JOURNAL OF CONTROLLED RELEASE
·
2025
·
IF 11.5
Yameng Wang,Zhiyuan Wang,Qibin Li,Yangjun Feng,Jinling Li,Yuxiang Lu,JingYing Zhang,Xue Ke
The special physiological barriers of women, such as vaginal mucus and self-cleaning behavior, pose great challenges for the treatment of vulvovaginal candidiasis ( VVC ), and the drug resistance caused by fungal biofilms limits the application of existing antifungal drugs. Based on this, we designed a “three-in-one” thermosensitive gel system (AF/BP Gel) loaded with antibiofilm nanoparticles (AF NPs) and mucus penetration-assisting nanoparticles (BP NPs) to achieve vaginal adhesion while enhancing mucus and biofilm penetration. AF NPs were loaded with farnesol (FAR) and amphotericin B (AMB), and FAR is one of quorum sensing molecules which can interfere with biofilm-related genes such as ALS3 , HWP1 , RAS1 , CPH1 , EFG1 , NRG1 , TUP1 , UME6 , and disperse mature biofilm, thus playing a synergic antibiofilm role with AMB. BP NPs was loaded with bromelain (BRO), which cleared the mucus barrier for AF NPs and help it penetrate deep into the infection. These two kinds of nanoparticles use the thermosensitive gel matrix to reach the surface of the vaginal mucosa uniformly and persistently to overcome the obstacle of vaginal self-cleaning. AF/BP Gel showed great anti- candida albicans activity in vitro and in vivo , and greatly improved the inflammatory conditions in VVC mice. Overall, this “three-in-one” thermosensitive gel system can overcome multiple physiological barriers and resist different periods of biofilm, providing a new platform for treating vagina-associated infections.
研究领域:
药剂学
·
微生物学
·
妇科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULAR PHARMACEUTICS
·
2013
·
IF 4.57
Xi Zhu,Wei Shan,Peiwen Zhang,Yun Jin,Shan Guan,Tingting Fan,Yang Yang,Zhou Zhou,Yuan Huang
Sufficient mucosal permeability is the bottleneck problem in developing an efficient intestinal delivery system of insulin. Cell-penetrating peptide-based nanocomplexes for the enhanced mucosal permeation of insulin were developed in this study. Penetratin, a cell-penetrating peptide was site-specifically modified with a bis-β-cyclodextrin group. Insulin-loaded nanocomplexes were prepared by self-assembly using penetratin or its bis-β-cyclodextrin modified derivative (P-bis-CD). A stronger intermolecular interaction and higher complex stability were observed for P-bis-CD nanocomplexes than the penetratin nanocomplexes. P-bis-CD nanocomplexes were significantly more efficient for the permeation of insulin as compared to the penetratin nanocomplexes both in vitro and in situ. Interestingly, different cellular internalization mechanisms were observed for the two nanocomplexes. In diabetic rats, intestinal administration of P-bis-CD nanocomplexes resulted in a prominent hypoglycemic effect which lasted for 6 h with maximum inhibitory rate at 60%. The relative pharmacological availability and bioavailability of P-bis-CD nanocomplexes were 10.6% and 7.1%, which were 3.0-fold and 2.3-fold higher than that of penetratin nanocomplexes, respectively. In addition, no sign of toxicity was observed after 7 consecutive days of administration of P-bis-CD nanocomplexes with endotoxin. These results demonstrated that P-bis-CD was a promising epithelium permeation enhancer for insulin and suggested that the chemical modification of cell penetration peptides was a feasible strategy to enhance their potential.
研究领域:
药学
·
纳米技术
·
药物递送系统
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
·
2025
·
IF 1.6
Li Yuan-Yuan,Yu Jia-Yan,Wang Tao,Wang Jing,Zhao Peng,Yang Xia,Wei Hong-Yuan,Chen Yue
Fab fragments obtained via enzymatic digestion of full-length antibodies or by gene recombination technology have smaller molecular weight and greater penetration ability. Those advantages make it uniquely suited to deliver radionuclides. In this study, we prepared a Fab fragment from the anti-EGFR antibody Cetuximab and labeled it with 177 Lu. The binding activity in vitro and biodistribution in vivo of [ 177 Lu]Lu-DOTA-Fab-Cetuximab were investigated. The [ 177 Lu]Lu-labeled Fab fragment showed improved tumor penetration and faster blood clearance compared to the full-length antibody.
