JOURNAL OF CHROMATOGRAPHY A
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2015
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IF 4.169
Zaizhi Liu,Huiyan Gu,Lei Yang
Ionic liquids/lithium salts solvent system was successfully introduced into the separation technique for the preparation of two coumarins (aesculin and aesculetin) from Cortex fraxini. Ionic liquids/lithium salts based microwave irradiation pretreatment followed by ultrasound-microwave synergy extraction (ILSMP-UMSE) procedure was developed and optimized for the sufficient extraction of these two analytes. Several variables which can potentially influence the extraction yields, including pretreatment time and temperature, [C 4 mim]Br concentration, LiAc content, ultrasound-microwave synergy extraction (UMSE) time, liquid–solid ratio, and UMSE power were optimized by Plackett–Burman design. Among seven variables, UMSE time, liquid–solid ratio, and UMSE power were the statistically significant variables and these three factors were further optimized by Box–Behnken design to predict optimal extraction conditions and find out operability ranges with maximum extraction yields. Under optimum operating conditions, ILSMP-UMSE showed higher extraction yields of two target compounds than those obtained by reference extraction solvents. Method validation studies also evidenced that ILSMP-UMSE is credible for the preparation of two coumarins from Cortex fraxini. This study is indicative of the proposed procedure that has huge application prospects for the preparation of natural products from plant materials.
研究领域:
植物化学
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天然产物分离
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药用植物学
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DOI
PubMed
高影响因子
Separations
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2023
·Vol. 10
·期 9
·515
·
IF 2.6
Xiaodong Wang,Wenhao Wang,Qinggui Lei,Zhengming Qian,Wenbin Deng
This study aims to develop a fast and eco-friendly liquid chromatography–mass spectrometry (LC-MS) method for the determination of aesculin and aesculetin in Cortex Fraxini. Ultrapure water was used as the solvent during the microwave-assisted extraction process to prepare the Cortex Fraxini sample. This extraction method reduces the cost of the harmful solvent (only ultrapure water was used) and microwave extraction time (1 min). The LC separation was conducted using an Agilent InfinityLab Poroshell 120 EC-C18column (2.1 mm × 30 mm, 2.7 µm) with a mobile phase consisting of 0.1% formic acid and acetonitrile (90:10,v/v) at a flow rate of 0.6 mL/min. Isocratic elution was employed, and the analytes were detected by MS. Through careful optimization and selection of LC-MS conditions, the analysis time was reduced to 1 min, demonstrating the method’s efficiency. The developed method was validated and exhibited excellent specificity, linearity, limit, precision, accuracy, and stability in quantifying aesculin and aesculetin in the Cortex Fraxini samples. The analysis result revealed the presence of aesculin (ranging from 3.55 to 18.8 mg/g) and aesculetin (ranging from 1.01 to 16.2 mg/g) in all ten batches of Cortex Fraxini samples. Compared to the reported LC methods, this approach substantially reduces the total analysis time and requires a minuscule volume of organic solvents. An “Analytical Eco-Scale” assessment was used to evaluate the different assay methods of Cortex Fraxini. The current LC-MS method scored an impressive 90; it was better than the other four reports’ LC methods. Thus, the developed LC-MS method is rapid and green, which is helpful for the quality evaluation of Cortex Fraxini.Keywords:LC-MS;Cortex Fraxini;aesculin;aesculetin;green;fast
研究领域:
药学
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
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2023
·
IF 5.4
Yong-Yu Yang,Jing-Jing Qi,Si-Yi Jiang,Ling Ye
Ethnopharmacological relevance Cortex fraxini (also known as qinpi)—the bark of Fraxinus rhynchophylla Hance (Oleaceae)—is widely used as a Chinese traditional medicinal for its anti-inflammatory and anti-hyperuricemic activities. Aim of the study Obesity-induced insulin resistance (IR) is driving the rising incidence of type 2 diabetes mellitus and is related to pathological adipose tissue remodeling. Esculin, a major active component of Cortex fraxini , has anti-diabetic effects. However, whether esculin improves obesity-induced IR by regulating adipose tissue remodeling is unclear. The aims of the present study were to assess the effects of esculin on obesity-induced IR and to explore the underlying mechanisms. Materials and methods Obese IR C57BL/6J mice were treated with esculin (40 or 80 mg/kg/day) for 4 weeks. Oral glucose tolerance tests were used to assess insulin sensitivity. Histological analyses were performed to analyze the number and size distribution of adipocytes. Glucose uptake was assessed using 2-NBDG. Results Esculin had no effect on body weight gain but reduced fasting blood glucose, improved oral glucose tolerance, and increased insulin sensitivity. Esculin reduced adipocyte size and the expression levels of collagen 4A1 and tumor necrosis factor α and increased the number of adipocytes and the expression of vascular endothelial growth factor A. Esculin promoted the differentiation of 3T3-L1 cells and upregulated the mRNA expression of CCAAT/enhancer-binding protein α and peroxisome proliferator-activated receptor-γ, activated the insulin receptor substrate 1 (IRS1)/phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) signaling pathway, and enhanced the translocation of glucose transporter type 4 (GLUT4) and glucose uptake in adipocytes treated with palmitic acid. Conclusions These data suggest that esculin increases insulin sensitivity by improving adipose tissue remodeling and activating the IRS1/PI3K/AKT/GLUT4 pathway.
