Nutrients
·
2023
·Vol. 15
·期 19
·4176
·
IF 5.9
Yuxu Chen,Zhiye Zhao,Shancheng Guo,Yaxian Li,Haiaolong Yin,Lei Tian,Guiguang Cheng,Ye Li
Alcoholic liver disease (ALD), leading to the most common chronic liver diseases, is increasingly emerging as a global health problem, which is intensifying the need to develop novel treatments. Herein, our work aimed to estimate the therapeutic efficacy of red rice (Oryza sativaL.) seed coat on ALD and further uncover the underlying mechanisms. Red rice seed coat extract (RRA) was obtained with citric acid–ethanol and analyzed via a widely targeted components approach. The potential targets of RRA to ALD were predicted by bioinformatics analysis. Drunken behavior, histopathological examination, liver function, gut microbiota composition and intestinal barrier integrity were used to assess the effects of RRA (RRAH, 600 mg/kg·body weight; RRAL, 200 mg/kg·body weight) on ALD. Oxidative stress, inflammation, apoptosis associated factors and signaling pathways were measured by corresponding kits, Western blot and immunofluorescence staining. In ALD model mice, RRA treatment increased sphingosine kinase 2 (SPHK2) and sphingosine-1-phosphate (S1P) levels, improved gut microbiota composition, restored intestinal barrier, decreased lipopolysaccharide (LPS) levels in plasma and the liver, cut down Toll-like receptor 4 (TLR4)/Nuclear factor kappa B (NF-κB) pathways, alleviated liver pathological injury and oxidative stress, attenuated inflammation and apoptosis and enhanced liver function. To sum up, RRA targeting SPHK2 can ameliorate ALD by repairing intestinal barrier damage and reducing liver LPS level via the TLR4/NF-κB pathway and intestinal microbiota, revealing that red rice seed coat holds potential as a functional food for the prevention and treatment of ALD.Keywords:red rice seed coat;alcoholic liver disease;SPHK2;gut microbiota;intestinal barrier
研究领域:
药理学
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胃肠病学
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微生物学
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营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SOUTH AFRICAN JOURNAL OF BOTANY
·
2020
·
IF 1.792
Hanzhi Liang,Yuping Xiong,Beiyi Guo,Haifeng Yan,Shuguang Jian,Hai Ren,Songjun Zeng,Kunlin Wu,Jaime A. Teixeira da Silva,Youhua Xiong,Guohua Ma
Scaevola sericea (Gaertn.) Roxb., which is a semi-mangrove halophyte, displays low seed germination (SG) and poor development. A model system was established to assess seed viability (SV) and enhanced SG in response to 2,3,5-triphenyl-tetrazolium chloride (TTC), and to detect SV under different storage conditions and duration to establish an optional storage method for seeds. Percentage SG (SG%) was also tested with different periods of soaking in water, gibberellic acid (GA 3 ) and seawater after removing fruit pulp. Optimal detection of the percentage of SV (SV%) was possible when seeds were soaked for 3 h in 0.4% TTC. In these conditions, after 15 d, SV% exceeded 90.0%. This indicates that low SG was not related to SV under natural conditions. The nutritious epicarp (pulp) of intact fruit is easily infected by mildew, rotting rapidly in high relative humidity (60–90%). When pulp was removed from fruits, germination period was speeded up by almost 8 d (seeds germinated after sowing for 28 d). This also increased SG%. Seawater broke seed dormancy more efficiently than GA 3 . The SG% (59.0%) of seeds with their epicarp removed then soaked in seawater for 24 h was higher than seeds soaked in distilled water (24.0%) or in 200 mg/L GA 3 (33.7%) after sowing for 40 d The SV% of seeds stored at 4 °C was higher than seeds stored at room temperature (25±3 °C), but when storage period was extended to 180 d or longer, their SV% declined rapidly. In natural conditions, S. taccada seeds have a high SV%. The low SG% is caused by easily rotting pulp and the physiological dormancy of fruits. By removing the pulp and soaking naked seeds in seawater can break seed dormancy and achieve ideal SG which would benefit ecorestoration and landscaping projects.
