The interstitial fluid (ISF) contains rich bioinformation for disease diagnosis and healthcare monitoring. However, the efficient sampling and detection of the biomolecules in ISF is still challenging. Herein, we develop a facile but versatile ISF analysis platform by combining controllable hollow microneedles (HMNs) and elaborate microfluidic paper-based analytical devices (μPADs). The HMNs and μPADs was fixed in a bottom PDMS layer. A top PDMS layer containing a cylindrical cavity to produce negative pressure for sampling was packaged on the bottom PDMS layer. The HMNs enable efficient and swift sampling of sufficient ISF to the μPADs through one-touch finger operation without extra manipulations. The μPADs realized to simultaneously detect glucose and lactic acid in the detection area to produce chromogenic agents and analyzed by the self-programed RGB application (APP) in smartphones. The HMN microfluidic paper-based chip provides a point-of-care platform for accurate detection of biomolecules in ISF, holding great promise in the development of wearable device.
Yanhua Liu,Chaofan He,Tianhong Qiao,Guofeng Liu,Xue Li,Qianbing Wan,Zhou Zhu,Yong He
Every year, over 12 million people are enduring the persistent pain resulting from wound infections. In these chronic wounds, concealed infections may be covered up by scabs or granulation, which impeding accurate diagnosis and treatment. To address such clinical challenges, inspired by corals, a biomimetic microneedle patch (HepMi-PCL) is developed to indicate and treat infections within wound. Relying on high-precision 3D printing technology, each polycaprolactone microneedle own an equally sized porous shell and cavity with heparin-based functional hydrogel filling in. After penetrating through the scab, HepMi-PCL can absorb exudate in wound through surficial guide grooves and pores microstructure on its microneedle, thereby indicating whether anti-infection is needed. Upon the identification of a positive chronic infection, HepMi-PCL will smartly activate to release drugs rapidly. Subsequently, when the infection is effectively managed and the exudate progressively decreases, the drug delivery will correspondingly slow down or cease altogether. By leveraging autonomous response and therapeutic capabilities, HepMi-PCL demonstrates the potential to expedite the diagnosis and treatment of infections, resulting in an enhancement of healing speed for infected wounds by over 200%. Thus, the integration of diagnostic and therapeutic functions of the microneedle patch is anticipated to offer novel approaches in addressing the management of chronic wounds.
危险品化学品经营许可证（带存储）