Journal of Materials Chemistry B
·
2024
·
IF 6.1
Fengyuan Liu,Jiashan Xia,Chun Tao,Changmei Chen,Xiangshu Cheng,Rongchun Yi,Lulu Wang,Yue Wang,Tao Deng
Chirality is a ubiquitous phenomenon in nature. The advent of nanomaterials has led to a gradual evolution of chiral studies from the molecular scale to the nanoscale. The emergence of carbon dots (CDs) has inaugurated a novel domain in the scientific and technological realms of carbon nanomaterials. In recent years, CDs have attracted increasing attention due to their luminescent properties, excellent biocompatibility and remarkable bioactivity. However, little attention has been paid to the chirality of the CDs and, in particular, the differences between CDs synthesized from racemic compounds and other chiral CDs, as well as the differences in the biological effects of chiral CDs, remain to be explored. Here, chiral CDs were synthesized from L-/D-/DL-arginine, and the differences in various aspects of chiral CDs were evaluated. We found that L-CDs without extreme differences in structure had brighter fluorescence and a more significant ability of NO generation and lipid droplet inhibition than the other two chiral CDs. Combined with its better drug loading and release ability, we validated its superior efficacy in tumor treatment. Therefore, this study provides a basis for further research on chiral carbon dots and their differences.
研究领域:
纳米材料
·
生物技术
·
生物化学
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMEDICINE & PHARMACOTHERAPY
·
2019
·
IF 3.743
Lu Gao,Jia-Hua Zhang,Xiao-Xu Chen,Hui-Li Ren,Xiu-Ling Feng,Jia-Ling Wang,Jun-Hua Xiao
Pulmonary fibrosis (PF) progression may be involved with arginine (Arg) metabolism and immune balance. The present study aimed to explore the effects of L-Arginine (L-Arg) and L-Norvaline (L-Nor) on bleomycin (BLM)-induced PF in mice, meanwhile, and observe dynamic changes of Arg metabolism, immune balance and crosstalk between them in PF progression. Followed intratracheal instillation of BLM or saline, Kunming mice were treated orally with saline, L-Arg, L-Nor and L-Arg + L-Nor three times a day. And the mice were sacrificed on Day 3, 14 and 28 after treatment. Changes of body weight, lung index, lung hydroxyproline and histopathology were analyzed to evaluate the PF degree. Peripheral blood Arg, Citrulline (Cit), Ornithine (Orn) and Proline (Pro), lung NO, NOS and arginase were analyzed to evaluate the Arg metabolism. Peripheral blood Tregs, Th17 and γδT cells were analyzed to evaluate the immune balance. Our data showed that combination of L-Arg and L-Nor dynamically reversed the weight loss, decreased lung index and hydroxyproline, and improved lung histopathological damages induced by BLM. The combination dynamically and significantly rectified Tregs, Th17, γδT and Tregs/Th17 abnormal changes. Meanwhile, these disorders of peripheral blood Arg, Cit, Orn, Pro, Orn/Cit and Pro/Orn, and lung NO, iNOS and TNOS were also improved accordingly. These results demonstrated that combination of L-Arg and L-Nor had inhibitory effects on BLM-induced PF progression, possibly due to their corrective action on immune imbalance, Arg metabolism disorder and crosstalk abnormality in the progression of PF.