研究领域:
医学
·
肿瘤学
·
核医学
✓ 已验证
产品关联
DOI
高影响因子
Advanced Science
·
2024
·
IF 14.3
Zhenyu Zhao,Yin Zhang,Chen Meng,Xiaoyun Xie,Wenguo Cui,Keqiang Zuo
The microvascular network plays an important role in providing nutrients to the injured tissue and exchanging various metabolites. However, how to achieve efficient penetration of the injured tissue is an important bottleneck restricting the reconstruction of microvascular network. Herein, the hydrogel precursor solution can efficiently penetrate the damaged tissue area, and ultrasound triggers the release of thrombin from liposomes in the solution to hydrolyze fibrinogen, forming a fibrin solid hydrogel network in situ with calcium ions and transglutaminase as catalysts, effectively solving the penetration impedance bottleneck of damaged tissues and ultimately significantly promoting the formation of microvascular networks within tissues. First, the fibrinogen complex solution is effectively permeated into the injured tissue. Second, ultrasound triggered the release of calcium ions and thrombin, activates transglutaminase, and hydrolyzes fibrinogen. Third, fibrin monomers are catalyzed to form fibrin hydrogels in situ in the damaged tissue area. In vitro studies have shown that the fibrinogen complex solution effectively penetrated the artificial bone tissue within 15 s after ultrasonic triggering, and formed a hydrogel after continuous triggering for 30 s. Overall, this innovative strategy effectively solved the problem of penetration resistance of ultrasound-triggered hydrogels in the injured tissues, and finally activates in situ microvascular networks regeneration.
研究领域:
生物医学工程
·
组织工程
·
再生医学
·
超声治疗
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Weiguang Wang,Shuman Zhang,Jiani Huang,Yang Xu,Qiya Gao,Zhisheng Gao,Xuxuan Gu,Huanhuan Li,Gaozhan Xie,Hui Li,Chen Xie,Runfeng Chen,Ye Tao
Near-infrared afterglow materials, which can significantly reduce background fluorescence and improve penetration in living organisms, are vital for precision imaging. However, the practicability of near-infrared afterglow materials has been hindered by their short lifetime and low luminescence quantum efficiency. Herein, we propose an effective strategy for doping water-soluble afterglow polymer host materials with fluorescent dyes, leveraging the step-by-step Förster energy transfer principle to realize near-infrared afterglow. Benefiting from the ultralong lifetime of 4.2 s and high quantum efficiency of 20.1 % for the blue host PAMCz as well as efficient cascade energy transfer efficiency of 83.3 %, the developed NIR afterglow exhibits luminescence peaked at 750 nm, a lifetime of 1.4 s, and a quantum efficiency of 17.1 %. A successful demonstration of near-infrared afterglow biological imaging with a penetration depth of 3.4 mm and signal-to-background ratio of 48.3, has been established. These advancements expand the scope of near-infrared afterglow materials by combining an ultralong lifetime with high quantum efficiency, providing a roadmap for designing robust near-infrared materials.
研究领域:
材料科学
·
生物医学工程
·
光学成像
✓ 已验证
产品关联
DOI
高影响因子
ACS Nano
·
2023
·
IF 17.1
Yiyang Guo,Yubin Ma,Xin Chen,Min Li,Xuehu Ma,Gang Cheng,Changying Xue,Yi Y. Zuo,Bingbing Sun
The penetration behavior of nanoparticles in mucous depends on physicochemical properties of the nanoparticles and the mucus microenvironment, due to particle–mucin interactions and the presence of the mucin mesh space filtration effect. To date, it is still unclear how the surface properties of nanoparticles influence their mucus penetration behaviors in various physiological and pathophysiological conditions. In this study, we have prepared a comprehensive library of amine-, carboxyl-, and PEG-modified silica nanoparticles (SNPs) with controlled surface ligand densities. Using multiple particle tracking, we have studied the mechanism responsible for the mucus penetration behaviors of these SNPs. It was found that PEG- and amine-modified SNPs exhibited pH-independent immobilization under iso-density conditions, while carboxyl-modified SNPs exhibited enhanced movement only in weakly alkaline mucus. Biophysical characterizations demonstrated that amine- and carboxyl-modified SNPs were trapped in mucus due to electrostatic interactions and hydrogen bonding with mucin. In contrast, high-density PEGylated surface formed a brush conformation that shields particle–mucin interactions. We have further investigated the surface property-dependent mucus penetration behavior using a murine airway distribution model. This study provides insights for designing efficient transmucosal nanocarriers for prevention and treatment of pulmonary diseases.
