BIORESOURCE TECHNOLOGY
·
2015
·
IF 4.494
Hongsen Zhang,Jian Zhang,Jie Bao
This study reported a high titer gluconic acid fermentation using dry dilute acid pretreated corn stover (DDAP) hydrolysate without detoxification. The selected fermenting strain Aspergillus niger SIIM M276 was capable of inhibitor degradation thus no detoxification on pretreated corn stover was required. Parameters of gluconic acid fermentation in corn stover hydrolysate were optimized in flasks and in fermentors to achieve 76.67 g/L gluconic acid with overall yield of 94.91%. The sodium gluconate obtained from corn stover was used as additive for extending setting time of cement mortar and similar function was obtained with starch based sodium gluconate. This study provided the first high titer gluconic acid production from lignocellulosic feedstock with potential of industrial applications.
研究领域:
生物技术
·
发酵工程
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DOI
PubMed
高影响因子
GREEN CHEMISTRY
·
2022
·Vol. 24
·期 24
·9595-9601
·
IF 11.034
Zhenyu Zhang,Anran Li,Haining Na,Fei Liu,Jin Zhu
Fibrillated cellulose with outstanding mechanical strength, biocompatibility, biodegradability and a high specific surface area has attracted widespread attention from researchers. Mechanical fibrillation is the most common preparation method, but a complex pretreatment and high energy consumption are inevitably required. In this study, amino-enriched g-C3N4 as both a relatively weak hydrogen bond donor and a solid base was used in MCF preparation. Almost all kinds of cellulose (without pretreatment) can be directly converted into MCF with a diameter of 200–300 nm with the synergistic action of g-C3N4 and acid (0.02 mol L−1 H2SO4) in 10 min under microwave irradiation. The length of the micro-fibrillated cellulose fiber (MCF) is consistent with the size of the cellulose crystalline region in raw materials. Furthermore, the obtained MCF successfully achieved real-time adsorption of a disperse blue dye and the residue contains a large amount of g-C3N4, which can be used for efficient photocatalytic degradation of rhodamine B. This research, into breaking the hydrogen bonds between fibril bundles by solid–solid interactions rather than mechanical fibrillation, provides a new strategy for MCF preparation.
研究领域:
材料科学
·
化学工程
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
·
2016
·
IF 1.114
Xue-Fang Chen,Chao Huang,Lian Xiong,Bo Wang,Gao-Xiang Qi,Xiao-Qing Lin,Can Wang,Xin-De Chen
Elephant grass (Pennisetum purpureum) dilute acid hydrolysate contains 34.6 g/L total sugars. The potential of lipid production by oleaginous yeast Trichosporon cutaneum grown on elephant grass acid hydrolysate was investigated for the first time. During the fermentation process on the elephant grass acid hydrolysate, glucose, xylose, and arabinose could be well utilized as carbon sources by T. cutaneum. Interestingly, xylose was almost no use before glucose was consumed completely. This illustrated that simultaneous saccharification of xylose and glucose by T. cutaneum did not occur on elephant grass acid hydrolysate. The highest biomass, lipid content, lipid yield, and lipid coefficient of T. cutaneum were measured after the sixth day of fermentation and were 22.76 g/L, 24.0%, 5.46 g/L, and 16.1%, respectively. Therefore, elephant grass is a promising raw material for microbial oil production by T. cutaneum.
研究领域:
生物技术
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENERGY CONVERSION AND MANAGEMENT
·
2015
·
IF 4.38
Zhengqiu Yuan,Jinxing Long,Tiejun Wang,Riyang Shu,Qi Zhang,Longlong Ma
Enhancement of the cellulose accessibility is significant for biomass enzymatic hydrolysis. Here, we reported an efficient combined pretreatment for corn straw enzymolysis using ball milling and dilute acid hydrothermal method (a mixture solvent of H 2 O/ethanol/sulfuric acid/hydrogen peroxide liquid). The process intensification effect of ball milling on the pretreatment of the corn straw was studied through the comparative characterization of the physical–chemical properties of the raw and pretreated corn straw using FT-IR, BET, XRD, SEM, and HPLC analysis. The effect of the pretreatment temperature was also investigated. Furthermore, various pretreatment methods were compared as well. Moreover, the pretreatment performance was measured by enzymolysis. The results showed that ball milling had a significant process intensification effect on the corn straw enzymolysis. The glucose concentration was dramatically increased from 0.41 to 13.86 mg mL −1 after the combined treatment of ball milling and hydrothermal. The efficient removal of lignin and hemicellulose and the enlargement of the surface area were considered to be responsible for this significant increase based on the intensive analysis on the main components and the physical–chemical properties of the raw and pretreated corn straw.
