PEST MANAGEMENT SCIENCE
·
2021
·Vol. 78
·期 1
·43-51
·
IF 4.845
Xiaojing Yan,Huizhu Yuan,Yixuan Chen,Xin Shi,Xiaohui Liu,Zhenying Wang,Yue Liu,Daibin Yang
BACKGROUND The fall armyworm (FAW), Spodoptera frugiperda (J. E. Smith), is an invasive pest. Maize is one of its preferred host plants. The traditional application of granules has been effective for the control of FAW. Nevertheless, the challenge is to develop an application method that is less labor-intensive and also efficient. RESULTS Tiny granules in the size range of 0.38–0.55 mm were prepared by the centrifugal granulation method for drone application. The in-swath distribution patterns of granules deposited on sticky tapes were similar to the distribution of aqueous droplets through drone spraying. In the range of 1.5–3.5 m, a relatively higher aircraft height of flight can provide a more uniform distribution pattern of deposited granules, but resulted in less deposit of tiny granules in the whorl of maize plants. Similarly, the deposit of granules in the whorl of maize plants significantly decreased when the flight speed increased from 4 to 6 m s −1 . Granules gathered in the whorl accounted for 50.8–58.7% of total granules deposited in the maize canopy. The field experiment demonstrates granular formulations (containing 0.25% chlorantraniliprole + 0.15% emamectin benzoate, or higher concentrations) can provide better and longer duration of FAW control than the aqueous spray formulation at the whorl stage of maize plants. CONCLUSION Broadcasting of tiny granules by drone can provide better FAW control than liquid spray. The architecture of the maize plant and the downward airstream of the drone create favorable conditions for the deposition of granules in whorls. © 2021 Society of Chemical Industry.
研究领域:
农业昆虫学
·
害虫防治
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DOI
高影响因子
Scientific Reports
·
2020
·Vol. 10
·期 1
·1-10
·
IF 3.998
He Huiqing,Qin Xiangjing,Dong Fangyun,Ye Jingmin,Xu Chunbao,Zhang Hanhui,Liu Zhanmei,Lv Xiaojing,Wu Yuehua,Jiang Xuhong,Cheng Xingan
The invasion of Spodoptera frugiperda has imposed a serious impact on global food security. Matrine is a botanical pesticide with a broad spectrum of insecticidal activity which was recommended for controlling Spodoptera frugiperda . In order to discover effective insecticide for Spodoptera frugiperda , two matrine derivatives modified with carbon disulfide and nitrogen-containing groups were systhesized. And their inhibition activities on Sf9 cell were evaluated. The structural configuration of compounds were characterized by IR, HPLC, MS, NMR and XRD, with yields of 52% and 65%, respectively. The IC 50 of the two newly synthesized compounds on Sf9 cell reduced to 0.648 mmol/L and 1.13 mmol/L, respectively, compared with that of matrine (5.330 mmol/L). In addition, microscopic observation of Sf9 cell treated with the compounds showed that the number of adherent cells decreased, the cells shrunk, vacuolated and apoptotic bodies appeared. The two newly synthesized compounds exhibited better inhibitory effect on Sf9 cell than that of the parent matrine, suggesting that the positive effect of the introduction of 1-pyrrolidinecarbodithioate and diethylcarbamodithioate groups to matrine. The morphological observation of Sf9 cell induced by derivatives indicated that apoptosis induction may be a mechanism that inhibits insect cell proliferation and exerts insecticidal effect.
研究领域:
植物化学
·
昆虫学
·
细胞生物学
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DOI
PubMed
高影响因子
JOURNAL OF ASIA-PACIFIC ENTOMOLOGY
·
2024
·
IF 1.1
Jingjing Zhang,Cuiting Liu,Xinyi Xie,Xuanyue Yu,Yuting Huang,Rui Tang,Jian Zeng,Benshui Shu
The fall armyworm, Spodoptera frugiperda, has become a global invasive pest in recent years, threatening agricultural production. Carvacrol (CAR) is a multifunctional plant-derived compound and exhibited significant bioactivities against many pests. Our previous study found that CAR inhibited growth and development in S. frugiperda . However, the effects of CAR on the midgut of this pest remain unknown. In this study, the actions of CAR on the larval midgut of S. frugiperda were investigated. The results found that 1.0 and 2.0 g/kg CAR treatments destroyed the structure of the larval midgut based on hematoxylin-eosin staining and transmission electron microscope observation. In addition, 2.0 g/kg CAR exposure significantly altered the larval midgut bacterial community composition and structure. The results of the linear discriminant analysis effect size (LEfSe) analysis suggest that six bacterial genera contributed to the different structures of the larval bacterial community. Furthermore, PICRUSt2 analysis found that 16 bacterial function categories were altered by CAR treatment, of which 8 were significantly increased. Our results provided new insight into the toxicological mechanisms of CAR against S. frugiperda larvae and laid the foundation for the field application of S. frugiperda control.
