ELECTROANALYSIS
·
2014
·Vol. 26
·期 4
·856-863
·
IF 2.502
Jiuyang Wu,Yuqing Miao,Xiaocai Liang,Zhuoyuan Yang,Yang Yang,Ruizhuo Ouyang
Metallic Bi and Ni were co-deposited onto the surface of glass carbon electrode (GCE) from the electrolyte solution containing their respective nitrate to fabricate a Bi/Ni alloy modified GCE (Bi/Ni-GCE). The purpose is to study the influence of Bi 3+ on the deposition of Ni and that of deposited Bi on the electrocatalytic performance of Ni to glucose in alkali solution. The results show that both redox signal of Ni(OH) 2 /NiOOH and Ni(OH) 2 /NiOOH mediated electrocatalysis to glucose is remarkably increased in the presence of Bi. It seems that there is a synergistic effect between Bi and Ni on each other’s redox electrochemistry. It’s possible that the firstly deposited Bi on GCE surface helps to the following nucleation and growth of Ni, leading to the deposition of more metallic Ni on GCE surface. An extremely attractive feature of Bi/Ni-GCE is reflected by the fast response time to the electrocatalytic oxidation of glucose. The electrode nearly responses immediately after glucose is added and it reaches a steady-state level within only 2 seconds, demonstrating a good electrocatalytic property of Bi/Ni-GCE. The calibration plot is linear over the wide concentration range of 0–5.8 mM with a sensitivity of 33.96 µA/mM and a correlation coefficient of 0.9985. The detection limit of the glucose was found to be 0.59 µM at a signal-to-noise ratio of 3. The fabricated Bi/Ni-GCE was successfully employed to analyze the glucose level in blood samples, exhibiting high accuracy, strong resistance against inference and good reliability in the practical applications.
研究领域:
电化学
·
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Catalysis Science & Technology
·
2024
·
IF 4.4
Qingxiu Wang,Weisong Liu,Lijing Chang,Kuncheng Zhang,Yanbing Shen,Lingling Zhang
L-guluronic acid, a patented drug with immunomodulatory and anti-inflammatory properties, is traditionally sourced from natural seaweed species or synthesized chemically. Both methods suffer from low selectivity and environmental concerns and there is a high demand to develop a green and efficient approach for L-guluronic acid production. Herein, we successfully developed a two-step enzymatic route to synthesize L-guluronic acid from D-glucose by two oxidases, glucose oxidase (GOx) and galactose oxidase (GOase). Due to the sluggish kinetics of GOase in catalyzing the oxidation of D-gluconic acid, rational design was implemented. Through molecular docking and iterative saturation mutagenesis, a variant, GOase T406R/K330R/Y329F, was obtained. The variant exhibited a remarkable 13-fold higher oxidation activity towards D-gluconic acid, reaching 7.8 U mg-1. The substitutions synergistically switch the D-gluconic acid molecule to a favorable conformation and facilitate the specific activation of the primary alcohol group at C6 position. In a cascade reaction, complete conversion of 40 mM D-glucose to L-guluronic acid was achieved in 2 h with 100% selectivity, presenting a promising and green bio-manufacturing strategy for the scalable production of L-guluronic acid.
研究领域:
生物催化
·
酶工程
·
绿色化学
·
药物
✓ 已验证
产品关联
DOI
高影响因子
FUEL
·
2024
·
IF 6.7
Guojun Liu,Shaoshuai Wang,Caiming Zhou,Qiang Zhao,Jiaxue Hu,Zhenzheng Gui,Yuhui Chen,Yong Huang,Peng Zhang,Fenfen Wang
Formic acid is a versatile and promising value-added chemical derived from the chemoselective transformation of renewable biomass resources. However, achieving a high production rate of formic acid remains a pivotal and enormous challenge. Herein, we synthesized a sequence of BiVO x catalysts based on vanadium-promotion by a facile and cost-effective strategy for selective oxidation of biomass-derived xylose in water under O 2 atmosphere. Particularly, the resultant Bi 1 V 2 O x exhibited a remarkably enhanced catalytic activity, higher selectivity toward formic acid, and excellent recyclability, giving a yield of 75.8 % and a production rate of formic acid (9.22 mol FA mol V −1 h −1 ) at 170 °C within only 20 min. Such pronounced improved performance was largely attributed to the synergistic effect between V and Bi species, with higher surface area, abundant surface acidity, as well as stronger adsorption energy, meanwhile efficiently suppressing the formation of undesired by-products. This study demonstrates that the promotion of V in BiVO x leads to more efficient catalytic oxidation of xylose to formic acid, and with further driven research that proved great potential for generating formic acid from renewable biomass resources for future industrial application.
