BIOMASS & BIOENERGY
·
2023
·
IF 6
Wen-jie Cong,Jian Yang,Jianguo Zhang,Zhen Fang,Zheng-diao Miao
Magnetic self-assembly metal-organic frameworks (MOFs) were constructed for the co-production of biodiesel and hydrogen from waste oils. The highly active MOF nanoparticles were synthesized and catalyzed crude glycerol with 126.8% hydrogen yield in subcritical water (350 °C, 5 min). By coupling alkali with MOF-derived carriers, continuous process of fast production of biodiesel (with microwave heating at 90 °C in 15 min, 15:1 methanol/oil molar ratio and 9 wt.% catalyst dosage) and hydrogen (350 °C, 5 min) were achieved with yield of 95.3% biodiesel from waste oil (AV of 3.95 mg KOH) and 102.6% H 2 from crude glycerol by-product, respectively. The nanoparticles were magnetically separated for 5 cycles with 95% biodiesel yield. Subsequently, the deactivated catalyst was used for hydrothermal gasification with 50% increasing in hydrogen yield. Characterization techniques showed active sites of MOF-derived nanoparticles were well dispersed with surface area increased by 3.9 times for highly efficient production of biodiesel and hydrogen. It revealed that MOF materials can be designed to make active catalysts and carriers for loading catalytic sites for biomass conversions to targeted biofuels.
研究领域:
能源与环境科学
·
催化化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
·
2016
·Vol. 92
·期 7
·1801-1808
·
IF 2.738
Qinli Liu,Ting Yan,Zhao Jiang,Tao Fang
BACKGROUND The biodiesel by-product, crude glycerol including lower alcohols, could be used as the feedstock for producing monoacyglycerols (MAGs), which are commonly used as non-ionic surfactants and emulsifiers. In this work, lower alcohols (methanol, ethanol and isopropanol) in sub- and supercritical states were used as the solvents for MAG production. RESULTS Isopropanol is the best solvent for obtaining high monooleate (MOG) yield compared with methanol and ethanol. Moreover, the sub- and super-critical isopropanol shows better performance than the normal NaOH catalyzed or non-solvent process. The reaction loading of a batch reactor has a complicated effect on the yield. The maximum MOG yield can always be obtained at 60% loading. The square root modified quadratic model was utilized to analyze the relationship and to optimize reaction temperature (220–260 °C), reaction time (30–150 min), and isopropanol (0–4 mL). In the optimum condition (260 °C, 150 min, 4 mL of isopropanol), a high MOG yield of 67.8% can be obtained, well in agreement with the predicted value. CONCLUSION Isopropanol at sub- and super-critical state greatly improved the mass transfer between the immiscible reactants (triglyceride and glycerol). Compared with some methods with chemical catalyst, the method without any catalyst obtained higher MOG yield. © 2016 Society of Chemical Industry
研究领域:
生物化学
·
可再生能源
✓ 已验证
产品关联
DOI
高影响因子
GREEN CHEMISTRY
·
2024
·
IF 9.3
Daocheng Liao,Shiming Tang,Ying Lin,Suiping Zheng
1,3-Propanediamine is a diamine widely used in microelectronics and porous materials. It can also be used as a curing agent for epoxy resin, forming valuable three-dimensional structures, or as a raw material for synthesizing the radiation-resistant drug amifostine. However, traditional chemical synthesis methods for producing 1,3-propanediamine often contradict the 12 principles of green chemistry. In this study, we designed a new pathway for producing 1,3-diaminopropane through a retrosynthetic-designed multi-enzyme cascade from crude glycerol in vitro. Compared with traditional chemical methods, this multienzyme cascade is simpler in process, less energy-consuming, cleaner in substrate usage, and more environmentally friendly. In the cascade reaction, we selected glycerol dehydratase (KpGDHT) to dehydrate the intermediate product 3-amino-1,2-propanediol based on substrate promiscuity. We then modified the substrate preference of KpGDHT to obtain the optimized mutant KpGDHT/Q337A/S302D, which exhibited a 4.4-fold increase in activity toward 3-amino-1,2-propanediol compared with the wild-type KpGDHT. Using KpGDHT/Q337A/S302D as the catalyst, 3.8 mM 1,3-diaminopropane was produced from 5 mM glycerol within 8h under optimal conditions, achieving 76% conversion efficiency. Finally, we evaluated the performance of 1,3-diaminopropane synthesized from crude glycerol and obtained 1,3-diaminopropane with a concentration of 3.95 mM, which is comparable to that from pure glycerol. Given its robustness and environmental benefits, this proposed cascade reaction pathway has great potential to produce 1,3-diaminopropane on an industrial scale.
