INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
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2022
·Vol. 57
·期 5
·2879-2885
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IF 3.612
Jie Cheng,Ning Wang,Na Li,Xiaonan Zhou,Dianyu Yu,Lianzhou Jiang
SUMMARY The high refining cost of traditional rice bran oil with high acid value and its large environmental impact have limited its further application. In this study, a lipase-catalysed hydrolysis reaction of crude rice bran oil at the oil–water interface was performed using a Pickering emulsion system stabilised by modified magnetic microcrystalline cellulose (MMC) particles. The results revealed that the modified MMC particles exhibited a rod-like structure, and a contact angle of 84.8°. In addition, the stability of the Pickering emulsion can be enhanced when the modified MMC was used as an emulsifier, and at an optimal addition amount of 2.0wt.%. The saturation magnetisation value of the emulsifier decreased from 38.8 to 8.9 emu g −1 after 12 reuses. Furthermore, the hydrolysis rates of crude rice bran oil in the Pickering emulsion system and a two-phase system at a Candida antarctica lipase B (CALB) content of 2mg mL −1 were 92.8% and 58.8%, respectively. In addition, the relative activity of CALB after 10 reuses was 71.8%. These findings provide a sustainable interface biocatalysis method and provide ideas for the rational development of high acid value oils.
研究领域:
生物化学
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食品科学
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催化化学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2022
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IF 9.231
Han Wang,Lu Chen,Shuang Wu,Weiping Jin,Wangyang Shen,Zhongze Hu,Wenjing Huang,Gang Liu
Rice bran oil bodies (RBOBs) are one of the most exploited functional components from rice bran by-products and are predominantly based on oleosin stabilization. In this study, we explored the effects of different concentrations of added (-)-epicatechin, ferulic acid, and phytic acid on the RBOBs stability. The results revealed that the incorporation of all three natural phytoconstituents could reduce the RBOBs particle size and increase emulsifying properties, demonstrating increasing surface hydrophobicity (p < 0.05), and a good antioxidant effect, which was especially obvious with (-)-epicatechin incorporation. Fourier transform infrared (FT-IR) spectroscopy data demonstrated that these three small molecule substance classes can modify with oleosin on RBOBs surface by covalent and noncovalent effects. Raman spectroscopic analysis illustrated that the vibrational modes of disulphide bonds in oleosin were modified by these three plant natural ingredients. The interactions between the three phytoconstituents and the model protein were investigated by molecular docking experiments.
研究领域:
食品科学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD PROPERTIES
·
2021
·
IF 2.727
Lipeng Han,Jiahui Li,Shujie Wang,Weiwei Cheng,Lukai Ma,Guoqin Liu,Dongxue Han,Li Niu
Glycidyl ester (GE) has attracted worldwide attention due to its potential harm to human health. The GE in edible oil forms during the deodorization process of the oil refining, where antioxidants are used to inhibit its formation. To replace unsafe conventional synthetic antioxidants with natural antioxidants, sesame oil (SO) was used to inhibit the formation of GE in corn oil (CO), palm oil (PO), and rice bran oil (RO) during the deodorization process. The results showed that the content of GE in SO, CO, PO and RO followed the decreasing order of RO (1395.88 μg/kg) > PO (376.84 μg/kg) > CO (303.24 μg/kg) > SO (133.19 μg/kg). The concentration of diacylglycerol (DAG) in SO, CO, PO and RO followed the decreasing order of RO (11.64%) > PO (7.56%) > CO (4.11%) > SO (2.59%). The content of GE in oil could be affected by at least three factors: (i) unsaturated fatty acids could promote the formation of GE in oil; (ii) antioxidants in oil could inhibit the formation of GE by scavenging free radicals; and (iii) DAG, as a precursor, could lead to the formation of GE. This result suggested that SO could inhibit the formation of GE in CO, PO and RO. This indicated that when SO was added at 50%, its inhibitory effect was equivalent to that of tert-butylhydroquinone added at 0.02%.
