ChemistrySelect
·
2024
·Vol. 9
·期 11
·e202400114
·
IF 1.9
Jin Liang,Xiaoxin Wang,Dongsheng Yu,XiTao Luo,Xiangfeng Liang,Zhongliang Su,Qinghui Shou
Graphical Self-supported osmotic membranes were prepared by blending PIM-1-mPEG, which was obtained by modifying PIM-1 with polyethylene glycol monomethyl ether (mPEG), and hydrophilic Tröger's base polymer (DMBP-TB). The membranes utilized microporous molecular sieves and the differences in solute-membrane interactions to achieve high pentose/hexose selectivity in aqueous solutions. Monosaccharides are vital building blocks in bioengineering applications; however, their extraction from intricate mixtures is challenging and uses substantial amounts of energy. Polymers of intrinsic microporosity (PIMs) offer an innovative avenue for separating monosaccharides. We modified PIM-1membranes to improve the glucose/xylose separation by incorporating polyethylene glycol monomethyl ether (mPEG). The optimal mPEG (molecular weight: 1000 Da; mass fraction: 2.5 %; solvent: methanol) delivered a xylose separation coefficient of 2.62. With the hybrid membrane of PIM-1-mPEG (50 w.t.%) and hydrophilic Tröger's base polymerer (DMBP-TB, 50 w.t.%), the separation factor for xylose/glucose in an aqueous solution was 2.51 for single-stage running and 11.32 after five-stage running. There are large fractions of micropores for PIM-1-mPEG, and there is difference on solute-membrane interactions for pentose/hexose, which are regarded to be the main driving force for the high pentose/hexose selectivity in methanol. The blending of PIM-1-mPEG and DMBP-TB, integrates the microporosity and hydrophilicity, finally endues the high pentose/hexose selectivity in aqueous solution. These microporous membranes are promising materials for efficiently separating monosaccharides and jnl> small organic molecules while minimizing energy consumption. We established a solid foundation for further exploring microporous membranes for various applications, notably in bioengineering.
研究领域:
生物工程
·
材料科学
·
分离技术
✓ 已验证
产品关联
DOI
高影响因子
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
·
2017
·Vol. 244
·期 5
·851-860
·
IF 1.664
Xu Dan,Liu Hong,Jin Cheng-yu,Cao Chun-mei,Li Wen-gang,Zeng Fan-kui,Zhao Yu-ci,Liu Gang
Hongmei is a new potato variety developed in recent year, which is suitable for raw eating as a kind of fruit. In this study, sensory evaluations, nutritional components, morphology characteristics of starch granules, phenolic compounds, sugars, glycoalkaloids, and amino acid composition of potato Hongmei were investigated. The results show that Hongmei be suitable for raw eating mainly based on three reasons: (1) possess a juicy characteristic and lower earthy smell, with a distinctive fruity flavor of fresh cucumber; (2) the average particle size of starch from Hongmei is 18.31 ± 7.71 μm, which is smaller than the commercial potato starch significant ( p < 0.01); (3) with a content of 653.64 ± 83.25 mg/100 g of fructose, which is similar to the sweet potato be suitable for raw eating. The results also show that Hongmei had high concentrations of crude protein, total ascorbic acid, but lower starch content. The total glycoalkaloids content in the flesh is only 5.52 mg/kg, which implied that it is safe for raw eating. However, due to the total glycoalkaloids in the Hongmei peel is 358.88 mg/kg, which is higher than the safe standard of 200 mg/kg, it is recommended to peel when eating raw.
研究领域:
食品科学
·
植物学
✓ 已验证
DOI
高影响因子
CHINESE JOURNAL OF CATALYSIS
·
2024
·
IF 15.7
Lulu Sun,Shiyang Liu,Taifeng Liu,Dongqiang Lei,Nengchao Luo,Feng Wang
ABSTRACT Photocatalytic decomposition of sugars is a promising way of providing H 2 , CO, and HCOOH as sustainable energy vectors. However, the production of C 1 chemicals requires the cleavage of robust C−C bonds in sugars with concurrent production of H 2 , which remains challenging. Here, the photocatalytic activity for glucose decomposition to HCOOH, CO (C 1 chemicals), and H 2 on Cu/TiO 2 was enhanced by nitrogen doping. Owing to nitrogen doping, atomically dispersed and stable Cu sites resistant to light irradiation are formed on Cu/TiO 2 . The electronic interaction between Cu and nitrogen ions originates valence band structure and defect levels composed of N 2 p orbit, distinct from undoped Cu/TiO 2 . Therefore, the lifetime of charge carriers is prolonged, resulting in the production of C 1 chemicals and H 2 with productivities 1.7 and 2.1 folds that of Cu/TiO 2 . This work provides a strategy to design coordinatively stable Cu ions for photocatalytic biomass conversion.
