MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 5
·1-8
·
IF 6.232
Liu Jing,Yu Qiongqiong,Zhao Guangying,Dou Wenchao
A competitive immunochromatographic assay (ICA) is presented and used for on-site determination of ractopamine (RAC). Ultramarine blue nanoparticles were directly separated from ultramarine blue industrial products by centrifugation (< 10,000 rpm and > 4000 rpm) and used as visible labels in ICAs. The ultramarine blue nanoparticles were coated by polyacrylic acid (PAA), which provides carboxyl groups on the surface of ultramarine blue nanoparticles. An anti-RAC monoclonal antibody (mAb) was covalently immobilized on the carboxyl-modified ultramarine blue nanoparticle surface via diimide-activated conjugation between the carboxyl groups on the ultramarine blue nanoparticle surface and the amino groups of the antibodies. RAC and BSA-modified RAC competitively bind to the anti-RAC mAb on the ultramarine blue nanoparticles. The blue band in the test line is generated by the accumulation of ultramarine blue nanoparticles and is negatively associated with the RAC content. Under optimal conditions, the visual limit of detection (vLOD) of this ICA for RAC is 2.0 ng mL −1 , 2.0 ng mL −1 , and 1.0 ng mL −1 in phosphate-buffered saline (PBS), feed samples, and pork samples, respectively. The ultramarine blue nanoparticle-based ICA also shows no cross activity with salbutamol, clorprenaline, clenbuterol, or terbutaline.
Schematic representation of the ultramarine blue nanoparticles immunochromatographic assay for detection of ractopamine (RAC) based on competitive method. The ultramarine blue nanoparticles were screened from commercial ultramarine pigments for the first time and used to detect ractopamine.
研究领域:
分析化学
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食品科学
·
免疫学
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DOI
高影响因子
MOLECULES
·
2023
·Vol. 28
·期 18
·6619
·
IF 4.6
Guozheng Zhao
Based on an indirect competitive method, a novel nano-Au/fluticasone propionate electrochemical immunosensor was successfully fabricated by combining the nanoscale effect, superior conductivity of nano-Au, stable Au−S chemical bond as well as strong interaction between glucocorticoid and the receptor, which was used to simultaneously detect eight kinds of glucocorticoids. The modified immunosensors’ electrochemical properties were explored by means of a cyclic voltammetry (CV) method and electrochemical impedance spectroscopy (EIS) measurements. Two factors (glucocorticoid receptor concentration, incubation time) were studied in order to obtain the optimal results. The immunosensor presents attractive electrochemical performance with a wide linear range (between 0.1 and 1500 ng⋅mL−1) and low detection limit (between 0.057 and 0.357 ng⋅mL−1), realizing the rapid multi-residue detection of a large class of glucocorticoids. Two glucocorticoids (hydrocortisone, triamcinolone) were detected in actual skincare samples, which obtained satisfactory detection results.
研究领域:
分析化学
·
生物医学工程
·
药物分析
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Tianran Wang,Peitao Dong,Chushu Zhu,Pengxing Sha,Weiye Gao,Yulie Wu,Xuezhong Wu
Owing to the high mortality and infection rates of the Bacillus anthracis infection, commonly known as anthrax, the ability to detecting anthrax protective antigens (PA) is important in several domains, including food safety and bioterrorism. An ultrasensitive PA detection that employs a biosensor using a competitive method based on surface-enhanced Raman scattering (SERS) was developed herein. Au nanorod arrays (AuNRAs), conjugated with the PA-aptamer were prepared as substrates, whereas Au nanoparticles (AuNPs) were modified with 1,2-bis (4-pyridyl) ethylene (BPE) and PA as SERS tags. During detection, free PA and SERS tags bound competitively with active sites on the surface of AuNRAs. The Raman spectra of BPE were used to evaluate the limit of detection (LOD) for PA. Three enhanced Raman modes in this biosensor, namely AuNP self-enhancement, plasmonic resonance between single AuNP and AuNRAs, and nanogaps between two AuNP agglomerates and AuNRAs, were evaluated using the finite-difference time-domain (FDTD) method. Among AuNPs, AuNRAs and PA/BPE/AuNP/PA-apt/AuNRAs complex, the highest Raman signal of the Raman molecule BPE for the PA/BPE/AuNP/PA-apt/AuNRAs complex proved the existence of these three modes. Owing to the increased number of hotspots in biosensors, the ultra-sensitive detection of PA was achieved with an LOD of 1 pg/mL PA.