研究领域:
生物医学工程
·
药剂学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Energy Storage
·
2022
·
IF 8.907
Xintao Ma,Shuo Wang,Hui Wang,Junjie Ding,Sen Liu,Zixu Huang,Wenzhi Sun,Guijing Liu,Ling Wang,Wenlong Xu
The layered structure of layered double hydroxides (LDH) is the key to its use as an electrode material , however, the lack of efficient charge exchange between layered structures severely limits the application of LDH in supercapacitors . In this work, FeCo-LDH was composited with C 3 N 4 by hydrothermal method , and a penetrating structure was built using the layered structure of the two materials. The penetrating structure provides a channel for charge transfer between FeCo-LDH layers and also solves the self-stacking problem of C 3 N 4 . The structure of the composites was explored by X-ray diffractometer (XRD), X-ray photoelectron spectroscopy (XPS), scanning electron microscope (SEM) and transmission electron microscope (TEM), and the results showed that FeCo-LDH composited with C 3 N 4 and formed a penetrating structure. In addition, the electrochemical behavior of the electrode material was evaluated by cyclic voltammetry (CV), galvanostatic charge-discharge (GCD) and electrochemical impedance spectroscopy (EIS). Electrochemical test results show that it has a high specific capacity (593 C g −1 ) at 1 A g −1 . Furthermore, in order to study the energy storage properties of the composites, the optimal composites were designed as positive electrode as asymmetric supercapacitors , and the designed asymmetric supercapacitor still has a retention rate of 83.72 % after 10,000 cycles. This attractive performance reveals that FeCo-LDH@C 3 N 4 could as an electrode material for high-performance supercapacitors .
研究领域:
材料科学
·
电化学
✓ 已验证
DOI
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2025
·Vol. 23
·期 1
·1-22
·
IF 12.6
Jiang Luyang,Liu Jinxia,Chen Silong,Cui Wenyu,Guo Jiarui,Cheng Xiaoyu,Zheng Yingying,Yang Wenxin,Pan Zicai,Wang Yao,Zhao Mary,Han Haijie,Yao Ke,Yu Yibo
Oxidative stress, which results from the accumulation of free radicals, plays a substantial role in cataract formation. Antioxidants have shown promise in mitigating or even preventing this process. However, delivering antioxidants noninvasively to the anterior segment of the eye has been a significant challenge. In this study, we developed ceria nanoparticles modified with cyclic cell-penetrating peptides to overcome the obstruction of the dense corneal barrier on topical drug delivery. Our results demonstrated that modified ceria nanoparticles with cell-penetrating peptides (CPPs) facilitate the opening of tight junctions in human corneal epithelial cells. This characteristic considerably enhances the trans-corneal transport of nanoparticles and improves cellular uptake efficiency, while also contributing to their intracellular enrichment toward mitochondria. Further experiments confirmed that the modified ceria nanoparticles effectively counteracted ferroptosis induced by oxidative stress in lens epithelial cells both in vitro and in vivo, substantially reducing cataract formation. The successful development of ceria nanoparticles modified with cyclic cell-penetrating peptides (cCPPs) opens new avenues for research in cataract prevention and treatment. Additionally, the modified ceria nanoparticles could serve as a noninvasive drug delivery system, which holds remarkable potential for advancing drug delivery in diseases affecting the anterior segment of the eye.Graphical
研究领域:
眼科学
·
纳米医学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2019
·Vol. 7
·期 29
·4484-4498
·
IF 5.047
Fatima Zohra Dahmani,Danni Zhong,Yuchen Qi,Aboubaker El G. Dahmani,Tingting Xie,Bo Zhou,Wanli Li,Ke Yao,Lei Li,Min Zhou
Nowadays, the design of multistimuli-responsive and deep penetrating nanotherapeutics remains an auspicious means for improving the efficiency of anticancer therapeutics. Herein, we develop a targeted size-tunable nanosystem that would concurrently and uniformly release its therapeutic cargo into tumor cells by exploiting both extracellular and intracellular signals. This nanosystem (HPDAu) is composed of self-assembled pH-responsive DOX–PAMAM (PD) conjugates and ultrasmall PAMAM-stabilized gold nanoparticles (AuNPs), incorporated into a hyaluronidase-responsive hyaluronic acid nanoshell. HPDAu nanoparticles with an initial particle size of ∼100 nm could disassemble into tiny cationic nanostructures (∼5 nm, PD and AuNPs) upon incubation with HAase, and showed a burst drug-release under tumor microenvironment-mimicking conditions (HAase and acidic conditions). Such characteristics resulted in significantly improved tumor penetration both in vitro and in vivo, along with a higher cellular uptake efficiency and tumor accumulation as compared to free drug solutions. Taking advantage of these features along with the chemotherapeutic effect (DOX) and AuNP/DOX-induced radiosensitization, HPDAu plus radiotherapy (RT) treatment resulted in a substantial increase of apoptotic and cytotoxic effects against 4T1 cells. More importantly, the radio-/chemotherapeutic feasibility of HPDAu was further validated in a 4T1 orthotropic model, revealing a prominent antitumor efficacy and reduced side-toxicity compared to monotherapy and free drug solutions. Therefore, this versatile nanoplatform with active targeting, size tunability and radio-/chemotherapeutic features could be a promising tool for tumor combinatorial therapy.
