CELL
·
2025
·
IF 42.5
Yaozhen Chen,Shouwen Chen,Zhixin Liu,Yafen Wang,Ning An,Yutong Chen,Yihao Peng,Zheng Liu,Qin Liu,Xingbin Hu
The canonical complement-mediated lysis of mature red blood cells (RBCs) leads to severe pathogenesis. However, inhibition strategies targeting complement are not always as efficient as expected, indicating that unknown mechanisms are awaiting elucidation. In this study, we investigate the intracellular events in mature RBCs following complement activation. The collected evidence demonstrates that complement-induced hemolysis is a caspase-8-dependent programmed RBC death. Furthermore, short NLRP3 (miniNLRP3) fragments in RBCs are identified to engage in the assembly of NLRP3-apoptosis-associated speck-like protein containing a CARD (ASC)-caspase-8 complex. Activated caspase-8 directly induces the proteolysis of β-spectrin, thereby disrupting the skeletal network of the RBC membrane, a process we refer to as spectosis. Spectosis signaling is also activated in autoimmune hemolytic anemia or paroxysmal nocturnal hemoglobinuria, and the inhibition of spectosis significantly reduced complement-induced hemolysis. These findings reveal a programmed death cascade in mature RBCs, which may have important implications for the treatment of hemolytic disorders.
研究领域:
免疫学
·
血液学
·
细胞生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2015
·
IF 2.912
Jia Guo,Yuan-Yuan Bian,Ke-Xue Zhu,Xiao-Na Guo,Wei Peng,Hui-Ming Zhou
Wheat bran contains a significant amount of the anti-nutritional factor phytate. This study is the first to explore the effectiveness of activating endogenous phytase and further reducing phytate content through resulting programmed cell death (PCD). Effects of solid–liquid ratio (1:1, 1:2, 1:3, and 1:6), incubation temperature (4, 20, 38, 55, and 70 °C), metal ions (Na+, K+, Ca2+, and Mg2+), gibberellin concentration (0, 5, 50, 500, 2000, and 5000 mg/L), hydrogen peroxide concentration (0, 0.2, 0.4, 0.6, 0.8, and 1.0%), and incubation time (30, 80, 180, and 360 min) on activation of endogenous phytase activity and phytate degradation in wheat bran samples are discussed in this study. It was found that when the wheat bran was incubated with distilled water at 55 °C for 80 min, its endogenous phytase activity was dramatically increased 4-fold from 12.96 to 53.54 FTU/g, whereas the phytate content was reduced by about 70% from 45.20 to 13.52 mg/g. By comparison of photomicrographs of raw wheat bran sample and sample incubated with distilled water for 360 min at 55 °C, a conclusion could be drawn that PCD in aleurone cells had occurred.
研究领域:
食品科学
·
植物生理学
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DOI
高影响因子
Journal of Environmental Sciences
·
2025
·
IF 6.3
Jin Ma,Fang Bai,Zichao Gong,Guofei Dai,Licheng Huang,Wei Chen,Yunlu Jia,Lirong Song
A promising solution to combat harmful cyanobacterial blooms (CyanoHABs) is the use of naturally-derived cyanocidal compound. This study found that the crude extracts of the herbaceous plant Macleaya cordata can inhibit the growth of the bloom-forming cyanobacterium Microcystis aeruginosa . Growth inhibition test suggested that the inhibitory effect was significant at a concentration as low as 5 μg/L of the crude extracts. To comprehensively elucidate inhibitory mechanisms, we examined the responses in anti-oxidative systems, changes in triggering signals in cells, and hallmarks of programmed cell death (PCD) under the two levels of exposure to the crude extracts of M. cordata . A notable observation across all treatment groups was the significant increase in the superoxide dismutase activity of M. aeruginosa at 72 h. However, distinct patterns were observed in malondialdehyde (MDA), with no significant difference observed between the low concentration treatment group (5 μg/L) and the control group. In contrast, Microcystis cells subjected to high concentration (10 μg/L) exhibited a significant difference in MDA content at both 24 and 72 h. Furthermore, we observed that the levels of potential signaling molecules, e.g., intracellular reactive oxygen species, nitric oxide, and Ca 2+ were altered in the Microcystis cells after exposure to the crude extracts. This results in alterations to the levels of signaling molecules, which in turn stimulate the upregulation of orthocaspase, leading to PCD and population collapse eventually. This study examines the mechanisms of Microcystis suppression through naturally-derived substances, providing theoretical and technical foundations for the sustainable management of CyanoHABs.
