MICROPOROUS AND MESOPOROUS MATERIALS
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2021
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IF 5.455
Jianwei Bai,Shuhui Li,Xiaofei Ma,Huijun Yan,Shouzheng Su,Shuang Wang,Jun Wang
Herein, a series of novel amidoxime-functionalized porous polymeric adsorbents using dimethoxymethane (FDA) as the external cross-linker and biphenyl and amidoxime-functionalized 1-(benzyloxy)-4-ethylbenzene (BP-AO) as the original monomers were synthesized in one-pot Friedel-Crafts alkylation reactions via a simple “knitting” synthetic strategy. The adsorbents were characterized by FT-IR, solid-state 13 C NMR/MAS spectrum, N 2 adsorption-desorption, etc. The adsorption mechanism was probed by XPS , which suggests that the η 2 complexation between uranium and amidoxime, and the polymers provide an approach to synthesize novel porous materials. The batch adsorption experiments showed that amidoxime hyper-crosslinked polymer-2 (HCPA-2) displays a uranium adsorption capacity of 27.7 mg/g at pH 6. The static adsorption action was more consistent with the Langmuir model and pseudo-second-order kinetics. Ionic competition experiments showed that K d (U(IV)) is as high as 695.94 mL/g and reusable with a removal rate up to 60% after 7 cycles when 0.1 M HNO 3 was used as a stripping agent, which verifies the practical application potential of the materials.
研究领域:
材料科学
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环境科学
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产品关联
DOI
高影响因子
DYES AND PIGMENTS
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2022
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IF 5.122
Meijuan Zhang,Jian Cao,Cuiping Huang,Mingming Liu,Yanan Li,Chuanxiao Wang,Yajing Tu
Human serum albumin (HSA) accounts for 60% of total plasma protein and is closely related to life-threatening diseases. In this strategy, three fluorescent sensors of Nap-OH, Nap-C and Nap-NO 2 based on twisted intramolecular charge transfer (TICT) are designed and synthesized for detecting of HSA, and the effect of electron donating-withdrawing groups of sensors on the detection of HSA is studied. After added HSA, the fluorescent emission of Nap-NO 2 is turned on at 630 nm, and the color changed from colorless to pink. Nap-NO 2 is characterized by a 14.06-fold increase in fluorescence emission and has high sensitivity, good selectivity, fast response, and good stability. Nap-NO 2 could effectively distinguish HSA from other proteins, including bovine serum albumin (BSA). Drug competition experiments are performed that Nap-NO 2 can bind to the IB domain of HSA, avoiding the interference of commonly used drugs. Nap-OH, Nap-C and Nap-NO 2 can also be used for viscosity detection.
研究领域:
生物化学
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医学检测
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分子生物学
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
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2020
·Vol. 187
·期 12
·1-9
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IF 6.232
Su Zixian,Zhao Guangying,Dou Wenchao
Aflatoxin M1 (AFM1) residues in milk pose a major threat to human health, so there is an urgent need for a simple, rapid, and sensitive method for the determination of trace AFM1 in milk. In this study, a competitive immunochromatographic assay (ICA), using visual (PEI/PSS) 4 red silica nanoparticles (SiNPs) as signal amplification probes, was used for the highly sensitive detection of AFM1. The (PEI/PSS) 4 red SiNPs were used to label AFM1 monoclonal antibody (mAb) to prepare ICA for the detection of AFM1. After exploring the optimal conditions of mAb and immunoprobe dosage conditions, the lowest visual detection limit (VDL) of AFM1 in phosphate-buffered saline with Tween 20 (PBST, 10 mM, pH 7.4, containing 1% BSA, 3% sucrose, 1% trehalose, and 0.5% Tween 20) can reach 0.1 pg/mL. The intuitive visually visible value of AFM1 in both PBST and milk was 10 pg/mL. The results showed that the immunochromatographic system based on high chroma color (PEI/PSS) 4 red SiNPs has high sensitivity and broad application prospects for the detection of trace AFM1 residues in milk. The high chroma (PEI/PSS) 4 red SiNPs are expected to be a convenient biomarker for improving the sensitivity of immune chromatography bands.
The schematic diagram shows the detection principle. In this work, in the competitive experiment, (PEI/PSS) 4 red SiNPs were selected as visual labeling materials, and the specific antibody combined with the labeled material was selected as an immune probe. The AFM1-BSA antigen coupled with the macromolecular BSA was fixed on the T line of the nitrocellulose (NC) membrane. The AFM1 in sample solution competes with AFM1-BSA for the specific binding site of immune probe. The detection sensitivity of this method for AFM1 is obtained by judging the change of the red signal intensity produced by the positive sample, compared with the color at the T line of the negative sample.
研究领域:
食品安全
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分析化学
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纳米技术
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生物技术
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2021
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IF 7.514
Jiali Gu,Siyao Zheng,Xiyao Huang,Qian He,Ting Sun
Considering the harm of BHA on humans, thorough research of the effect of BHA on the structure of serum albumin is necessary. The binding mechanisms of BHA with bovine serum albumin (BSA) and the effects of other three food additives (butylated hydroxytoluene, benzoic acid and citric acid) on BHA-BSA system were researched by multispectroscopy and molecular docking. The fluorescence quenching experiment results showed that the fluorescence quenching mechanism of BSA by BHA was static quenching. The binding constant ((5.70 ± 0.38) × 10 3 M −1 at 298 K) and thermodynamic parameters (Δ H = 110.8 ± 2.91 kJ·mol −1 and Δ S = 443.3 ± 9.30 J·mol −1 ·K −1 ) indicated that BHA and BSA formed a relatively stable complex through hydrophobic interaction. Three-dimensional fluorescence spectra confirmed the conformation changes of BSA due to the binding of BHA. Site marker competitive experiments and molecular docking proved that BHA could bind BSA into site I in subdomain IIA. The results of molecular docking showed that BHA formed hydrophobic interactions with amino acid residues (Ala290, Leu237, Leu259, Ile263 and Ile289). The presence of other food additives weakened the binding of BHA to BSA.
研究领域:
生物化学
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分子生物学
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光谱学
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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