研究领域:
化学工程
·
可再生资源转化
·
催化材料科学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2018
·
IF 4.244
Guobin Mao,Chen Liu,Mingyuan Du,Yuwei Zhang,Xinghu Ji,Zhike He
Galactose oxidase is a copper-activated enzyme and have a vital role in metabolism of galactose. Much of the work is focused on determining the amount of galactose in the blood rather than measuring the amount of galactose oxidase to urge the galactosemia patients to restrict milk intake. Here, a simple and effective method was developed for Cu 2+ and copper-activated enzyme detection based on homogenous alloyed CdZnTeS quantum dots (QDs). Meso − 2,3-dimercaptosuccinic acid (DMSA) was used as the reducing agent for preparing QDs and the highest quantum yield of CdZnTeS QDs was 69.4%. In addition, the as-prepared CdZnTeS QDs show superior fluorescence properties, such as good photo-/chemical stability. The DMSA was the surface ligand of the QDs, containing abundant -SH and -COOH, thus the surface ligands have a high affinity with Cu 2+ . Therefore, this developed probe can be applied for Cu 2+ and galactose oxidase detection and shows a good sensitivity in the buffer. Then, this probe was successfully used for Cu 2+ and galactose oxidase detection in real samples with the satisfactory results. The proposed fluorescence quenching strategy gives a new and simple insight for enzyme assay without the enzyme-catalyzed reaction.
研究领域:
生物化学
·
医学检测
·
纳米技术
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Weiwei Mao,Bin Cai,Zhizhen Ye,Jingyun Huang
A glucose oxidase (GOx) based p-NiO/n-Bi 4 Ti 3 O 12 sandwich heterojunction was fabricated and explored as a glucose electrochemical sensor. The constructed GOx/p-NiO/n-Bi 4 Ti 3 O 12 sandwich structure effectively prevents the leakage of GOx. Dandelion-like Bi 4 Ti 3 O 12 nanowires were directly grown on the titanium foil substrates, and dense NiO nanoparticles were deposited by employing pulsed laser deposition at room temperature. Amperometric measurements towards glucose were carried out, and efficient direct electron transfer was successfully achieved. The enzyme biosensor showed a linear range from 20 μM to 3.55 mM (R = 0.999) with an ultrahigh sensitivity of 215 μA mM −1 cm −2 , response time within 2 s, low detection limit of 1.26 μM (S/N = 3) and high specificity. The apparent Michaelis-Menten constant (Km app) was estimated to be 0.22 mM. Intercrossed nanowires and interconnected pores favor the electrons transfer and the massive GOx loading. Binder-free feature leads to a seamless connection between Bi 4 Ti 3 O 12 nanowires and the substrate. Co-catalysis of GOx and p-NiO/n-Bi 4 Ti 3 O 12 heterojunction plays a synergetic role in gaining performance boosts. The GOx based p-NiO/n-Bi 4 Ti 3 O 12 heterojunction biosensor remedies the limitation of enzyme-based glucose sensor and be potentially applied in practice.
研究领域:
生物医学工程
·
材料科学
·
分析化学
✓ 已验证
DOI
高影响因子
GREEN CHEMISTRY
·
2020
·Vol. 22
·期 8
·2588-2597
·
IF 9.48
Xintong Meng,Zengyong Li,Di Li,Yiming Huang,Jiaojiao Ma,Chuanfu Liu,Xinwen Peng
Catalytic and selective oxidation of monosaccharides into value-added sugar acids is highly desirable from both scientific and industrial perspectives. Here, we report a hierarchical porous carbon (NC-3) supported gold catalyst (Au/NC-3) and its application in the base-free aerobic oxidation of D-glucose and D-xylose into value-added sugar acids under mild conditions (100 °C). The catalyst exhibited excellent catalytic performance with the yields of D-gluconic acid and D-xylonic acid reaching 97.62% and 98.76%, respectively. We studied the effect of the ball-milling process on the hierarchical porous preparation, which is critical to the formation of the fibrous structure during the pyrosis process. This specific structure provides active sites for Au adsorption. DFT calculation revealed that the Au NPs prefer to anchor on pyridinic N sites; meanwhile, the initial reactants and essential intermediates are found to be well stabilized by Au/NC-3. Moreover, upon recycling, gold was stable toward the recovery procedure. In this work, we provide a green method for an improved generally selective oxidation of D-glucose and D-xylose using Au/NC-3 as an efficient heterogeneous catalyst.