研究领域:
生物化学
·
生物质能
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DOI
高影响因子
iScience
·
2023
·
IF 5.8
Lingjun Zhu,Hao Xu,Xiaoyan Yin,Shurong Wang
With hydrothermal reaction, lignocellulosic biomass can be efficiently converted into furfural (FF) and levulinic acid (LA), both of which are key platform compounds that can be used for the subsequent preparation of aviation fuels. In order to reduce the acid concentration in traditional hydrolysis and provide a reaction system with good catalytic activity, we propose a biomass conversion route as dilute acid hydrolysis coupled with solid acid catalysis. Firstly, at different temperatures, the hemicellulose and cellulose in corn stover were step-hydrolyzed by sulfuric acid solution with a concentration of 0.9 wt. % to produce xylose and glucose, with conversion reaching 100% and 97.3%, respectively. Subsequently, a new resin-derived carbon-based solid acid catalyst was used to catalyze the aforementioned saccharide solutions to obtain FF with yield of 68.7 mol % and LA of 70.3 mol %, respectively. This work provides a promising approach for the efficient production of bio-aviation fuel precursors.
研究领域:
生物化学
·
可再生能源
✓ 已验证
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2015
·
IF 4.494
Jinping Ni,Na Teng,Haizhen Chen,Jinggang Wang,Jin Zhu,Haining Na
This work studied the hydrolysis behavior of regenerated celluloses (RCs) with different degree of polymerization (DP) by using the catalyst of dilute acid under microwave radiation. Results showed that the DP had a considerable influence on hydrolysis of cellulose. The reactivity of RCs was significantly improved when DP was lower than 51. The highest sugar yield of 59.2% was achieved from RC with lowest DP of 23 at 160 °C for 15 min. But the lowest yield of 32.6% was obtained when RC with highest DP of 132 was used. Recrystallization of cellulose was found to hinder the further hydrolysis particularly with the high DP. The effect of recrystallization can be reduced by the decrease of DP of RCs. This research demonstrates that the DP of RCs plays a crucial role on hydrolysis and it provides a preliminary guide based on DP to find a suitable pretreatment method for cellulose hydrolysis.
研究领域:
生物化学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
BIOPROCESS AND BIOSYSTEMS ENGINEERING
·
2023
·Vol. 46
·期 4
·589-597
·
IF 3.8
Jiang Wenfei,Dai Lin,Tan Xin,Zhou Xin,Xu Yong
Pre-hydrolysate liquor, as an inevitable by-product, contains a large amount of xylose, and is therefore an inexpensive feedstock that can be upgraded to value-added chemical xylonic acid. However, inhibitors, simultaneously formed in lignocellulose pretreatment process, are regarded as the major obstacle for effectively bio-converting xylose in pre-hydrolysate into xylonic acid. In this study, Gluconobacter oxydans , with highly selective and efficient, was employed for xylonic acid production; the impacts of five typical toxic inhibitory compounds on xylonic acid productivity and the activity of the membrane-bound dehydrogenase were evaluated. The results revealed that the inhibitors showed different degrees of influence toward xylonic acid production, and the order of inhibitory effect for acidic inhibitors was formic acid > acetic acid > levulinic acid; the inhibitory effect of aldehyde inhibitors was furfural > 5-hydroxymethyl-furfural. This study provides an important basis of metabolic modification and detoxification process for enhancing inhibitor tolerance and xylonic acid productivity. Graphical abstract
研究领域:
生物工程
·
发酵工程
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产品关联
DOI
PubMed
高影响因子
Journal of Water Process Engineering
·
2023
·
IF 7
Minjie Hou,Leiming Wang,Qinuo Ma,Tianyuan Xiao,Yanning Sun,Yanzhu Guo,Xueru Sheng,Ling-Ping Xiao,Fengshan Zhang,Pedram Fatehi,Haiqiang Shi
