INTERNATIONAL JOURNAL OF FOOD PROPERTIES
·
2022
·
IF 3.388
Duo Liu,Zenghui Shi,Siqi Wang,Liyuan Zhai,Dongxia Gou,Jun Zhao,Yanbo Hu
Black soybean hull, as main by-production in black bean processing, has significant biological activity. In this study, the polysaccharide from black soybean hull (BSPS-H) was obtained by enzymatic hydrolysis, the yield was 5.3%, and its chemical composition and structural composition were further studied. The results shown that BSPS-H was mainly composed of Mannose, Galactose, and Glucose (molar ratio = 48.1: 29.7: 15.8), and might be existed in the form of pyran ring in α/β-linkage type. The anti-oxidant activity shown that BSPS-H had a certain capacity of anti-oxidation in a concentration-dependent manner. Among them, it has the best scavenging effect on DPPH free radicals. When the polysaccharide concentration was 10 mg/ml, the scavenging rate reached 94.37%. The results of anti-fatigue effect indicated that BSPS-H could prolong the duration of exhaustive swimming in mice, increased the contents of liver glycogen, reduced the content of blood urea nitrogen (BUN), enhanced the activity of lactate dehydrogenase (LDH) and reduced the accumulation of lactic acid (LA). This study provides a fundamental basis for the development and use of BSPS-H in medicine and functional foods.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
Research
·
2023
·
IF 11
Guangchun Song,Jia Xu,Hong Zhong,Qi Zhang,Xin Wang,Yitong Lin,Scott P. Beckman,Yunbo Luo,Xiaoyun He,Jin-Cheng Li,Kunlun Huang,Nan Cheng
The enzyme-mimicking catalytic activity of single-atom nanozymes has been widely used in tumor treatment. However, research on alleviating metabolic diseases, such as hyperglycemia, has not been reported. Herein, we found that the single-atom Ce-N4-C-(OH)2 (SACe-N4-C-(OH)2) nanozyme promoted glucose absorption in lysosomes, resulting in increased reactive oxygen species production in HepG2 cells. Furthermore, the SACe-N4-C-(OH)2 nanozyme initiated a cascade reaction involving superoxide dismutase-, oxidase-, catalase-, and peroxidase-like activity to overcome the limitations associated with the substrate and produce •OH, thus improving glucose intolerance and insulin resistance by increasing the phosphorylation of protein kinase B and glycogen synthase kinase 3β, and the expression of glycogen synthase, promoting glycogen synthesis to improve glucose intolerance and insulin resistance in high-fat diet-induced hyperglycemic mice. Altogether, these results demonstrated that the novel nanozyme SACe-N4-C-(OH)2 alleviated the effects of hyperglycemia without evident toxicity, demonstrating its excellent clinical application potential.
研究领域:
纳米技术
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生物化学
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代谢疾病
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药理学
✓ 已验证
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2022
·
IF 5.895
Jialin Liu,Yanli Wang,Xiaoxiao Li,Zhengyu Jin,Birte Svensson,Yuxiang Bai
Glycogen-like glucan (GnG) is a unique hyperbranched polysaccharide nanoparticle which is drawing increasing attention due to its biodegradability and abundant short branches that can be functionalized. Because starch and GnG are both composed of glucose residues and have similar glucosidic bonds, GnG could be fabricated by sucrose phosphorylase, α-glucan phosphorylase, and branching enzymes from starch primers and sucrose. In this study, high-amylose starch, normal starch, and waxy corn starch were used as primers to synthesize GnG, and their impact on the fine structure of GnG was investigated. Structural analysis indicated that with increasing content of amylopectin in the starch primer, the proportion of short chains in GnG decreased, and the degree of β-amylolysis and α-amylolysis was enhanced. Amylose in the primer contributed to a compact and homogeneous structure of GnG, while amylopectin triggered the formation of branch points with a more open distribution. These findings provide a new strategy for regulating the fine structure of GnG.
