ChemistrySelect
·
2024
·Vol. 9
·期 11
·e202400114
·
IF 1.9
Jin Liang,Xiaoxin Wang,Dongsheng Yu,XiTao Luo,Xiangfeng Liang,Zhongliang Su,Qinghui Shou
Graphical Self-supported osmotic membranes were prepared by blending PIM-1-mPEG, which was obtained by modifying PIM-1 with polyethylene glycol monomethyl ether (mPEG), and hydrophilic Tröger's base polymer (DMBP-TB). The membranes utilized microporous molecular sieves and the differences in solute-membrane interactions to achieve high pentose/hexose selectivity in aqueous solutions. Monosaccharides are vital building blocks in bioengineering applications; however, their extraction from intricate mixtures is challenging and uses substantial amounts of energy. Polymers of intrinsic microporosity (PIMs) offer an innovative avenue for separating monosaccharides. We modified PIM-1membranes to improve the glucose/xylose separation by incorporating polyethylene glycol monomethyl ether (mPEG). The optimal mPEG (molecular weight: 1000 Da; mass fraction: 2.5 %; solvent: methanol) delivered a xylose separation coefficient of 2.62. With the hybrid membrane of PIM-1-mPEG (50 w.t.%) and hydrophilic Tröger's base polymerer (DMBP-TB, 50 w.t.%), the separation factor for xylose/glucose in an aqueous solution was 2.51 for single-stage running and 11.32 after five-stage running. There are large fractions of micropores for PIM-1-mPEG, and there is difference on solute-membrane interactions for pentose/hexose, which are regarded to be the main driving force for the high pentose/hexose selectivity in methanol. The blending of PIM-1-mPEG and DMBP-TB, integrates the microporosity and hydrophilicity, finally endues the high pentose/hexose selectivity in aqueous solution. These microporous membranes are promising materials for efficiently separating monosaccharides and jnl> small organic molecules while minimizing energy consumption. We established a solid foundation for further exploring microporous membranes for various applications, notably in bioengineering.
研究领域:
生物工程
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材料科学
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分离技术
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产品关联
DOI
高影响因子
EUROPEAN POLYMER JOURNAL
·
2025
·
IF 6.3
Ruiwen Jiao,Dandan Zhang,Wanting Zhu,Xingyuan Hu,Yidi Cai,Xiang Li,Qiukuan Wang,Dandan Ren,Long Wu,Hui Zhou
To solve the problem of poor selectivity of oligosaccharide separation, a new functionalized silica stationary phase, SPTM(THMA-co-MBAAm), was prepared with N-[tris(hydroxymethyl)methyl]acrylamide (THMA) and N,N-methylenebisacrylamide (MBAAm) containing hydroxyl groups as functional monomers. This stationary phase was used to do hydrogen bonding-based oligosaccharide compound separation. The stationary phase SPTM was successfully prepared, and the functional monomers were bonded onto an SiO 2 matrix. The separation of nucleobases and nucleosides on SPTM conformed to the multiple retention mechanism (i.e., a distribution mechanism supplemented by an adsorption mechanism). As a result of the hydrophilic hydroxyl groups on the outermost layer of the phase of stagnation, a layer rich in water was created. The bonds of hydrogen and electromagnetic attraction in addition to repulsion were additionally involved in retention. SPTM was applied to the separation of samples of alginate oligosaccharides with optimized mobile-phase conditions.
研究领域:
生物化学
·
分离科学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Xingyun Zhao,Chengxiao Zhao,Hao Jia,Yifan Niu,Jiangyan Jin,Xiaojian Liu,Zhenyu Li,Xuemei Qin,Ke Li
Hydrothermal carbon (HTC) and modified hydrothermal carbon coated silica microspheres were optimized and applied for the separation of phenolic acids, standard saccharides and natural oligosaccharides in the extract of Traditional Chinese Herbal Medicine (TCM). The materials obtained were characterized by scanning electron microscope (SEM), Fourier-transform infrared spectroscopy (FT-IR), the BET surface area analysis and the zeta potential. The investigation of the synthesized materials and the purchased amide modified silica stationary phases column was performed with a set of test solutes. Both the synthesized HTC coated silica gel (silica@HTC) and the modified silica@HTC column exhibit excellent hydrophilicity. Notably, the poly(ethyleneimine) (PEI) modified silica@HTC column demonstrates superior chromatographic performance in the separation of saccharides. This work showed significant potential in the modification of novel hydrothermal carbon stationary phases for application in separating polar oligosaccharide compositions from TCM extracts and similar sources.
