Results in Chemistry
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2026
·
IF 5.8
Huanhuan Zhu,Yong Xu,Jiafen Huang,Yiheng Zhang,Manman Sun,Zhiming Wang,Yurong Wang
In this work, a sensitive glassy carbon electrode modified with palladium-doped electrochemically reduced graphene oxide (Pd@ErGO/GCE) was developed for the selective and simultaneous determination of tetrandrine (TET) and fangchinoline (FAN). The electrochemical behavior was investigated by cyclic voltammetry (CV) and a multimodal voltammetric platform was established: (1) differential pulse voltammetric (DPV) quantification of TET (LOD = 55 nM), (2) linear sweep voltametric (LSV) determination of total content (LOD = 45 nM), and (3) simultaneous determination of both analytes via DPV. The methods have great reproducibility and repeatability, great interference tolerance, and satisfactory accuracy in serum (recovery: 95.7% ~ 104%) and urine (97.4% ~ 103%). Comprehensive validation against HPLC-UV ( t = 0.32) confirmed method accuracy for pharmaceutical quality control in Radix Stephaniae tetrandrae. This methodology offers a reliable framework for alkaloid monitoring in herbal and biological matrices.
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ACS Applied Bio Materials
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2026
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IF 4.7
Yue Zhang,Xi Liu,Li Hu,Tengyun Zuo,Kun Xia,Hao Wang,Huaming Yang
The development of multifunctional hemostatic agents from earth-abundant natural silicon-based minerals represents a sustainable strategy for biomedical applications. However, a key physicochemical challenge lies in their inherent phase complexity and structural heterogeneity, which hinder predictable integration of biofunctionality. In this work, we present an inorganic–organic interfacial engineering approach to fabricate a high-performance hemostat based on phytoliths. By leveraging synergistic noncovalent interactions with functional organic molecules, such as poly(acrylic acid) (PAA), polyethylenimine (PEI), polylysine (EPL), and berberine (BER), we successfully incorporated hemostatic, antibacterial, and antioxidant properties into a unified platform. The resulting multifunctional hemostat demonstrates excellent biocompatibility, rapid blood-triggered powder-to-hydrogel transition, and outstanding water absorption capacity. In a mouse liver hemorrhage model, the engineered phytolith-based hemostat achieved a remarkably short hemostasis time of 54.83 ± 4.06 s and minimal blood loss of only 107.97 ± 5.19 mg, significantly outperforming unmodified phytoliths and rivaling a commercial zeolite-based control. This work establishes a physicochemical rationale for repurposing natural mineral resources toward advanced multifunctional hemostatic applications.
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Journal of Analysis and Testing
·
2021
·Vol. 5
·期 4
·387-395
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IF 0
Zhou Yufeng,Duan Liguang,Huang Yixin,Liu Yaqi,Wang Lijuan,Qiao Xiaoqiang
The amino acids post-modified ionic liquids-based hybrid monolithic columns were successfully developed. For the first step, using 1-vinyl-3-dodecylimidazole bromide (VDI·Br) and polyhedral oligomeric silsesquioxane methacryl substituted (POSS-MA) as monomer and crosslinking agent, the monolithic POSS-VDI matrix material was polymerized in 100 μm capillary. The prepared POSS-VDI column is of ion-exchange/reverse-phase mixed-mode retention characteristics. The column efficiency reached 137,000 N/m for thioureas. The POSS-VDI column has also been successfully applied to recognize and analyze the effective components of Traditional Chinese Medicines Fangji and the Root of Kudzu Vine. Furthermore, based on its good chromatographic performances, in the second step, amino acids l -Proline ( l -Pro), Glycine (Gly) or DL-Phenylalanine (DL-Phe) were further exploited as the anion exchangers to post-modify the POSS-VDI column. The new POSS-VDI-Pro, POSS-VDI-Gly and POSS-VDI-Phe columns exhibited different separation selectivity for the complex egg white protein sample. The present work provides a good route to fabricate versatile amino acids and ionic liquids modified monolithic columns with good separation selectivity.
