JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2026
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IF 5.3
Huan Liu,Liying Liang,Minggang Wang,Qinfeng Huang,Yu Pan,Xiaojie Wei,Wenchao Zhang
The naturally occurring pentacyclic triterpenoid compounds ursolic acid (UA) and oleanolic acid (OA) are commonly found together in various fruits and find broad applications in pharmaceuticals, cosmetics, food and agricultural fields. In this study, a sensitive and practical icELISA method was developed based on a monoclonal antibody (mAb) YC23-101, with a median inhibitory concentration (IC 50 ) of 15.56 ng/mL and a linear range of 5.26 to 54.1 ng/mL. The limits of detection (LOD) ranges from 0.98-5.11 ng/mL in fruit matrices and the recoveries were 94.03-112.21% with coefficients of variation below 5.73%. Cross-reactivity analysis showed that mAb YC23-101 could also specifically recognize OA with similar sensitivity to UA, indicating feasibility for the simultaneously quantification of UA and OA in fruits. The developed icELISA was applied to several fruits, and the results showed good correlation with that of HPLC. Molecular docking analysis predicted equal binding energy of UA and OA to the mAb with hydrophobic interactions playing a predominant role. All these findings demonstrated that this icELISA is reliable, sensitive, and suitable for the high-throughput quantification of total UA and OA content in fruits composition analysis.
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DOI
高影响因子
FREE RADICAL BIOLOGY AND MEDICINE
·
2025
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IF 8.2
Chuanyong Zhao,Fanghui Wu,Liben Zhao,Jiangtao Zhong,Chenhao Huang,Zhangchi Jiang,Jingqi Zheng,Shengwu Yang,Chunwu Zhang,Lei Zhang
Osteoporosis is typified by increased bone degradation mediated through osteoclast activity, necessitating innovative interventions. This research explores the therapeutic efficacy of corosolic acid (CA), a triterpenoid recognized for its antioxidative capacity, emphasizing its mechanism through binding to RANKL to inhibit osteoclast development and reinforce redox balance. High-throughput transcriptome sequencing assessed CA's effects on osteoclast gene networks during differentiation. Combined molecular docking and RANKL-induced signaling cascade assessment elucidated CA's molecular regulatory mechanism governing RANKL-activated osteoclastogenesis. CA impeded RANKL-stimulated differentiation of bone marrow-derived macrophages (BMMs) into osteoclasts and disrupted F-actin ring assembly without cytotoxicity. At the molecular level, CA interfered with RANKL-initiated activation of the canonical NF-κB and MAPK pathways, evidenced by suppressed phosphorylation of IκB-α, ERK, JNK, and p38, and concurrent downregulation of osteoclast-specific transcriptional markers. Moreover, CA restricted NFATc1 translocation into the nucleus. In addition to inhibiting osteoclastogenesis, CA modulated oxidative stress by curbing reactive oxygen species (ROS) production and augmenting antioxidant enzyme expression. In vivo, using an ovariectomy-induced bone loss model, CA treatment led to notable improvements in trabecular bone architecture. Collectively, these data highlight CA's potential to mitigate osteoclastic bone resorption through targeted RANKL inhibition, suppressing NF-κB/MAPK signaling and redox regulation, underscoring its therapeutic potential for postmenopausal osteoporosis.
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PubMed
高影响因子
Journal of Cleaner Production
·
2023
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IF 11.1
Lu Huang,Yingjie Guo,Tianqi Jin,Ke Yan,Xianglin Liu,Shuyang He,Lin Li,Ye Gong,Junyi Ma,Hang Yu,Zhoumin Lu,Yupeng Liu,Wuzi Dong,Fangxia Yang
Triterpene acids (TTAs) have promising applications in functional food, medicine and other fields. Here, ultrasonic-assisted hydrophobic deep eutectic solvent (HDES) extraction was employed to gain TTAs from loquat leaves. Combining COSMO-SAC model prediction with experimental verification，the HDES, composed of thymol and benzyl alcohol (molar ratio 2:1), was proved to have the highest extraction ability for TTAs. The preliminarily optimized extraction conditions were solid to solvent ratio 1:10 (g/g), temperature 60 °C and extraction time 60 min. Under these conditions, the extraction yield of ursolic acid (the main component of loquat leaf TTAs) was 17.769 ± 0.799 mg/g dry loquat leaf. HDES and TTAs were recovered from HDES extracting solution via gradient elution (60% and 80% ethanol aqueous solution, respectively) based on C18 reverse-phase silica gel column chromatography under low pressure (0.5 MPa). Recovered HDES could be reused for extracting TTAs from loquat leaves directly and had a similar extraction performance compared to fresh HDES. The purity of the obtained crude TTAs sample was 81.18% based on the total contents of maslinic acid (MA), corosolic acid (CA), oleanolic acid (OA) and ursolic acid (UA), with a mass ratio of 11:26:10:53. Crude TTAs sample was further separated by C18 reverse-phase silica gel column chromatography using 80% ethanol aqueous solution as mobile phase under high pressure (15 Mpa). By this mean, CA sample with 69.30% purity (MA to CA mass ratio 7:18) and UA sample with 93.94% purity (OA to UA mass ratio 4:21) were obtained. The in vitro antioxidant ability order of the attained TTAs samples was CA sample > crude TTAs sample > UA sample. This study laid a good scientific basis for applying HDES and C18 reverse-phase silica gel column chromatography in the bioactive component extraction and separation fields. It also provided theoretical basis and technical support for obtainment and utilization of high-purity loquat leaf TTAs in the fields of medicine, functional food, cosmetics and so on.
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高影响因子
危险品化学品经营许可证（带存储）