研究领域:
分析化学
·
中药学
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产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2022
·
IF 4.601
Chengjian Wang,Xi Gao,Guiping Gong,Lijuan Man,Qian Wei,Yao Lan,Meifang Yang,Jianli Han,Wanjun Jin,Ming Wei,Linjuan Huang,Zhongfu Wang
Glycomics analysis has been undermined by the lack of structurally defined individual glycans as model compounds. However, it is challenging to prepare individual glycans from natural resources, mainly due to separation difficulties caused by highly diverse structure, complicated mixture form and chromophore-free property of naturally-existing glycans. In this study, we report a simple, universal and low-cost glycan separation strategy, glycoselection, which allows preparation of individual reducing glycans from their mixtures through reversible chromogenic derivatization by hydrazide chemistry in combination with two-dimensional high-performance liquid chromatography (2D-HPLC). Investigations on reaction conditions using lactose and maltodextrin as model glycans showed the feasibility of reversible hydrazide labeling and one-pot hydrazone conversion under mild conditions, the good stability of hydrazone-form derivatives of glycans in solution and the difference among seven selected hydrazine-carrying chromogenic reagents in product yields during glycan labeling and post-column detagging. The 2D-HPLC separation conditions were established on maltodextrin, from which fourteen highly-purified individual reducing oligo-glucans were ultimately obtained. Using this strategy, we also successfully prepared and identified eleven individual neutral reducing N -glycans from chicken ovalbumin and thirteen individual neutral reducing oligosaccharides and eight individual sialylated ones from human milk, demonstrating its good applicability to different types of reducing glycans as well as biological samples. Given the compatibility of individual reducing glycans with almost all of glycan derivatization protocols and analytical techniques of glycans and the potential of the method for larger scale application, this work provides a universal approach to compound-specific analysis of natural glycans and has great significance for glycomics studies.
研究领域:
分析化学
·
糖生物学
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2018
·
IF 3.141
Zhouliangzi Zeng,Jiafei Lyu,Peng Bai,Xianghai Guo
In this work, seven metal–organic frameworks [ZIF-8, MIL-53(Cr), MIL-96(Al), MIL-100(Cr), MIL-100(Fe), MIL-101(Cr), and UiO-66 ] were applied for adsorptive separation of fructose–glucose mixture. UiO-66 exhibited better performance in adsorption capacity and selective adsorption of fructose. The adsorptive process with UiO-66 was further investigated in detail including kinetic, isotherm, and adsorption mechanisms. The rate-determining step analysis based on film diffusion and intraparticle diffusion model suggested that the adsorption process was controlled by multiple steps, which fitted the pseudo-second-order model. The Freundlich model was fitting better than the Langmuir model, which indicated multilayer adsorption on heterogeneous surface. The thermodynamic parameters (ΔG, ΔH, and ΔS) were calculated, indicating that adsorption process on UiO-66 was an endothermic and entropy increment process. UiO-66 can be a promising adsorbent for adsorptive separation of fructose and glucose.
研究领域:
食品科学
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材料科学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2024
·Vol. 25
·期 17
·9465
·
IF 4.9
Xingyun Zhao,Yifan Niu,Chengxiao Zhao,Zhenyu Li,Ke Li,Xuemei Qin
There are great challenges in the field of natural product isolation and purification and in the pharmacological study of oligosaccharide monomers. And these isolation and purification processes are still universal problems in the study of natural products (NPs), traditional Chinese medicine (TCM), omics, etc. The same polymer-modified materials designed for the special separation of oligosaccharides, named Sil-epoxy-PEI and Sil-chloropropyl-PEI, were synthesized via two different methods and characterized by scanning electron microscopy combined with energy spectrum analysis, Fourier transform infrared spectroscopy, thermogravimetric analysis, zeta potential as well as surface area analysis, etc. Several nucleotide/nucleoside molecules with different polarities and selectivities were successfully isolated in our laboratory using stainless-steel columns filled with the synthesized material. In addition, the separation of saccharide probes and oligosaccharides mixtures in water extracts of Morinda officinalis were compared in HILIC mode. The results showed that the resolution of separations for the representative analytes of the Sil-epoxy-PEI column was higher than for the Sil-chloropropyl-PEI column, and the developed stationary phase exhibited improved performance compared to hydrothermal carbon, amide columns and other HILIC materials previously reported.
