BIORESOURCE TECHNOLOGY
·
2016
·
IF 4.917
Qing Qing,Linlin Zhou,Qi Guo,Meizi Huang,Yucai He,Liqun Wang,Yue Zhang
Na 3 PO 4 and Na 2 S were employed as efficient alkaline catalysts for the pretreatment of corn stover. To systematically obtain optimal conditions, the effects of critical pretreatment parameters including sodium phosphate concentration (1–4%), sulfidity (0–20%), pretreatment temperature (100–120 °C), and reaction time (20–60 min) on the reducing sugar yield of pretreated substrates were evaluated in a lab-scale using the response surface methodology. Pretreated under the sodium phosphate concentration of 4%, sulfidity of 10%, temperature of 120 °C, and reaction time of 40 min, the reducing sugar yield and glucose yield of the pretreated corn stover achieved 91.11% and 64.01%, respectively, with a moderate enzyme loading of 30 FPU/g substrate. Additionally, a strong correlation (R 2 = 0.971 and R 2 = 0.954) between the delignification and the reducing sugar yield (or glucose yield) was observed by this pretreatment method. These results evidently support that the combined Na 3 PO 4 -Na 2 S pretreatment is an effective and feasible method for processing lignocellulosic biomass.
研究领域:
生物技术
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生物质能
·
酶解技术
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2021
·
IF 5.645
Hao Zhou,Qin Liu,Xiuhong Zhong,Yaqi Chu,Zhaojiang Wang,Yanqin Wang
To achieve the valorization of whole compositions of lignocellulosic biomass, flow fractionation (FF) was developed based on the flow chemistry of the major components of biomass, i.e., glucan, xylan, and lignin. Three kinds of solvent, i.e., water, formic acid, and p -toluenesulfonic acid (p-TsOH), were employed in FF treatments of poplar wood for an evaluation of performance in structure and yield of products. The advantage of FF was firstly confirmed by the nearly complete recovery of glucan in solid product, and the greatly enhanced digestibility of enzymatic hydrolysis as indicated by the glucose yield of 68.1 % at a low cellulase dosage of 10 FPU/g solid product from water treated poplar wood. The second advantage of FF was the rapid hydrolysis and very slight degradation of xylan for the three solvents explored, especially for water as indicated by the xylose yield of 80.3 % from poplar wood treated at 200 °C just for 15 min.. Last but not least, structure-preserved lignins were harvested from biomass with good yield, e.g., 40.3 % of lignin yield and 99.8 % of β-O-4 retention from poplar wood using water as solvent. Thus-obtained lignin (F72T130t30) was successfully transformed to hydrocarbons over Ru/Nb 2 O 5 catalyst with the yield of 21 wt%. In short, these remarkable performances of FF in products structure and yield build a feasible valorization of lignocellulosic biomass for sustainable biorefinery.
研究领域:
生物化学工程
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可再生资源与环境科学
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产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Meizhen Jiao,Jie Lu,Jinguang Hu,Jian Du,Yanna Lv,Jinwen Hu,Yehan Tao,Haisong Wang
In this study, we introduce a novel two-step method for efficiently separating and utilizing reed biomass. The process involves seawater and ammonium chloride (SW/AC) pretreatment followed by high solid-phase ethanolamine (ETA) pretreatment. During the SW/AC pre-processing stage, metal ions and chloride ions in seawater facilitated the depolymerization of hemicellulose into xylo-oligosaccharides (XOS), yielding 44.71 %. Subsequently, the two-step SW/AC-ETA pretreatment achieved a lignin removal rate of 80.34 % and rose to yields of glucose (99.10 %) and xylose (84.95 %). Characterization via FTIR, XRD, and SEM analyses revealed significant disruption of the lignin-carbohydrate complex structure in the reed, thereby increasing cellulose accessibility from 136.99 mg/g in raw material to 526.32 mg/g. This study offers a novel, environmentally friendly, and efficient strategy for separating reed components.