研究领域:
昆虫学
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微生物学
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农业科学
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产品关联
DOI
高影响因子
Plants-Basel
·
2024
·Vol. 13
·期 23
·3300
·
IF 4
Jie Liu,Mariam Tallat,Gensong Wang,Guoping Li,Haowen Zhang,Xujin Wu,Hongbo Qiao,Xincheng Zhao,Hongqiang Feng
The fall armyworm,Spodoptera frugiperda(Lepidoptera: Noctuidae) (FAW), is an invasive and destructive polyphagous pest that poses a significant threat to global agricultural production. The FAW mainly damages maize, with a particular preference for V3–V5 (third to fifth leaf collar) plant stages in northern China. How the FAW moth precisely locates maize plants in the V3–V5 stage at night remains unclear. The aims of this study were to evaluate the visual and olfactory cues used by the FAW to identify its host plant, maize, in order to select attractants with better trapping efficacy. Hyperspectral analysis of maize plants at different growth stages using the ASD Fieldspec 4 spectrometer was performed using mimics (moths or maize leaves sealed with transparent plastic sheets) and black cloth-covered plants for single visual and single olfactory attraction experiments. Gas chromatography–mass spectrometry (GC-MS) was used to analyze volatiles emitted from V3–V5 stage maize leaves. Volatile organic chemicals (VOCs) were screened using electroantennography (EAG) and Y-tube. Attractor efficacy was validated using mimics + VOCs. Results showed very little variance in the spectral reflectance curve of the maize at different growth stages. Fifteen VOCs were identified in the V3–V5 stage leaves of three different maize varieties, of whichcis-3-hexenyl acetate and myrcene were found in relatively high concentrations in these maize varieties. The frequency of visits attracted by single visual stimuli was significantly lower than that attracted by single olfactory or olfactory + visual cues. The attractiveness of foliarcis-3-hexenyl acetate increased as its concentration decreased. The combination of mimics +cis-3-hexenyl acetate (1 ng/μL) increased host detection efficiency and stimulated mating behavior. These results indicate that the nocturnal insect FAW primarily uses olfactory cues for host identification, with visual cues serving as a complementary modality. The synergistic effect of olfactory and visual cues increases the efficiency of host recognition. We found thatcis-3-hexenol acetate at a concentration from maize leaves is a reliable olfactory signal for the FAW. When using host plant VOCs as attractants to control adult FAWs, the role of visual cues must be considered.
研究领域:
昆虫学
·
植物保护
✓ 已验证
产品关联
DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF AGRONOMY
·
2024
·
IF 4.5
Huimin Liao,Zifang Zhou,Yangbeijia Liu,Yuyan Luo,Chenning Zhang,Yuanjiao Feng,Yinghua Shu,Jianwu Wang
It is well-known that increasing crop biodiversity plays an essential role in achieving ecologically based pest management (EBPM); however, the mechanism is poorly understood. The Three Sisters (Maize/Bean/Squash polyculture [MBS]) is a traditional mixed cropping strategy that has been practiced for thousands of years in Mesoamerica; however, mechanistic evidence for its resistance to herbivores is still lacking. In the present study, using both greenhouse and field experiments, we investigated the effects of MBS on the direct and indirect defences of maize ( Zea mays ) against Asian corn borer ( Ostrinia furnacalis ) and fall armyworm ( Spodoptera frugiperda ) caterpillars. MBS significantly improved the direct chemical defences of maize, especially against O. furnacalis , by increasing the content of primary metabolites, secondary metabolites (Benzoxazinoids), and phytohormones ((±)-Jasmonic acid-isoleucine, Jasmonic acid) in maize leaves, thus significantly reducing their survival and relative growth rates. MBS significantly improved the indirect chemical defences of maize by varying the species and content of volatile organic compounds (such as a mixture of nonanal, 1-dodecanol and 2-butyl-1-octanol), seemingly resulting in the avoidance of MBS plants by both herbivores in the Y-tube olfactometer tests. Field experiments further demonstrated that MBS significantly reduced damage to maize leaves by herbivores, enhanced maize yields, and increased the abundance of natural enemies. Therefore, we concluded that MBS improves the resistance of maize to major pests, and can be used as an effective and eco-friendly crop protection strategy in agroecosystems.