研究领域:
化学工程
·
材料科学
·
可再生能源
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE
·
2019
·Vol. 144
·期 6
·420-428
·
IF 0.829
Yu Wang,Haobo Yang,Shuai Zhong,Xin Liu,Tong Li,Chengwen Zong
Sugar and organic acid contents as well as sugar-to-acid ratio of fruit are important factors affecting fruit quality and processing. In this study, we compared sugar and organic acid contents of Vaccinium uliginosum (bog bilberry) fruit harvested from 100 plants across 10 wild populations growing in the Changbai Mountains of China. Correlation of sugar and acid contents with the spatial distribution of these populations was accessed. Germplasm with high sugar and low acid with potential for use in future breeding was also identified. Results showed that sugar and organic acid contents varied among different V. uliginosum populations in the Changbai Mountains. Fructose (18.60–38.54 mg·g−1) and glucose (15.77–35.08 mg·g−1) were the main sugars, whereas quinic (16.06–20.97 mg·g−1), citric (10.39–14.96 mg·g−1), and malic (4.28–7.47 mg·g−1) acids were the main organic acids in V. uliginosum. The first population collected in the Dongfanghong forest farm (lat. 42°00′N, long. 128°03′E) showed the highest average sugar content and sugar-to-acid ratio among the sampled populations. Moreover, total sugar content and sugar-to-acid ratio of Sample 7—Population 1, which is located at Manjiang forest farm (lat. 41°09′N, long. 127°05′E) was much higher than the other 99 samples, making this sample representative of important germplasm for breeding. Altitude and latitude were important environmental factors affecting sugar and organic acid contents. Fructose, glucose, and total sugar contents were positively correlated with altitude and negatively correlated with latitude. Oxalic acid content and sugar-to-acid ratio were positively correlated with altitude, and there was no significant correlation between organic acid contents and latitude.
研究领域:
植物学
·
园艺学
✓ 已验证
DOI
高影响因子
EUROPEAN POLYMER JOURNAL
·
2025
·
IF 6.3
Ruiwen Jiao,Dandan Zhang,Wanting Zhu,Xingyuan Hu,Yidi Cai,Xiang Li,Qiukuan Wang,Dandan Ren,Long Wu,Hui Zhou
To solve the problem of poor selectivity of oligosaccharide separation, a new functionalized silica stationary phase, SPTM(THMA-co-MBAAm), was prepared with N-[tris(hydroxymethyl)methyl]acrylamide (THMA) and N,N-methylenebisacrylamide (MBAAm) containing hydroxyl groups as functional monomers. This stationary phase was used to do hydrogen bonding-based oligosaccharide compound separation. The stationary phase SPTM was successfully prepared, and the functional monomers were bonded onto an SiO 2 matrix. The separation of nucleobases and nucleosides on SPTM conformed to the multiple retention mechanism (i.e., a distribution mechanism supplemented by an adsorption mechanism). As a result of the hydrophilic hydroxyl groups on the outermost layer of the phase of stagnation, a layer rich in water was created. The bonds of hydrogen and electromagnetic attraction in addition to repulsion were additionally involved in retention. SPTM was applied to the separation of samples of alginate oligosaccharides with optimized mobile-phase conditions.
