BIOCHEMICAL ENGINEERING JOURNAL
·
2016
·
IF 2.463
Xin-Jun Yu,Jie Sun,Ya-Qi Sun,Jian-Yong Zheng,Zhao Wang
In this study, the phytohormone gibberellin was evaluated for its effect on lipid and docosahexaenoic acid (DHA) accumulation in Aurantiochytrium sp.YLH70. It was found that 4 mg/mL of gibberellin increased biomass, lipid and DHA yield of Aurantiochytrium sp.YLH70 by 14.4%, 43.6% and 79.1%, respectively. Moreover, a GC–MS metabolomics method combined with a multivariate analysis was applied to reveal the metabolic mechanisms responsible for the increased lipid and DHA accumulation. The principal component analysis (PCA) revealed that metabolomics profiles from all groups were discriminated. 38 metabolites identified by the partial least-squares-discriminant analysis (PLS-DA) were responsible for responding to gibberellin treatment. Metabolic pathway analysis showed that gibberellin accelerated the rate of utilization of glucose, and metabolites in fatty acids biosynthesis and mevalonate pathway were increased, while metabolites in glycolysis and TCA cycle were decreased in Aurantiochytrium sp.YLH70. Moreover, the anti-stress mechanism in Aurantiochytrium sp.YLH70 might be induced by gibberellin.
研究领域:
生物化学
·
代谢组学
✓ 已验证
DOI
高影响因子
Acta Biomaterialia
·
2019
·
IF 6.638
Yan-Wen Bao,Xian-Wu Hua,Yan-Hong Li,Hao-Ran Jia,Fu-Gen Wu
Lysosomal entrapment and liver accumulation are the two main obstacles faced by many anticancer drugs for achieving satisfactory therapeutic outcomes. Here, we develop a facile one-step hydrothermal synthetic route to prepare trace metal (M)-, N-, and O-doped carbon-dominated nanoparticles (termed as MNOCNPs, M = Ni, Pd, or Cu, metal content: <0.1 mol%) with exceptional photothermal properties (e.g., the ultrahigh extinction coefficient of 32.7 L g −1 cm −1 ), which can simultaneously realize preferable endoplasmic reticulum (ER) targeting and specific tumor enrichment without noticeable liver accumulation after poly(ethylene glycol) (PEG) conjugation. More interestingly, the PEG-modified MNOCNPs with nanoscale lengths exhibit considerable nucleolar delivery and increased tumor accumulation upon laser irradiation. After fluorescence labeling, these PEG-modified MNOCNPs are suitable for fluorescence/photoacoustic/thermal triple-modal imaging-guided photothermal cancer treatment. Additionally, the ultralow metal content ensures the exceptional biosafety of the nanoagents. The present work provides a novel, facile, and general synthetic method of carbon-dominated nanoparticles with superior photothermal properties for highly efficient tumor ablation, and the large-organelle (ER and nucleus)-targeted cancer therapeutic strategy may represent an alternative solution for optimizing the anticancer efficacy of nanomaterials . Statement of Significance Limited wire-like nanomaterials have been used for biomedical applications due to their lack of intrinsic photothermal properties, poor cellular uptake and tumor accumulation, and potential biotoxicity arising from their micrometer lengths and/or massive heavy metal doping. Besides, the clinical applications of many nanoagents are hindered by their tendency to accumulate in liver, which may cause severe liver toxicity. Herein, we develop for the first time a one-step hydrothermal method to prepare wire-like trace metal-, N-, and O-doped carbon-dominated nanoparticles with excellent photothermal properties, massive cellular uptake, preferable ER localization, selective tumor targeting with negligible liver deposition, laser irradiation-enhanced nucleolar delivery and tumor accumulation, and multimodal imaging-guided cancer therapy. This work opens a new window for simultaneously overcoming lysosomal entrapment and liver accumulation in cancer therapy.