研究领域:
农业生态学
·
植物保护学
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产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2015
·
IF 2.912
Rong Wang,Xiaoyan Zhi,Jie Li,Hui Xu
To discover novel natural-product-based pesticidal agents, we prepared a series of oxime sulfonate derivatives of 2′(2′,6′)-(Di)chloropicropodophyllotoxins by structural modification of podophyllotoxin. Their structures were well-characterized by proton nuclear magnetic resonance (1H NMR), high-resolution mass spectrometry (HRMS), optical rotation, and melting point. Moreover, the key steric structure of compound 5f was unambiguously determined by single-crystal X-ray diffraction. Additionally, their insecticidal activity was evaluated at 1 mg/mL against the pre-third-instar larvae of oriental armyworm (Mythimna separata Walker), a typical lepidopteran pest. Among all derivatives, compounds 4c, 5c, and 5d exhibited more promising insecticidal activity, with the final mortality rates greater than 60%, when compared to their precursor podophyllotoxin and the positive control, toosendanin. It demonstrated that introduction of the chlorine atom at the C-2′ or C-2′,6′ position on the E ring of picropodophyllotoxin or oxime sulfonate derivatives of picropodophyllotoxin was important for the insecticidal activity and introduction of a halogen (e.g., fluorine, chlorine, or bromine) atom-substituted phenylsulfonyl group on the oxime fragment of 2′(2′,6′)-(di)chloropicropodophyllones could lead to more promising compounds.
研究领域:
有机化学
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农药科学
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植物保护
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产品关联
DOI
PubMed
高影响因子
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
·
2021
·Vol. 109
·期 4
·e21859
·
IF 1.698
Yan Liu,Shuangyu Li,Hongjia Yang,Yaru Chen,Dong Fan
Carboxylesterases are one of the three major types of detoxification enzyme in insects. In this study, we screened 12 full-length carboxylesterase cDNA sequences from the oriental armyworm Mythimna separata ; they were named MsCarE1–MsCarE12 and registered in GenBank with accession numbers MK440541 – MK440552. Treatment of fourth instar larvae of M. separata with the LD 50 of the insecticide chlorantraniliprole increased the expression levels of MsCarE3 and MsCarE4 , while treatment with the LD 50 of lambda-cyhalothrin significantly increased the expression levels of MsCarE5 and MsCarE10 . Spatiotemporal expression detection showed that MsCarE3 , MsCarE4 , MsCarE5 , and MsCarE10 were expressed at different developmental stages and in different tissues of M. separata and their expression levels were different. Induction using a high dose of chlorantraniliprole resulted in lower expression of MsCarE3 and MsCarE4 . LD 50 of lambda-cyhalothrin induced higher expression of MsCarE5 and MsCarE10 , while LD 70 induced higher MsCarE10 expression at 3, 6, and 12 h after treatment. RNA interference successfully inhibited the expression of MsCarE3 , MsCarE4 , MsCarE5 , and MsCarE10 , to different degrees at different time points. Silencing of MsCarE5 , or MsCarE5 and MsCarE10 simultaneously changed carboxylesterase activity and increased the susceptibility of M. separata larvae to lambda-cyhalothrin. This study provides a new method to increase the insect susceptibility to insecticide. Research Highlights Twelve carboxylesterase cDNA sequences named MsCarE1–MsCarE12 were identified from Mythimna separata. Silence of MsCarE5, or MsCarE5and MsCarE10 simultaneously increased the insecticidal activity of lambda-cyhalothrin on M. separatalarvae.
