In this study, a new process of pre-flotation decarbonization‑lead flotation‑zinc flotation was proposed to recover valuable metals from a lead‑zinc ore in Guizhou by beneficiation and to determine a reasonable and economic beneficiation process. The results of the study demonstrate that the novel process aids in the flotation of lead and zinc ores in two ways: (1) pre-decarbonization using MIBC (methyl isobutyl carbinol) and kerosene synergistically achieved the lowest loss of lead recovery of 1.94 % and zinc recovery of 1.14 % in the decarbonized product. (2) the successful separation and retrieval of lead and zinc metals were accomplished via a preferential flotation technique, using several combinations of reagents. This procedure yielded a lead recovery rate of 87.64 % and a zinc recovery rate of 94.09 %.
Neuro-inflammation contributes to neuropathic pain by sensitizing ionic channels. Kinsenoside, a traditional Chinese medicine, has recognized anti-inflammatory properties. However, it remains unclear whether kinsenoside can be used for pain therapy. Network pharmacology analysis revealed that 57 % of its targets are associated with pain, including inflammation and synaptic transmission. The analgesic effects of kinsenoside were confirmed in SNL and formalin rat models, with ED50 values of 47.99 μg and 36.80 μg, respectively. Transcriptome and WGCNA analyses indicated the involvement of cytokine release, anti-inflammatory activity, and synapse enrichment in the blue module. Furthermore, we confirmed that kinsenoside's efficacy was mainly mediated by IL-10 induction, phosphorylation of STAT3, and SOCS3 expression. Pretreatment with kinsenoside significantly inhibited the release of TNF-α, IL-1β, and IL-6. Kinsenoside also attenuated ER stress in both microglia and neural cells. Molecular docking analysis demonstrated significantly high binding energies of IL-10, STAT3, and SOCS3 with MHC. Additionally, whole-cell recordings revealed that bath application of kinsenoside reduced the frequency and amplitude of spinal glutamatergic transmission in a dose-dependent manner. In summary, pharmacological prediction and biological validation collectively indicate that kinsenoside significantly exerts significant analgesic effects by attenuating ER stress and inhibiting inflammatory responses via the IL-10/p-STAT3/SOCS3 axis, precisely regulating spinal glutamatergic transmission for pain relief.
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