Chinese Journal of Natural Medicines
·
2017
·
IF 1.667
Xiao-Xiao LU,Yi-Fan JIANG,Hong LI,Ying-Ye OU,Zhi-De ZHANG,Hong-Ye DI,Dao-Feng CHEN,Yun-Yi ZHANG
Lipopolysaccharides (LPS) contamination in herbal crude polysaccharides is inevitable. The present study was performed to explore the effect of polymyxin B on abolishing the influence of LPS contamination in mononuclear cells. LPS was pretreated with polymyxin B sulfate (PB) at different concentrations for 1, 5 or 24 h, and then used to stimulate RAW264.7 and mouse peritoneal macrophages (MPMs). The nitric oxide (NO) and tumor necrosis factor-α (TNF-α) in cell culture supernatant, as the indications of cell response, were assayed. Bupleurum chinensis polysaccharides (BCPs) with trace amount contamination of LPS was treated with PB. 30 μg·mL −1 of PB, treating LPS (10 and 1 000 ng·mL −1 in stimulating RAW264.7 and MPMs respectively) at 37 °C for 24 h, successfully abolished the stimulating effect of LPS on the cells. When the cells were stimulated with LPS, BCPs further promoted NO production. However, pretreated with PB, BCPs showed a suppression of NO production in MPMs and no change in RAW264.7. In the in vitro experiments, LPS contamination in polysaccharide might bring a great interference in assessing the activity of drug. Pretreatment with PB (30 μg·mL −1 ) at 37 °C for 24 h was sufficient to abolish the effects of LPS contamination (10 and 1 000 ng·mL −1 ).
研究领域:
免疫学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2023
·Vol. 12
·期 8
·1590
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IF 5.2
Yaxian Chen,Yini Cai,Zhimin Zhao,Depo Yang,Xinjun Xu
In this study, the hot water extraction process of crude polysaccharides from Morindae officinalis radix (cMORP) was conducted and optimized through a single-factor test and orthogonal experimental design. With the optimal extraction process (extraction temperature of 80 °C, extraction time of 2 h, liquid/solid ratio of 15 mL/g, and number of extraction of 1), the cMORP was obtained by the ethanol precipitation method. The chemical properties and preliminary characterization of the cMORP were analyzed by chemical or instrumental methods. Furthermore, to indicate a preliminary study on safety, a single oral dose of 5000 mg/kg body weight (BW) was administered orally to Kunming (KM) mice for acute toxicity, and the cMORP was administered orally to KM mice once a day at doses of 25, 50, and 100 mg/kg BW for 30 days. General behaviors, body weight variations, histopathology, relative organ weights, and hematological and serum biochemical parameters were observed and recorded. The results suggested there were no toxicologically significant changes. Based on the safety study, cMORP can be initially considered non-toxic with no acute oral toxicity up to 5000 mg/kg BW and safe at up to 100 mg/kg BW in KM mice for 30 days.Keywords:Morindae officinalis radix;crude polysaccharides;hot water extraction;optimization;preliminary characterization;safety study
研究领域:
药用植物学
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药学
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毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Royal Society Open Science
·
2019
·Vol. 6
·期 7
·
IF 2.515
Mingxu Chu,Mingtang Li,Zhaolian Han,Jinshan Cao,Rui Li,Zhiqiang Cheng
In this present work, smoke-like carbon was successfully fabricated from a bio-waste fungal substrate crude polysaccharide for the first time. The as-prepared products possess smoke-like structures, ultra-high specific surface area (SBET: 2160 m2 g−1) and a high content of micropores (microporous surface area of 60%, with a nanopore size of 0.70 nm), which can increase the specific capacitance, representing a wonderful structure for electrochemical energy storage devices. The as-prepared sample displayed an excellent specific capacitance of 152 F g−1 at 5 A g−1 in the three-electrode configuration and exhibited maximal densities of 6.8–10.2 W h kg−1 under power outputs of 253.4–24.3 kW kg−1. We believe that this work demonstrates a simple, green and low-cost route by using agricultural residue to prepare applicable carbon materials for use in energy storage devices.
研究领域:
材料科学
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能源存储
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2019
·
IF 6.97
Wenmei Ao,Juan Han,Lei Wang,Chunmei Li,Yanli Mao,Yun Wang
A novel fractional purification approach of crude polysaccharides with a boronic acid-tagged thermoresponsive triblock copolymer PEG113-b-PVBA49-b-PNIPAM105 was designed in this paper. The fractional precipitation of polysaccharides was realized through simple temperature regulation based on the difference in the lower critical solution temperatures of the triblock copolymer–dextran complex. The precipitated polysaccharides from the extracting solution was in a descending order of molecular weight. Under optimal conditions, four astragalus polysaccharide fractions (APS1, APS2, APS3, and APS4) with narrow distribution of molecular weights were obtained with the temperature-controlled fractional precipitation. The structural properties, monosaccharide compositions, and molecular weights of APS1, APS2, APS3, and APS4 were characterized by the Fourier transform infrared spectrum, Congo red spectrophotometric method, gas chromatography, and gel permeation chromatography. The differences in the biological activities of various APS indicate that the molecular weights of polysaccharides have an influence on the activities of APS. This novel temperature-controlled fractional precipitation method has a favorable prospect in the fractional purification of polysaccharides.
研究领域:
生物化学
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材料科学
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药学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）