研究领域:
生物化学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
Foods
·
2021
·Vol. 10
·期 3
·529
·
IF 4.35
Jinsong Wang,Lanming Chen
Probiotics are considered as desirable alternatives to antibiotics because of their beneficial effects on the safety and economy of farm animals. The protectant can ensure the viability of probiotics, which is the prerequisite of the beneficial effects. The objective of this study was to evaluate the effects of a novel nano-protectant containing trehalose, skim milk powder, phytoglycogen nanoparticles, and nano-phytoglycogen Pickering emulsions on the viability ofLactobacillus caseiK17 under different conditions. The results indicated that the optimal concentration of the carbohydrate substrate was determined to be 10% skim milk powder (w/w) instead of trehalose. The combination of 10% skim milk powder (w/w), 1% phytoglycogen nanoparticles (w/w), and 10% Pickering emulsions (w/w) was selected as the optimal component of the protectant. Trilayer protectants with an optimal component had a more significant protective effect on the bacteria than that of the monolayer and bilayer protectants, or the control in feed storage, freeze-drying, and simulated gastrointestinal environment. A scanning electron microscope was used to monitor the morphological characteristics of the protectants for different layers onL. casei. In conclusion, the trilayer protectant exhibited a substantial effect onL. caseiduring storage and consumption, which could be used in the feed and functional food.Keywords:Lactobacillus casei;protectants;probiotics;phytoglycogen nanoparticle;pickering emulsions
研究领域:
微生物学
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食品科学
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DOI
PubMed
高影响因子
STARCH-STARKE
·
2015
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IF 1.677
Xuan Liang,Shuyun Wu,Xianyue Ren,Daping Quan,Li-Ming Zhang,Yubing Deng,Liqun Yang
A series of hyperbranched glycogen derivatives conjugated with different contents of cationic 3-(dimethylamino)-1-propylamine residues, named DMAPA-Glyp derivatives, were synthesized at room temperature. Resonance light scattering (RLS) spectroscopy was used to investigate the complexation behavior and efficacy of the DMAPA-Glyp derivatives and plasmid DNA (pDNA). Results indicated that the DMAPA-Glyp derivatives and pDNA molecules began to aggregate and form complexes at a weight ratio of 2. Following further increases in the DMAPA-Glyp derivatives (w/w >2), pDNA was condensed to form compact complexes. RLS spectroscopy is thus a useful tool for studying the complexation behavior and efficacy of cationic polymers with nucleic acid molecules. Circular dichroism analysis further revealed that the conformation of pDNA transformed from B-form to C-form and A-form during the complexation process.
研究领域:
生物化学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2024
·
IF 5.3
Xiaofei Ma,Chengchen Zhang,Liangliang Cai
The enantioseparation of chiral drugs and the potential synergistic effect between deep eutectic solvents (DESs) and polysaccharide chiral selector (glycogen) were first investigated in capillary electrophoresis (CE). The results showed that significantly improved separations of six model drugs were observed compared with single glycogen system. After completing the optimization of separation conditions, the mechanism of DESs improving enantioseparations was studied by means of Statistical Product and Service Solutions (SPSS) analysis, ultraviolet spectroscopy and nuclear magnetic resonance (NMR) methods. It was the first time that synergistic systems based on DESs and polysaccharide chiral selector were established, and DESs were effective auxiliary additives to enhance the enantioselectivity of the glycogen system in CE.