研究领域:
化学
·
催化科学
✓ 已验证
DOI
高影响因子
ELECTROCHIMICA ACTA
·
2019
·
IF 5.383
Lili Xiao,Qing Zhao,Lin Jia,Qi Chen,Jin Jiang,Qiyu Yu
Nickel-based nanoparticles have been widely used in detection of glucose due to its multiple oxidation states and high stability in alkaline environment. In this work, amorphous nickel phosphate was supported by Co-based zeolitic imidazolate framework (ZIF-67) through ion exchange to prepare Ni 3 (PO 4 ) 2 @ZIF-67 composites as the electrode material . In comparison to glassy carbon electrode (GCE), Ni 3 (PO 4 ) 2 /GCE and ZIF-67/GCE, the Ni 3 (PO 4 ) 2 @ZIF-67/GCE showed significantly enhanced performances in electrocatalytic oxidation of glucose. The great improvement on glucose oxidation current could be ascribed to the unique structure of Ni 3 (PO 4 ) 2 @ZIF-67, the mutual promotion between Ni(III)/Ni(II) and Co(IV)/Co(III) interfacial redox reactions and increased surface areas. Under optimum conditions, as-prepared Ni 3 (PO 4 ) 2 @ZIF-67/GCE showed an ultra-high sensitivity of 2783 μA mM −1 cm −2 with a wide linear ranged from 1.0 μM to 4.0 mM, and a low limit detection of 0.7 μM at a signal-to-noise ratio of 3. Moreover, Ni 3 (PO 4 ) 2 @ZIF-67/GCE were successfully applied to detect glucose of human serum with good recoveries (92–109%), which indicated that Ni 3 (PO 4 ) 2 @ZIF-67 can found application in the clinical detection.
研究领域:
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2017
·Vol. 9
·期 44
·6286-6292
·
IF 1.9
Lingxian Ye,Guangying Zhao,Wenchao Dou
Developing portable and low-cost methods for quantitative detection of Cronobacter sakazakii (C. sakazakii) from powdered infant formula plays a significant role in controlling and preventing a range of serious neonatal infections. Here, we reported a novel methodology for C. sakazakii detection using a low-cost, simple, and widely accessible personal glucose meter (PGM) as the readout. The electrochemical immunoassay strategy was based on antibody and glucose oxidase coated silica nanoparticles (SiNP–GOX–IgG) and antibody functionalized silica-coated magnetic nanoparticles (MNP–IgG). In the process of detecting a positive sample, a sandwich immunocomplex was formed between the MNP–IgG, C. sakazakii and SiNP–GOX–IgG. Then, the above sandwich composites were dispersed in glucose solution, and the concentration of glucose was measured using the PGM. Under optimal conditions, a linear relationship between the decrease of glucose concentration and the logarithm of C. sakazakii concentration was obtained in the concentration range from 9.0 × 102 to 9.0 × 107 CFU mL−1 with a detection limit of 4.2 × 101 CFU mL−1 (S/N = 3). This simple immunoassay holds great potential as an efficient, sensitive and selective point-of-care testing (POCT) tool for C. sakazakii detection.
研究领域:
微生物学
·
分析化学
·
医学诊断
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2025
·
IF 12.5
Nannan Zhang,Hongkai Tian,Wenyi Zong,Qingao Fan,Jinlian Hua,Jinyi Wang,Qin Tu
The management of diabetic wounds represents a pivotal aspect of clinical care, as the hyperglycemic milieu within these wounds significantly impedes the healing process and predisposes to severe complications. Moreover, uncontrolled hemorrhage post-injury further exacerbates the impediment to wound healing. Cryogels, characterized by their porous architecture, exhibit exceptional capabilities in absorbing blood exudates, facilitating rapid hemostatic and augmenting wound healing. In this study, we utilized a sulfobetaine-modified chitosan and graphene oxide (SCG) composite as the cryogel matrix, which was subsequently impregnated with zeolitic imidazolate framework-67 (ZIF-67) to enhance the cryogel's adsorption capacity and optimizing hemostatic properties. Concurrently, glucose oxidase (GO X ) was encapsulated within ZIF-67, leveraging the framework's propensity to degrade under the acidic conditions prevalent in diabetic wounds. This controlled release of GO X in the hyperglycemic and acidic environment of diabetic wounds reduces local glucose concentrations, thereby fostering wound healing. The hemostatic efficacy of the SCG/ZIF-67/GO X cryogel was rigorously assessed through three distinct animal hemorrhage models. Furthermore, in a full-thickness skin wound model in type I diabetic mice, the SCG/ZIF-67/GO X cryogel demonstrated significant efficacy in promoting the healing of diabetic wounds. These findings highlight the SCG/ZIF-67/GO X cryogel's potential as a promising therapeutic intervention for rapid hemostasis and diabetic wounds healing.