研究领域:
生物化学
·
分离科学
✓ 已验证
产品关联
DOI
高影响因子
BIOMACROMOLECULES
·
2021
·
IF 6.988
Ronghua Yin,Lutan Zhou,Na Gao,Lisha Lin,Huifang Sun,Dingyuan Chen,Ying Cai,Zhili Zuo,Kaifeng Hu,Shengxiong Huang,Jikai Liu,Jinhua Zhao
Glycosaminoglycans (GAGs) are conserved polysaccharides composed of linear repeating disaccharides and play crucial roles in multiple biological processes in animal kingdom. However, saccharide-branched GAGs are rarely found, except the fucose-branched one from sea cucumbers. There was conjecture about the presence of disaccharide-branched GAG since 30 years ago, though not yet confirmed. Here, we report a GAG containing galactose-fucose branches from Thelenota ananas. This unique branch was confirmed as d-Gal4S(6S)-α1,2-l-Fuc3S by structural elucidation of oligosaccharides prepared from T. ananas GAG. Bioassays indicated that oligomers with a larger degree of polymerization exhibited a potent anticoagulation by targeting the intrinsic tenase. Heptasaccharide was proven as the minimum fragment retaining the anticoagulant potential and showed 92.6% inhibition of venous thrombosis in vivo at sc. of 8 mg/kg with no obvious bleeding risks. These results not only solve a long-standing question about the presence of disaccharide-branched GAG in Holothuroidea, but open up new opportunities to develop safer anticoagulants.
研究领域:
生物化学
·
海洋生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Hossain M. Zabed,Suely Akter,Yuehui Tian,Mudasir A. Dar,Junhua Yun,Mei Zhao,Arthur J. Ragauskas,Jia Li,Xianghui Qi
This study evaluated microbial systems and technological approaches to configure the whole slurry co-fermentation process for ethanol biosynthesis from a novel stover system rich in sugars. Two approaches, namely separate and simultaneous hydrolysis and co-fermentation (SHCF and SSCF, respectively), were investigated using Escherichia coli monoculture and E. coli- yeast coculture. The SSCF with E. coli monoculture produced 32.75 g/L ethanol, representing only 44.87% yield, which left 65.13 g/L of total sugars unconverted and exhibited limited xylose consumption. Subsequently, a coculture-based SHCF significantly enhanced sugar consumption, leading to increase in ethanol yield and concentration to 66.94% and 48.86 g/L, respectively. Nevertheless, xylose utilization remained minimal due to the preference for glucose and the inhibitory effects of certain compounds. Thereafter, modification of the medium composition by supplementing betaine and sodium metabisulfite improved ethanol production to 53.18 g/L by reducing the toxic effects of inhibitors. Finally, a dual-phase SSCF (DP-SSCF) was explored by allowing the consumption of sugars from the pretreated slurry in the first phase, followed by concurrent cellulose hydrolysis and utilization of the resulting glucose in the second phase. This strategy increased ethanol titer to 63.14 g/L, with 84.3% yield and 0.88 g/L/h productivity.
研究领域:
生物工程
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2016
·Vol. 8
·期 7
·1572-1576
·
IF 1.915
Xiaoming Jiang,Junchao Xu,Youlai Zhang,Hongying Wang,Lintao Zeng,Yue Zhang
A colorimetric and ratiometric fluorescent probe was developed for the detection of sulfite in sugar. The probe underwent a Michael addition reaction with sulfite, which resulted in significant changes in fluorescence ratios. The probe displayed a fast response (within 30 s) and high selectivity towards sulfite over other anions and bio-thiols. The fluorescence intensity ratios were linearly related to the concentration of SO32− ranging from 0 to 15 μM, and the limit of detection was determined to be as low as 12 nM. The probe was employed to determine the concentration of sulfite in sugar with good recovery.
研究领域:
分析化学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Xingyun Zhao,Chengxiao Zhao,Hao Jia,Yifan Niu,Jiangyan Jin,Xiaojian Liu,Zhenyu Li,Xuemei Qin,Ke Li
Hydrothermal carbon (HTC) and modified hydrothermal carbon coated silica microspheres were optimized and applied for the separation of phenolic acids, standard saccharides and natural oligosaccharides in the extract of Traditional Chinese Herbal Medicine (TCM). The materials obtained were characterized by scanning electron microscope (SEM), Fourier-transform infrared spectroscopy (FT-IR), the BET surface area analysis and the zeta potential. The investigation of the synthesized materials and the purchased amide modified silica stationary phases column was performed with a set of test solutes. Both the synthesized HTC coated silica gel (silica@HTC) and the modified silica@HTC column exhibit excellent hydrophilicity. Notably, the poly(ethyleneimine) (PEI) modified silica@HTC column demonstrates superior chromatographic performance in the separation of saccharides. This work showed significant potential in the modification of novel hydrothermal carbon stationary phases for application in separating polar oligosaccharide compositions from TCM extracts and similar sources.