研究领域:
生物传感
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食品安全
·
生物恐怖主义检测
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2018
·
IF 2.746
Sizhu Tian,Jia Jiang,Shuang Zang,Kun Wang,Yong Yu,Xuwen Li,Hanqi Zhang,Aimin Yu,Ziwei Zhang
A novel electron spin resonance (ESR) spectroscopy method for determination of rabbit IgG was presented. Anti-rabbit IgG antibody was bound to Fe 3 O 4 nanoparticles (FeNPs). The FeNPs were used as probe to give ESR signal . After the optimization of the assay, a molar ratio 500:1 of antibody to FeNPs was selected. The sepharose beads were used as solid phase and used to separate the analyte from the sample solution. Two typical immunoassay formats, sandwich and competitive formats, were applied to the determination of IgG. The analytical sensitivities obtained by sandwich and competitive formats were 0.13 μg mL −1 and 0.56 μg mL −1 , respectively. The two formats were applied to the determination of the rabbit IgG in rabbit serum. The diluted sample was directly analyzed and other additional sample treatment was not required. The analytical results of the spiked samples indicated that the recoveries of the analytes were acceptable. Furthermore, the sample preparation time used in this method is shorter than most existing immunoassay methods .
研究领域:
分析化学
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免疫学
·
纳米技术
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产品关联
DOI
高影响因子
PHYTOCHEMICAL ANALYSIS
·
2021
·Vol. 33
·期 3
·441-451
·
IF 3.373
Xianrui Chen,Ting Huang,Zhibing Huang,Quanbin Han
Introduction Plants containing aristolochic acid and its derivatives are nephrotoxic, mutagenic, and carcinogenic to humans; chronic diet poisoning caused by the aristolochic acid is the cause of endemic (Balkan) nephropathy and related cancers. Objective To develop a colloidal gold immunochromatographic test strip (ICS) based on the competitive format for the rapid detection of aristolochic acid A (AA-A) in herbal medicinal materials. Materials and methods For the ICS based on gold nanoparticles (AuNPs), the antigen [AA-A–bovine serum albumin (BSA)], and goat anti-mouse IgG were drawn on the nitrocellulose membrane as the test line (T line) and the control line (C line), respectively. Monoclonal antibody (MAb)–AuNP conjugates were sprayed onto the conjugate pad. The sensitivity of the ICS was 6 ng/mL, and the test was completed in 10 min. The analysis of AA-A in traditional Chinese medicine samples showed that the ICS results were in good agreement with those obtained by high-performance liquid chromatography methods. Conclusion These results demonstrated that the ICS test could be used as a reliable, rapid, cost-effective, and convenient qualitative tool for on-site screening techniques to detect AA-A in herbal medicinal materials without any special instrumentation.
研究领域:
药理学
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分析化学
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毒理学
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医学
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2018
·
IF 4.244
Yuqing Zhang,Gongwei Sun,Yi Zhang,Biao Huang,Zhi Xing,Sichun Zhang,Xinrong Zhang
Inductively Coupled Plasma Mass Spectrometry (ICP-MS) based immunoassay method has been proposed in multiple immunoassays but has not been used in competitive and sandwich formats immunoassay simultaneously. The two immunoassays were usually conducted separately in clinical field depending on the size and the amount of binding sites of targets. We proposed an immunoassay method based on magnetic beads and ICP-MS detection that could be suitable for both small and large molecules. The functionalized magnetic beads were added to capture the immune complex after immune reaction. In this way, thyrotropin and free thyroid hormone can be captured, separated and then detected according to the elemental tags by ICP-MS simultaneously. The new method was evaluated by comparing the results with time resolved fluorescence immunoassays (TRFIA). The dynamic ranges of TSH and FT 4 were 0.16–105 mU/L and 3.5–65 pmol/L, respectively. The limits of detections were 0.06 mU/L for TSH and 1.59 pmol/L for FT 4 . And the relative standard deviations (RSD) of TSH and FT 4 were 4.64% at 2.5 mU/L and 1.87% at 5.85 pmol/L. This immunoassay method enables the determination of small and large biomolecules simultaneously via competitive and sandwich immunoassay formats.
