IEEE SENSORS JOURNAL
·
2024
·Vol. 24
·期 22
·36239-36246
·
IF 4.3
Dong Zhao,Honglie Chen,Kun Yang,Haoyu Wang,Xing Guo,Yang Ge,Xiushan Dong,Shengbo Sang
The application of competitive immunoassay brings many advantages to the detection of trace biomolecules and has the potential to be applied to urine-based clinical practice. However, this type of detection method has strict requirements for secondary antibody incubation processes and analysis equipment, leading to strong demands for convenient, rapid, and inexpensive detection platforms. In this study, a mechanical competitive immunosensor (MCI) was proposed for the detection of human serum albumin (HSA) based on goat anti-rabbit @Fe2O3 magnetic beads with magnetic sensitization. With the doping of Fe2O3, the conversion layer of MCI responds more accurately and rapidly to stress. In addition, goat anti-rabbit conjugated with animated Fe2O3 nanoparticles were introduced as secondary antibodies for signal amplification. Under the synergistic effect of the magnetic force of magnetic beads and the stress caused by the specific binding of antigen and antibody, the deformation of the film was amplified, which can effectively change the conductive pathway formed by doped carbon nanotubes, resulting in a larger output electric signal. Through competitive immunoassay for HSA, a limit of detection (LOD) of 68 ng/mL was achieved, which was an order of magnitude lower than direct detection methods. With high reproducibility and stability, MCI demonstrated effectiveness in the detection of HSA at a clinically significant concentration range (0.1–
$50 \; \mu $
g/mL). Moreover, MCI showed excellent specificity and selectivity, which held promise to offer an alternative tool for clinical diagnosis of urine HSA levels in nephrotic patients.
研究领域:
生物医学工程
·
分析化学
·
纳米技术
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DOI
高影响因子
Micromachines
·
2022
·Vol. 13
·期 10
·1620
·
IF 3.523
Gang Guo,Xuanye Wu,Demeng Liu,Lingni Liao,Di Zhang,Yi Zhang,Tianjiao Mao,Yuhan He,Peng Huang,Wei Wang,Lin Su,Shuhua Wang,Qi Liu,Xingfeng Ma,Nan Shi,Yimin Guan
Currently, many microchips must rely on an external force (such as syringe pump, electro-hydrodynamic pump, and peristaltic pump, etc.) to control the solution in the microchannels, which probably adds manual operating errors, affects the accuracy of fluid manipulation, and enlarges the noise of signal. In addition, the reasonable integration of micropump and microchip remain the stumbling block for the commercialization of microfluidic technique. To solve those two problems, we designed and fabricated a thermal bubble micropump based on MEMS (micro-electro-mechanical systems) technique. Many parameters (voltage, pulse time, cycle delay time, etc.) affecting the performance of this micropump were explored in this work. The experimental results showed the flow rate of solution with the assistance of a micropump reached more than 15 μL/min in the optimal condition. Finally, a method about measuring total aflatoxin in Chinese herbs was successfully developed based on the integrated platform contained competitive immunoassay and our micropump-based microfluidics. Additionally, the limit of detection in quantifying total aflatoxin (AF) was 0.0615 pg/mL in this platform. The data indicate this combined technique of biochemical assays and micropump based microchip have huge potential in automatically, rapidly, and sensitively measuring other low concentration of biochemical samples with small volume.Keywords:thermal bubble micropump;microfluidics;competitive immunoassay;aflatoxin
研究领域:
微机电系统(MEMS)
·
分析化学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Ling Zou,Yulan Tian,Xi Zhang,Jiaru Fang,Ning Hu,Ping Wang
This work describes an immunosensor for the determination of okadaic acid (OA) using a Love-wave sensor with the immunogold staining method. In this assay, OA-BSA was captured by the anti-BSA antibody (BSA-MAb) bond with staphylococcal protein A (SPA) on the sensor surface via Fc regions. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were employed to monitor the fabrication steps of immunosensor using the redox probe of K 3 Fe(CN) 6 . An indirect competitive immunoassay format was employed to detect OA after the immunosensor was prepared and gold nanoparticles (AuNPs) were introduced to amplify the phase signal. The results demonstrated that developed immunosensor was able to specifically respond to OA ranging from 10 to 150 ng/mL, providing limit of detection of 5.5 ng/mL. When applied to determine OA concentration in real sample, the results agreed well with those obtained by the commercial ELISA assay. The developed immunosensor is therefore suitable and has great application potential in detection of OA in shellfish.