研究领域:
生物化学工程
·
可再生资源利用
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产品关联
DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2017
·
IF 5.651
Hongdan Zhang,Shuaishuai Zhang,Hongyou Yuan,Gaojin Lyu,Jun Xie
An organosolv pretreatment system consisting of 60% ethanol and 0.025 mol·L −1 FeCl 3 under various temperatures was developed in this study. During the pretreatment, the highest xylose yield was 11.4 g/100 g raw material, representing 49.8% of xylose in sugarcane bagasse. Structural features of raw material and pretreated substrates were characterized to better understand how hemicellulose removal and delignification affected subsequent enzymatic hydrolysis. The 160 °C pretreated solid presented a remarkable glucose yield of 93.8% for 72 h. Furthermore, the influence of different additives on the enzymatic hydrolysis of pretreated solid was investigated. The results indicated that the addition of Tween 80 shortened hydrolysis time to 6 h and allowed a 50% reduction of enzyme loading to achieve the same level of glucose yield. This work suggested that FeCl 3 -catalyzed organosolv pretreatment could improve the enzymatic hydrolysis significantly and reduce the hydrolysis time and enzyme dosage with the addition of Tween 80.
研究领域:
生物技术
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化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Inorganic and Organometallic Polymers and Materials
·
2023
·Vol. 33
·期 7
·2000-2012
·
IF 4
Gao Die,Zhu Qingqing,Liu Peng,Zhou Qing,Cheng Xiujie,Liu Li,Xu Junli,Lu Xingmei
Proton-type ionic liquids have been proved to be promising for the pretreatment of lignocellulosic biomass, because they can provide high yield cellulase hydrolysis and high lignin removal rate. In this work, three proton-type ionic liquids, ethyl methanesulfonamide (EAM), ethyl amine nitrate (EAN) and ethyl amine acetate (EAN[OAc]), were successfully synthesized to pretreat corn straw, and five sugar platform organic solvents were selected as co-solvents for biomass pretreatment. The experimental results showed that under the optimal pretreatment conditions (10 wt% EAM, 130 °C, 2 h, 7 wt% straw loading in PDO solvent), the delignification rate of corn straw could reach 96 wt%, the sugar yield of cellulose-rich substrate could reach 95 wt% in 96 h, the regeneration rate of lignin dissolved in the solvent was 86 wt%, and the remainder of the solvent was small molecular compound, the hemicellulose dissolved in the solvent is degraded to monomolecular sugars (xylose and arabinose). The XRD, SEM characterization data and spectrograms of cellulosic substrate also fully proved that the lignin was successfully dissolved and separated from corn straw by pretreatment of composite system, and in the remaining straw the content and crystallinity of cellulose was improved. In this paper, density functional theory (DFT) is used to explore the pretreatment principle. From the optimized structure, it can be seen that the interaction energy between the ionic liquid cosolvent system and lignin is higher than that in the pure ionic liquid system. The simulation results, experimental analysis and characterization can effectively explain the essence of the system for efficient straw pretreatment, and the system can provide new ideas for the development of biomass pretreatment.
研究领域:
生物化学
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可再生资源与环境
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产品关联
DOI
高影响因子
Biomass Conversion and Biorefinery
·
2024
·
IF 3.5
Wang Xuchong,Wei Yuhe,Sun Xiaoping,Yuan Juan,Chen Haoqiu,Sun Yanfen,Luo Lianxin
Pretreatment is the key step in enzymatic hydrolysis of bamboo wood. Green liquor pretreatment is a promising method to improve enzymatic hydrolysis and saccharification efficiency by changing the physical and chemical properties of lignocellulose surface. At present, there is little work to quantitatively analyze the enzymatic saccharification rate of bamboo lignocellulose by two-stage pretreatment. In this study, a two-stage pretreatment method combining hot water and green liquid phase was mainly used, and the bamboo was treated with Bovine serum albumin (BSA) and Tween80 (TW) chemical additives. After that, the treated samples were hydrolyzed by cellulase, and the effects of pretreatment on the chemical composition and physical properties of bamboo and the influencing factors of chemical additives on the enzymatic hydrolysis efficiency of bamboo were explored. The results showed the two pretreatment stages of hot water and green liquid to have an evident effect on the enzymatic hydrolysis of bamboo. The two-stage pretreatment with hot water and green liquid (14% total alkali) resulted in an 85.51% yield of total sugar hydrolyzed by the enzyme. The addition of BSA and Tween80 improved the efficiency of the enzymatic hydrolysis of bamboo, and the synergistic effect of them could make the total sugar yield of enzymatic hydrolysis reach 99.67%. Through calculation, 65.88 g of fermentable sugar can be obtained per 100 g of bamboo fiber through enzymatic hydrolysis with cellulase. Graphical Abstract