研究领域:
生物材料学
·
医学
·
药剂学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2025
·
IF 9.7
Xiaoyu Wang,Chunzhe Xu,Hantao Tian,Yu Pang,Jie Lv,Meng Li
Ferroptosis, a non-apoptotic programmed cell death modality, has been recognized as an emerging therapeutic target for cancer treatment, particularly with the rapid advancements in bionanotechnology. However, the insufficient intracellular Fe 2+ ions and low reactive oxygen species (ROS) production severely restrict the efficacy of ferroptosis at tumor sites. Herein, a pH-responsive multifunctional nanoplatform (p-COF@GOx-FeS@HA/ART) was constructed to achieve efficient tumor ferroptosis through self-supplied Fe 2+ ions and amplified ROS. In this system, the large specific surface area and mesoporous structure enabled the porphyrin-based covalent organic frameworks (p-COFs) to act as scaffolds and drug carriers for enhancing the catalytic activity of glucose oxidase-stabilized ferrous sulfide nanodots (GOx@FeS) and encapsulation of artesunate (ART). By oxidizing glucose (Glu) in tumor cells, GOx not only consumed Glu for starvation therapy but also promoted intracellular acidity and supplied hydrogen peroxide (H 2 O 2 ) in the tumor microenvironment (TME), which facilitated the FeS-mediated chemodynamic therapy (CDT) as well as the release of hydrogen sulfide (H 2 S) for accelerating the ROS generation. Moreover, the lowered acidic TME could simultaneously trigger the release of ART and Fe 2+ ions, thus exacerbating ART-mediated ferroptosis. Due to its photothermal and photodynamic behavior, the nanoplatform under laser irradiation could generate ROS storms in tumor cells for high-performance ferroptosis therapy, which was demonstrated both in cancer cells and tumor-bearing mice. This work provides a promising strategy for the simple construction of a multifunctional nanoplatform with TME-responsive and self-triggered ferroptosis, showing great potential in cascade amplification of ferroptosis therapy.
研究领域:
肿瘤学
·
生物材料学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Functional Materials Letters
·
2021
·
IF 2.17
Biao Wang,Ya Liu,Xu Huai,Yuqing Miao
In order to develop non-noble metal-based electrocatalysts for glucose oxidation, the Ni-doped, urchin-like Bi 2 S 3 particles were prepared by a solvothermal method using the solvent of ethylene glycol/H 2 O. The obtained products were characterized by scanning electron microscopy, transmission electron microscopy, X-ray photoelectron spectroscopy and X-ray diffraction. The background signal from capacitance current is relatively low and the electrocatalytic oxidation current of glucose relatively high due to the urchin-like nanostructure of Bi 2 S 3 particles and high surface area where the presence of Bi also improves the electrocatalytic performance of Ni I I /Ni I I I shift.
研究领域:
材料科学
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Yang Zhao,Ze-Hua Huang,Hui-Ming Zhou,Ke-Xue Zhu,Xiao-Na Guo,Wei Peng
Hexose oxidase was a feasible prevention for the dark spots in the fresh wet noodle sheets (FWNS). The chemical mechanism that hexose oxidase recucing the melanins of dark spots was discussed basis on the UPLC-TOF-MS analysis of the polyphenol oxidase (PPO)-catechol system. In the process of PPO browning, hexose oxidase catalyzed the oxidation of o -benzoquinone derivatives and their oligomers, hindering the formation of melanins. Hexose oxidase was efficient in FWNS with low ash content when water addition was 24%~44% or pH range was 4 ~ 7.5. Hexose oxidase could inhubit dark spots in the presence of 10 metal ions. The recommended addition amount was 40 ~ 60 ppm, by which the dark spots could be compolitely inhibited. Hexose oxidase was also suitable for wholewheat and oat FWNS, Δ L 6d of wholewheat and oat FWNS were reduced by 4 and 7.98, respectively.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）