研究领域:
药理学
·
免疫学
·
肺病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD ENGINEERING
·
2022
·
IF 6.203
Jun Qi,Hui-min Yan,Wen-wen Zhang,Xiu-ning Yao,Qing-yuan Cai,Kuo Xu,Wen-ping Xu,Guo-yuan Xiong
Effects of pH (5, 7, or 9) and arginine level (0, 0.6%, 1.2%, or 1.8%, w/v) on the stabilization of emulsions prepared by a novel thermal-assisted stirring method were evaluated. The results of physical stability showed that thermal-assisted stirring was effective in manufacturing heat-insensitive o/w emulsions. As the arginine level increased, the emulsifying ability and the absolute zeta potential of each pH-treated group first increased significantly and then decreased significantly, reaching a maximum at 1.2% (w/v) addition. When pH increased, the emulsifying ability and the absolute zeta potential of each arginine-treated group increased significantly. The results of particle size, storage modulus, and loss modulus were in accordance with changes in the emulsifying ability and the zeta potential, which were confirmed by the microstructure. As a result, the emulsion prepared at pH 9 with 1.2% (w/v) arginine had the largest levels of emulsion stability and surface protein load (145.69 mg/m 2 ). In conclusion, the major reason for the formation of heat-insensitive emulsions by thermal-assisted stirring might be attributed to a high level of surface protein load, and increasing pH and arginine addition might enhance the adsorption of gelatin-arginine complexes on the surface of oil droplets, thereby improving the stability of emulsions.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
MEDICINE
·
2018
·
IF 2.028
Meijia Wu,Ting Huang,Juan Wang,Ping Chen,Wanwan Mi,Yuanyuan Ying,Hangli Wang,Dandan Zhao,Shengwu Huang
Supplemental Digital Content is available in the text
研究领域:
药学
·
肿瘤学
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
CURRENT MICROBIOLOGY
·
2023
·Vol. 80
·期 9
·1-9
·
IF 2.6
Jiao Yu Liang,Shen Pin Quan,Wang Shu Fang,Chen Jing,Zhou Xiang Hong,Ma Gui Zhen
Arginase has shown promising potential in treating cancers by arginine deprivation therapy; however, low enzymatic activity and stability of arginase are impeding its development. This study was aimed to improve the enzymological properties of a marine bacterial arginase by carboxymethyl chitosan (CMCS) conjugation. An arginase producing marine bacterium Priestia megaterium strain P6 was isolated and identified. The novel arginase PMA from the strain was heterologously expressed, purified, and then conjugated to CMCS by ionic gelation with calcium chloride as the crosslinking agent. Enzymological properties of both PMA and CMCS-PMA conjugate were determined. The optimum temperature for PMA and CMCS-PMA at pH 7 were 60 °C and 55 °C, respectively. The optimum pH for PMA and CMCS-PMA at 37 °C were pH 10 and 9, respectively. CMCS-PMA showed higher thermostability than PMA over 55–70 °C and higher pH stability over pH 4–11 with the highest pH stability at pH 7. At 37 °C and pH of 7, i.e., around the human blood temperature and pH, CMCS-PMA was higher than the free PMA in enzymatic activity and stability by 24% and 21%, respectively. CMCS conjugation not only changed the optimum temperature, optimum pH, and enzymatic activity of PMA, but also improved its pH stability and temperature stability, and thus made it more favorable for medical application.
研究领域:
生物化学
·
生物技术
·
药学
·
癌症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
AMINO ACIDS
·
2025
·Vol. 57
·期 1
·1-15
·
IF 2.4
Wang Jiteng,Zhang Jingyi,Li Xinyu,Xu Hanying Y.,Yang Yeshun,Zhang Jiankun,Feng Wenping,Chen Qiang,Dong Fen,Han Tao
The main objective of this experiment was to study the metabolism of arginine in juvenile largemouth bass ( Micropterus salmoides ). A total of 300 healthy fish (average weight of 25 ± 0.5 g) were randomly assigned to ten groups. Experimental fish were orally administered or intraperitoneally injected with 0.9% sodium chloride, arginine, arginine-aspartate, citrulline, and glutamate solutions, respectively. They were euthanized at 10, 30, 60, 120, and 240 min after oral administration or intraperitoneal injection, and various tissue samples were subsequently collected for analysis. The results revealed that serum ornithine and citrulline concentrations of largemouth bass were significantly increased by oral administration of arginine or arginine-aspartate ( P < 0.05). Intraperitoneal injection of arginine or arginine-aspartate solution significantly elevated the concentrations of ornithine and citrulline in the serum, liver, kidney, and muscles ( P < 0.05). The concentrations of citrulline, ornithine, and arginine in serum and muscle increased significantly at 4 h after intraperitoneal injection of glutamate ( P < 0.05). Intraperitoneal injection of citrulline significantly increased the concentrations of ornithine and arginine in the serum and muscles ( P < 0.05). The research findings demonstrate that both free and small peptide forms of arginine were rapidly degraded to ornithine due to the high arginase activity in various tissues of largemouth bass. Additionally, the pathway of synthesizing citrulline from glutamate and then arginine from citrulline may exist in largemouth bass, but the exact location of this synthesis process may differ from that found in mammals.