研究领域:
生物医学工程
·
肿瘤学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
·
2014
·Vol. 301
·期 1
·257-262
·
IF 1.415
Zha Zhongyong,Wang Dingna,Hong Wei,Liu Li,Zhou Sai,Feng Xiaojie,Qin Bing,Wang Jianmei,Yang Yi,Du Liang,Zhang Dong,Fang Zhendong,Xia Chuanqin
This study aimed at influence of europium speciation on its accumulation in Brassica napus (CK) and over-expressing BnTR1 lines (OE), and the kinetics of Eu uptake were investigated. These results indicated that the uptake in the roots of OE was higher than that of CK at high concentrations of europium and even the enrichment in the roots of OE was as high as 20,000 mg/kg at 328.9 μΜ Eu, this suggested that OE might have better resistance to uranium than CK. The time kinetics in plants showed that there had the similar trend between CK and OE. The formation of Eu 3+ at pH 5 in deionized water was beneficial to plants enrichment Eu and carbonate could reduce the adsorption of Eu in two group and phosphate almost completely impress the adsorption of Eu in two group, but citric acid could enhance europium root-to-shoot translocation in two group. These results would help understanding the mechanism of Eu uptake in Brassica napus (CK) and over-expressing BnTR1 lines (OE), therefore properly developing efficient europium/americium phytoremediation.
研究领域:
植物学
·
环境科学
✓ 已验证
DOI
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2025
·
IF 5.4
Zhaolin Sun,Yuhua Wei,Shuangyi Ma,Yuchen Xu,Yuna Zhang,Changxian Li,Jinghan Zhang,Feifei Duan,Jianwei Jiao,Xiuying Duan
Ethnopharmacological relevance Metabolic dysfunction-associated steatotic liver disease (MASLD) is the most prevalent liver disease worldwide, primarily caused by poor lifestyle choices and dietary habits. Caulis Bambusae In Taenias , commonly known as Zhuru in Chinese, is a traditional Chinese medicinal material from the Bambusoideae family, frequently used for liver detoxification, liver protection, and lowering lipids. Aim of the study The study aimed to reveal the protective effects and underlying mechanisms of Zhuru extracts (EZR) in treating MASLD. Methods High performance liquid chromatography, liquid chromatography-tandem mass spectrometry, and fourier infrared spectroscopy were used to identify the components of EZR. We utilized oleic acid-treated HepG2/LO2 cells as in vitro models and zebrafish on high-fat diets (HFD) as animal models, and conducted network pharmacology, RNA sequencing, and biological assays, including confocal fluorescence, commercial kits, western blotting, and quantitative PCR, to investigate the lipid-lowering effect of EZR and its underlying mechanisms. Results EZR alleviated lipid accumulation in both in vitro and in vivo models. Analyses through network pharmacology and RNA sequencing revealed that the lipid-lowering properties of EZR were associated with pathways related to lipid biosynthesis, antioxidant functions, and anti-inflammatory mechanisms. Subsequent experimental validation confirmed that EZR decreased the expression levels of FASN, ACC1, HMGCR, ACLY, and LDLR. Additional findings indicated that EZR inhibited mTORC1 activation, leading to the suppression of SREBP1 and SREBP2, thereby mitigating lipid accumulation. Furthermore, EZR was found to downregulate oxidative stress and the levels of inflammatory cytokines. Conclusions EZR demonstrated the ability to lower lipid levels and alleviate HFD-induced MASLD. This is achieved by inhibiting mTORC1/SREBPs-related lipid biosynthesis and reducing oxidative stress and inflammation.