研究领域:
环境生物学
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水生生态学
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植物化学
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产品关联
DOI
PubMed
高影响因子
PROTOPLASMA
·
2018
·Vol. 255
·期 4
·1039-1052
·
IF 2.457
Li Chao,Li Cheng,Wang Bingbing,Zhang Runqi,Fu Kaiyong,Gale William J.,Li Chunyan
Drought frequently occurs during wheat ( Triticum aestivum L.) grain filling. The objectives of this study were (i) to investigate the effect of post-anthesis drought on programmed cell death (PCD) in wheat endosperm cells and (ii) to examine the role of ethylene (ETH) receptors and abscisic acid (ABA) in regulating wheat endosperm PCD. Two winter wheat cultivars (‘Xindong 18’ and ‘Xindong 22’) were used in this study. Grain samples were collected from normal and drought stressed plants at 5-day intervals between 5 and 35 days post-anthesis. The samples were then compared with respect to cell viability, nuclear morphometry, cell ultrastructure, DNA integrity, nucleic acid content, and nuclease activity. Analysis was also conducted about gene transcripts related to PCD, ETH receptors, and ABA biosynthesis and degradation. Drought stress reduced cell viability, accelerated nuclear deformation, and increased mitochondrial dissolution. The activity of nucleic acid hydrolase was greater, and the nucleic acid concentrations were less in the drought treatments than in the control. As a result, the peak in DNA fragmentation occurred earlier in the drought treatment. Drought stress significantly increased the expression of four genes related to ABA ( nced1 , nced2 , ao1 , ao2 ) . In contrast, drought significantly reduced the expression of four genes related to ETH receptors ( ers1 , ers2 etr1 , etr2 ) and one gene related to PCD ( dad1 ). In summary, the results indicated that drought stress caused PCD to occur earlier in the endosperm of winter wheat.
研究领域:
植物学
·
分子生物学
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DOI
PubMed
高影响因子
Algal Research-Biomass Biofuels and Bioproducts
·
2023
·
IF 5.1
Cunhao Du,Lixiao Ni,Jakpa Wizi,Wenlu Sang,Shiyi Rong,Xianglan Li,Chu Xu,Yan Li,Xuqing Chen,Shiyin Li
Artemisinin , a natural allelochemical substance extracted from Artemisia annua , has been shown to have strong inhibitory effects on harmful cyanobacteria . However, only a few studies have systematically studied the mechanism of artemisinin in inhibiting algae and the process of algae cell death. In this study, the inhibition mechanism and death mode of M. aeruginosa under artemisinin sustained-release microspheres (ASMs) were investigated using a series of morphological, physiological and biochemical characteristics. The findings revealed that M. aeruginosa was significantly inhibited immediately after exposure to the 0.54 g·L −1 ASMs, with an inhibition rate (IR) exceeding 95 %, and the synthesis of phycobiliprotein and esterase activity were disturbed. In addition, ASMs exposure significantly increased caspase-3(-like) activities of M. aeruginosa . Under the continuous stress of ASMs, M. aeruginosa cells had three cell death modes: the first was necrosis-like cell death, which was characterized by cell wall rupture and leakage of cell contents; the second was apoptosis-like cell death, which was characterized by the disintegration of the cell structure, the integrity of the cell membrane , and the appearance of apoptotic bodies; and the last was autophagy-like cell death, which was characterized by cell swelling and appearance of autophagy vacuoles. These results indicated that ASMs could inhibit photosynthesis of M. aeruginosa cells, interfere with its antioxidant mechanisms and activate caspase-3(-like) activity, resulting in programmed cell death (PCD).
研究领域:
环境科学
·
藻类学
·
毒理学
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产品关联
DOI
高影响因子
PLANTA
·
2013
·Vol. 238
·期 4
·727-737
·
IF 3.347
Jiao Jiao,Zhou Benguo,Zhu Xiaoping,Gao Zhengliang,Liang Yuancun
Fusaric acid (FA) is a nonhost-selective toxin mainly produced by Fusarium oxysporum , the causal agent of plant wilt diseases. We demonstrate that FA can induce programmed cell death (PCD) in tobacco suspension cells and the FA-induced PCD is modulated by nitric oxide (NO) signalling. Cells undergoing cell death induced by FA treatment exhibited typical characteristics of PCD including cytoplasmic shrinkage, chromatin condensation, DNA fragmentation, membrane plasmolysis, and formation of small cytoplasmic vacuoles. In addition, caspase-3-like activity was activated upon the FA treatment. The process of FA-induced PCD was accompanied by a rapid accumulation of NO in a FA dose-dependent manner. Pre-treatment of cells with NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) or NO synthase inhibitor N
G -monomethyl-arginine monoacetate (L-NMMA) significantly reduced the rate of FA-induced cell death. Furthermore, the caspase-3-like activity and the expression of PAL and Hsr203J genes were alleviated by application of cPTIO or L-NMMA to FA-treated tobacco cells. This indicates that NO is an important factor involved in the FA-induced PCD. Our results also show that pre-treatment of tobacco cells with a caspase-3-specific inhibitor, Ac-DEVD-CHO, can reduce the rate of FA-induced cell death. These results demonstrate that the FA-induced cell death is a PCD and is modulated by NO signalling through caspase-3-like activation.