研究领域:
植物学
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生态学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Haiyong Pan,Bai Miao,Wenting Zheng,Lina Yang,He Liu
Polysaccharides derived from soybean seed coats exhibit diverse functional properties and significant application potential; however, their intestinal absorption and transport mechanisms remain poorly understood. This study investigates the molecular docking interactions of two soybean seed coat polysaccharides (NMSP and CSSP), extracted using different methods, with the MUC2 protein, and explores their absorption and transport properties using Caco-2 cell models. Molecular dynamics simulations revealed the binding modes and key interaction sites between NMSP, CSSP, and the MUC2 protein. A Caco-2 monolayer model was established to evaluate polysaccharide uptake and transport under varying concentrations, temperatures, and pH conditions. The results demonstrated that the absorption of both polysaccharides was time- and concentration-dependent, while transport analyses revealed that both NMSP and CSSP could traverse the Caco-2 cells, primarily via endocytic pathways. In terms of apparent permeability (Papp) and transport rate, CSSP exhibited moderate absorption, whereas NMSP was classified as poorly absorbed. Moreover, acidic conditions significantly enhanced the transport rates of both polysaccharides. This study provides critical insights into the absorption and transport characteristics of soybean seed coat polysaccharides, offering a theoretical foundation for further exploration of their bioavailability and physiological functions.
研究领域:
食品科学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2023
·Vol. 12
·期 21
·4005
·
IF 5.2
He Wang,Yue Zheng,Xinyue Tang,Ting Zhang
Loquat is a nutrient-rich fruit with juicy and sweet pulp, but it is vulnerable to rot and deterioration without proper postharvest preservation measures. This study aimed to improve the postharvest quality of loquat by developing a microemulsion system based on an essential oil extracted from theTorreya grandiscv. Merrillii aril (TaEO), which has antimicrobial and antioxidant properties. An optimal TaEO microemulsion (TaEO-ME) was formulated, using a mixture of Tween-40 and Tween-80 as the surfactant, 1-butanol as the co-surfactant, and TaEO as the oil phase, with mass ratios of 9:1, 3:1, and 6:1, respectively. Two TaEO-ME formulations with 60% and 70% water contents were stable for 180 days at room temperature, with a mean droplet size below 12 nm and polydispersity index less than 0.24. They also exhibited higher stability and enhanced biological activities compared to free TaEO. Loquat fruit treated with TaEO-ME displayed a reduced decay index and lower membrane lipid peroxidation after 15 days of storage at 15 °C, as indicated by the lower malondialdehyde content and higher peroxidase activity. Moreover, the TaEO-ME treatment preserved the nutrient quality by maintaining the total phenolic compounds and ascorbic acid content. Our findings suggested that TaEO-ME can be used as a substitute for chemical preservatives to keep fruits fresh.Keywords:microemulsion;Torreya grandiscv. Merrillii aril;essential oil;loquat;fruit quality
研究领域:
食品科学
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植物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2022
·
IF 7.425
Min Lu,Qi Zhou,Hui Yu,Xiaoe Chen,Gaofeng Yuan
Chitosan/gelatin (CG) based intelligent pH-sensing indicator films were fabricated by incorporating zinc oxide nanoparticles (nano-ZnO) (CGZ) and different amounts of black peanut seed coat anthocyanins (BPCSA) (CGZ-h). Chitosan/gelatin showed good compatibility with anthocyanins and nano-ZnO, and the addition of nano-ZnO and BPSCA did not affect the original crystal structure of films. Addition with BPSCA simultaneously increased the tensile strength and elongation at break of CGZ-h films. BPSCA decreased the water vapor permeability, whereas they increased the UV barrier property of the CGZ-h films. CGZ-h films exerted strong antioxidant and antibacterial activities, which mainly attributed to the BPSCA and nano-ZnO, respectively. CGZ-h films presented good pH-sensing color-change properties in different buffer solutions. The CGZ-h2 film was used to monitor the freshness of shrimp at 4 °C. The result showed that distinctive color change of the CGZ-h2 film was highly correlated with the change of the total volatile basic nitrogen, pH and total viable count of shrimp. These results revealed the potential application of CGZ-h for real-time monitoring the freshness of food.
研究领域:
食品科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
PeerJ
·
2018
·
IF 2.118
Faheem Afzal Shah,Jun Ni,Jing Chen,Qiaojian Wang,Wenbo Liu,Xue Chen,Caiguo Tang,Songling Fu,Lifang Wu
Sapium sebiferum , an ornamental and bio-energetic plant, is propagated by seed. Its seed coat contains germination inhibitors and takes a long time to stratify for germination. In this study, we discovered that the S. sebiferum seed coat (especially the tegmen) and endospermic cap (ESC) contained high levels of proanthocyanidins (PAs). Seed coat and ESC removal induced seed germination, whereas exogenous application with seed coat extract (SCE) or PAs significantly inhibited this process, suggesting that PAs in the seed coat played a major role in regulating seed germination in S. sebiferum . We further investigated how SCE affected the expression of the seed-germination-related genes. The results showed that treatment with SCE upregulated the transcription level of the dormancy-related gene, gibberellins (GAs) suppressing genes, abscisic acid (ABA) biosynthesis and signalling genes. SCE decreased the transcript levels of ABA catabolic genes, GAs biosynthesis genes, reactive oxygen species genes and nitrates-signalling genes. Exogenous application of nordihydroguaiaretic acid, gibberellic acid, hydrogen peroxide and potassium nitrate recovered seed germination in seed-coat-extract supplemented medium. In this study, we highlighted the role of PAs, and their interactions with the other germination regulators, in the regulation of seed dormancy in S. sebiferum .