研究领域:
水产养殖学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD PROCESSING AND PRESERVATION
·
2021
·Vol. 45
·期 10
·e15821
·
IF 2.19
Qun Huang,Hanliang Song,Yonggui Pan,Zhengke Zhang
Papaya fruit is easily affected by low temperature, causing chilling injury (CI). In this study, the exogenous arginine was applied in the storage of postharvest papaya fruit and it was found that 0.8 mM arginine treatment significantly alleviated CI of papaya fruit. Arginine treatment promoted the accumulation of polyamines (PAs), which might be ascribed to the increased activities of arginase, arginine decarboxylase (ADC), ornithine decarboxylase (ODC), and S-adenosylmethionine decarboxylase (SAMDC). A higher accumulation of proline was found in arginine-treated papaya fruit, which may be due to increased activity of ornithine-δ-aminotransferase (OAT) and Δ 1 -pyrroline-5-carboxylate acid synthase (P5CS) and inhibited activity of proline dehydrogenase (PDH). In addition, arginine treatment increased higher nitric oxide synthase (NOS) activity and promoted the generation of nitric oxide (NO). The results of this study indicate that exogenous arginine may improve the chilling tolerance of postharvest papaya fruit by regulating arginine and proline metabolism. Practical applications In recent years, chilling injury of tropical fruits is a significant problem, including papaya. Many studies on polyamine, proline, nitric oxide, and chilling injury of fruits, but arginine, as a common precursor of polyamine, proline, and nitric oxide, are rarely studied, especially in papaya fruit. Our findings demonstrated that the exogenous arginine can regulate endogenous arginine metabolism, induce polyamine, putrescine, and nitric oxide accumulation, and coordinate to alleviate chilling injury of papaya fruit.
研究领域:
植物生理学
·
园艺学
✓ 已验证
产品关联
DOI
高影响因子
Animal Nutrition
·
2025
·
IF 7.5
Jing Zhang,Jiaojiao Lang,Lijun Bu,Yapeng Liu,Wenjie Huo,Caixia Pei,Qiang Liu
Arginine (Arg) can potentially increase milk yields in early lactating dairy cows by stimulating the development of mammary gland and the biosynthesis of milk proteins and fatty acids (FA); however, the underlying mechanism is still unclear. The hypothesis was detected that rumen-protected Arg (RPArg) addition would increase the yields of milk, milk fat, and milk protein and aimed to elucidate the possible mechanism. Forty-eight multiparous Chinese Holstein dairy cows (milk yield 34.9 ± 1.27 kg/d, body weight 658 ± 12.7 kg, and days in lactation [DIL] 16.4 ± 2.56) were selected on the principle of proximity and blocked by DIL and milk yield, and divided into one of four groups in a randomized block design: control (without RPArg), low RPArg (LRPArg, 20 g/d Arg), medium RPArg (MRPArg, 40 g/d Arg), and high RPArg (HRPArg, 60 g/d Arg), 4 replicates per group and 3 cows per replicate the experiment duration was 95 days, including a 10-day covariate period, a 15-day adaptation period, and a 70-day sampling period. The production of actual milk, energy-corrected milk, fat-corrected milk, milk fat, and milk protein linearly increased ( P < 0.05) with RPArg provision. The yields of de novo FA and mixed FA in the milk linearly increased ( P < 0.01), but that of preformed FA linearly decreased ( P = 0.007). Supplementation with RPArg linearly increased the daily production of Arg, Asp, Cys, Glu, His, Met, Phe, Ser, Thr, and Trp ( P < 0.05). With RPArg provision, serum contents of glucose, albumin, total protein, and nitric oxide linearly increased ( P < 0.05); the levels of estradiol, prolactin, and insulin-like growth factor 1 quadratically increased ( P < 0.01); and that of blood urea nitrogen quadratically decreased ( P = 0.023). Furthermore, supplementation with 40 g/d Arg from RPArg promoted the expression of proteins implicated in mammary gland proliferation, FA synthesis, and milk protein synthesis. Thus, RPArg addition enhanced milk production, FA synthesis, and milk protein synthesis by promoting the expression of proteins involved in mammary gland development, FA synthesis, and milk protein synthesis.
研究领域:
动物营养学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Xuanqi Hao,Xiao Zhang,Jianrong Wang,Chenchen Liu,Chuanhao Li,Ping Yang
To create fluorescent sensors for Cr (VI) and l-arginine detection, blue-emitting carbon dots (CDs, named as M-CDs) were prepared via a solvothermal synthesis by using m-phenylenediamine. The introduction of sulfuric acid changed the blue-emitting of CDs into green-emitting (named as S-CDs), in which the photoluminescence (PL) peak wavelengths of samples M-CDs and S-CDs were 450 and 540 nm, while their PL efficiencies were 21.7 and 84.2 % respectively. Sulfuric acid resulted in the average fluorescent lifetime of the CDs was prolonged into 8.19 from 4.65 ns. The photo and thermal stable tests demonstrated that theses CDs revealed excellent stability. Meanwhile, CDs flexible films with bright blue- and green-emitting were prepared utilizing the favorable solubility of CDs in polar solvents. Mono-dispersed CDs as luminescent materials demonstrated it promising prospects in luminescent devices field. Furthermore, M-CDs exhibited excellent selectivity towards Cr(VI) and l-arginine. The detected limit (0.86 μM for Cr(VI) and 0.43 μM for l-arginine) was determined by assessing the response of M-CDs to various concentrations of Cr(VI) ion and l-arginine solutions. These bright CDs revealed great potential for accurate detection of Cr(VI) and l-arginine.