研究领域:
药理学
·
肝脏疾病
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2020
·
IF 6.551
Xiaowen Wang,Ajay Jain,Xu Huang,Xiaoxia Lan,Li Xu,Gengmao Zhao,Xin Cong,Zhantian Zhang,Xiaorong Fan,Feng Hu
The uptake and accumulation of polycyclic aromatic hydrocarbons (PAHs) in crops have gained much attention due to their toxicity to humans. Nitrogen (N) is an essential element for plant growth and has also been implicated in the acquisition and acropetal translocation of PAHs. OsNRT2 . 3b encodes a nitrate (NO 3 − ) transporter that is involved in the acquisition and mobilization of N in rice. Here, we investigated whether overexpression of OsNRT2 . 3b would exert any mitigating influence on the uptake and translocation of phenanthrene (Phe, a model PAH) in transgenic rice ( Oryza sativa ). The wild-type seedlings exhibited a reduction in plant height, primary root length, and shoot biomass when grown hydroponically in a medium supplemented with Phe. Acquisition of Phe by the roots and its subsequent translocation to shoots increased concomitantly with an increase in Phe concentration in the medium and duration of the treatment. OsNRT2 . 3b -overexpressing lines (Ox-6 and Ox-8) were generated independently. Compared with the wild-type, the concentration of Phe in Ox-6 and Ox-8 were significantly lower in the roots (47%–54%) and shoots (22%–31%) grown hydroponically with Phe (1 mg/L). Further, the wild-type and Ox lines were grown to maturity in a pot soil under Phe conditions and the concentrations of Phe and total N were assayed in the culms and flag leaves. Compared with the wild-type, in Ox lines the concentration of total N significantly increased in the culms (288%–366%) and flag leaves (12%–25%), while that of Phe significantly reduced in the culms (25%–28%) and flag leaves (18%–21%). The results revealed an antagonistic correlation between the concentration of total N and Phe. The concentration of Phe was also significantly lower (29%–38%) in the seeds of Ox lines than the wild-type. The study highlighted the efficacy of overexpressing OsNRT2 . 3b in mitigating the Phe toxicity by attenuating its acquisition, mobilization, and allocation to the seeds.
研究领域:
植物学
·
环境科学
·
遗传学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2022
·
IF 10.753
Zhaoxue Zhang,Yi Lu,Haipu Li,Ya Gao,Zhaoguang Yang
Rice is one of the world's staple foods . Cadmium (Cd) levels in paddy soil are still increasing, and “Cd-contaminated rice” is a frequent occurrence, posing a serious threat to human health. Therefore, Cd contamination in rice is a key issue in agricultural production that needs to be addressed urgently. The Cd accumulation in rice is closely related to other elements. In this study, the impact of nickel (Ni) on the uptake and accumulation of Cd in rice was revealed, and the mechanism was discussed. Statistical analysis of field data showed that Cd concentration in rice grains decreased exponentially with increasing Ni concentration in paddy soils, which was verified by the hydroponic experiments. Under 5 μmol/L Cd exposure conditions, the addition of Ni (100 μmol/L) reduced the Cd contents in roots, stems, and leaves by 81.6 %, 60.6 %, and 65.9 %, respectively. With the presence of Ni, the amount of iron plaque decreased, and the Cd content in the iron plaque was reduced due to the competition between Ni and Cd for adsorption sites. In addition, the migration of Cd from stems to leaves was reduced. At the same time, the distribution of Cd in the cell was altered, and the concentration of Cd in the root cell walls increased with increasing Ni addition under 5 μmol/L Cd exposure. These findings highlight the critical role of Ni in inhibiting Cd accumulation in rice, and provide important information for understanding the effects of coexisting elements in Cd-contaminated soils on Cd accumulation in crops.
研究领域:
农业科学
·
环境科学
✓ 已验证
DOI
PubMed
高影响因子
Fermentation-Basel
·
2023
·Vol. 9
·期 9
·818
·
IF 3.7
Shuang Zhang,Pan Zhao,Ming Gao,Chuanfu Wu,Qunhui Wang,Xiaohong Sun
Anaerobic digestion (AD) has the advantages of utilizing complex substrates and producing renewable energy and is currently one of the mainstream technologies for food waste (FW) resourcing. However, at high organic loads and low inoculum-to-substrate ratios (ISRs), AD with FW as substrate is prone to acid accumulation, resulting in a drastic decrease in gas production and system collapse. This study investigated the effect of the coupled addition of zero-valent iron (ZVI) and activated carbon (AC) on the AD of FW at three low ISRs of 0.715, 0.625, and 0.5. The results showed that the control group acidified and stopped producing biogas when the ISR decreased to 0.625 and 0.5, but ZVI coupled with AC alleviated the acidification and increased the cumulative biogas yield. Especially at ISR = 0.5, the cumulative biogas yield for the ZVI + AC group was 31.5%, 99.5%, and 11.43 times higher than that of the ZVI, AC, and control groups, respectively. ZVI coupled with AC also increased the degradation of volatile fatty acids (70.5–84.4%) and soluble chemical oxygen demand (50.0–72.9%) while decreasing propionate concentration and improving the stability of the AD system. COD mass balance analyses indicated that the coupled addition of ZVI and AC promoted the conversion of particulate organic matter to soluble organic matter and increased the conversion of carbon sources to methane.Keywords:fermentation;methane production;acidification;acid accumulation