研究领域:
植物学
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植物生理学
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分子生物学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF PLANT PHYSIOLOGY
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2022
·
IF 3.686
Shuangxi Zhang,Huachun Sheng,Yue Ma,Yanping Wei,Dan Liu,Yanhua Dou,Huiying Cui,Boyou Liang,Johannes Liesche,Jisheng Li,Shaolin Chen
Cell wall biogenesis is required for the production of seeds of higher plants. However, little is known about regulatory mechanisms underlying cell wall biogenesis during seed formation. Here we show a role for the phosphorylation of Arabidopsis cellulose synthase 1 (AtCESA1) in modulating pectin synthesis and methylesterification in seed coat mucilage. A phosphor-null mutant of AtCESA1 on T166 ( AtCESA1 T166A ) was constructed and introduced into a null mutant of AtCESA1 ( Atcesa1-1 ). The resulting transgenic lines showed a slight but significant decrease in cellulose contents in mature seeds. Defects in cellulosic ray architecture along with reduced levels of non-adherent and adherent mucilage were observed on the seeds of the AtCESA1 T166A mutant. Reduced mucilage pectin synthesis was also reflected by a decrease in the level of uronic acid. Meanwhile, an increase in the degree of pectin methylesterification was also observed in the seed coat mucilage of AtCESA1 T166A mutant. Change in seed development was further reflected by a delayed germination and about 50% increase in the accumulation of proanthocyanidins, which is known to bind pectin and inhibit seed germination as revealed by previous studies. Taken together, the results suggest a role of AtCESA1 phosphorylation on T166 in modulating mucilage pectin synthesis and methylesterification as well as cellulose synthesis with a role in seed development.
研究领域:
植物生物学
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分子遗传学
·
细胞壁生物合成
✓ 已验证
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Xu Zhang,Xiaozhuo Sun,Yelinxin Chen,Fangzhu Zheng,He Ban,Jingsheng Liu,Dan Cai,Lin Xiu
Curcumin, a bioactive compound characterized by its limited solubility in water and low bioaccessibility, which requires an effective delivery system to enhance stability and absorption. This research focused on creating Pickering emulsions stabilized by zein-adzuki bean seed coat polyphenol-sodium alginate composite nanoparticles (ZAS) to improve curcumin delivery. The effects of different concentrations of sodium alginate (SA) on the properties of ZAS were investigated. Fourier transform infrared spectroscopy (FTIR) showed that there were hydrophobic and hydrogen bonds interactions between SA and nanoparticles, and Differential Scanning Calorimetry demonstrated an improvement in thermal stability. Accelerated oxidation experiments showed that ZAS stabilized Pickering emulsions had good anti-oxidant capacity. In comparison to the control group, the addition of SA increased the loading rate of curcumin to 81.65 ± 1.56 % with improved bioaccessibility (27.99 ± 0.26 %) and in vitro digestion stability (55.72 ± 0.22 %). The findings indicate that ZAS nanoparticles modified with SA markedly enhance the stability of Pickering emulsions, while also attaining a high loading efficiency of 81.65 % and bioaccessibility of 27.99 %. This suggests their potential utility in functional food applications.