研究领域:
植物学
·
分子生物学
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DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2020
·
IF 2.751
Shuzhen Yang,Ming Fan,Dongmei Li,Jie Zhou,Gang Fan,Litao Peng,Shixin Zhang
The physiological and iTRAQ-based proteomic analyses were used to reveal the inhibitory roles of pinocembrin on mitochondria of P. italicum and its cell death mechanism. The results show that pinocembrin damages both mitochondrial structure and function. 167 and 807 differentially expressed proteins (DEPs) were detected in P. italicum mycelia after treatment with pinocembrin for 8 h and 24 h respectively, and the DEPs were significantly enriched in the oxidative phosphorylation (OXPHOS) pathway, especially for mitochondrial respiratory chain (MRC) complexes I and V. Furthermore, the expression levels of proteins related to programmed cell death (PCD) were significantly up-regulated in mycelia with Pinocembrin incubation for 24 h. Combined with the results of physio-chemical analysis, the data revealed that pinocembrin targeted MRC complexes I and V, to induce ATP depletion, enhance ROS accumulation, stimulate mitochondrial permeability transition pore (MPTP) opening, accelerate the loss of mitochondrial membrane potential (MMP) and promote cytochrome c release from mitochondria to the cytoplasm, which, as a result, effectively triggered three classical types of PCD pathways in mycelia of P. italicum .
研究领域:
真菌学
·
蛋白质组学
·
植物化学
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DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2024
·
IF 10
Jabeen Farheen,M. Zubair Iqbal,Asim Mushtaq,Yike Hou,Xiangdong Kong
Triple-negative breast cancer (TNBC) is the most common primary tumor of the breast with limited effectual drug availability. Therefore, the aim of the study is to develop an innovative phyto-nanomedicine (PNM) to cure TNBC with the least genotoxicity. Hereinafter, the sea buckthorn’ extracted polyphenols (SBP), combine with metformin (MET), are synthesized as a novel PNM to evaluate its anti-tumor properties, effectiveness, and mechanism of action in TNBC in vitro and in vivo models. The SBP exhibits 16 new kinds of polyphenols that are been reported earlier which regulated cell development, proliferation, and programmed cell death (PCD) effectively. SBP-MET PNM inhibits MDA-MB-231 (47%), MDA-MB-436 (46%), and 4T1 (46%) cell proliferation but does not affect L929 normal murine cell development and successfully induce PCD (73.19%) in MDA-MB-231 cells. Mechanistically, in vivo SBP-MET proteome expression profiling reveals upregulation of proapoptotic Bax protein and activation of Fas signaling pathways convince downstream Daxx and FADD proteins, which further triggers Caspase-3 that prompts apoptosis in human TNBC cells by cleaving PARP-1 protein. Current findings establish innovative highly biocompatible phyto-nanomedicine that has significant potential to inhibit TNBC cell growth and induce regulated cell death (RCD) in vivo model, thereby opening a new arena for TNBC therapy.
研究领域:
肿瘤学
·
药理学
·
纳米医学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Quan Ma,Dong Li,Yicheng Ren,Yanpei Chen,Jing Huang,Bin Wu,Qingqing Wang,Zisheng Luo
Autophagy (AU) and programmed cell death (PCD) are dynamically regulated during tomato fruit defense against Botrytis cinerea , which are also manipulated by pathogenic effectors to promote colonization. Present study demonstrated that the enhanced defense induced by transient inhibition on AU by hydroxychloroquine (HCQ) facilitated the restriction of B. cinerea lesion on postharvest tomato. Pre-treatment of 2 mM (16.08 ± 3.42 cm at 7 d) and 6 mM (7.80 ± 2.39 cm at 7 d) HCQ inhibited the lesion development of B. cinerea compared with Mock treatment (50.02 ± 7.69 cm at 7 d). Transient inhibition of AU induced expression of fungal defense and transcriptional regulation related genes, but attenuated reactive oxygen species (ROS) burst gene expression. The ROS-induced PCD was compromised by HCQ with promoted ROS scavenging. The transient pre-treatment of HCQ slightly inhibited AU which triggered the feedback loop that enhanced the autophagic activity defensing against B. cinerea infection.