研究领域:
分析化学
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食品安全
·
生物传感器
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2019
·
IF 7.65
Xuan Wang,Junhui Gong,Beilei Yuan,Zhaofang Chen,Juncheng Jiang
A multiplex suspension array detection platform of antibiotics has been developed based on silica-agarose hybrid microbeads (SAHMs). Chloramphenicol (CAP), sulfamethoxazole (SMX), metronidazole (MTZ) and amoxicillin (AMX) were employed as model analytes. The antigens (the antibiotics conjugated with BSA) were immobilized on the surface of four different types of SAHMs. Based on an indirect competition immunoassay , the selected antibiotics are detected through the competition of the specific monoclonal antibodies between the multiple antibiotics and the antigens. Due to high resistance to nonspecific protein absorption of SAHMs, the proposed method exhibited wide linear ranges (0.4˜72.9 ng/mL for CAP, 2.0˜108.5 ng/mL for SMX, 2.6˜142.2 ng/mL for MTZ, 1.0˜63.3 ng/mL for AMX) and low detection limits of 0.09˜0.8 ng/mL. Recoveries for spiked tap water samples were from 82% to 113%, with relative standard deviation lower than 14%, demonstrating the accuracy of the measurements performed with the developed method. This work offered a high-throughput, flexible and accurate tool, which provides a good platform for simultaneous detection of antibiotics.
研究领域:
分析化学
·
环境科学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Juan Li,Xu Zhao,Li-Jian Chen,Hai-Long Qian,Wen-Long Wang,Cheng Yang,Xiu-Ping Yan
Aflatoxin B1 (AFB1) contamination is one of the most critical global issues in food safety. The high carcinogenic nature necessitates rapid and specific methods for the determination of AFB1 in foodstuffs at ultratrace levels. Here, we report an enhanced bienzymatic chemiluminescence competitive immunoassay for ultrasensitive and high-throughput determination of AFB1. In this assay, protein G was first coated on the wells of a microplate for recognizing the Fc fragment of anti-AFB1 mAbs to reduce the antibody dosage and guarantee high immunological reaction efficiency. The target AFB1 competed with glucose oxidase labeled AFB1 for the limited anti-AFB1 mAbs in the wells of the microplate. p-Bromophenol was employed as an enhancer to obtain intense and long-lasting chemiluminescence. The utilization of an enhancer and bienzymatic catalysts effectively improved the detection sensitivity. The developed method offered a good linearity over 5 orders of magnitude, a detection limit of 5 pg L–1, and a relative standard deviation of 1.9% for AFB1. The application of the developed method to the analysis of grain samples gave quantitative recoveries from 94.0% to 97.0%. The developed method provides a universal platform for high-throughput, ultrasensitive, and high specific detection of pollutants or nutrients in foods.
研究领域:
分析化学
·
食品科学
·
毒理学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Yuanyuan Cheng,Xiuying Liu,Min Yang,Fei Xia,Ling Fan,Xue Gao,Xiaofei Sun,Lijie Zhu
Competitive immunochromatographic assay (ICA) has been widely used in food safety on-site monitoring. However, traditional competitive ICA relies on the change of single-signal intensity to discriminate the results, leading to limited sensitivity and accuracy of naked-eye visual inspection. In this study, we developed a sensitive ratiometric fluorescent immunochromatographic assay (RFICA) judging results based on the color changes of fluorescence. Two different color quantum dot nanobeads (QBs) were introduced as signal labels for rapid and accurate detection of 3-amino-2-oxazolidinone (AOZ) and semicarbazide (SEM) in seafoods. Under the optimal conditions, the visual detection limit for 2-NPAOZ and 2-NPSEM reached up to 1 and 0.5 ng/mL, which were 5- and 14-fold lower than that of traditional single-signal response fluorescent ICA strips. In addition, the RFICA strips were applied to real samples with acceptable recoveries of 81.33 ∼ 99.23 %. These results demonstrate that the developed RFICA platform possesses considerable potential in the on-site detection.