研究领域:
生物化学
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可再生资源与环境
✓ 已验证
产品关联
DOI
高影响因子
CHINESE JOURNAL OF CHEMISTRY
·
2020
·Vol. 38
·期 4
·399-405
·
IF 3.826
Ying Qiao,Lixin Feng,Zhongqiu Li,Zhenyu Zhang,Jing Chen,Haining Na,Jin Zhu,Lai Chen
Summary of main observation and conclusion Five types of ZrO 2 are used to simply establish an effective aqueous catalytic system with sulfuric acid for the hydrolysis of cellulose to sugar, respectively. By adjusting surface roughness and particle size of ZrO 2 , different adsorption is produced. Accordingly, following the thought to inhibit the self-aggregation nature of cellulose, after the introduction of ZrO 2 with adsorption in catalysts, the efficiency of the hydrolysis of cellulose to sugar is improved. The results show different ZrO 2 can produce different effects during the hydrolysis of the cellulose with relatively low crystallinity. Under the optimal conditions, the conversion of cellulose and TRS yield reaches 95.5% and 93.6%, respectively. Through this research, it reveals the critical point to organize a feasible and useful way for the hydrolysis of cellulose to sugar with high efficiency in an aqueous system. An important theoretical and technical support is also provided for the conversion and utilization of cellulose in the future.
研究领域:
生物化学
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可再生能源
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Xing Zhu,Yuanyuan Qiang,Xuechuan Wang,Mingliang Fan,Zuoyuan Lv,Yi Zhou,Bin He
Immobilized enzymes are one of the most common tools used in enzyme engineering, as they can substantially reduce the cost of enzyme isolation and use. However, efficient catalysis of solid substrates using immobilized enzymes is challenging, hydrolysis of insoluble cellulose by immobilized cellulases is a typical example of this problem. In this study, inspired by bees and honeycombs, we prepared gelatin-modified cellulase (BEE) and gelatin hydrogels (HONEYCOMB) to achieve reversible recycling versus release of cellulase through temperature-responsive changes in the triple-stranded helix-like interactions between BEE and HONEYCOMB. At elevated temperatures, BEE was released from HONEYCOMB and participated in hydrolytic saccharification . After 24 h, the glucose yields of both the free enzyme and BEE reached the same level. When the temperature was decreased, BEE recombined with HONEYCOMB to facilitate the effective separation and recycling of BEE from the system. The enzymatic system retained >70 % activity after four reuse cycles. In addition, this system showed good biocompatibility and environmental safety. This method increases the mass transfer capacity and enables easy recovery of immobilized cellulase, thereby serving as a valuable strategy for the immobilization of other enzymes.
研究领域:
生物化学
·
酶工程
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产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2017
·
IF 5.651
Xiaojing Lv,Jianghai Lin,Liang Luo,Dou Zhang,Senlin Lei,Wenjuan Xiao,Yuan Xu,Yingxue Gong,Zehuan Liu
Sodium methoxide ( CH 3 ONa) with glycerol pretreatment (CWGP) was performed to improve the enzymatic digestibility of sugarcane bagasse (SCB). Response surface methodology was utilized to optimize the CWGP parameters for pretreating SCB from the perspective of total fermentable sugar yield (TFSY) and total fermentable sugar concentration (TFSC). Under the optimal CWGP conditions, 0.5666 g/g of TFSY (0.82% CH 3 ONa, 1.11 h, 150 °C) and 17.75 g/L of TFSC (0.87% CH 3 ONa, 1.38 h, 149.27 °C) were achieved, corresponding to delignification of 79.05% and 79.34%, respectively. Compared the pretreatment using glycerol or CH 3 ONa alone, the CWGP has significant synergies to enhance the enzymatic efficiency of SCB. The physical and chemical characteristics of untreated and pretreated SCBs were analyzed using FT-IR, XRD, and SEM, and the results suggest that CWGP significantly increased the susceptibility of the substrates to enzymatic digestibility. Ultimately, CWGP might be a prospective candidate for the pretreatment process of enzyme-based lignocellulosic biorefineries.