研究领域:
食品科学
·
生物化学
·
药物递送系统
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FOOD SCIENCE
·
2025
·Vol. 90
·期 1
·e17604
·
IF 3.4
Yujiao Zhang,Li Zhang,Like Lin,Yiran Zhang,Cong Li,Bang Chen,Yehua Shen
The purpose of this article is to investigate the effects of walnut ( Juglans regia L.) kernel pellicle on the composition and properties of enzymatic hydrolysis products of walnut meal using peptidomics and bioinformatics. In this study, a total of 3423 peptide sequences were identified in peeled walnut protein hydrolysates (PWPH) and unpeeled walnut protein hydrolysates (UWPH). Due to the presence of the walnut kernel pellicle, the enzyme cleavage sites of alkaline proteases on walnut precursor proteins were altered, resulting in differences in the number and length of the peptides obtained. Principal component analysis indicates significant differences between PWPH and UWPH. Combined with bioinformatics analysis, it was shown that walnut kernel peeling improved the release of peptides, formed more bioactive peptides, reduced allergenicity, and improved water solubility. Seven peptides with acetylcholinesterase (AChE) inhibitory activity were identified, and the peptide Val-Gly-Ala-Pro-Phe-Asp-Gly-Ala (VGAPFDGA) has the strongest inhibitory activity with an IC 50 of 0.38 ± 0.01 mg/mL. These results confirmed that walnut kernel peeling could greatly change the composition of the walnut protein hydrolysates, and seven novel peptides were reported that showed significant AChE inhibitory activity.
研究领域:
食品科学
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生物化学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Xiaohan Wang,Bing Li,Shanshan Sun,Qingqing Liu,Jin Zhu,Xian Zhou,Huijin Zhang,Qian Wu,Liangsheng Wang
Antique Lotus ( Nelumbo ) is a perennial aquatic plant with unique historical significance and cultural value, whereas its potential economic value hasn’t been fully explored. The present study showed that lotus seedpods had significantly higher antioxidant capacity than other parts by FRAP, ABTS, and ORAC assays and analyzed the proanthocyanidins and flavonols in the seedpods of Antique Lotus. Polyphenols contributed to great antioxidant activity and 51 polyphenols were identified by UPLC-TQ-MS analysis. In which, 27 compounds were identified from lotus seedpods for the first time, including 20 trimers, 5 dimers and 2 tetramers of proanthocyanidin. Total proanthocyanidins explained 70%–90% of the different antioxidant activities and the content of proanthocyanidin trimers showed the strongest correlations with the antioxidant activities. This study provided a fundamental reference for the research of polyphenols in lotus and found that Antique Lotus seedpod extracts have the promising prospects of additives used in feed and food processing.
研究领域:
植物学
·
植物化学
·
食品科学
·
农业化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Quanyu Zhang,Youling Feng,Hanyu Liu,Hao Zhang,Zhixun Ma,Xin Du,Xiufang Xia
Black soybean seed coat anthocyanins (BBSACN) have garnered considerable attention for their strong antioxidant and health-promoting properties, yet their inherent instability severely hinders practical applications. Cyclodextrin metal-organic frameworks (CD-MOFs) are commonly utilized for safeguarding and delivering active compounds. However, their susceptibility to damage in aqueous environments restricts the efficient loading of hydrophilic active ingredients. This study aimed to create a cross-linked CD-MOF (CLCD-MOF) system to enhance the stability of BBSACN under diverse conditions. Diphenyl carbonate was used as the crosslinker to generate a CLCD-MOF that maintained structural stability in water for 7 days. Thorough physicochemical characterization, including SEM, FTIR, XRD, and TGA, and molecular dynamics simulations verified the successful encapsulation of anthocyanins. The findings indicated that non-covalent interactions such as hydrogen bonding and hydrophobic forces notably enhanced the stability of anthocyanins during extended storage, heating, and light exposure. In comparison to free anthocyanins, those encapsulated in CLCD-MOF showed about 35 % higher retention under light exposure and even greater improvements during storage and heating. In conclusion, this study presents promising opportunities for utilizing CD-MOF carriers to tackle the increasingly intricate challenges in the food industry.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Yue Qiu,Jiayan Xie,Jing Liang,Jianhua Xie,Yi Chen,Xiaobo Hu,Nan Sun,Xiling Wang,Qiang Yu
In this paper, the optimal conditions for extracting dietary fiber-bound polyphenols in Canavalia gladiata seed coat using three hydrolysis methods, namely alkali, acid and enzyme, were obtained using response surface methodology. The total phenolic content of alkali hydrolysis products was 2635.97 μg GAE/g DW, which was much higher than that of the acid (1418.87 μg GAE/g DW) and the enzyme method (1316.39 μg GAE/g DW). Qualitative analysis identified a total of 38 compounds, 33 of which were quantified. The results showed that CGDF-BPs were dominated by phenolic acids, especially gallic acid. Overall the alkaline method hydrolyzed dietary fibers more thoroughly, releasing a higher diversity of compounds. However, their in vitro antioxidant and α-glucosidase inhibitory activities were inconsistent at equivalent concentration due to differences in bound polyphenol composition caused by the alkali, acid, and enzyme methods, which served as a reference for studying of the composition and activity of natural plant polyphenols.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）