研究领域:
食品安全
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分析化学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2015
·
IF 3.545
Qi Wang,Yongguo Jin,Xing Fu,Meihu Ma,Zhaoxia Cai
A novel strategy for ovotransferrin (OVT) detection by tracing the “on-off-on” fluorescence signals of quantum dots (QDs) and gold nanoparticles (AuNPs) utilizing fluorescence measurements were established in this article. The immune interaction between QDs-OVT and anti-OVT-AuNPs leads to drastic quenching (turning off) of the donor by a fluorescence resonance energy transfer (FRET) process. After the addition of free OVT, anti-OVT-AuNPs peel off from the QDs-OVT surface and bind to free OVT due to competitive immunoreactions, resulting in the restoration of the fluorescence intensity of QDs (turning on). Consequently, this process can be utilized for the selective detection of OVT via optical responses. Under optimal conditions, the fluorescence intensity of the system increased linearly with increasing concentrations of OVT from 0.05 to 3.8 μg/mL. The limit of detection of OVT was 23.55 ng/mL. This method was applied to the analysis of OVT in egg powder samples. Furthermore, the strategy provided in this work could be conveniently followed to establish similar immunosensors for the rapid detection of other proteins with corresponding antibodies.
研究领域:
分析化学
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纳米技术
·
生物传感器
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Yingxu Chen,Jie Gao,Kai Yan,Xiaoling Yao,Yong Liu,Jingdong Zhang
A photocatalytic fuel cell (PFC) coupling with competitive immunoassay was designed for self-powered sensing of platelet derived growth factor-BB (PDGF-BB). In the PFC, the effective oxidation of ascorbic acid (AA) on a photoanode under visible light illumination and catalytic reduction of [Fe(CN) 6 ] 3− on a cathode generated electrical output. In order to realize the specific detection of PDGF-BB, the antibody of PDGF-BB (Ab) was immobilized on photoanode to act as the recognition element. Meanwhile, a bioconjugate, PDGF-BB@AuNPs@dopamine–melanin nanosphere, was used as the competitive element of target which could dramatically reduce the output of PFC by blocking the transfer of AA to the photoanode. When the target PDGF-BB coexisted with the bioconjugate, the competitive binding of smaller target molecule with Ab immobilized on photoanode could reduce the binding amount of bioconjugate and induce an elevated output of PFC. Under the optimum condition, the output of PFC was linearly proportional to the logarithm of PDGF-BB concentration from 20 pg mL -1 to 200 ng mL -1 , with a detection limit (3S/N) of 6.2 pg mL -1 . Moreover, the developed self-powered competitive immunosensor was successfully applied to the detection of PDGF-BB in human serum.
研究领域:
生物医学工程
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分析化学
·
纳米技术
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Juan Tang,Yapei Huang,Cengceng Zhang,Huiqiong Liu,Dianping Tang
A new impedimetric immunosensor for the fast determination of ochratoxin A (OTA) in food samples was developed based on the instant catalyst as enhancer. Initially, the signal tags were prepared via co-immobilization of anti -OTA antibody and amine-terminated dendrimer (PAMAM) on the graphene oxide nanosheets through the covalent interaction, which were utilized as a good platform for combining manganese ion ( anti -OTA-GO-PAMAM-Mn 2+ ). Upon target OTA introduction, a competitive-type immunoreaction was implemented between the analyte and the immobilized OTA-BSA on the electrode for the anti -OTA antibody on the graphene oxide nanosheets labels. After a competitive immunoassay format, the anti -OTA-GO-PAMAM-Mn 2+ were captured onto the electrode surface , which could induce the in situ formation of MnO 2 via classical redox reaction between Mn 2+ and KMnO 4 on the immunesensing platform. Moreover, the generated MnO 2 nanoparticles act as efficient catalyst could catalyze the 4-chloro-1-naphthol (4-CN) oxidation without H 2 O 2 to generate an insoluble precipitation on the platform. Under the optimal conditions, the instant catalyst based impedimetric immunosensor displayed a wide dynamic working range between 0.1 pg mL −1 and 30 ng mL −1 . The detection limit (LOD) of the assay was 0.055 pg mL −1 . The developed method exhibited high selectivity and can be used for the determination of OTA in real red wine samples.