研究领域:
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
ENVIRONMENTAL POLLUTION
·
2020
·
IF 6.793
Tianhao Tang,Xiaochun Liu,Longqian Wang,Achuo Anitta Zuh,Weichuan Qiao,Jun Huang
Bioaccumulation and toxicity of per-and polyfluoroalkyl substances and metal in plants have been confirmed, however their contamination in soil and plants still requires extensive investigation. In this study the combined effects of chlorinated polyfluoroalkyl ether potassium sulfonate (F53B) and chromium (Cr) on water spinach ( Ipomoea aquatica Forsk ) were investigated. Compared with each single stress, the combination of F53B and Cr (VI) reduced the biomass and height and increasingly accumulated in the roots and destroyed the cell structure. Besides, the co-contamination led to the immobilization of F53B and Cr (VI) in soil, which affected their migration in soil and transfer to plants. The antioxidant response and photosynthesis of the plant weakened under the single Cr (VI) and enhanced under the single F53B treatment; however the contamination of F53B and Cr (VI) could also reduce this effect, as confirmed by the gene expression of MTa, psbA and psbcL genes. This study provides an evidence of the environmental risks resulting from the coexistence of F53B and Cr (VI).
研究领域:
环境科学
·
植物学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Soil Science and Plant Nutrition
·
2024
·Vol. 24
·期 2
·4000-4016
·
IF 3.4
Qin Yuyan,Wang Yunru,Shi Pengtao,Wu Feng,Zhong Yunjie,Li Hong,Shan Bin
Currently, co-contamination of arsenic (As) and selenium (Se) is increasingly observed in specific locales, attributable to the expansion of Se-enriched agriculture. This paper aims to elucidate the possible mechanism of As and Se interaction. In this investigation, a soil pot experiment was conducted to explore the individual and combined effects of As (arsenate, 100 mg·kg − 1 soil) and Se (selenite, 7.5 mg·kg − 1 soil) on their accumulation, translocation, speciation composition, and subcellular distribution in three Brassica vegetables. The results showed that As stress remarkably inhibited plant growth. Under As stress, the addition of Se did not significantly affect As transport factors, yet it notably increased total As content in the stems, leaves, and flowers. Se addition was observed to obstruct As conversion from inorganic to organic forms, thereby elevating both As(III) and As(V) levels in the aerial organs. Subcellular analysis revealed an increase in As levels within the leaf cell walls, organelles, and soluble cytosol. Interestingly, the impact of As on Se accumulation was found to be stimulatory in choy sum but inhibitory in pak choi and mustard. Under Se stress, As supplementation impeded the conversion of Se from inorganic to organic forms, leading to higher Se(VI) concentrations. At the subcellular level, As addition promoted Se translocation from the leaf cell wall and organelles to cytosolic, where both As and Se predominantly accumulated. These results suggest that the interaction between As and Se inhibits their metabolic processes and enhances vacuolar compartmentalization, thereby influencing their accumulation in plants. The research highlights the heightened risk of As contamination in Se-enriched vegetables cultivated in As-contaminated soil.
研究领域:
植物学
·
环境科学
·
农业科学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Environmental Sciences
·
2022
·
IF 6.796
Chunfeng He,Jini Zhou,Chen Yang,Zhiyi Song,Junheng He,Ziqing Huang,Yurong Deng,Jinling Wang,Yu Xiong,Zhi Dang
Co-exposure to heavy metal and antibiotic pollution might result in complexation and synergistic interactions, affecting rice growth and further exacerbating pollutant enrichment. Therefore, our study sought to clarify the influence of different Tetracycline (TC) and Cadmium(Cd) concentration ratios (both alone and combined) on rice growth, pollutant accumulation, and transportation during the tillering stage in hydroponic system. Surprisingly, our findings indicated that the interaction between TC and Cd could alleviate the toxic effects of TC/Cd on aerial rice structures and decrease pollutant burdens during root elongation. In contrast, TC and Cd synergistically promoted the accumulation of TC/Cd in rice roots. However, their interaction increased the accumulation of TC in roots while decreasing the accumulation of Cd when the toxicant doses increased. The strong affinity of rice to Cd promoted its upward transport from the roots, whereas the toxic effects of TC reduced TC transport. Therefore, the combined toxicity of the two pollutants inhibited their upward transport. Additionally, a low concentration of TC promoted the accumulation of Cd in rice mainly in the root tip. Furthermore, a certain dose of TC promoted the upward migration of Cd from the root tip. Laser ablation-inductively coupled plasma mass spectrometry demonstrated that Cd mainly accumulated in the epidermis and stele of the root, whereas Fe mainly accumulated in the epidermis, which inhibited the absorption and accumulation of Cd by the rice roots through the generation of a Fe plaque. Our findings thus provide insights into the effects of TC and Cd co-exposure on rice growth.