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Journal of Analytical Science and Technology
·
2021
·Vol. 12
·期 1
·1-9
·
IF 2.769
Liu Mingyang,Du Xuejun,Xu Ke,Yan Binwei,Fan Zaibi,Gao Zideng,Ren Xueqin
Berberine hydrochloride (BH) is an isoquinoline alkaloid normally used as drug to treat diseases. Compared with the traditional detection methods, the carbon quantum dots (CQDs) have better selectivity, high sensitivity, easy operation, and is inexpensive which could be widely utilized as fluorescent nanoprobes to detect various compounds quantificationally. And ratiometric fluorescent sensors conspicuously increase sensitivity and precision detection and improve quantification. In this work, we use water-soluble and fluorescent cationic carbon dots cetylpyridinium chloride monohydrate (CPC)-CQDs to connect with pinacyanol chloride (PC) and sodium tetraphenylborate (ST) as the ratiometric fluorescent probe to detect BH. The ratiometric fluorescent probe has high sensitivity towards alkaloids and metal ions, photochemical stability (60 min), and pH stability (from 6.0 to 8.0), with the detection range from 0 to 200 μM, and limit was as low as 57.35 nM. The accuracy of the method was verified by spiked recovery experiment in different human blood serums which were drawn from healthy adult volunteers to explore the practicability. The recoveries were in the range 94.34 to 105.48% with relative standard deviations (RSD) of 0.80 to 2.92%. In addition, we could observe that the fluorescence was gradually darkened, and the color turned yellow to realize the visual detection. It is expected that this work would open up a new strategy for detecting BH in the environment and human blood serums.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2020
·
IF 3.99
Yan Li,Shuai Wang,Fang Xiang Song,Li Zhang,Wei Yang,Hong Xia Wang,Qian Lin Chen
Mesoporous silica nanoparticles with folic acid (MSN−COOH-Tet-HBP-FA) are able to mediate targeting and have a pH stimulation response character, and therefore have been successfully synthesized as vectors for antitumor drug delivery. The model drug is tetrandrine (Tet). The chemical structure and properties of these NPs were characterized through systematic characterization analyses. Their drug loading capacity was significantly improved through carboxy modification (26.86 % in max), and they exhibited pH-dependent drug release profiles("zero pre-release" within 20 h in a normal physiological environment). In vitro cytotoxicity and cell uptake of the as-synthesized NPs in Hela and A549 cells were also evaluated, and exhibited high cytotoxicity and cell-targeted uptake capacity. It was concluded that MSN−COOH-Tet-HBP-FA could be used as a promising drug delivery system for cancer therapy.
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Drug Design Development and Therapy
·
2021
·
IF 4.162
Wenqian Shang,Jing Zhang,Haibo Song,Shunfei Zhu,Aimin Zhang,Yushuang Hua,Shujun Han,Yan Fu
Background Endometrial cancer (EC) is one of the most common gynaecological malignancies, and its incidence has been rising over the past decade. Tetrandrine, a bisbenzylisoquinoline alkaloid, has been isolated from a vine used in traditional Chinese medicine, Stephania tetrandra . However, the key mechanism of tetrandrine in EC is still unclear. Purpose This research was designed to predict the molecular mechanisms of tetrandrine against EC based on network pharmacology and to further verify these predictions by in vitro experiments. Methods The potential therapeutic targets of tetrandrine against EC were predicted by using public databases. Afterwards, the protein–protein interaction (PPI) network of the common targets was constructed, and the key gene targets were obtained. Biological function and pathway enrichment analyses were performed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. Furthermore, molecular docking and in vitro experiments were carried out to verify the predictions. The cell counting kit‑8 (CCK‑8) assay, Hoechst 33258 staining, flow cytometry analysis, qRT-PCR, Western blot analysis and an immunofluorescence assay were performed. Results Our findings identified 111 potential therapeutic targets of tetrandrine against EC. We obtained 7 key gene targets from the PPI network analysis. Furthermore, GO enrichment analysis indicated that these targets were mainly associated with metabolic processes, responses to stimulus, and biological regulation. The KEGG pathway analysis showed that the common targets were mainly distributed in the PI3K/Akt signalling pathway. A potential interaction of tetrandrine with Akt1 was revealed by molecular docking. In addition, in vitro experiments showed that tetrandrine significantly inhibited cell proliferation and induced apoptosis in Ishikawa and HEC-1-B cells in dose- and time-dependent manners. The results also revealed that tetrandrine can downregulate the expression of Bcl-2 and upregulate the expression of Bax at the mRNA level. The mRNA levels of Akt were not significantly different in the various tetrandrine (0, 10 and 20µM) groups. However, Western blot analysis demonstrated that the protein expression ratios of p-Akt/Akt decreased at the protein level. The results were further confirmed by immunofluorescence assays. Conclusion Based on bioinformatic analysis and experimental verification, our findings demonstrated that tetrandrine exerted tumour-suppressive effects on EC by regulating the PI3K/Akt signalling pathway.