研究领域:
食品科学
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分析化学
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生物传感器
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2014
·
IF 3.107
Jie Zhou,Kui Zhu,Fei Xu,Wenjun Wang,Haiyang Jiang,Zhanhui Wang,Shuangyang Ding
The residue of lincomycin (LIN) in edible animal foodstuffs caused by the widespread use of veterinary drugs is in need of rapid, simple, and sensitive detection methods. The present work introduces a fluorescent microsphere immunoassay (FMIA) for detecting LIN in different samples based on the competitive immunoreaction on the chromatography test strip. The residues of LIN in different samples compete with bovine serum albumin (BSA) labeled LIN conjugates on the T-line to bind to the anti-LIN monoclonal antibody labeled fluorescent microspheres (FM–mAbs). Captured FM–mAbs on the T-line represent the fluorescent intensity, which is detected under UV light and quantified by a fluorescent reader. Under optimized conditions, the dynamic range is from 1.35 to 3.57 ng/mL, and the 50% inhibition concentration (IC50) is 2.20 ng/mL. This method has 4.4% cross-reactivity with clindamycin and negligible cross-reactivity (<0.1%) with other analogues. To reduce the matrix effects, a dilution method is used to pretreat the samples, and the recoveries range from 73.92 to 120.50% with coefficient of variations <21.76%. In comparison with the results of ELISA and colloidal gold immunoassay, FMIA has obvious advantages such as easy operation, time savings, high sensitivity and specificity, and broader prospect.
研究领域:
食品科学
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分析化学
·
兽医学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 5
·1-8
·
IF 5.705
Chen Wanlu,Yao Xun,Zhou Xinchun,Zhao Kang,Deng Anping,Li Jianguo
A flower-like Au/Cu alloy nanocomposite (Au/Cu NFs) was synthesized and used in an electrochemiluminescence (ECL) based method for sensitive determination of the dye Sudan I. The Au-g-C 3 N 4 nanosheets as an ECL emitter were prepared by electrostatic adsorption between gold nanoparticles and g-C 3 N 4 . They form a film on a glassy carbon electrode (GCE) and then can be connected with Sudan I antigen via gold-nitrogen bond and amidation reactions. The Au/Cu NFs combined with Sudan I antibody also via the Au-N bond and was introduced into the modified GCE by specific recognition between the antibody and the antigen. The overlap between emission spectra of the Au-g-C 3 N 4 nanosheets and absorption spectra of Au/Cu NFs enabled the appearance of ECL resonance energy transfer process. That is, when the Sudan I analyte not present, the ECL was weakened due to absorption by the gray Au/Cu NFs on applying voltages from −1.7 V to 0 V. Conversely, the Au/Cu NFs on the GCE are reduced due to the competition for the antibody between the analyte and the antigen. A strong green ECL emission was obtained. The ECL response is linear in the 0.5 pg mL −1 to 100 ng mL −1 Sudan I concentration range, and the detection limit is 0.17 pg mL −1 .
An Au/Cu alloy flower-like nanocomposite (Au/Cu NFs) is firstly synthesized as an acceptor to constitute an electrochemiluminescence-resonance energy transfer (ECL-RET) system for sensitive measurement of Sudan I, while Au nanoparticles (Au NPs) functionalized graphitic carbon nitride (g-C 3 N 4 ) acted as a donor.
研究领域:
分析化学
·
纳米技术
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2020
·Vol. 145
·期 18
·6171-6179
·
IF 3.978
Yingqi Pei,Jing Zhang,Kang Wu,Anping Deng,Jianguo Li
A rapid and highly sensitive flow injection chemiluminescence immunoassay was developed to detect ractopamine residues in pork products. Palladium nanocubes with excellent catalytic performance for a traditional luminol–PIP–H2O2 chemiluminescence system were used as effective carriers to connect a ractopamine antibody and horseradish peroxidase (HRP). Double amplification of a chemiluminescence signal was realized because of palladium nanocubes and HRP. Carboxyl-modified resin beads were used as suitable materials to load ractopamine-coating antigens due to their good biocompatibility and large specific surface area. Based on the principle of competitive immunity, ractopamine standard solution would compete with antigen loaded on carboxyl resin beads for limited binding sites on a ractopamine antibody. Thus, the chemiluminescence intensity of an immunosensor has a linear negative correlation with the logarithm value of ractopamine concentration. Under optimal experimental conditions, the detection range of ractopamine was 0.005–1000 ng mL−1, and the limit of detection (LOD) was 1.7 pg mL−1 (S/N = 3). The proposed immunoassay possessed acceptable accuracy, high specificity and reproducibility and RAC was examined in pork and pig feed with satisfactory results, which would provide a better prospect for the detection of small molecules in food and environment analysis.
研究领域:
分析化学
·
食品科学
·
环境分析
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）