研究领域:
生物化学
·
合成生物学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
CHINESE JOURNAL OF CHEMICAL ENGINEERING
·
2018
·
IF 1.712
Ying Qing,Houfang Lu,Yingying Liu,Changjun Liu,Bin Liang,Wei Jiang
The biodiesel production technology catalyzed by 1,8-diazabicycloundec-7-ene (DBU) is developed in this work. Crude glycerol containing DBU and DBU/glycerol/CO 2 (DGC) ionic compounds reacts directly with dimethyl carbonate (DMC) to produce high value-added glycerol carbonate (GC) catalyzed by DBU and DGC. The catalytic performance of DBU and DGC, as well as the kinetics of the reaction catalyzed by DBU, were investigated. The results show that DGC has a weak catalytic effect on the transesterification of glycerol and DMC. When the temperature is higher than 60 °C, DGC catalyzes the reaction jointly with DBU, which is produced from the decomposition of DGC. DBU has a good catalytic effect on the reaction between glycerol and DMC, with 90% conversion of glycerol and 84% selectivity to GC under the following conditions: DMC-to-glycerol molar ratio of 3:1, 4.0% DBU (based on glycerol mass), reaction time of 60 min, and reaction temperature of 40 °C. The apparent kinetics results show that the activation energies are 30.95 kJ·mol − 1 and 55.16 kJ·mol − 1 for the forward and reverse GC generation reactions, respectively, and the activation energy of the decomposition reaction of GC to glycidol (GD) is 26.58 kJ·mol − 1 .
研究领域:
化学工程
·
催化科学
✓ 已验证
产品关联
DOI
高影响因子
ACS Synthetic Biology
·
2023
·
IF 4.7
Hui-Hui Li,Jie Wu,Jia-Qi Liu,Qi-Zhong Wu,Ru−Li He,Zhou-Hua Cheng,Jun-Lu Lv,Wei-Qiang Lin,Jing Wu,Dong-Feng Liu,Wen-Wei Li
Polyhydroxybutyrate (PHB) is an attractive biodegradable polymer that can be produced through the microbial fermentation of organic wastes or wastewater. However, its mass production has been restricted by the poor utilization of organic wastes due to the presence of inhibitory substances, slow microbial growth, and high energy input required for feedstock sterilization. Here, Vibrio natriegens, a fast-growing bacterium with a broad substrate spectrum and high tolerance to salt and toxic substances, was genetically engineered to enable efficient PHB production from nonsterilized fermentation of organic wastes. The key genes encoding the PHB biosynthesis pathway of V. natriegens were identified through base editing and overexpressed. The metabolically engineered strain showed 166-fold higher PHB content (34.95 wt %) than the wide type when using glycerol as a substrate. Enhanced PHB production was also achieved when other sugars were used as feedstock. Importantly, it outperformed the engineered Escherichia coli MG1655 in PHB productivity (0.053 g/L/h) and tolerance to toxic substances in crude glycerol, without obvious activity decline under nonsterilized fermentation conditions. Our work demonstrates the great potential of engineered V. natriegens for low-cost PHB bioproduction and lays a foundation for exploiting this strain as a next-generation model chassis microorganism in synthetic biology.