研究领域:
植物病理学
·
分子生物学
·
农业科学
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DOI
PubMed
高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2020
·
IF 4.303
Taotao Li,Mingyang He,Jun Zeng,Zhongsuzhi Chen,Qu Hongxia,Xuewu Duan,Yueming Jiang
The effect of choline chloride (ChCl) on litchi pericarp browning and the potential regulating mechanism was investigated in this study. Results showed that 1 g L −1 ChCl significantly inhibited the development of pericarp browning and reduced respiration rate of litchi fruit. Meanwhile, choline chloride treatment delayed the programmed cell death (PCD) indicated by lower DNA laddering, inhibition of programmed cell death-related genes expression and higher soluble protein content. Additionally, ChCl treatment maintained higher ATP content and energy charge due to higher level of glycine betaine and regulation of energy metabolism related genes and ATPase activities by ChCl treatment. Finally, higher ATP status in ChCl treated fruit resulted in lower accumulation of reactive oxygen species via enhancing the activities of superoxide dismutase, catalase and ascorbate peroxidase. Taken together, the results obtained in this study suggested that choline chloride reduced pericarp browning of litchi fruit possibly through inhibiting energy deficiency mediated PCD .
研究领域:
植物生理学
·
食品科学
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DOI
高影响因子
ENVIRONMENTAL POLLUTION
·
2024
·
IF 7.6
Mengli Han,Zhao Chen,Genlou Sun,Yuxi Feng,Yuxia Guo,Shiqie Bai,Xuebing Yan
Cadmium (Cd) poses a significant threat to plant growth and the environment. Nano-Fe 3 O 4 is effective in alleviating Cd stress in plants. Elymus nutans Griseb. is an important fodder crop on the Qinghai-Tibetan Plateau (QTP). However, the potential mechanism by which nano-Fe 3 O 4 alleviates Cd stress in E. nutans is not well understood. E. nutans were subjected to single Cd, single nano-Fe 3 O 4 , and co-treatment with nano-Fe 3 O 4 and Cd, and the effects on morphology, Cd uptake, antioxidant enzyme activity, reactive oxygen species (ROS) levels and programmed cell death (PCD) were studied to clarify the regulatory mechanism of nano-Fe 3 O 4 . The results showed that Cd stress significantly decreased the germination percentage and biomass of E. nutans . The photosynthetic pigment content decreased significantly under Cd stress. Cd stress also caused oxidative stress and lipid peroxidation, accumulation of excessive ROS, resulting in PCD, but the effect of nano-Fe 3 O 4 was different. Seed germination, seedling growth, and physiological processes were analyzed to elucidate the regulatory role of nano-Fe 3 O 4 nanoparticles in promoting photosynthesis, reducing Cd accumulation, scavenging ROS, and regulating PCD, to promote seed germination and seedling growth in E. nutans . This report provides a scientific basis for improving the tolerance of Elymus to Cd stress by using nano-Fe 3 O 4 .
研究领域:
环境科学
·
植物生理学
·
农学
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
Genes & Genomics
·
2022
·Vol. 44
·期 8
·1017-1029
·
IF 2.164
Hou Fangxing,Li Xing,Wang Yanfeng,Xiao Xiangzuo
Background Liver regeneration is a highly orchestrated process concerning the modulation of various microRNAs (miRs). miR-183 was recently found to be involved in the process of liver regeneration, that miR-183 was remarkably up-regulated at 2–6 h after partial hepatectomy. Objective This study was aimed to explore the mechanism of miR-183 in on liver regeneration. Methods After partial hepatectomy (PH) or transfection, we measured the changes of miR-183 and programmed cell death protein 6 (PDCD6) levels in rats and the hepatocytes. The histopathology was observed with hematoxylin–eosin staining. The miR-183 mimic and inhibitor plasmids were intravenously injected into rats, and the liver weight/body weight ratio was calculated. The prediction of TargetScan and the validation of luciferase activity assay were employed to confirm the targeting relationship between miR-183 and PDCD6. The viability, apoptosis and cell cycle of transfected rat hepatocyte BRL-3A were determined via MTT and flow cytometry assays. Results MiR-183 expression showed a contrary tendency with that of PDCD6 during liver regeneration. Enhanced miR-183 in rats could notably increase liver/body weight ratio, while its inhibition did conversely. Overexpressed PDCD6, a target of miR-183, repressed the viability and cell cycle in hepatocytes, whereas its silence led to contrary results. Overexpressed miR-183 in BRL-3A cells enhanced cell viability and promoted the cell cycle yet suppressed apoptosis, whereas its inhibition showed contrary results, which were offset by PDCD6. Conclusions Collectively, miR-183 promoted liver regeneration via targeting PDCD6.
研究领域:
分子生物学
·
肝病学
·
细胞生物学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）