研究领域:
分析化学
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药学
✓ 已验证
产品关联
DOI
高影响因子
VIBRATIONAL SPECTROSCOPY
·
2020
·
IF 1.917
Ren Hu,Cheng Zong,Long-xiang Lin,Ting Wei,Qian Cheng,Yan-xia Jiang,Chang-jian Lin,Bin Ren,Zhong-qun Tian
A facile Fourier transform infrared (FTIR) spectroscopy is developed to detect live cell activities in the transmission mode. With the use of an O ring to aid cell culture on optical windows, live cell activities can be semi-quantitatively investigated using a commercially available demountable liquid device by FTIR spectroscopy in the transmission mode. Cellular volume adaptation to osmolarity was observed by FTIR spectra for PC12 cells. The chemical information upon growth of human cervical cancer cell line, HeLa cells, were investigated by FTIR at interval incubation time. Glycogen enrichment concurring with cell proliferation was illustrated, but the dissipation of glycogen upon subculturing is to be understood. Further comparison of the CaSki cells with its counterpart Ect1/E6E7 cells revealed bands features for the discrimination of the two cell types. These results demonstrate that the developed protocol can be complementary to microincubators and facilitate chemical analyses of live cellular activities by FTIR spectroscopy in the transmission mode.
研究领域:
生物化学
·
细胞生物学
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医学检测
✓ 已验证
DOI
高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 7
·1-10
·
IF 6.408
Sun Chenglong,Li Lu,Liu Jialin,Du Yun,Peng Yueyi,Xie Qingji
The photoelectrochemical immunoassay of glycogen phosphorylase BB (GPBB) was studied. A methyl orange/TiO 2 nanorod heterojunction was constructed on a fluorine-doped tin oxide electrode by hydrothermal synthesis, calcination, and chemical adsorption. A sandwich immune structure consisting of GPBB as the first antibody, GPBB, and a CdS@mesoporous silica-ascorbic acid (AA)–GPBB as secondary antibody composite was constructed on each of the selected well surfaces of a 96-well microplate. By adding mercaptoethylamine to structurally destroy the secondary antibody composite and release the electron donor AA, the amplification of photocurrent, and thus the “off–on” photoelectrochemical biosensing of GPBB were realized. The use of the 96-well microplate provides good reproducibility of the assembled immune structures and eliminates the possible effect of the photogenerated hole–induced protein oxidation on the photocurrent. The relevant electrodes and materials were characterized by electrochemistry, UV–vis diffuse reflectance spectra, Fourier transform infrared spectroscopy, X-ray diffractometer, scanning electron microscopy/energy dispersive spectroscopy, transmission electron microscopy and BET method. Under the optimal conditions, the photocurrent was linear with the logarithm of GPBB concentration from 0.005 to 200 ng mL −1 and with a limit of detection of 1.7 pg mL −1 ( S / N = 3). Satisfactory results were obtained in the analysis of real serum samples. Graphical abstract A sandwich immune structure consisting of GPBB first antibody, GPBB, and a CdS@mesoporous silica-ascorbic acid (AA)–GPBB secondary antibody composite was constructed on each of the selected well surfaces of a 96-well microplate. By adding mercaptoethylamine to structurally destroy the secondary antibody composite and release the electron donor AA, the amplification of photocurrent, and thus the “off–on” photoelectrochemical biosensing of GPBB were realized.
研究领域:
生物化学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CANADIAN ENTOMOLOGIST
·
2025
·
IF 1.1
Chunfu Zhang,Kaifei Guo,Song Yang,Zhen Chen
In this study, changes in physiological characteristics of Coridius chinensis (Hemiptera: Dinidoridae) during diapause and post-diapause development period were determined. The moisture content of C. chinensis at the beginning of diapause was significantly lower than that at any other stage, and the moisture content during post-diapause development period was significantly higher than that from October to December 2021 and in February and April of the following year. The fat content gradually declined over time. The glycogen content remained at lower levels during diapause but rose sharply during the post-diapause development period, when it became significantly higher than that during diapause. The trehalose content gradually declined in the early stages of diapause but rose greatly in the middle stage, followed by a gradual decline in the late stages and a significant increase during the post-diapause development period. The protein content was at lower levels in the early stages of diapause, significantly lower than that in the middle and late stages of diapause and that during post-diapause development period. The results indicated significant differences in changes in the moisture, fat, carbohydrate, and protein contents between the diapause and post-diapause development periods, with obvious stage characteristics. This study provides a scientific basis for further research on the diapause physiology of C. chinensis.