Pre-hydrolysis liquor (PHL) of the dissolved pulp production process contains sugar and lignin is paper-making wastewater. Although sugar has the potential for value-added applications, the presence of lignin is problematic for the utilization of PHL. To improve the removal of lignin and maintain a high concentration of sugars in PHL, a novel process was introduced that included a dilute acid treatment, activated carbon (AC), and resin treatment of PHL, respectively. The results confirmed that most of the colloidal lignin and part of dissolved lignin were removed by dilute acid post-hydrolysis and the bonds of lignin-carbohydrate complexes were broken, and the adsorption efficiency of AC and resin was improved. In addition, the research found that high temperature can improve the adsorption efficiency of lignin on AC. In the case of without using PAC, the dilute acid treatment can reduce the dosage of AC from 25 g/L to 12.5 g/L and improve the flow rate of PHL through the resin from 0.1 mL/s to 0.8 mL/s in removing lignin to 90 %, while maintaining sugars in PHL. Also, the particle size of the PHL was significantly reduced by dilute acid-post hydrolysis which is probably because of the broken of β-O-4 aryl ether bond of lignin in PHL indicated by the analysis of 2D-HSQC. This work provides a practical technical process for industrial application of PHL and production of sugar solutions (mainly xylose) with high purity.
研究领域:
生物化学工程
·
环境科学
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DOI
高影响因子
FUEL
·
2024
·
IF 6.7
Wei Tang,Xinyue Wang,Shuqi Guo,Jinxin Huan,Haojie Qian,Caoxing Huang,Yucai He
Sodium lignosulfonate (LS) is typically the primary industrial lignin derived from pulping process. Owing to LS’s surface-active properties, this work employed it in conjunction with pretreatment and enzymatic hydrolysis to biotransform poplar sawdust (PS). The findings revealed that a pretreatment of PS using 40 g/L LS and 4 g/L H 2 SO 4 (LSPS) led to the separation of 49.7 % lignin and 80.5 % xylan, consequently increasing the enzymatic hydrolysis efficiency (EHE) from 22.4 % to 66.9 %. Furthermore, an EHE of 72.8 % was achieved with 5 g/L LS, while an EHE of 71.7 % was obtained with the prehydrolysate (20 g/L LS) contained in enzymatic hydrolysis. Analysis indicated that the elevated EHE of LSPS was ascribed to enhanced accessibility and reduced surface area of lignin on one hand, and increased enzymatic activity through LS’s electrostatic adsorption on the other hand. These results underscored the high-value utilization of lignin-based surfactants in both pretreatment and enzymatic hydrolysis processes.
研究领域:
生物技术
·
可再生资源工程
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产品关联
DOI
高影响因子
Journal of Cleaner Production
·
2017
·
IF 5.715
Leichang Cao,Gang Luo,Daniel C.W. Tsang,Huihui Chen,Shicheng Zhang,Jianmin Chen
As a typical lignocellulosic biomass , abundant amount of green landscaping waste can be used as low-cost feedstock to produce value-added chemicals. This work developed a novel process to obtain industrially important cellulose acetate from green landscaping waste after separation of hemicellulose as a substrate rich in monomeric xylose . Sixteen samples of selected green waste were pretreated with diluted acid at moderate temperatures. Afterwards, the residues were treated with acetic anhydride and a small amount of sulfuric acid as catalyst to make cellulose acetate. The quantity and quality of cellulose acetate were analyzed by Fourier Transform Infrared Spectroscopy (FT-IR), Nuclear Magnetic Resonance ( 1 H NMR), and Gel Chromatography (GPC). The activation mechanisms of the diluted-phosphoric-acid pretreatment on yields and quality of cellulose acetate were also investigated using Thermogravimetric Analyzer (TGA) and Scanning Electron Microscope (SEM). Without the pretreatment, the yields of cellulose acetate from green landscaping waste ranged from 4.0% to 8.6%. After the pretreatment the mean yield of cellulose acetate from all sixteen green waste significantly increased to approximately 35%, illustrating the high efficiency and wide applicability of our proposed method.