研究领域:
生物技术
·
微生物学
·
合成生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Waste and Biomass Valorization
·
2024
·Vol. 15
·期 6
·3519-3533
·
IF 2.6
Fu Kai,Zhang Linda,Lin Yucheng,Zhang Weihong,Zhao Zigao,Chen Wei,Chang Chun
Currently, polyurethane (PU) products are heavily dependent on petroleum resources, highlighting an urgent need to develop new bio-based materials. This study aimed to develop a sustainable method of utilizing low-cost and renewable xylose residues (XR) and crude glycerol (CG) for the production of biopolyols. Optimal synthesis conditions were determined to be 220 °C, 3 h, and 7% sodium hydroxide loading, resulting in biopolyols with a hydroxyl number of 505 mg KOH/g and an acid number of 1.7 mg KOH/g. The obtained biopolyols were used to produce biobased PU foams with a compressive strength of 225 kPa, density of 0.048 g cm −3 , and thermal conductivity of 0.0355 W m −1 K −1 . Characterization analysis using FT-IR, 1 H NMR, and TGA confirmed the excellent thermal stability and insulation properties of the bio-based PU foam. This study provides a valuable method for producing sustainable bio-based PU foam from XR and CG, thereby presenting a novel approach to realizing the high-value utilization of both resources. Graphical Abstract
研究领域:
生物化学
·
材料科学
·
可再生资源利用
✓ 已验证
DOI
高影响因子
ENVIRONMENTAL POLLUTION
·
2020
·
IF 6.793
Xiang Wang,Si-Fen Liu,Zi-Hao Qin,Srinivasan Balamurugan,Hong-Ye Li,Carol Sze Ki Lin
Waste streams have emerged as potential feedstocks for biofuel production via microbial bioconversion . Metabolic engineering of the microalga Phaeodactylum tricornutum in its lipid biosynthetic pathways has been conducted with an aim to improve lipid production. However, there has been only limited achievement in satisfying biofuel demands by utilising extracellular organic carbons from low-cost waste streams. Herein, we present a successive staged cultivation mode, based on a previously engineered strain that co-overexpresses two key triacylglycerol biosynthesis genes. We first optimised microalgal biomass and lipid production by using food waste hydrolysate and crude glycerol as the cultivation media. Food waste hydrolysate (5% v / v ) is a low-cost organic carbon source for enhanced microalgal biomass production, and the resulting lipid concentration was 1.08-fold higher with food-waste hydrolysate than that of the defined medium. Additionally, the resultant lipid concentration after using crude glycerol (100 mM) was 1.24-fold higher than that using the defined medium. Two carbon feeding modes (hybrid and sequential) were also performed to investigate the potential of engineered P. tricornutum with preliminary mechanistic analyses. The biodiesel properties of lipids produced in the hybrid mode were evaluated for potential application prospects. Collectively, this study demonstrates a waste stream utilisation strategy for efficient and sustainable microalgal biofuel production.
研究领域:
生物技术
·
生物能源
·
环境工程
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2019
·
IF 6.669
Xiang Wang,Srinivasan Balamurugan,Si-Fen Liu,Man-Man Zhang,Wei-Dong Yang,Jie-Sheng Liu,Hong-Ye Li,Carol Sze Ki Lin
This study investigates the prospective of utilizing kitchen wastewater and food wastes, biofuels industry byproducts as alternative water and carbon sources. Kitchen wastewater did not impede cellular growth rate of the evolved Phaeodactylum strain E70, which indicates its potential as an alternative to freshwater resources. Among the organic wastes assessed, food waste hydrolysate significantly increased cell growth. Supplement of crude glycerol in cultivation medium enhances the total fatty acid content. Mixed food waste hydrolysate and crude glycerol remarkably increased both the cell density and total fatty acid content. Also, the supplement of butylated hydroxytoluene alleviated the oxidative stress induced by impurities in organic wastes and concomitantly increased microalgal total fatty acids and polyunsaturated fatty acids content. The experimental results reported in this study show that a waste-based biorefinery could lead to utilization of organic waste resources for the efficient production of value-added products.
研究领域:
海洋生物学
·
生物技术
·
环境科学
·
生物化学工程
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）