研究领域:
肿瘤学
·
药剂学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2024
·
IF 5.8
Wei Zhou,Jian Yang,Xiangliang Jin
This paper reports on microcrystals with deep tissue penetration depth, strong red light emission, and thermometric function. The red light emission of NaLuF 4 :20 % Er 3+ /1 % Tm 3+ /10 % Yb 3+ microcrystals is exceptionally strong, thanks to the introduction of Tm 3+ and Yb 3+ ions energy capture centers. In bioimaging, the 1532 nm laser exhibits deeper imaging depth due to weak photon scattering and autofluorescence. Furthermore, red light emission has the greatest tissue penetration depth in the visible region. The luminescence intensity and tissue penetration depth of both the excitation and emission light make them suitable for deep tissue imaging. We investigated temperature sensing performance of NaLuF 4 :20 % Er 3+ /1 % Tm 3+ /10 % Yb 3+ microcrystals, and found that the maximum relative sensitivity of LIR (I 521 /I 540 ) reaches 0.92 %K −1 . The microcrystals NaLuF 4 :20 % Er 3+ /1 % Tm 3+ /10 % Yb 3+ are anticipated to have application in deep tissue imaging and temperature sensing.
研究领域:
材料科学
·
生物医学工程
·
光学
✓ 已验证
产品关联
DOI
高影响因子
Science Advances
·
2025
·Vol. 11
·期 6
·
IF 12.5
Yongjie Zhu,Wanying Xu,Wenwen Chen,Bowen Li,Guoyu Li,Haoran Deng,Licong Zhang,Changxuan Shao,Anshan Shan
Intracellular bacterial infections and antimicrobial resistance are threatening global public health systems. Antimicrobial peptides are a potential solution to combat bacterial resistance, but the design of self-assembled nanopeptides with dual functions of cell penetration and antibacterial properties to combat intracellular bacteria remains a challenge. Here, we propose a strategy to develop self-assembled nanopeptides with dual functions through the chimerization of self-assembled core, hydrophobic motif, and cell-permeable unit. The optimal nanopeptides, F3FT and N3FT, exhibited potent antibacterial activity and excellent biocompatibility. Crucially, F3FT and N3FT are able to efficiently penetrate cells and eliminate intracellular bacteria and sniping inflammation. Moreover, F3FT and N3FT kill bacteria mainly by disrupting bacterial cell membranes and inducing excessive accumulation of reactive oxygen species. F3FT and N3FT have exhibited good safety and potent therapeutic potential in vivo. This scheme of constructing nanopeptides through multifunctional domains design provides a paradigm for dealing with escalating of intracellular bacteria and antimicrobial resistance.
研究领域:
生物医学
·
纳米医学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2021
·
IF 6.057
Yan Yang,Rui Li,Sichun Zhang,Xinrong Zhang
A lysosome-targeting ratiometric fluorescent nanoprobe based on cell-penetrating peptides (CPPs) and quantum dots (QDs) has been developed for monitoring pH fluctuation in living cells. The as-prepared nanoprobe is constructed by Rhodamine B labeled R 9 RGD CPPs as H + response unit and the red fluorescent QDs as reference unit to achieve ratiometric pH measurement. With the help of RhB-R 9 RGD CPPs, the nanoprobe efficiently stains lysosomes and enables discernment of lysosomal pH fluctuation in cells treated with different pH buffers and drug stimulation. The method of using dye labeled CPPs to realize functionalization of nanoparticle in one-step reported herein is expected to obtain wider applications in the detection of subcellular active substances by combining different small molecular probes and functional peptides.
研究领域:
生物化学
·
细胞生物学
·
纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）