研究领域:
分析化学
·
天然产物研究
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药物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2025
·
IF 4
Xingyun Zhao,Chengxiao Zhao,Rongfang Wu,Jiangyan Jin,Xiaojian Liu,Zhenwei Zhang,Siyu Yang,Ke Li,Zhenyu Li,Xuemei Qin
The development of core-shell materials for achieving more efficient and rapid chromatographic separations has remained a key objective in separation science. In this study, monodisperse SiO 2 particles (2–4 µm in diameter) were utilized to fabricate core-shell spheres through a combined approach involving a modified sol-gel process and a biphasic system. The synthesis employed 1,6-bis(triethoxysilyl) hexane (BTMSH), 1,2-bis(triethoxysilyl) ethane (BTEE) as a bridging agent with cetyltrimethylammonium bromide (CTAB) acting as a co-template. Notably, the shell thickness could be precisely tailored within the range of 70∼300 nm through systematic optimization of stirring rate, solvent selection, and silica precursor type. The separation of aromatics analytes was evaluated and that baseline separation was achieved for both molecules on all the columns packed n -octadecyltrichlorosilane modified core-shell silica particles with tunable shell thickness. Column efficiencies up to ∼168, 325 plates m -1 were achieved for pyrene on the CS X-E -C18 column ( n -octadecyltrichlorosilane modified core-shell silica). In addition, a hydrophilic interaction liquid chromatography (HILIC) core-shell stationary phase that amide modified core shell silica was prepared (named as CS X-E -AM) via an improved polymerization coating approach and applied for oligosaccharides separation. The results demonstrated that the core-shell spheres were prepared with tailored shell structure and exhibited that the column was stable and could meet the requirement for HPLC separation.
研究领域:
分析化学
·
材料科学
·
色谱技术
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2024
·
IF 8.4
Xiaohui Ju,Hong Gao,Chen Wang,Runtian Li,Wenqi Zhang,Hongjun Ji,Xiao Xiao,Weixing Li,Yujia Tong
A novel nanofiltration (NF) membrane is developed for efficiently separating monosaccharide from disaccharide. A hydrophilic catechol (CT)-Ag interlayer is proposed and co-deposited on a polyacrylonitrile (PAN) substrate, and then interfacial polymerization (IP) of polyethyleneimine (PEI) and trimethyl chloride (TMC) is carried out. The silver nanoparticles obtained through the reduction reaction are used to precisely regulate the unique selective structure with quinone aggregates and polyamides. The composite NF membrane achieved a pure water flux of 31.2 L m −2 h −1 at 0.5 MPa with 81.2 % sucrose rejection and 17.5 % glucose rejection. Performance evaluation in dyes, proteins, and bacteria systems also showcased the great performance of the membrane. Following the filtration of methyl orange and BSA, the flux recovery rates of the PAN/CT-Ag interlayer/PEI + TMC membrane reached 98.7 % and 98.1 %, respectively. The designed structure of silver nanoparticles in membrane enhances the anti-fouling properties through interactions with bacterial proteins. The developed membrane shows promising potential for glucose purification.