研究领域:
昆虫学
·
分子生物学
·
害虫管理
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2018
·
IF 3.412
Ruige Yang,Min Lv,Hui Xu
In continuation of our program to discover new potential pesticidal agents, thirty-one piperine analogs containing isoxazoline/pyrazoline scaffold were prepared, and confirmed by infrared spectra, proton/carbon-13 nuclear magnetic resonance spectra, and high-resolution mass spectra. The structures of compounds VIIb and VIIIc were further determined by 1H–1H COSY spectra. Especially the configuration of compound VIIIc was unambiguously confirmed by single-crystal X-ray diffraction. Their pesticidal activities were evaluated against three serious and typically crop-threatening agricultural pests, Tetranychus cinnabarinus Boisduval (spider mite), Mythimna separata Walker (Oriental armyworm), and Plutella xylostella Linnaeus (diamondback moth). Compounds VIIIb and VIIIc exhibited greater than 40-fold more potent acaricidal activity than the lead compound piperine against T. cinnabarinus. Notably, compounds VIa–c exhibited more pronounced oral toxicity against P. xylostella than toosendanin; compounds VIb and VIc displayed more promising growth inhibitory activity against M. separata than toosendanin. It demonstrated that the methylenedioxy and isoxazoline scaffolds were important for the oral toxicity and growth inhibitory activity against P. xylostella and M. separata, respectively; the ethylenedioxy and isoxazoline scaffolds were vital for the acaricidal activity against T. cinnabarinus. Moreover, compounds VIb, VIIf, and VIIIc showed very low toxicity against NRK-52E cells.
研究领域:
有机化学
·
农药学
·
生物化学
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DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2021
·
IF 3.963
Yifan Li,Hong Sun,Hassan Yasoob,Zhen Tian,Yue Li,Ruichi Li,Shengli Zheng,Jiyuan Liu,Yalin Zhang
Cantharidin (CTD) is a natural toxin with effective toxicity to lepidopteran pests. Nevertheless, little information is available on whether pests develop resistance to CTD. After being exposed to CTD (50 mg/L to 90 mg/L) or 10 generations, the resistance ratio of laboratory selected cantharidin-resistant Mythimna separata (Cantharidin-SEL) strain was only elevated 1.95-fold. Meanwhile, the developmental time for M. separata was prolonged (delayed1.65 in males and 1.84 days in females). The reported CTD target, the serine/threonine phosphatases (PSPs), have not been shown significant activity variation during the whole process of CTD-treatment. The activity of detoxification enzymes (cytochrome monooxygenase P450, glutathione-S-transferase (GST) and carboxylesterase) were affected by CTD selection, but this change was not mathematically significant. More importantly, no obvious cross-resistance with other commonly used insecticides was observed in the M. separata population treated with CTD for 10 generations (resistance ratios were all lower 2.5). Overall, M. separata is unlikely to produce target-site insensitivity resistance, metabolic resistance to CTD. Meanwhile, cantharidin-SEL is not prone to develop cross-resistance with other insecticides. These results indicate that CTD is a promising biogenetic lead compound which can be applied in the resistance management on M. separata .
研究领域:
昆虫学
·
害虫管理
·
毒理学
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产品关联
DOI
PubMed
高影响因子
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
·
2023
·Vol. 120
·期 37
·e2308685120
·
IF 11.1
Yu Shi,Qingqing Liu,Wenjie Lu,Jing Yuan,Yihua Yang,John Oakeshott,Yidong Wu
Here, we provide mechanistic support for the involvement of the CYP9A subfamily of cytochrome P450 monooxygenases in the detoxification of host plant defense compounds and chemical insecticides in Spodoptera exigua and Spodoptera frugiperda. Our comparative genomics shows that a large cluster of CYP9A genes occurs in the two species but with significant differences in its contents, including several species-specific duplicates and substantial sequence divergence, both between orthologs and between duplicates. Bioassays of CRISPR-Cas9 knockouts of the clusters show that, collectively, the CYP9As can detoxify two furanocoumarin plant defense compounds (imperatorin and xanthotoxin) and insecticides representing three different chemotypes (pyrethroids, avermectins, and oxadiazines). However, in vitro metabolic assays of heterologously expressed products of individual genes show several differences between the species in the particular CYP9As with activities against these compounds. We also find that the clusters show tight genetic linkage with high levels of pyrethroid resistance in field strains of the two species. We propose that their divergent amplifications of the CYP9A subfamily have not only contributed to the development of the broad host ranges of these species over long evolutionary timeframes but also supplied them with diverse genetic options for evolving resistance to chemical insecticides in the very recent past.