研究领域:
昆虫生理学
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滞育生物学
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代谢生理学
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昆虫生物化学
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发育生物学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Jing Zhu,Yi Gao,Xingfei Li,Cheng Lu,Xing Zhou,Long Chen,Chao Qiu,Zhengyu Jin,Jie Long
Maltogenic α-amylase (MA, EC 3.2.1.133), which shows high thermal stability, can cleave long starch chains linked by α-1,4 glycosidic bonds. And glycogen branching enzyme (GBE, EC 2.4.1.18) can increase the branching degree of starch. At high temperatures, Elevated temperatures enhanced starch solubilization, improving the accessibility of both MA and GBE to glucan chains. Therefore, ungelatinized normal corn starch (NCS) emulsion (20 % w /w, dry basis) was first pretreated with α-amylase (MA) at 70 °C for 2 h, followed by enzymatic modification using the thermostable Pyrococcus horikoshii OT3 GBE mutants (H415W and T508K) for an additional 10 h modification. The physicochemical properties of the modified starch were determined by scanning electron microscopy and X-ray diffraction, etc. The results demonstrated that the dual enzyme treatment decreased rapid digestibility by 23.07 % and retrograde enthalpy by 5.51 J/g compared to NCS. This study provides guidance for the industrial enzymatic preparation of highly branched starch.
研究领域:
食品科学
·
生物技术
·
碳水化合物化学
·
酶学
·
淀粉加工
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LIFE SCIENCES
·
2020
·
IF 3.647
Weiyi Zhang,Jie Gao,Fukui Shen,Xiaoyao Ma,Zhihua Wang,Xiaotao Hou,Erwei Hao,Yuanyuan Hou,Gang Bai
Aims Hepatic glucose metabolism involves a variety of catabolic and anabolic pathways, and the dynamic balance of glucose metabolism is regulated in response to environmental and nutritional changes. The molecular mechanism of glucose metabolism in liver is complex and has not been fully elucidated so far. In this study, we hope to elucidate the target and mechanism of cinnamaldehyde (CA) in regulating glucose metabolism. Materials and methods Molecular image tracing and magnetic capture in combination with an alkynyl-CA probe (Al-CA) was used to show CA covalently binds to α-enolase (ENO1) in both mouse liver and HepG2 cells. Accurate metabolic flow assays subsequently demonstrated that the utilization of glycogenic amino acids and the biosynthesis of tricarboxylic acid (TCA) cycle intermediates were strengthened, which was detected using nontargeted and targeted metabolomics analyses. Key findings Our study shows that CA covalently bonds with ENO1, which affects the stability and activity of ENO1 and changes the dynamic balance of glucose metabolism. The interruption of gluconeogenic reflux by ENO1 enhanced TCA cycle, and eventually led to a decrease in blood glucose and the improvement of mitochondrial efficiency. Significance These results provide a detailed description of how CA maintains the dynamic balance of glucose utilization and improves energy metabolism.
研究领域:
药理学
·
代谢
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
·
IF 3.909
Yujuan Li,Yizhou Xin,Fangxue Xu,Mengmeng Zheng,Xiaozhi Xi,Xiaowei Cui,Hui Cao,Hong Guo,Chunchao Han
The maca polysaccharides optimal extraction conditions were obtained by using response surface methodology (RSM) method and the anti-fatigue activity of maca polysaccharides (MCP) was explored. The maca polysaccharides extract yield of RSM could reach 9.97 mg/g by using the model predicts, and the total sugar and protein purity were 61.00% and 4.46% with the further isolation process, respectively. And the monosaccharide compositions obtained by gas chromatograph (GC) were composed of rhamnose (rha), glucose (glc), galactose (gal) with the ratio of 2.34:10.21:1.00. Furthermore, the anti-fatigue activity was evaluated by the swimming parameter, biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD)), the result indicated that the low-dose maca polysaccharides group had the significant anti-fatigue activity.
研究领域:
药学
·
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）