研究领域:
食品科学
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健康与医学
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产品关联
DOI
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 3.978
Shuting Li,Yuanyuan Deng,Junjian Peng,Guang Liu,Zhihao Zhao,Zhencheng Wei,Yan Zhang,Xiaojun Tang,Ping Li,Lihua Hou,Pengfei Zhou,Mingwei Zhang
The effectiveness of deacidification technologies for high-acid rice bran oil (HRBO) are still one of the main challenges in the industrialization process of rice bran oil (RBO). A novel function of glycerol-based deep eutectic solvents (DESs) as an acyl acceptor in combination with lipases for enzymatic esterification of deacidification of HRBO was described in this study. The maximum deacidification efficiency of 92.38% was obtained under the optimized reaction conditions. Molecular docking simulation indicated that the minimum binding energy of Novozym 435 was lower than those of Lipozyme TL IM and Lipozyme RM IM, which might be one of the underlying mechanisms for its better performance. Additionally, γ-oryzanols, tocopherols and tocotrienols were retained in deacidified RBO. We are convinced that the reusability of biocatalyst and DESs systems provide an alternative strategy to design a facile, environmentally-friendliness DESs towards economic feasibility of applications and processes for oil-refined industries.
研究领域:
食品科学与工程
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生物催化技术
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油脂化学
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产品关联
DOI
高影响因子
Food & Function
·
2021
·Vol. 12
·期 21
·10538-10549
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IF 5.396
Jiali Zhang,Lu Zhang,Zhengzhang Wu,Peng Zhang,Ruijie Liu,Ming Chang,Xingguo Wang
Phytosterols are important bioactive compounds in rice bran and rice bran oil, and the compositions of different phytosterols in rice bran and rice bran oil were investigated in our previous research. In this study, the dopaminergic neuroprotective effects and the underlying mechanisms of sitosterol, cycloartenyl ferulate, 24-methylenecycloartanyl ferulate and campesteryl ferulate identified in rice bran and rice bran oil were investigated in a rotenone-treated C. elegans model. The results indicated that the increased oxidative stress resistance induced by the activation of the DAF-16/FOXO pathway and the inhibition of the apoptotic protein CED-3 overexpression might play an important role in protecting the dopaminergic neurons. The results of this research reveal a new bioactivity of different phytosterols and are helpful for the further nutritional evaluation of rice bran and rice bran oil.
研究领域:
神经科学
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营养学
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药理学
✓ 已验证
DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2022
·
IF 9.224
Tao Yang,Yufei Zhang,Liujia Xu,Hao Zhu,Juntao Zhang,Zheng Guo,Fenghong Huang,Mingming Zheng
Herein, a pH-switchable Pickering interfacial biocatalysis (PIB) system is proposed and applied to a solvent-free biphasic biocatalysis. The oil-in-water emulsion was stabilized by amine-functionalized hollow mesoporous silica nanospheres (HMSS-N), which is functioned as emulsifiers of PIB and carriers of lipase AYS. The smart emulsifiers exhibited switch of the surface wettability behaviors in response to abrupt changes of pH. Under the optimality conditions, the immobilized lipase loading amount, activity, and expressed activity were 174.1 mg g–1, 9073 U g–1, and 52.1 U g–1 protein, respectively, at pH 6. Based on it, the hydrolysis of rice bran oil and esterification with phytosterols to produce phytosterol esters (PEs) were successfully performed. Compared with the conventional free enzyme one-phase system, the PIB system displays a higher reaction efficiency (catalytic efficiency value 6.88 mmol g–1 h–1, 20-fold enhancement). In addition, the system maintains 95% residual activity after 10 reaction cycles with 38.3 g products/g biocatalyst average productivity, complying with the requirements of industry-accepted standards. In each cycle, the demulsification is realized simply by in situ pH regulation. Therefore, this paper provides a powerful and green platform for oil–fat modification and stimuli-responsive interfacial biocatalysis with the advantages of a high efficiency, sustainability, a solvent-free process, and multi-recyclability.
研究领域:
生物技术
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化学工程
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环境科学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）