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JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
·
2014
·
IF 0.638
Zhisheng Xie,Xinjun Xu,Chunyan Xie,Zhikun Liang,Mei Yang,Jieyun Huang,Depo Yang
Tetrandrine and fangchinoline were successfully separated by reversed-phase flash chromatography on a manually-packed C18 column from Radix Stephania tetrandra. After recrystallization by acetone, 13 mg of fangchinoline and 21 mg of tetrandrine were obtained from 100 mg Stephania tetrandra extract, with their relative content 98.78% and 98.19%, respectively. Their structures were characterized by UV, IR, MS, and NMR. The established method was simple, fast, and reproducible, which can be applied to the preparation of reference substances of tetrandrine and fangchinoline.
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JOURNAL OF CHROMATOGRAPHY A
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2015
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IF 4.169
Qing Wang,Zhi-Yuan Luo,Mao Ye,Yu-Zhuo Wang,Li Xu,Zhi-guo Shi,Lanying Xu
The zirconia-coated silica (ZrO 2 /SiO 2 ) material was obtained by coupling layer-by-layer (LbL) self-assembly method and sol–gel technology, to take dual advantages of the suitable porous structure of SiO 2 and basic resistance of ZrO 2 . Adenosine 5′-monophosphate (5′-AMP) was then self-assembled onto ZrO 2 /SiO 2 via Lewis acid–base interaction, generating 5′-AMP-ZrO 2 /SiO 2 . The chromatographic properties of 5′-AMP-ZrO 2 /SiO 2 were systemically studied by evaluating the effect of acetonitrile content, pH and buffer concentration in the mobile phase. The results demonstrated that the 5′-AMP-ZrO 2 /SiO 2 possessed hydrophilic interaction chromatographic (HILIC) property comprising hydrophilic, hydrogen-bonding, electrostatic and ion-exchange interactions. For basic analytes, the column efficiency of ZrO 2 /SiO 2 and 5′-AMP-ZrO 2 /SiO 2 was superior to the bare ZrO 2 , and different selectivity was obtained after the introduction of 5′-AMP. For acidic analytes, good resolution was obtained on 5′-AMP-ZrO 2 /SiO 2 while the analysis failed on the bare ZrO 2 column owing to strong adsorption. Hence, the proposed 5′-AMP-ZrO 2 /SiO 2 had great potential in analyzing acidic compounds in HILIC mode. It was an extended application of ZrO 2 based SP.
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高影响因子
NEUROCHEMICAL RESEARCH
·
2013
·Vol. 38
·期 9
·1871-1879
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IF 2.125
Ruan Lin,Huang Huan-Sen,Jin Wen-Xiang,Chen Hai-Ming,Li Xiong-Juan,Gong Qing-Juan
Ischemic stroke is the most common type of stroke and brings about a big disease burden because of high mortality and disability in China. Tetrandrine, a bisbenzylisoquinoline alkaloid isolated from the Chinese herb Radix Stephania tetrandra, has been demonstrated to possess anti-inflammatory and free radical scavenging effects and even regulate astrocyte activation, but the possible role of tetrandrine in ameliorating cerebral ischemia/reperfusion injury of ischemic stroke remains unknown. The aim of this study was to determine the effects of tetrandrine on neurological injury and differential proteomic changes induced by transient reversible middle cerebral artery occlusion (MCAO) in mice. Male Balb/c mice were divided into sham (n = 30), MCAO + saline as control (n = 30), and MCAO + Tet as tetrandrine-treated (n = 30) groups. Mice in the control and tetrandrine-treated groups underwent 120 min of MCAO following reperfusion. Immediately and 2 h after MCAO, the mice received either normal saline (sham operated and control groups) or tetrandrine (tetrandrine-treated group) intraperitoneally. Neurological defects, brain water content, and infarct volume at 24 h after stoke were used to evaluate neurological injury extent. Treatment with tetrandrine not only mitigated cerebral neurological deficits ( P < 0.05) and infarct size ( P < 0.01), but also decreased brian edema in the ischemic brain ( P < 0.05). Then, fluorescence two-dimensional difference in gel electrophoresis was used to detect our systematic differential profiling of proteomic changes responding to tetrandrine administration. We validated that the expression of GRP78, DJ-1 and HYOU1 was associated with neuroprotective effect of tetrandrine in MCAO model by Western blotting. These findings indicate a potential neuroprotective role of tetrandrine for ischemic stroke and yield insights into cellular and molecular mechanisms of tetrandrine taking place in ischemic stroke.