研究领域:
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Marine Drugs
·
2022
·Vol. 20
·期 11
·688
·
IF 6.085
Jingmin Cui,Yan Sun,Linqing Wang,Qin Miao,Wenqiang Tan,Zhanyong Guo
We successfully prepared a series ofl-arginine Schiff bases acylated chitosan derivatives, aiming to improve the antioxidant activity and antimicrobial activity of chitosan by introducing a furan ring, pyridine ring, andl-arginine structure. The accuracy of the structures of ten compounds was characterized by FT-IR and1H NMR. In terms of DPPH radical scavenging activity, except for compound CR3PCA, the scavenging rate of other compounds was higher than chitosan, especially CRCF and CRBF had strong scavenging abilities. At the same time, in the superoxide-radical scavenging activity assay, CRCF, CRBF, CR3PCA, CR2C3PCA, and CR2B3PCA were comparable to positive control at 1.60 mg/mL. Simultaneously, CRFF, CRCF, and CRBF had a certain inhibitory effect onBotrytis cinerea. Furthermore, the inhibitory effect of CRFF, CRCF, and CR3PCA onStaphylococcus aureuswas very well, close to the positive control at 1.00 mg/mL. CRCF and CR2B3PCA showed better inhibitory effects onEscherichia colithan other compounds. The MTT assay was used to determine the cytotoxicity of the chitosan derivatives, which proved their safety to fibroblast cells. In summary, the study indicated that some of these compounds have the potential for further development and utilization in the preparation of antioxidants and antimicrobial agents.Keywords:chitosan;l-arginine Schiff bases;heterocyclic compounds
研究领域:
生物化学
·
食品科学
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Mingyue Zhao,Xin Liu,Yuzhu Hou,Tongtong Yang,Jiaquan Xu,Rui Su
The levels of l-arginine and asymmetric dimethylarginine (ADMA) and the amount of the nitric oxide (NO) production have recently been linked to breast cancer and pharmaceutical effect evaluation. Herein, a method combining electrochemistry and high-resolution mass spectrometry (HRMS) was established and used to study NO metabolism and its modulation by ginsenoside compound K (CK) in breast cancer cells. Platinum nanoparticles-decorated fluorine tin oxide was employed as an electrochemical sensor for in situ detection of NO release, while HRMS was used for the analysis of the NO-related metabolites. Through the combination of the electrochemical and HRMS results, decreases in arginine and NO and increases in ADMA and ornithine were observed after modulation by CK, and two highly correlated metabolic pathways including arginine and proline metabolism and vascular smooth muscle contraction were found. This method offers a new strategy for fast evaluation of pharmaceutical efficacy based on NO metabolism.
研究领域:
分析化学
·
生物化学
·
药理学
·
癌症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2017
·Vol. 41
·期 24
·15216-15228
·
IF 3.269
Xiaoqing Zeng,Lei Zhang,Jidong Yang,Yuan Guo,Yunmei Huang,Haiyan Yuan,Yongsheng Xie
In this report, water-soluble carbon dots (CDs) with stable fluorescence (FL) were designed and synthesized by using environment-friendly reduced L-glutathione (GSH) as the precursor and ethylenediamine (EDA) as the passivating agent through a one-step hydro-thermal method. The CDs, with bright blue FL, had the quantum yield (QY) of 40% and showed excellent monodispersity and solubility in water. The FL intensity was remarkably enhanced when a racemic modification of L-/D-arginine (Arg) was added into the CDs solution. Therefore, the CDs could be considered as a sensitive probe for the detection of Arg. Under optimized conditions, the CDs exhibited a linear fluorescence response in the range of 3–124 μM (R2 = 0.9987) Arg with the detection limit of 2.85 × 10−8 M. In addition, no evident difference was observed when comparing the FL results of L-Arg and D-Arg. Therefore, this method could not be used in the chiral recognition of two enantiomers. Under optimum conditions, the mechanism and the reasons for the enhancement of FL were investigated. The developed method exhibited great stability, selectivity and accuracy in determining the Arg content in human urine sample and the recovery range was from 97.0% to 102.2%. Thus, our proposed method has potential application in biological molecular recognition.
研究领域:
分析化学
·
生物化学
·
医学诊断
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）