POSTHARVEST BIOLOGY AND TECHNOLOGY
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2013
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IF 2.454
Jianwen Bu,Zhendan Ni,Guzhanuer Aisikaer,Zhenhui Jiang,Zia Ullah Khan,Wangshu Mou,Tiejin Ying
Mature green cherry tomato fruit were harvested and treated with ultraviolet-C (UV-C) irradiation at a predetermined dose of 4.2 kJ m −2 , and stored at 18 °C for 35 days. The effects of UV-C treatment on color change, pigment contents, and the expression of major genes involved in carotenoid metabolism, including Psy 1, Pds , Lcy - β , and Lcy - ɛ , encoding phytoene synthase, phytoene desaturase, lycopene β-cyclase and lycopene ɛ-cyclase, respectively, were examined. The UV-C treated fruit developed a pink red color in contrast to the normal orange red color of control fruit. Lycopene accumulation during ripening in UV-C treated fruit was significantly inhibited but its final content was not affected. However, both accumulation and final content of β-carotene were significantly suppressed in UV-C treated fruit. The lower content of β-carotene, leading to a higher lycopene to β-carotene ratio, is probably responsible for the altered color phenotype in UV-C treated fruit. Psy 1, a major gene involved in lycopene synthesis was inhibited by UV-C irradiation. Significantly suppressed expression of Lcy-β gene was also observed in UV-C treated fruit. Thus it is possible that the lower transformation from lycopene to carotenes contributed to the relatively stable content of lycopene.
研究领域:
园艺学
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分子生物学
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采后科学
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产品关联
DOI
高影响因子
Frontiers in Molecular Biosciences
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2022
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IF 6.113
Pan Weimin,Zhou Yong-Ling,Wang Jian,Dai Huai-En,Wang Xiao,Liu Lin
Carotenoid metabolism is critical for diverse physiological processes. The nematode Caenorhabditis elegans has two genes that are annotated as β-carotene 15,15′-monooxygenase (BCMO) and are 17 centimorgan apart on chromosome II, but the function of BCMO-1 and BCMO-2 remains uncharacterized. Sequence homology indicates that the two enzymes belong to the carotenoid cleavage dioxygenase family that share a seven-bladed β-propeller fold with a nonheme iron center. Here we determined crystal structures of BCMO-1 and BCMO-2 at resolutions of 1.8 and 1.9 Å, respectively. Structural analysis reveals that BCMO-1 and BCMO-2 are strikingly similar to each other. We also characterized their β-carotene cleavage activity, but the results suggest that they may not act as β-carotene 15,15′-oxygenases.
研究领域:
生物化学
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结构生物学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Journal of Integrative Agriculture
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2023
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IF 4.8
Rui-xing ZHANG,Ni-nan ZHANG,Ya-xiu WANG,ABID Khan,Shuai MA,Xue BAI,Qi ZENG,Qi-ming PAN,Bao-hua LI,Lu-gang ZHANG
Carotenoids are involved in the formation of plant leaf color as well as photosystem photoprotection. This study showed that blue light significantly induced up-regulation of the total carotenoid content in the inner leaves of orange-head Chinese cabbage (OHCC). Furthermore, the transcriptomic analysis revealed that blue light treatment induced up-regulation of genes in photosynthesis ( BrHY5-2 , BrCOP1 and BrDET1 ) and the methylerythritol 4-phosphate pathways ( BrGGPS , BrDXS and BrHDR ) upstream of the carotenoid metabolic pathway. Carotenoid metabolomic analysis revealed that the accumulation of several orange and red carotenoids (lycopene, zeaxanthin, β-carotene, lutein, and β-cryptoxanthin) after blue light treatment contributed to the deepening of the leaf coloration, suggesting that short-term blue light treatment could be used to boost nutritional quality. The light signal gene BrHY5-2 participated in the blue light-induced transcriptional regulation of carotenoid biosynthesis in OHCC. Overexpression of BrHY5-2 in Arabidopsis significantly increased the total carotenoid content and the sensitivity to blue light. The above findings revealed new insights about blue-light-induced carotenoid synthesis and accumulation in OHCC lines. They suggested a new engineering approach to increase the nutritional value of vegetables.
研究领域:
植物生理学
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分子生物学
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营养科学
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产品关联
DOI
高影响因子
Aquaculture Reports
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2023
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IF 3.7
X. Tian,Y.S. Shan,N.N. Peng,X. Ma,X. Shi,H. Li,X.J. Li,X.L. Meng
The evolution of teleost coloration is strongly linked with the fish-specific genome duplication (FSGD). Sepiapterin reductase (Spr) is commonly required in pteridine pigment synthesis and two paralogs are found in teleosts. However, the functions of spra and sprb in pigmentation have not been completely elucidated. Here we found that spra was intensively expressed in skin, fins and scales of koi carp, while sprb was ubiquitously expressed in all tissues . Then, the Spr enzyme activity inhibitor, sulfapyridine (SFD), was used to explore the phenotype and genes variation after spr inhibition. Following the administration of SFD, spra was significantly suppressed but sprb was up-regulated in skin. Additionally, the color phenotypes shifted from red to yellow due to a reduction in carotenoid concentration and the number of xanthophores/erythrophores in scales. Furthermore, the genes involved in carotenoid metabolism were influenced. However, pteridine metabolism-related genes rarely exhibited obvious changes. The results indicate that spra inhibition resulted in the dysregulation of carotenoid metabolism and pigment cells reduction, whereas sprb was likely to maintain the conserved functions in pteridine synthesis for metabolic homoeostasis. This study provided evidences for the different functions of spr paralogs in carotenoid and pteridine metabolism and for better understanding of the relationship between genome duplication and color pattern diversification.
研究领域:
生物学
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遗传学
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生物化学
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DOI
高影响因子
Food Chemistry-X
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2025
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IF 8.2
Di Chen,Hailong Dong,Lvyun Zhuang,Xiaopeng Wu,Bing Wu,Weijie Lan,Miaomiao Su,Guanhua Tao,Bingqiang Xu,Chunhua Xu,Wenjiang Dong,Zhi Xu,Daizhu Lyu,Lehe Tan
Consumers are particularly fond of jackfruit for its strong tropical aroma and distinctive flavor. However, significant differences in aroma and taste exist among various jackfruit varieties. This study investigated the aroma characteristics and metabolic profiles of two representative jackfruit cultivars, QY1H and XM1H. Results revealed distinct aroma profiles between the two varieties. Lipidomics analysis indicated remarkable differences in unsaturated fatty acid metabolism, with linoleic acid metabolism emerging as a key factor contributing to aroma variation. Differences in amino acid metabolism, particularly in leucine levels, were closely associated with aroma divergence in various jackfruit types. Additionally, carotenoid metabolism varied significantly between the two cultivars, with β-cyclocitral identified as a major aroma component in the XM1H variety. These findings provide new insights into the formation mechanisms of jackfruit aroma and offer valuable data for enhancing flavor quality and guiding future breeding programs.
研究领域:
植物学
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食品科学
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代谢组学
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植物生物化学
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农业遗传学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）