研究领域:
药理学
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内分泌学
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代谢
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中药
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Xin Yuan,Jia-Hui Liao,Gui-Jia Du,Yi Hou,Song-Qing Hu
Kinsenoside is a rare and valuable glycoside with extensive bioactivities. However, the enzymatic synthesis of kinsenoside has been a challenging task due to the limited enzyme toolbox and unsatisfactory yield. Herein, the β-glucosidase from Dictyoglomus thermophilum (DtBGL) was heterologously expressed, purified and enzymatically characterized. The purified DtBGL was successfully immobilized on the metal-organic frameworks of UiO-66-NH 2 . The DtBGL@UiO-66-NH 2 was fully characterized using SEM, XRD, TGA and FTIR. The studies on enzymatic properties demonstrated that DtBGL@UiO-66-NH 2 exhibited increased catalytic activity and stability compared to the free DtBGL. Particularly, DtBGL@UiO-66-NH 2 could catalyze the synthesis of kinsenoside via the reverse hydrolysis reaction and the kinsenoside yield was 34.12 % under the optimized catalytic system, which was 1.9-fold higher compared with the free DtBGL. Moreover, DtBGL@UiO-66-NH 2 displayed good reusability with a kinsenoside yield of 27.02 % after reuse for 3 times. The present work not only identifies and characterizes a highly active β-glucosidase with reverse hydrolysis activity, but also proposes the immobilized enzyme as an effective catalyst for the industrial production of glycosides.
研究领域:
生物化学
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酶学
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生物催化
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材料科学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL IMMUNOPHARMACOLOGY
·
2024
·
IF 4.8
Feng-Ling Tang,Li-Wei Xie,Lin-Feng Tang,Hai-Yan Lu,Rui-Qiu Zhu,Di-Fan Wang,Ye Tian,Shang Cai,Ming Li
The small intestine exhibits remarkable sensitivity to ionizing radiation (IR), which significantly hampers the effectiveness of radiotherapy in the treatment of abdominal and pelvic tumors. Unfortunately, no effective medications are available to treat radiation-induced intestinal damage (RIID). Fraxin (7-hydroxy-6-methoxycoumarin 8-glucoside), is a coumarin derivative extracted from the Chinese herb Cortex Fraxini. Several studies have underscored the anti-inflammatory, antibacterial, antioxidant, and immunomodulatory properties of fraxin. However, the efficacy of fraxin at preventing or mitigating RIID remains unclear. Thus, the present study aimed to investigate the protective effects of fraxin against RIID in vitro and in vivo and to elucidate the underlying mechanisms. The study findings revealed that fraxin markedly ameliorated intestinal injuries induced by 13 Gy whole abdominal irradiation (WAI), which was accompanied by a significant increase in the population of Lgr5 + intestinal stem cells (ISCs) and Ki67 + progeny. Furthermore, fraxin mitigated WAI-induced intestinal barrier damage, and reduced oxidative stress and intestinal inflammation in mice. Transcriptome sequencing of fraxin-treated mice revealed upregulation of IL-22, a pleiotropic cytokine involved in regulating the function of intestinal epithelial cells. Moreover, in both human intestinal epithelial cells and ex vivo cultured mouse intestinal organoids, fraxin effectively ameliorated IR-induced damage by promoting the expression of IL-22. The radioprotective effects of fraxin were partially negated in the presence of an IL-22-neutralizing antibody. In summary, fraxin is demonstrated to possess the ability to alleviate RIID and maintain intestinal homeostasis , suggesting that fraxin might serve as a strategy for mitigating accidental radiation exposure- or radiotherapy-induced RIID.