研究领域:
生物化学
·
材料科学
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DOI
高影响因子
GREEN CHEMISTRY
·
2021
·Vol. 23
·期 7
·2738-2746
·
IF 10.182
Feiyun Li,Feiyang Qin,Yuxia Pang,Hongming Lou,Cheng Cai,Weifeng Liu,Yong Qian,Xueqing Qiu
In order to reduce the enzyme cost of lignocellulosic enzymatic hydrolysis, upper critical solution temperature (UCST) additives are used to recover and reuse cellulase by regulating the temperature. A sulfobetaine polymer, PSPE, showed a sensitive UCST response in buffer, and its UCST-performance was optimised by adjusting the molecular weight of the polymer. PSPE-3 (Mw = 355.1 kDa) was added to the cellulase buffer at 50 °C, and PSPE-3 and cellulase were co-precipitated by cooling to the room temperature of 25 °C. PSPE-3 and cellulase co-precipitated mainly through hydrophobic interactions via cooling. Compared with octadecyl sulfobetaine (SB3–18), PSPE-3 had a more sensitive UCST response and stronger cellulase recovery ability. Adding only 40% PSPE-3 (accounting for the mass fraction of cellulase) can save more than 50% cellulase in the enzymatic hydrolysis system of eucalyptus chips pre-treated with dilute acid (Eu-DA). However, for the Eu-DA system, adding 100% SB3–18 can only save about 30% of cellulase. In this work, efficient recycling of cellulase was achieved at room temperature by adding UCST-type PSPE. The process is green without consuming acid and alkali, and this work provides a new idea for reducing the production cost of cellulosic ethanol.
研究领域:
生物化学
·
可再生资源与环境工程
✓ 已验证
DOI
高影响因子
ACS Sustainable Chemistry & Engineering
·
2025
·
IF 7.3
Yequan Sheng,Xuan Wu,Jing Qi,Guijiang Wang,Cheng Dong,Xueqing Liu,Yanqing Wang,Chenhuan Lai,Xin Tan
This study presents a novel, cost-effective strategy employing nucleophilic polymers as direct additives during dilute acid (DA) pretreatment (2% H2SO4, 170–180 °C) to enhance the bioconversion efficiency of various lignocellulosic biomasses, including poplar (PP), mulberry branches (MB), and corn stover (CS). Screening among nine nucleophilic polymers identified carboxymethyl chitosan (CMS) and gelatin (GE) as exceptional dual-function enhancers to improve both prehydrolysate fermentability and enzymatic hydrolysis yield of pretreated substrates. The addition of CMS in pretreatment significantly detoxified prehydrolysates by reacting with key carbonyl inhibitors (reducing furfural by 19.8–38.6%, 5-hydroxymethylfurfural (HMF) by 3.8–59.1%, vanillin by 16.1–34.2%, syringaldehyde by 15.8–48.2% vs DA prehydrolysate controls). The addition of CMS dramatically boosted the fermentability of prehydrolysates with Saccharomyces cerevisiae, elevating ethanol yields to 7.33 (MB), 6.86 (CS), and 8.36 g/L (PP) from 6.28, 1.66, and 6.95 g/L in DA controls, respectively. Simultaneously, GE addition profoundly improved the enzyme digestibility of pretreated materials, increasing the 72-h enzymatic hydrolysis yields by 64.82 to 49.94%, 38.71 to 97.83%, and 44.26 to 55.15% for MB, CS, and PP, respectively. The findings from XPS and chemical compositional analyses indicated that the better enzyme digestibility might be related to in situ lignin modification with GE assistance. Crucially, both polymers demonstrated consistent efficacy across diverse feedstocks, with CMS also mildly aiding digestibility and GE contributing to the detoxification of pretreated biomasses. This direct addition approach with nucleophilic polymers enhanced enzyme digestibility and fermentation of pretreated materials concurrently without costly post-pretreatment steps, offering a significant advancement for efficient biofuel production.
研究领域:
生物质能源
·
生物化学工程
·
绿色化学
·
工业生物技术
·
可再生能源
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）