研究领域:
昆虫学
·
分子生物学
·
基因组学
·
毒理学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL ENTOMOLOGY
·
2025
·
IF 1.5
Meng Wei,Zhang Wanming,Zhang Dan,Zheng Jingjun,Huang Dechao,Guo Qianshuang,Du Yongjun
The sex pheromone of the oriental armyworm, Mythimna separata (Walker), in China has always been confusing. It was initially considered as cis-11-hexadecenal (Z11-16:Ald) or cis-11-hexadecenyl acetate (Z11-16:Ac) and cis-11-hexadecenol (Z11-16:OH), as well as a mixture of these compounds. However, inconsistent results have frequently occurred using these compounds or mixtures in field trapping. Through gland analysis from individual females and nationwide field experiments, we identified two distinct types: Z11-16:Ald (aldehyde type or A type) and a mixture of Z11-16:Ac and Z11-16:OH (ester type or E type). Electroantennography (EAG) results indicated that both strains of males co-existed in the field. A strain of males had a strong EAG response to Z11-16:Ald but weak responses to Z11-16:Ac and Z11-16:OH, whereas E strain males had strong responses to Z11-16:Ac and Z11-16:OH but a weak response to Z11-16:Ald. Z11-16:OH identified in female E strain inhibited the attraction of A strain males to A type pheromones. Both A and E types of sex pheromone were respectively formulated and field evaluation (2014-2021) across > 20 provinces from southern to northern China showed geographic and generational variations. During overwintering, E strain males were predominantly caught in Hainan, Fujian, and Guangdong provinces, while A strain males were mainly trapped in Guangxi, Anhui, Sichuan, Jiangsu, and Jiangxi provinces. Subsequent generations exhibited mixed populations in most regions. These findings suggest regional adaptation and migration-driven hybridization. The study highlights the necessity of strain-specific pheromone blends for effective monitoring and control. This work resolves long-standing ambiguities in M. separata sex pheromone ecology.
研究领域:
昆虫学
·
化学生态学
·
信息素研究
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种群遗传学
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农业害虫治理
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF INSECT PHYSIOLOGY
·
2025
·
IF 2.3
Xu Wu,Wen-Mei Li,Qiang Xu,Xiao-Ping Yi,Da-He Zhang,Ming-Zhe He,Yu-Hong Ren
RNA interference (RNAi) has shown excellent potential for pest management worldwide. However, the application of RNAi against insects can be inefficient and unreliable. Finding a better delivery system is a crucial factor for enhancing RNAi efficacy. Here, we present a novel and effective approach of conjugating double-stranded RNA (dsRNA) with a cell-penetrating disulfide polymer (CPD) to improve dsRNA stability and RNAi efficiency. The fall armyworm (FAW), Spodoptera frugiperda , is a globally agricultural insect. Chitin synthase B ( CHSB ) and methoprene-tolerant ( Met ) genes, which are essential for the development and growth of FAW, were selected as target genes. The CPD was synthesized using a two-step method for dsRNA delivery. The synthesized CPD/dsRNA complex protected dsRNA from nuclease degradation. The biological application of CPD in Sf9 cells demonstrated low cytotoxicity and high cell viability. Moreover, the CPD-loaded dsRNA entered the cells within 6 h. Bioassays of FAW showed that the relative expression levels of the CHSB and Met genes were reduced. Specifically, at 72 h, the relative expression levels of the CHSB and Met genes were 48.14 % and 37.60 % of those in the control group, respectively. The weight and body length of the larvae decreased significantly, and the mortality rate of CPD/ds CHSB reached 30 %. This CPD was demonstrated for the first time to have excellent delivery performance in insects and is expected to become a new and effective tool for pest control, representing a significant advancement in pest management.
研究领域:
昆虫学
·
分子生物学
·
害虫治理
·
生物技术
·
RNA干扰技术
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）