因子
PLANT MOLECULAR BIOLOGY
·
2025
·Vol. 115
·期 2
·1-13
·
IF 3.8
Liu Yitong,Liu Shuangrong,Jing Yanjun,Li Jialong,Lin Rongcheng
Seed dormancy enables plants to delay germination until conditions are favorable for the survival of the next generation. Seed dormancy and germination are controlled by a combination of external and internal signals, in which light and ethylene act as critical regulators. However, how light and ethylene are interlinked to control these two processes remains to be investigated. Here, we show that ethylene and its precursor, 1-aminocyclopropane-1-carboxylic acid (ACC), promote seed germination under light. Light facilitates the conversion of ACC to ethylene, in which phytochrome B (phyB) and FAR-RED ELONGATED HYPOCOTYL3 (FHY3) are functionally required. ACC oxidases (ACOs) catalyze the conversion of ACC to ethylene, among which ACO1 is specifically and predominantly expressed in imbibed seeds. Ethylene induces FHY3 protein accumulation in imbibed seeds, whereby FHY3 directly binds to ACO1 promoter and specifically mediates light-promoted ACO1 expression. Light promotes ACO1 protein accumulation. Overexpression of ACO1 significantly promotes seed germination, and almost completely restores the dormant defect of fhy3 loss-of-function mutants. In summary, this study reveals an ethylene-responsive regulatory cascade of phyB-FHY3-ACO1 that integrates external light input with internal factors to regulate seed dormancy and germination.
研究领域:
植物生物学
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分子遗传学
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发育生物学
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产品关联
DOI
PubMed
高影响因子
PLoS One
·
2021
·Vol. 16
·期 9
·e0257236
·
IF 3.24
Zong He Zhu,Abdul Sami,Qing Qing Xu,Ling Ling Wu,Wen Yin Zheng,Zhi Peng Chen,Xue Zhi Jin,Hong Zhang,Yong Li,Yan Yu,Ke Jin Zhou
The present study was performed to evaluate the effects of seed priming. This was done by soaking the seeds of two rapeseed cultivars, namely, ZY15 (tolerant to low temperature and drought) and HY49 (sensitive to low temperature and drought), for 12 h in varying solutions: distilled water, 138 mg/L salicylic acid (SA), 300 mg/L gibberellic acid (GA), 89.4 mg/L sodium nitroprusside (SNP), 3000 mg/L calcium chloride (CaCl 2 ), and 30 mg/L abscisic acid (ABA). Primed and non-primed seeds were left to germinate at 15°C and -0.15 MPa (T 15 W 15 ) and at 25°C and 0 MPa (T 25 W 0 ), respectively. The results showed that SA, GA, SNP, CaCl 2 , and ABA significantly improved the germination potential (GP), germination rate (GR), germination index (GI), stem fresh weight (SFW), stem dry weight (SDW), root length (RL), stem length (SL), and seed vigor index (SVI) under T 15 W 15 . For ZY15 seeds under T 25 W 0 , GA, SNP, CaCl 2 , and ABA priming reduced the average germination time (96% after 5 days) compared to that of the control (88% after 5 days). For ZY15 seeds under T 15 W 15 , SA, SNP, CaCl 2 , and ABA priming, with respect to the control and water-treated groups, shortened the average germination time (92% after 5 days) compared to that of the control (80% after 5 days). For HY49 seeds under T 25 W 0 , GA, SNP, CaCl 2 , and ABA priming reduced the average germination time (92% after 5 days) compared to that of the control (85% after 5 days). Similarly, for HY49 seeds under T 15 W 15 , GA priming shortened the average germination time (89% after 5 days) compared to that of the control (83% after 5 days). These priming agents increased the net photosynthesis, stomatal conductivity, and transpiration rate of rape seedlings under conditions of low temperature and drought stress, while also decreasing intercellular carbon dioxide (CO 2 ) concentrations. Additionally, SA, GA, SNP, CaCl 2 , and ABA increased superoxide dismutase concentrations (SOD) and ascorbic peroxidase (APX) activities of rape seedlings under stress conditions, while decreasing catalase (CAT) and peroxidase (POD) activities in ZY15 seedlings. In HY49, which is sensitive to low temperature and drought, all priming solutions, except for SNP, led to an increase in SOD activity levels and a decrease in CAT activity levels. Overall, SA, GA, SNP, and CaCl 2 increased the concentrations of indoleacetic acid (IAA), GA, ABA, and cytokinin (CTK) in seedlings under stress conditions. Moreover, compared to SA, CaCl 2 , and ABA, GA (300 mg/L) and SNP (300 mol/L) showed improved priming effects for ZY15 and HY49 under stress conditions.
研究领域:
植物生理学
·
农业科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）