研究领域:
分析化学
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临床化学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2018
·
IF 8.173
Xue Dong,Guanhui Zhao,Li Liu,Xuan Li,Qin Wei,Wei Cao
In this work, Ru(bpy) 3 2+ encapsulated in metal–organic frameworks (MOFs) UiO-67 (Ru(bpy) 3 2+ /UiO-67) as luminophor was easily prepared and firstly applied in constructing an electrochemiluminescence (ECL) immunosensor to efficiently estimate diethylstilbestrol (DES). The competitive method-based ECL immunosensor platform was fabricated by amino-silicon dioxide which possesses large surface area . The poriness of UiO-67 was splendid so that Ru(bpy) 3 2+ could be easily encapsulated. Ru(bpy) 3 2+ /UiO-67 with excellent ECL luminescence signal existed large specific surface area for easily labeled with antibodies. DES competed with bovine serum albumin-diethylstilbestrol (BSA-DES) for binding to antibody-specific sites in the constructed immunosensor. However DES was micromolecule, which was easier to bond to antibodies than BSA-DES. The ECL signal was gradually decreases with the increase of the concentration of DES. Under optimal conditions, the proposed immunosensor exhibited a wide linear range from 0.01 pg mL −1 to 50 ng mL −1 with a low detetion limit of 3.27 fg mL −1 (S/N = 3). The novel fabricated immunosensor with interference immunity and high stability may cause an attractive approach for the other targets determination.
研究领域:
分析化学
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环境科学
·
生物技术
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DOI
高影响因子
CHEMICAL PHYSICS LETTERS
·
2021
·
IF 2.328
Xiaomin Xie,Binhua Zhou,Yonglei Zhang,Guozheng Zhao,Bo Zhao
A graphene/chitosan/parathion multi-residue electrochemical biosensor was prepared by combining the formation of phosphorylated acetylcholinesterase (AChE) between organophosphorus molecules and AChE as well as the excellent conductivity of graphene, and was used to detect 11 organophosphorus pesticides (OPs) by indirect competitive method. The optical and electrochemical properties of modified electrode were studied by UV–Vis absorption spectroscopy, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The linear detection ranges for 11 organophosphorus molecules are 1–1500 ng⋅mL −1 , with detection limits of 0.012–0.23 ng⋅mL −1 , which indicates satisfactory repeatability and stability and can realize quantitative detection of organophosphorus molecules with high sensitivity. The accuracy of chlorpyrifos or dichlorvos detection in real apple samples was performed by graphene/chitosan/parathion electrochemical biosensor, which greatly meets the needs of various departments for quantitative detection of organophosphorus pesticides.
研究领域:
分析化学
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环境科学
·
农业科学
·
电化学
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产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2018
·
IF 5.123
Tao Peng,Jianyi Wang,Sijun Zhao,Yuyang Zeng,Pimiao Zheng,Demei Liang,Ghulam Mujtaba Mari,Haiyang Jiang
A multiplex lateral flow immunoassay sensor based on highly luminescent green-emitting Au nanoclusters (AuNCs-MLFIA sensor) was successfully established for the simultaneous and quantitative determination of clenbuterol (Clen) and ractopamine (RAC) in swine urine. The antigens of Clen and RAC were dispersed on a nitrocellulose membrane as two test lines, and the Au nanoclusters were synthesized from 6-aza-2-thiothymine and l -arginine to obtain highly green luminescence and ultra-small nanoparticles (Arg/ATT/AuNCs). Free carboxyl groups on Arg/ATT/AuNCs enabled conjugation with biomolecules to afford an indicator for the biosensor . The AuNCs-MLFIA sensor is based on the indirect competition assay and could successfully detect samples within 18 min without sample pretreatment , qualitative results can be obtained by visual inspection under a UV lamp. The limits of detection of Clen and RAC by the naked eye were both 0.25 μg L −1 . In addition, the AuNCs-MLFIA sensor allowed quantitative detection combined with a portable fluorescence reader. The half-maximal inhibitory concentrations of Clen and RAC were 0.06 and 0.32 μg L −1 , respectively, with detection limits of 0.003 and 0.023 μg L −1 . Thirty blind-spiked swine urine samples were analyzed by the AuNCs-MLFIA sensor and liquid chromatography–tandem mass spectrometry, and the results of the two methods showed a significant correlation. The newly developed AuNCs-MLFIA sensor overcomes several limitations of conventional LFIA sensors, including their low sensitivity, limitation to quantify analytes , and single-analyte detection.
研究领域:
分析化学
·
纳米技术
·
生物传感器
·
食品安全
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）