研究领域:
生物工程
·
材料科学
·
化学工程
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Tong Chen,Liang Xu,Guangsan Song,Yuan Li,Hong Xu,Hongbin Zhou,Zhen Xiao,Ping Li
Au nanoparticles (Au NPs)-decorated SO 3 H-cofunctionalized porous silica particles was prepared as a novel mixed-mode stationary phase and used in Per aqueous liquid chromatography (PALC) mode. The obtained stationary phase was characterized by elemental analysis, FT-IR spectra, and transmission electron microscopy. To evaluate the retention behavior, the effect of a variety of chromatographic factors on the retention of different types of solutes was investigated. The obtained column exhibited a hydrophobic interaction/cation-exchange mixed-mode retention mechanism, and a typical PALC characteristic was observed in water-rich mobile phases. When the particle size of Au NPs was 10–20 nm, the analytes had a sharp peak shape and the column pressure was also moderate. Compared with the hydrophilic interaction liquid chromatography (HILIC) and C 18 column, some hydrophilic compounds such as six monosaccharides were baseline separated with the enhanced resolution of the obtained column under the PALC mode. Moreover, the efficiency of the Au-S/SO 3 H-SiO 2 -2 column was significantly higher than that of the HILIC column. PALC mode was a green annlysis method and suitable for the replacement of hydrophilic interaction liquid chromatography.
研究领域:
分析化学
·
材料科学
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产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2017
·Vol. 41
·期 22
·13399-13407
·
IF 3.269
Lei Gu,Yun Wang,Juan Han,Lei Wang,Xu Tang,Cheng Li,Liang Ni
We developed an efficient and mild method for the preparation of boronic acid-functionalized magnetic nanoparticles (MNPs), and the selective separation of fructose from a sample solution was demonstrated for the first time. A modified hydrothermal method was chosen to prepare the naked Fe3O4 MNPs, and then the Fe3O4@SiO2 MNPs were synthesized by a sol–gel reaction. After that, the thiol-coated Fe3O4 MNPs were synthesized by a sol–gel reaction with an organosilicon coupling agent. Finally, the thiol groups on the surface of the MNPs served as clickable sites to react with 4-vinylphenylboronic acid (VPBA) via a thiol–ene (TE) click reaction to obtain the final MNPs with a high density of boronic acid ligands immobilized on the surface. It is known that phenylboronic acid ligands on Fe3O4@SiO2@VPBA MNPs can form stable five- or six-membered cyclic esters with cis-diol-containing molecules. In this work, the click-Fe3O4 MNPs with a mean diameter of 195 nm exhibited high selectivity and binding capacity towards fructose. Also, the adsorption efficiency of fructose could reach ≈96% under optimum adsorption conditions. In addition, the adsorption efficiency of fructose was about six times higher than that of glucose in a fructose–glucose aqueous solution, suggesting that the resultant Fe3O4@SiO2@VPBA MNPs had higher affinity for fructose under the interference of competing glucose. Reusability is also an important factor of affinity adsorbents, and the adsorption capacity of Fe3O4@SiO2@VPBA MNPs was virtually unchanged after six cycles of reuse.
研究领域:
分析化学
·
食品科学
·
材料科学
✓ 已验证
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Tong Xin,Mingxuan Chen,Zewei Liu,Ruiwen Luo,Qinglei Xing,Peng Bai,Xianghai Guo,Jiafei Lyu
The past two decades have witnessed the potentials of metal–organic frameworks (MOFs) for the adsorptive separation of difficult mixtures due to their designable porosity and significant surface area. In this work, two Zr 6 -based metal–organic framework materials with different pore sizes, NU-906 and NU-1008, were synthesized and evaluated as adsorbents for fructose/glucose separation, which is very important for sustainable biomass utilization. The two materials were characterized and studied, including the kinetics, isotherms, and thermodynamics by batch adsorption experiments, which can be well described by pseudo-second-order kinetics and Langmuir model (NU-906), Freundlich model (NU-1008), and the adsorption mechanism is described in terms of pore exclusion. The experimental results showed that NU-906 exhibited record-breaking adsorption selectivity towards fructose, whose static selectivity factor was up to 13.7. The NU-906 material with expanded particle size was further studied as filling of the chromatographic column for dynamic adsorption, and the dynamic selectivity factor was about 4.0, which was much higher than the column selectivity factor (∼2.5) used in the industry at present. In addition, three adsorption/regeneration experiments demonstrated its reusability.
研究领域:
化学工程
·
材料科学
·
分离科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）