研究领域:
植物学
·
环境科学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2022
·
IF 10.753
Qunhuan Ma,Lizhao Chen,Li Zhang
The effects of nutrient phosphate (P) at environmentally relevant levels on the toxicity of arsenic (As) in marine microalgae have been rarely known. In the present study, we explored the toxicity and bioaccumulation of As in a globally distributed diatom species Skeletonema costatum at different ambient P concentrations. The results showed that As toxicity was suppressed at elevated P concentrations. Intracellular As content ([As] intra ) and the molar ratio of intracellular As to P ([As:P]) were negatively correlated with the ambient P concentrations. The trends of As bioaccumulation were substantially different between the relatively low (0, 0.5 and 1.5 μM) and high P concentrations (7.5 and 37.5 μM). Both [As] intra and [As:P] suggested that As bioaccumulation was a better factor to explain the As toxicity comparing to the ambient As concentration. The 4 h As uptake kinetics at different P concentrations followed Michaelis–Menten kinetic pattern. The maximum uptake rates (V max ) decreased with the increase in P concentrations, and the half-saturation constants (K d ) remained constant except for that at extremely high P concentration (37.5 μM-P), suggesting the depression of P on As uptake was mainly due to the non-competitive effect. Overall, these results demonstrate that the P concentration in seawater is an important factor affecting As toxicity and bioaccumulation in the marine diatom. This study therefore helps us better understand the effects of eutrophication on the toxicity and biogeochemistry of As in the marine environment.
研究领域:
海洋生物学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2025
·
IF 6.8
Ying-Chun Song,Yu-Qi Zhao,Chi-Ming Tang,Pedro Garcia-Caparros,Si-Yu Yang,Jia-Qi Yu,Xiu-Ting Hu,Kang-Di Hu,Zhuo Han,Hua Zhang,Gai-Fang Yao
Hydrogen sulfide (H 2 S) is a gaseous signaling molecule that extends the postharvest storage period of fruit. Biaxially oriented polyethylene (BOPE) films are widely used in fruit packaging due to their excellent properties. By analyzing the phenotypic characteristics of strawberry fruits by comparing with the control, PE and BOPE or in combination with H 2 S at 4 ℃, it was shown that the H 2 S+BOPE treatment reduced decay and water loss, and maintained the best lustre and firmness than other groups. A total of 7,258 co-differentially expressed genes (DEGs) were identified by comparing H 2 S, H 2 S+PE and H 2 S+BOPE with Control by transcriptome analysis. GO, WGCNA and KEGG analyses showed that DEGs were mainly enriched in starch and sucrose metabolism, pigment metabolism and antioxidant activity from the MEyellow and MEblack modules. Moreover, these candidate genes were screened, and the expression level of CHS , PAL and DFR were significantly decreased in H 2 S+BOPE group than other groups by RT-qPCR. Physiological assessments indicated that H 2 S+BOPE treatment significantly delayed chlorophyll degradation, anthocyanin accumulation and TA content, increased reducing sugar content, carotenoid and POD activity, and maintained low MDA content in strawberries. PCA and heatmap showed that POD and pigments were main factors and the correlation analysis proved that POD activity exhibited a positive correlation with the expression of DFR (0.57) and bHLH3 (0.56), carotenoid content (0.54), and it showed a negative correlation with chlorophyll contents (-0.37). These results indicated that BOPE could effectively improve the appearance and antioxidant activity of strawberry fruits, thereby prolonging their postharvest storage period.
研究领域:
植物学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）