研究领域:
生物化学
·
可再生能源
·
生物质转化
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2021
·
IF 5.645
Meishan Fan,Chenbiao Zhao,Xinyu Huang,Hongdan Zhang,Jun Xie
In this study, various combined pretreatment strategies using liquid hot water (LHW), 60 % ethanol, 1% H 2 SO 4 and 0.5 % NaOH together with different surfactants (PEG 6000, Triton X-100 and Tween 80) were evaluated to improve glucose production from sugarcane bagasse (SCB) during enzymatic hydrolysis. After pretreatment with PEG 6000-assisted 1% H 2 SO 4 , the highest glucose yield of 75.04 % was achieved. Pretreatments of SCB with various combinations of H 2 SO 4 (1–3%, w/v) and PEG 6000 (0–1.5 %, w/v) were further investigated for enhancing enzymatic hydrolysis and ethanol production. When 1.5 % PEG 6000 was introduced during pretreatment with 2 % H 2 SO 4 , the highest glucose yield of 86.14 % and ethanol concentration of 20.17 g/L were liberated, which were much higher than those obtained from pretreatment with 2% H 2 SO 4 alone. This increased digestibility and fermentability can be attributed to decreases in crystalline sizes and PEG 6000 adherence to SCB surfaces, which was confirmed by cellulase adsorption isotherms, FT-IR, XRD, and SEM analysis. In addition, prehydrolysate and the stillage remaining after ethanol production were sequentially digested to seek potential biomethane production and resulted in final methane yields of 264.05 mL and 387.77 mL per g of volatile solids, respectively. The feasibility of the process provids an environmentally friendly technology for an integrated biorefinery.
研究领域:
生物技术
·
可再生能源
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
CELLULOSE
·
2024
·
IF 4.9
Paiva Mateus F.,Albuquerque Elise M.,de Souza Priscilla M.,Bitter Johannes H.,Vanhove Guillaume,Wojcieszak Robert,Noronha Fábio B.
Increasing the efficiency of cellulose hydrolysis is a crucial milestone to enable its use as a platform for the production of renewable chemicals. Consequently, exploring alternative techniques for cellulose dissolution and subsequent depolymerization and hydrolysis remains a pivotal area of research. This study delves into the performance of diverse solid acid catalysts (ion-exchange resins, metal oxides, and zeolites) for cellulose depolymerization when combined with a concentrated solution of zinc chloride (ZnCl 2 ) as the reaction medium and extractant. The influence of this molten salt hydrate on cellulose dissolution and the stability of the tested catalysts was thoroughly investigated. Optimal mild operating conditions (90 °C and 2 h) that facilitate enhanced conversion and selective glucose production were identified. The highest glucose yields (exceeding 40%) were obtained with Amberlyst-15 and HZSM-5 (SiO 2 /Al 2 O 3 = 23), effectively mitigating product degradation and equaling or surpassing the traditional homogeneous system using H 2 SO 4 . Furthermore, insights into the deactivation mechanisms affecting the best catalysts are provided.
研究领域:
生物化学
·
可再生能源
✓ 已验证
DOI
高影响因子
RENEWABLE ENERGY
·
2022
·
IF 8.634
Yanting Lv,Zhengyu Chen,Huan Wang,Yongcang Xiao,Rongxin Ling,Murong Gong,Weiqi Wei
In this work, an HCl-catalyzed ethylene glycol (EG) pretreatment was exploited for promoting sugar release from sugarcane bagasse (SCB). The results showed that the combination of HCl and EG could remove the xylan (∼100.0%) and lignin (∼61.3%) in SCB together, which finally resulted in the pretreated substrate having a good efficacy for follow-up enzymatic hydrolysis . The maximum glucose yield of this work was 93.9% and obtained after pretreatment at 130 °C for 60 min with 0.5% HCl and 72 h enzymatic digestion. The analysis of various pretreated or un-pretreated SCB indicated that the changes of surface morphology and internal compositions of SCB were the mainly reasons for its hydrolysis efficiency enhancement. The addition of Tween 80 into hydrolysis process could remarkably shorten hydrolysis time and cellulase dosage from 72 h to 36 h and 20 FPU/g substrate to 10 FPU/g substrate, respectively, meanwhile maintaining a relatively high glucose yield (91.4%).
研究领域:
生物技术
·
可再生资源工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）