研究领域:
放射生物学
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药理学
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分子生物学
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免疫学
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胃肠病学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2019
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IF 3.206
Xiaolan Jiang,Yuncong Yuan,Linbo Chen,Yiming Liu,Mengwei Xiao,Yadong Hu,Ze Chun,Xun Liao
Monoamine oxidase B (MAO-B) was for the first time immobilized on magnetic nanoparticles to be used for screening of MAO-B inhibitors from herbal extracts. The immobilized enzyme was characterized with transmission electronic microscopy , Fourier transform infrared spectroscopy, thermal gravimetric analysis , and vibrating sample magnetometer. The immobilized enzyme retained 95% of the activity, while the thermostability was significantly increased. It was then used as solid phase extraction absorbent to extract MAO-B's ligands from two traditional Chinese medicinal plants, Cortex fraxini and Pericarpium granati . Calceolarioside B and ellagic acid were extracted from C. fraxini and P. granati, respectively, and both of them were found to be inhibitors of MAO-B with IC 50 of 40.95 ± 0.63 and 103.70 ± 2.34 μ M, respectively. This is the first report that calceolarioside B inhibits MAO-B activity. Furthermore, inhibitory mechanisms of both compounds were studied by molecular docking analysis, the results of which demonstrated that the affinity of calceolarioside B with monoamine oxidase B was stronger than that of ellagic acid. The method proposed in this work is highly efficient for screening of MAO-B inhibitors from complex mixtures, showing great potential in discovering anti-Parkinson's disease compounds present in medicinal plants.
研究领域:
药理学
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生物化学
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分子生物学
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中医药学
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产品关联
DOI
高影响因子
European Journal of Wood and Wood Products
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2022
·Vol. 81
·期 3
·685-697
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IF 2.528
Huang Dan,Wang Jing,Li Fanghua,Xie Min,Qu Qingli,Wang Yimin,Sun Wenjuan,Wu Caie,Xu Wenhui,Xiong Ranhua,Ding Yichun,Yang Anquan,Huang Chaobo
In this study, for the first time, optimized phenolic compounds and antioxidant properties were derived from the extraction process of Cortex fraxini (CFR) using response surface methodology. Four factors and three levels of Box-Behnken (BBD) were used to study the extraction time (X 1 ), extraction temperature (X 2 ), ethanol concentration (X 3 ), and solvent-material ratio (X 4 ). The analysis of variance (ANOVA) revealed that temperature and solvent-material ratio had a relatively large effect on the response. The optimal combination of phenolic compounds and antioxidant activity was obtained with the optimal conditions of X 1 = 1.629 h, X 2 = 53.367 ℃, X 3 = 60.971% (v/v), and X 4 = 45.582 mL/g under ultrasonic assisted extraction (UAE). The experimental parameters were consistent with the predicted values. Caffeic acid, erucic acid, ferulic acid and p-hydroxycinnamic acid and other 19 phenolic acids were characterized by high performance liquid chromatography-mass spectrometry (HPLC–MS). The results of this study indicate that optimization of the extraction process is essential for the accurately determined measurement of phenolic components and antioxidants in CFR for its industrial applications.
研究领域:
生药学
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分析化学
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药学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Mengyao Chen,Chunling Yin,Leqian Hu,Xinyao Lin,Liuchuang Zhao,Zhimin Liu,Peng Li
Quality of Cortex Fraxini is closely related to its clinical efficacy and drug safety. Traditional quality assessment methods are either non-objective or time-consuming. It is imperative to establish a rapid method for quality detection and evaluation of Cortex Fraxini . In this study, fourier transform infrared spectroscopy (FT-IR) and near-infrared spectroscopy (NIR) combined with chemometrics were used for rapid quantitative analysis of the main active components in Cortex Fraxini . Partial least squares regression (PLSR) combined with variable select by competitive adaptive reweighted sampling (CARS) algorithm gave rise to good predictions of the content of the three active components with R 2 values greater than 0.98. Furthermore, the quality evaluation results of Cortex Fraxini cannot be simply derived from data on the content of active ingredients. This study used technique for order preference by similarity to an ideal solution (TOPSIS) method and consistency analysis to evaluate the quality of Cortex Fraxini from five geographical origins. Two methods respectively realized the quality superiority ranking and quality consistency grading of Cortex Fraxini samples. The evaluation results showed that Cortex Fraxini derived from SN had the best performance in quality and was consistent with the standard of genuine regional herbs. This study introduces an effective technique for swift identification of the primary active compounds in Cortex Fraxini , as well as for an overall assessment of its quality. The combination of spectroscopic techniques and chemometrics provides a rapid and efficient method for the quality analysis of Cortex Fraxini .
研究领域:
药物分析
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光谱技术
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化学计量学
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中药
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）