研究领域:
分析化学
·
中药学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2022
·
IF 4.601
Chengjian Wang,Xi Gao,Guiping Gong,Lijuan Man,Qian Wei,Yao Lan,Meifang Yang,Jianli Han,Wanjun Jin,Ming Wei,Linjuan Huang,Zhongfu Wang
Glycomics analysis has been undermined by the lack of structurally defined individual glycans as model compounds. However, it is challenging to prepare individual glycans from natural resources, mainly due to separation difficulties caused by highly diverse structure, complicated mixture form and chromophore-free property of naturally-existing glycans. In this study, we report a simple, universal and low-cost glycan separation strategy, glycoselection, which allows preparation of individual reducing glycans from their mixtures through reversible chromogenic derivatization by hydrazide chemistry in combination with two-dimensional high-performance liquid chromatography (2D-HPLC). Investigations on reaction conditions using lactose and maltodextrin as model glycans showed the feasibility of reversible hydrazide labeling and one-pot hydrazone conversion under mild conditions, the good stability of hydrazone-form derivatives of glycans in solution and the difference among seven selected hydrazine-carrying chromogenic reagents in product yields during glycan labeling and post-column detagging. The 2D-HPLC separation conditions were established on maltodextrin, from which fourteen highly-purified individual reducing oligo-glucans were ultimately obtained. Using this strategy, we also successfully prepared and identified eleven individual neutral reducing N -glycans from chicken ovalbumin and thirteen individual neutral reducing oligosaccharides and eight individual sialylated ones from human milk, demonstrating its good applicability to different types of reducing glycans as well as biological samples. Given the compatibility of individual reducing glycans with almost all of glycan derivatization protocols and analytical techniques of glycans and the potential of the method for larger scale application, this work provides a universal approach to compound-specific analysis of natural glycans and has great significance for glycomics studies.
研究领域:
分析化学
·
糖生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2016
·
IF 4.052
Xin Wang,Lihui Zhang,Jingli Wu,Weiqi Xu,Xiaoqin Wang,Xin Lü
Although pre-column derivatization with n-propylamine and acetic anhydride combined with the gas chromatograph was a useful method for the analysis of monosaccharide composition, failure often occurs because of lack of detail information on the mechanism as well as the operating key point in derivatization process. In this study, the key points in the derivatization (lactonization time, the amount of n-propylamine and acetic anhydride) were investigated and optimized to improve the method. Under the optimal conditions, the derivatives of seven neutral monosaccharides and two uronic acids were simultaneouly obtained, after which they were well separated and detected by GC. It was also found that all derivatives of monosaccharides were stable even stored for 20 days. The linearity, sensitivity, precision, reproducibility and recovery rate of the improved method were evaluated. Thereafter, five polysaccharide samples from different sources were analyzed to validate the improved method.
研究领域:
分析化学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2018
·
IF 3.141
Zhouliangzi Zeng,Jiafei Lyu,Peng Bai,Xianghai Guo
In this work, seven metal–organic frameworks [ZIF-8, MIL-53(Cr), MIL-96(Al), MIL-100(Cr), MIL-100(Fe), MIL-101(Cr), and UiO-66 ] were applied for adsorptive separation of fructose–glucose mixture. UiO-66 exhibited better performance in adsorption capacity and selective adsorption of fructose. The adsorptive process with UiO-66 was further investigated in detail including kinetic, isotherm, and adsorption mechanisms. The rate-determining step analysis based on film diffusion and intraparticle diffusion model suggested that the adsorption process was controlled by multiple steps, which fitted the pseudo-second-order model. The Freundlich model was fitting better than the Langmuir model, which indicated multilayer adsorption on heterogeneous surface. The thermodynamic parameters (ΔG, ΔH, and ΔS) were calculated, indicating that adsorption process on UiO-66 was an endothermic and entropy increment process. UiO-66 can be a promising adsorbent for adsorptive separation of fructose and glucose.
研究领域:
食品科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）