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TALANTA
·
2015
·
IF 3.545
Qing Wang,Yao Long,Lin Yao,Li Xu,Zhi-Guo Shi,Lanying Xu
A mixed-mode chromatographic stationary phase , C18-DTT (dithiothreitol) silica (SiO 2 ) was prepared through “thiol-ene” click chemistry. The obtained material was characterized by fourier transform infrared spectroscope, nitrogen adsorption analysis and contact angle analysis. Chromatographic performance of the C18-DTT was systemically evaluated by studying the effect of acetonitrile content, pH, buffer concentration of the mobile phase and column temperature. It was demonstrated that the novel stationary phase possessed reversed phase liquid chromatography (RPLC)/hydrophilic interaction liquid chromatography (HILIC) mixed-mode property. The stop-flow test revealed that C18-DTT exhibited excellent compatibility with 100% aqueous mobile phase. Additionally, the stability and column-to-column reproducibility of the C18-DTT material were satisfactory, with relative standard deviations of retention factor of the tested analytes (verapamil, fenbufen , guanine , tetrandrine and nicotinic acid) in the range of 1.82–3.72% and 0.85–1.93%, respectively. Finally, the application of C18-DTT column was demonstrated in the separation of non-steroidal anti-inflammatory drugs, aromatic carboxylic acids , alkaloids, nucleo-analytes and polycyclic aromatic hydrocarbons . It had great resolving power in the analysis of various compounds in HILIC and RPLC chromatographic conditions and was a promising RPLC/HILIC mixed-mode stationary phase.
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2016
·
IF 3.169
Qing Wang,Ling Tong,Lin Yao,Peng Zhang,Li Xu
In the present study, a mixed-mode stationary phase, C18-Diol, was applied for fingerprint analysis of traditional Chinese medicines. Hydrophobic, hydrogen bonding and electrostatic interactions were demonstrated to contribute the retention separately or jointly, which endowed the C18-Diol stationary phase with distinct selectivity compared to the bare C18 one. The separation of total alkaloids extracted from Fritillaria hupehensis was compared on the C18-Diol and conventional C18 column with the greater resolving power and better symmetry responses on the former one. Besides, a novel two-dimensional liquid chromatography on the single column (2D-LC-1C) was realized on C18-Diol with the offline mode for the alcohol extract of Fritillaria hupehensis and online mode for Ligusticum chuanxiong Hort. The early co-eluted extracted components with great polarity on the first dimension were reinjected on the same column and well separated on the second dimension. The results exhibited that the two complementary RPLC and HILIC modes on C18-Diol stationary phase enhanced the separation capacity and revealed more abundant chemical information of the sample, which was a powerful tool in analyzing complex herbal medicines.
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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2011
·
IF 2.733
Lihong Liu,Shenglan Li,Zilin Chen
A simple and selective method was developed for the simultaneous determination of tetrandrine and fangchinoline in herbal medicine by HPLC with electrochemical detection (ECD) on a bare glassy carbon electrode. The HPLC separation and ECD conditions have been optimized. The separation was carried out on a WondaSil ® C18-WR column (4.6 mm × 250 mm, 5 μm), with the mobile phase of acetonitrile–ammonium acetate buffer (pH 6.5; 40 mM) (32:68, v/v) using an isocratic elution at the flow rate of 0.5 mL/min. The electrochemical detection potential was set at +0.9 V. The obtained LODs for tetrandrine and fangchinoline were 0.26 and 0.27 μmol/L, respectively. The method was successfully applied to the analysis of tetrandrine and fangchinoline contents in Stephania tetrandra S. Moore. It has been demonstrated that the LC-ECD method is an excellent technique for analysis of the herbal medicine. The mean recoveries were in the range of 95–105%, while the precision expressed as repetition of peak area was lower than 2.7%.
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